Enzymatic repair of DNA, 1. Purification of two enzymes involved in the excision of thymine dimers from ultraviolet-irradiated DNA.

abstract：:Two anti-tumor monoclonal antibodies, L6 (anticarcinoma) and 1F5 (anti-B lymphoma), were covalently linked to alkaline phosphatase (AP), forming conjugates that could bind to the surface of antigen-positive tumor cells. The conjugates were capable of converting a relatively noncytotoxic prodrug, etoposide phosphate (E...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Senter PD,Saulnier MG,Schreiber GJ,Hirschberg DL,Brown JP,Hellström I,Hellström KE

更新日期：1988-07-01 00:00:00

abstract：:The rat brain-derived neurotropic factor (BDNF) gene consists of four 5' exons linked to separate promoters and one 3' exon encoding the prepro-BDNF protein. To gain insights into the regulation of BDNF mRNA expression, probes specific for the different 5' exons were used to study the expression of BDNF mRNA in the br...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Metsis M,Timmusk T,Arenas E,Persson H

更新日期：1993-10-01 00:00:00

abstract：:Chromosome-specific gene regulation is known thus far only as a mechanism to equalize the transcriptional activity of the single male X chromosome with that of the two female X chromosomes. In Drosophila melanogaster, a complex including the five Male-Specific Lethal (MSL) proteins, "paints" the male X chromosome, med...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Larsson J,Chen JD,Rasheva V,Rasmuson-Lestander A,Pirrotta V

更新日期：2001-05-22 00:00:00

abstract：:Angiogenin, a potent inducer of neovascularization in the chicken chorioallantoic membrane and rabbit cornea, promotes endothelial cell invasion of Matrigel basement membrane. A transformed bovine aortic endothelial cell line, GM 7373, is 5 times more invasive when cultured in the presence of 1 microgram of bovine ang...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu G,Riordan JF,Vallee BL

更新日期：1994-12-06 00:00:00

abstract：:We previously purified a 48-kDa protein (p48) that specifically reacts with an antiserum directed against the 12 carboxyl-terminal amino acids of the c-myc gene product. Using an antiserum directed against the purified p48, we have cloned a cDNA from a human expression library. This cDNA hybrid-selects an mRNA that tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giallongo A,Feo S,Moore R,Croce CM,Showe LC

更新日期：1986-09-01 00:00:00

abstract：:The genome of the defective avian tumor virus MH2 was identified as a RNA of 5.7 kilobases by its presence in different MH2-helper virus complexes and its absence from pure helper virus, by its unique fingerprint pattern of RNase T1-resistant (T1) oligonucleotides that differed from those of two helper virus RNAs, and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duesberg PH,Vogt PK

更新日期：1979-04-01 00:00:00

abstract：:During primate evolution, a major factor in lengthening life-span and decreasing age-specific cancer rates may have been improved protective mechanisms against oxygen radicals. We propose that one of these protective systems is plasma uric acid, the level of which increased markedly during primate evolution as a conse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ames BN,Cathcart R,Schwiers E,Hochstein P

更新日期：1981-11-01 00:00:00

abstract：:Chlorarachniophytes are amoeboid algae with unusual chloroplasts. Instead of the usual two membranes that surround the chloroplasts of plants, green algae, and red algae, the chloroplasts of chlorarachniophytes have four bounding membranes. The extra membranes may reflect an unusual origin of chlorarachniophyte chloro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McFadden GI,Gilson PR,Hofmann CJ,Adcock GJ,Maier UG

更新日期：1994-04-26 00:00:00

abstract：:A Jurassic salamander, Beiyanerpeton jianpingensis (gen. et sp. nov.), from a recently found site in western Liaoning Province, China is the earliest known record of Salamandroidea. As a Late Jurassic record of the group, it extends the range of the clade by ~40 Ma. The Late Jurassic taxon is neotenic and represented ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao KQ,Shubin NH

更新日期：2012-04-10 00:00:00

abstract：:The effects of pyrophosphate on RNA binding and ATPase activities of Escherichia coli transcription termination factor rho have been studied. Mutant rho-115 protein has a temperature-sensitive RNA-dependent ATPase activity due to the thermolability of binding to RNA [Kent, R.B. & Guterman, S.K. (1981) Fed. Proc. Fed. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kent RB,Guterman SK

更新日期：1982-07-01 00:00:00

abstract：:Proteases constitute the largest enzyme family, yet their biological roles are obscured by our rudimentary understanding of their cellular substrates. There are 12 human caspases that play crucial roles in inflammation and cell differentiation and drive the terminal stages of cell death. Recent N-terminomics technolog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Julien O,Zhuang M,Wiita AP,O'Donoghue AJ,Knudsen GM,Craik CS,Wells JA

更新日期：2016-04-05 00:00:00

abstract：:Professional antigen-presenting dendritic cells (DCs) are critical in regulating T cell immune responses at both systemic and mucosal sites. Many Lactobacillus species are normal members of the human gut microflora and most are regarded as safe when administered as probiotics. Because DCs can naturally or therapeutica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohamadzadeh M,Olson S,Kalina WV,Ruthel G,Demmin GL,Warfield KL,Bavari S,Klaenhammer TR

更新日期：2005-02-22 00:00:00

abstract：:We report a comprehensive cartography of selective responses to visual letters and words in the human ventral occipito-temporal cortex (VOTC) with direct neural recordings, clarifying key aspects of the neural basis of reading. Intracerebral recordings were performed in a large group of patients (n = 37) presented wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lochy A,Jacques C,Maillard L,Colnat-Coulbois S,Rossion B,Jonas J

更新日期：2018-08-07 00:00:00

abstract：:Inducible cAMP early repressor (ICER) is a transcriptional repressor, which, because of alternate promoter use, is generated from the 3' region of the cAMP response modulator (Crem) gene. Its expression and nuclear occurrence are elevated by high cAMP levels in naturally occurring regulatory T cells (nTregs). Using tw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vaeth M,Gogishvili T,Bopp T,Klein M,Berberich-Siebelt F,Gattenloehner S,Avots A,Sparwasser T,Grebe N,Schmitt E,Hünig T,Serfling E,Bodor J

更新日期：2011-02-08 00:00:00

abstract：:Taking advantage of a fluorescent Ca(2+) indicator selectively targeted to the trans-Golgi lumen, we here demonstrate that its Ca(2+) homeostatic mechanisms are distinct from those of the other Golgi subcompartments: (i) Ca(2+) uptake depends exclusively on the activity of the secretory pathway Ca(2+) ATPase1 (SPCA1),...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lissandron V,Podini P,Pizzo P,Pozzan T

更新日期：2010-05-18 00:00:00

abstract：:Immune-mediated pulmonary diseases are a significant public health concern. Analysis of leukocyte behavior in the lung is essential for understanding cellular mechanisms that contribute to normal and diseased states. Here, we used two-photon imaging to study neutrophil extravasation from pulmonary vessels and subseque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kreisel D,Nava RG,Li W,Zinselmeyer BH,Wang B,Lai J,Pless R,Gelman AE,Krupnick AS,Miller MJ

更新日期：2010-10-19 00:00:00

abstract：:The mouse glucocorticoid receptor-interacting protein (GRIP1) is a member of the ERAP160 family of nuclear receptor (NR) coactivators (including SRC-1 and TIF2) which function as bridging proteins between ligand-activated NRs bound to cognate hormone-response elements (HREs) and the transcription initiation apparatus ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walfish PG,Yoganathan T,Yang YF,Hong H,Butt TR,Stallcup MR

更新日期：1997-04-15 00:00:00

abstract：:30S ribosomal proteins from Bacillus stearothermophilus (B. proteins) have been fractionated and characterized with respect to their ability to replace various E. coli 30S proteins (E. proteins) in the E. coli 30S ribosome reconstitution system. The functional counterparts of all the E. proteins, except S1, S6, S9, an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Higo K,Held W,Kahan L,Nomura M

更新日期：1973-03-01 00:00:00

abstract：:Excess activation of glutamate receptors and production of free radicals including nitric oxide may result in severe and irreversible damage to the mammalian central nervous system (CNS), but endogenous defense systems that protect neurons from these insults are poorly understood. Here, we purified and isolated a neur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kume T,Asai N,Nishikawa H,Mano N,Terauchi T,Taguchi R,Shirakawa H,Osakada F,Mori H,Asakawa N,Yonaga M,Nishizawa Y,Sugimoto H,Shimohama S,Katsuki H,Kaneko S,Akaike A

更新日期：2002-03-05 00:00:00

abstract：:The expression of the human HSP70 gene is induced by a wide range of physiological stresses, including exposure to heat shock and heavy metals, or under nonstress conditions, such as in response to serum stimulation. We have previously demonstrated that in either case the regulated expression is at the primary level o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu BJ,Kingston RE,Morimoto RI

更新日期：1986-02-01 00:00:00

abstract：:Lantibiotics are ribosomally synthesized, posttranslationally modified peptide antibiotics. The biosynthetic gene cluster for microbisporicin, a potent lantibiotic produced by the actinomycete Microbispora corallina containing chlorinated tryptophan and dihydroxyproline residues, was identified by genome scanning and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Foulston LC,Bibb MJ

更新日期：2010-07-27 00:00:00

abstract：:We previously identified rat8 in the pathway involved in epithelial cell differentiation that occurs in the rat mammary gland at pregnancy when tubules and alveoli are formed. rat8, which encodes an IFN-inducible membrane protein, is the rat homologue of the mouse gene fragilis. By differential detergent extraction an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zucchi I,Prinetti A,Scotti M,Valsecchi V,Valaperta R,Mento E,Reinbold R,Vezzoni P,Sonnino S,Albertini A,Dulbecco R

更新日期：2004-02-17 00:00:00

abstract：:DNA origami enables the precise fabrication of nanoscale geometries. We demonstrate an approach to engineer complex and reversible motion of nanoscale DNA origami machine elements. We first design, fabricate, and characterize the mechanical behavior of flexible DNA origami rotational and linear joints that integrate s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marras AE,Zhou L,Su HJ,Castro CE

更新日期：2015-01-20 00:00:00

abstract：:The prgB gene encodes the surface protein, Asc10, which mediates cell aggregation, resulting in high-frequency conjugative transfer of the pheromone-inducible tetracycline-resistance plasmid pCF10 in Enterococcus faecalis. Messenger RNA analysis by Northern blot hybridization and primer extension indicates that prgB t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung JW,Dunny GM

更新日期：1992-10-01 00:00:00

abstract：:Exchange proteins directly activated by cAMP (Epac1 and Epac2) have been recently recognized as key players in β-adrenergic-dependent cardiac arrhythmias. Whereas Epac1 overexpression can lead to cardiac hypertrophy and Epac2 activation can be arrhythmogenic, it is unknown whether distinct subcellular distribution of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pereira L,Rehmann H,Lao DH,Erickson JR,Bossuyt J,Chen J,Bers DM

更新日期：2015-03-31 00:00:00

abstract：:The tumor suppressor Smad4/Dpc4 is a transcription activator that binds specific DNA sequences and whose nuclear localization is induced after exposure to type beta transforming growth factor-like cytokines. We explored an inducible system in which Smad4 protein is activated by translocation to the nucleus when cell l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dai JL,Bansal RK,Kern SE

更新日期：1999-02-16 00:00:00

abstract：:Sugar compartmentation into vacuoles of higher plants is a very important physiological process, providing extra space for transient and long-term sugar storage and contributing to the osmoregulation of cell turgor and shape. Despite the long-standing knowledge of this subcellular sugar partitioning, the proteins resp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aluri S,Büttner M

更新日期：2007-02-13 00:00:00

abstract：:The crystal structure of the decanucleotide d(CGCAATTGCG)2 has been solved by a combination of molecular replacement and heavy-atom procedures and has been refined to an R factor of 20.2% at 2.7 A. It is not a fully base-paired duplex but has a central core of eight Watson-Crick base pairs flanked by unpaired terminal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spink N,Nunn CM,Vojtechovsky J,Berman HM,Neidle S

更新日期：1995-11-07 00:00:00

abstract：:Complement receptors (CRs), expressed notably on myeloid and lymphoid cells, play an essential function in the elimination of complement-opsonized pathogens and apoptotic/necrotic cells. In addition, these receptors are crucial for the cross-talk between the innate and adaptive branches of the immune system. CR3 (also...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bajic G,Yatime L,Sim RB,Vorup-Jensen T,Andersen GR

更新日期：2013-10-08 00:00:00

abstract：:Analysis, by the recovery of specific genetic "markers," of the effects of DNase I, physical shear, and temperature shock on DNA-cell complexes demonstrates that sequential attachment of both ends of bacteriophage SP82G DNA to Bacillus subtilis precedes entry of the DNA molecule into the cell, and that each attachment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams GL,Green DM

更新日期：1972-06-01 00:00:00