Initiation factor 2-dependent ribosomal binding of N-formylmethionyl-transfer RNA without added guanosine triphosphate.

abstract：:The biosynthetic origin of the 10,000 molecular weight neurophysins, carriers of the peptide hormones oxytocin and vasopressin, has been studied by cell-free synthesis, Poly(A)-RNA was isolated from bovine hypothalamus and translated in a wheat germ system containing (35)S- or (3)H-labeled amino acids. A number of uni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giudice LC,Chaiken IM

更新日期：1979-08-01 00:00:00

abstract：:The genes encoding the variable (V) region of the B-cell antigen receptor (BCR) are assembled from V, D (diversity), and J (joining) elements through a RAG-mediated recombination process that relies on the recognition of recombination signal sequences (RSSs) flanking the individual elements. Secondary V(D)J rearrangem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun A,Novobrantseva TI,Coffre M,Hewitt SL,Jensen K,Skok JA,Rajewsky K,Koralov SB

更新日期：2015-02-03 00:00:00

abstract：:Green fluorescent protein (GFP) is widely used as a reporter gene in both prokaryotes and eukaryotes. However, the fluorescence levels of wild-type GFP (wtGFP) are not bright enough for fluorescence-activated cell sorting or flow cytometry. Several GFP variants were generated that are brighter or have altered excitati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson MT,Tjioe IM,Lorincz MC,Parks DR,Herzenberg LA,Nolan GP,Herzenberg LA

更新日期：1996-08-06 00:00:00

abstract：:Stable structures of icosahedral symmetry can serve numerous functional roles, including chemical microencapsulation and delivery of drugs and biomolecules, epitope presentation to allow for an efficient immunization process, synthesis of nanoparticles of uniform size, observation of encapsulated reactive intermediate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olson AJ,Hu YH,Keinan E

更新日期：2007-12-26 00:00:00

abstract：:We have transferred the human gene for hypoxanthine-guanine phosphoribosyltransferase (HPRT, EC 2.4.2.8; IMP:pyrophosphate phosphoribosyltransferease) via isolated metaphase chromosomes from human HeLa S3 cells into murine A9 cells which lack functional murine HPRT activity, using the technique of McBride and Ozer (Pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willecke K,Ruddle FH

更新日期：1975-05-01 00:00:00

abstract：:Endothelial cells (ECs) not only are important for oxygen delivery but also act as a paracrine source for signals that determine the balance between tissue regeneration and fibrosis. Here we show that genetic inactivation of flow-induced transcription factor Krüppel-like factor 2 (KLF2) in ECs results in reduced liver...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manavski Y,Abel T,Hu J,Kleinlützum D,Buchholz CJ,Belz C,Augustin HG,Boon RA,Dimmeler S

更新日期：2017-04-11 00:00:00

abstract：:AB5 bacterial toxins and polyomaviruses induce membrane curvature as a mechanism to facilitate their entry into host cells. How membrane bending is accomplished is not yet fully understood but has been linked to the simultaneous binding of the pentameric B subunit to multiple copies of glycosphingolipid receptors. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kabbani AM,Raghunathan K,Lencer WI,Kenworthy AK,Kelly CV

更新日期：2020-06-30 00:00:00

abstract：:Methane emissions in the Arctic are important, and may be contributing to global warming. While methane emission rates from Arctic lakes are well documented, methods are needed to quantify the relative contribution of active layer groundwater to the overall lake methane budget. Here we report measurements of natural t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paytan A,Lecher AL,Dimova N,Sparrow KJ,Kodovska FG,Murray J,Tulaczyk S,Kessler JD

更新日期：2015-03-24 00:00:00

abstract：:We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vieira P,de Waal-Malefyt R,Dang MN,Johnson KE,Kastelein R,Fiorentino DF,deVries JE,Roncarolo MG,Mosmann TR,Moore KW

更新日期：1991-02-15 00:00:00

abstract：:Tracheal antimicrobial peptide (TAP) is a member of the beta-defensin family of antibiotic peptides found in the tracheal mucosa of the cow. TAP gene expression in the bovine airway is inducible by lipopolysaccharide and inflammatory mediators, suggesting that it functions to protect the upper airway from infection. L...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yarus S,Rosen JM,Cole AM,Diamond G

更新日期：1996-11-26 00:00:00

abstract：:BDF1 [(C57BL/6 X DBA/2)F1] mice were primed with alum-absorbed ovalbumin, and their spleen cells were cultured with ovalbumin to activate antigen-primed T cells. The activated T cells were then propagated in interleukin 2-containing medium in the presence or absence of affinity-purified glycosylation-inhibiting factor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iwata M,Ishizaka K

更新日期：1987-04-01 00:00:00

abstract：:Recessive Stargardt's macular degeneration is a blinding disease of children caused by mutations in the ABCA4 (ABCR) gene. Mice with a knockout mutation in abcr accumulate toxic lipofuscin pigments in ocular tissues, similar to affected humans. The major fluorophore of lipofuscin is the bis-retinoid, N-retinylidene-N-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Radu RA,Mata NL,Bagla A,Travis GH

更新日期：2004-04-20 00:00:00

abstract：:Adeno-associated virus (AAV) vector delivery of an Apaf-1-dominant negative inhibitor was tested for its antiapoptotic effect on degenerating nigrostriatal neurons in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of Parkinson's disease. The wild-type caspase recruitment domain of Apaf-1 was used as a dom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mochizuki H,Hayakawa H,Migita M,Shibata M,Tanaka R,Suzuki A,Shimo-Nakanishi Y,Urabe T,Yamada M,Tamayose K,Shimada T,Miura M,Mizuno Y

更新日期：2001-09-11 00:00:00

abstract：:The covalent linkage of supramolecular monomers provides a powerful strategy for constructing dynamic polymeric materials whose properties can be readily tuned either by the selection of monomers or the choice of functional linkers. In this strategy, the stabilities of the supramolecular monomers and the reactions use...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang M,Li S,Yan X,Zhou Z,Saha ML,Wang YC,Stang PJ

更新日期：2016-10-04 00:00:00

abstract：:A series of spontaneous suppressors to the temperature-sensitive phenotype of the single-stranded DNA-binding protein mutation ssb-1 were isolated. A genomic library of EcoRI fragments from one of these suppressor strains was prepared by using pBR325 as the cloning vector. A 10.0-kilobase class of inserts was identifi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruben SM,VanDenBrink-Webb SE,Rein DC,Meyer RR

更新日期：1988-06-01 00:00:00

abstract：:Sepiapterin reductase (7,8-dihydrobiopterin: NADP+ oxidoreductase, EC 1.1.1.153) catalyzes the terminal step in the biosynthetic pathway for tetrahydrobiopterin, the cofactor necessary for aromatic amino acid hydroxylation. We report here the isolation of a cDNA clone for rat liver sepiapterin reductase. The cDNA has ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Citron BA,Milstien S,Gutierrez JC,Levine RA,Yanak BL,Kaufman S

更新日期：1990-08-01 00:00:00

abstract：:Whereas target-specific drugs are available for treating ERBB2-overexpressing and hormone receptor-positive breast cancers, no tailored therapy exists for hormone receptor- and ERBB2-negative ("triple-negative") mammary carcinomas. Triple-negative tumors account for 15% of all breast cancers and frequently harbor defe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rottenberg S,Jaspers JE,Kersbergen A,van der Burg E,Nygren AO,Zander SA,Derksen PW,de Bruin M,Zevenhoven J,Lau A,Boulter R,Cranston A,O'Connor MJ,Martin NM,Borst P,Jonkers J

更新日期：2008-11-04 00:00:00

abstract：:Two-dimensional Raman-terahertz (THz) spectroscopy is presented as a multidimensional spectroscopy directly in the far-IR regime. The method is used to explore the dynamics of the collective intermolecular modes of liquid water at ambient temperatures that emerge from the hydrogen-bond networks water forming. Two-dime...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Savolainen J,Ahmed S,Hamm P

更新日期：2013-12-17 00:00:00

abstract：:Although de novo protein design is an important endeavor with implications for understanding protein folding, until now, structures have been determined for only a few 25- to 30-residue designed miniproteins. Here, the NMR solution structure of a complex 73-residue three-helix bundle protein, alpha3D, is reported. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walsh ST,Cheng H,Bryson JW,Roder H,DeGrado WF

更新日期：1999-05-11 00:00:00

abstract：:Cell mechanics plays a role in stem cell reprogramming and differentiation. To understand this process better, we created a genetically encoded optical probe, named actin-cpstFRET-actin (AcpA), to report forces in actin in living cells in real time. We showed that stemness was associated with increased force in actin....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo J,Wang Y,Sachs F,Meng F

更新日期：2014-12-09 00:00:00

abstract：:Zn2+ is critical for the functional and structural integrity of cells and contributes to a number of important processes including gene expression. It has been shown that NO exogenously applied via NO donors resulting in nitrosative stress leads to cytoplasmic Zn2+ release from the zinc storing protein metallothionein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spahl DU,Berendji-Grün D,Suschek CV,Kolb-Bachofen V,Kröncke KD

更新日期：2003-11-25 00:00:00

abstract：:The nitrogen isotopic composition (15N/14N) of forested ecosystems varies systematically worldwide. In tropical forests, which are elevated in 15N relative to temperate biomes, a decrease in ecosystem 15N/14N with increasing rainfall has been reported. This trend is seen in a set of well characterized Hawaiian rainfor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Houlton BZ,Sigman DM,Hedin LO

更新日期：2006-06-06 00:00:00

abstract：:The embryonic development of the optic nerve of the zebrafish, Brachydanio rerio, was studied by three-dimensional computer reconstruction from serial section electron micrographs. Growing fibers from retinal ganglion cells had growth cones in contact with more mature fibers from adjacent cell bodies. In the observed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bodick N,Levinthal C

更新日期：1980-07-01 00:00:00

abstract：:How individuals make decisions has been a matter of long-standing debate among economists and researchers in the life sciences. In economics, subjects are viewed as optimal decision makers who maximize their overall reward income. This framework has been widely influential, but requires a complete knowledge of the rew...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kubanek J

更新日期：2017-08-08 00:00:00

abstract：:Clostridium ljungdahlii is an anaerobic homoacetogen, able to ferment sugars, other organic compounds, or CO(2)/H(2) and synthesis gas (CO/H(2)). The latter feature makes it an interesting microbe for the biotech industry, as important bulk chemicals and proteins can be produced at the expense of CO(2), thus combining...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Köpke M,Held C,Hujer S,Liesegang H,Wiezer A,Wollherr A,Ehrenreich A,Liebl W,Gottschalk G,Dürre P

更新日期：2010-07-20 00:00:00

abstract：:Endocrine therapy resistance invariably develops in advanced estrogen receptor-positive (ER+) breast cancer, but the underlying mechanisms are largely unknown. We have identified C-terminal SRC kinase (CSK) as a critical node in a previously unappreciated negative feedback loop that limits the efficacy of current ER-t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao T,Li W,Wang X,Xu H,Yang J,Wu Q,Huang Y,Geradts J,Jiang P,Fei T,Chi D,Zang C,Liao Q,Rennhack J,Andrechek E,Li N,Detre S,Dowsett M,Jeselsohn RM,Liu XS,Brown M

更新日期：2018-07-31 00:00:00

abstract：:Voltage gating of hyperpolarization-activated cation (HCN) channels is potentiated by direct binding of cAMP to a cytoplasmic cAMP-sensing domain (CSD). When unliganded, the CSD inhibits hyperpolarization-dependent opening of the HCN channel gate; cAMP binding relieves this autoinhibition so that opening becomes more ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wicks NL,Wong T,Sun J,Madden Z,Young EC

更新日期：2011-01-11 00:00:00

abstract：:The ability to modulate the collective properties of correlated electron systems at their interfaces and surfaces underpins the burgeoning field of "designer" quantum materials. Here, we show how an electronic reconstruction driven by surface polarity mediates a Stoner-like magnetic instability to itinerant ferromagne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mazzola F,Sunko V,Khim S,Rosner H,Kushwaha P,Clark OJ,Bawden L,Marković I,Kim TK,Hoesch M,Mackenzie AP,King PDC

更新日期：2018-12-18 00:00:00

abstract：:Understanding molecular mechanisms in the context of living cells requires the development of new methods of in vivo biochemical analysis to complement established in vitro biochemistry. A critically important molecular mechanism is genetic recombination, required for the beneficial reassortment of genetic information...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cockram CA,Filatenkova M,Danos V,El Karoui M,Leach DR

更新日期：2015-08-25 00:00:00

abstract：:The ribosomal RNA genes (rDNA) of Tetrahymena thermophila macronucleus exist as free linear 21-kilobase molecules that contain replication origins and telomeres. A mutation in this gene confers resistance to the antibiotic paromomycin. We have isolated rDNA from such a mutant (strain p2f), microinjected it into the ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tondravi MM,Yao MC

更新日期：1986-06-01 00:00:00