Estrogen-regulated feedback loop limits the efficacy of estrogen receptor-targeted breast cancer therapy.

abstract：:The assembly and composition of human excision nuclease were investigated by electrophoretic mobility shift assay and DNase I footprinting. Individual repair factors or any combination of up to four repair factors failed to form DNA-protein complexes of high specificity and stability. A stable complex of high specific...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wakasugi M,Sancar A

更新日期：1998-06-09 00:00:00

abstract：:With the goal of developing techniques for DNA insertional mutagenesis in zebrafish, we established procedures for rapidly obtaining and injecting large numbers of fertilized eggs. Using either of two plasmid constructs, we injected uncut DNA into fertilized eggs at the one- or two-cell stage. Fish hatched from inject...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Culp P,Nüsslein-Volhard C,Hopkins N

更新日期：1991-09-15 00:00:00

abstract：:P/Q Ca(2+) channel activity is inhibited by G protein-coupled receptor activation. Channel inhibition requires a direct Gbetagamma binding onto the pore-forming subunit, Ca(v)2.1. It is characterized by biophysical changes, including current amplitude reduction, activation kinetic slowing, and an I-V curve shift, whic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sandoz G,Lopez-Gonzalez I,Grunwald D,Bichet D,Altafaj X,Weiss N,Ronjat M,Dupuis A,De Waard M

更新日期：2004-04-20 00:00:00

abstract：:Most humans in the United States have been infected with BK virus (BKV), a human papovavirus. Because BKV has oncogenic properties, we have investigated whether it may be a cause of human cancer. Basic principles of tumor virology imply that BKV-induced tumors should contain BKV DNA sequences. Therefore, we assayed (b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wold WS,Mackey JK,Brackmann KH,Takemori N,Rigden P,Green M

更新日期：1978-01-01 00:00:00

abstract：:Most of our knowledge about transmission at central synapses has been obtained by studying populations of synapses, but some important properties of synapses can be determined only by studying them individually. An important issue is whether a presynaptic action potential causes, at most, a single vesicle to be releas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conti R,Lisman J

更新日期：2003-04-15 00:00:00

abstract：:Amphibians are a bellwether for environmental degradation, even in natural ecosystems such as Yellowstone National Park in the western United States, where species have been actively protected longer than anywhere else on Earth. We document that recent climatic warming and resultant wetland desiccation are causing sev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McMenamin SK,Hadly EA,Wright CK

更新日期：2008-11-04 00:00:00

abstract：:Psoralens linked to triplex-forming oligonucleotides (psoTFOs) have been used in conjunction with laser-induced two-photon excitation (TPE) to damage a specific DNA target sequence. To demonstrate that TPE can initiate photochemistry resulting in psoralen-DNA photoadducts, target DNA sequences were incubated with psoT...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oh DH,King BA,Boxer SG,Hanawalt PC

更新日期：2001-09-25 00:00:00

abstract：:The prediction of binding sites and the understanding of interfaces associated with protein complexation remains an open problem in molecular biophysics. This work shows that a crucial factor in predicting and rationalizing protein-protein interfaces can be inferred by assessing the extent of intramolecular desolvatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernández A,Scheraga HA

更新日期：2003-01-07 00:00:00

abstract：:We investigated the participation of genomic regulatory events in the response of the songbird brain to a natural auditory stimulus of known physiological and behavioral relevance, birdsong. Using in situ hybridization, we detected a rapid increase in forebrain mRNA levels of an immediate-early gene encoding a transcr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mello CV,Vicario DS,Clayton DF

更新日期：1992-08-01 00:00:00

abstract：:In the prostate gland of adult mammals, most epithelial cells are in a state of proliferative quiescence. Androgens regulate this effect by inducing cell cycle arrest in the G(0)/G(1) phase. Potential mediators of this androgen-induced proliferative shutoff were identified by means of subtracted cDNA libraries. The ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geck P,Maffini MV,Szelei J,Sonnenschein C,Soto AM

更新日期：2000-08-29 00:00:00

abstract：:Active opioid binding proteins were solubilized from rat brain membranes in high yield with sodium deoxycholate in the presence of NaCl. Purification of opioid binding proteins was accomplished by opioid antagonist affinity chromatography. Chromatography using the delta-opioid antagonist N,N-diallyl-Tyr-D-Leu-Gly-Tyr-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loukas S,Mercouris M,Panetsos F,Zioudrou C

更新日期：1994-05-10 00:00:00

abstract：:The serological properties of the gag gene products p15 and p12 of N- and B-tropic viruses of C57BL mice have been examined. Although these viruses were serologically identical by competition assays for proteins gp71 and p30, they were readily distinguishable in competition assays for proteins p15 and p12. Two isolate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benade LE,Ihle JN,Declève A

更新日期：1978-09-01 00:00:00

abstract：:The emergence of Vibrio cholerae O139 Bengal during 1992-1993 was associated with large epidemics of cholera in India and Bangladesh and, initially, with a total displacement of the existing V. cholerae O1 strains. However, the O1 strains reemerged in 1994 and initiated a series of disappearance and reemergence of eit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Faruque SM,Sack DA,Sack RB,Colwell RR,Takeda Y,Nair GB

更新日期：2003-02-04 00:00:00

abstract：:The introduction of foreign genes into early mouse embryos and embryonic stem (ES) cells is invaluable for the analysis of gene function and regulation in the living animal. The use of vectors derived from retroviruses as gene transfer vehicles in this setting has had limited success because of silencing of transgene ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pfeifer A,Ikawa M,Dayn Y,Verma IM

更新日期：2002-02-19 00:00:00

abstract：:microRNAs comprise a few percent of animal genes and have been recognized as important regulators of a diverse range of biological processes. Understanding the biological functions of miRNAs requires effective means to identify their targets. Combined efforts from computational prediction, miRNA over-expression or dep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong X,Hammell M,Ambros V,Cohen SM

更新日期：2009-09-01 00:00:00

abstract：:GLQ223 is a highly purified, formulated preparation of trichosanthin, a 26-kDa plant-derived ribosome-inactivating protein with potent inhibitory activity against human immunodeficiency virus (HIV) in vitro. The compound produced concentration-dependent inhibition of HIV replication in acutely infected cultures of T-l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McGrath MS,Hwang KM,Caldwell SE,Gaston I,Luk KC,Wu P,Ng VL,Crowe S,Daniels J,Marsh J

更新日期：1989-04-01 00:00:00

abstract：:The coelacanth fish Latimeria chalumnae is the sole surviving species of a phylogenetic lineage that was founded more than 400 million years ago and that has changed morphologically very little since that time. Little is known about the molecular evolution of this "living fossil," considered by some taxonomists to be ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Betz UA,Mayer WE,Klein J

更新日期：1994-11-08 00:00:00

abstract：:Lifelong interactions between host and the ubiquitous and persistent cytomegalovirus (CMV) have been proposed to contribute to the age-related decline in immunity. Prior work from us and others found some support for that idea, yet evidence that this led to increased vulnerability to other infections was not obtained....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smithey MJ,Venturi V,Davenport MP,Buntzman AS,Vincent BG,Frelinger JA,Nikolich-Žugich J

更新日期：2018-07-17 00:00:00

abstract：:Nanoparticles are increasingly used in various fields, including biomedicine and electronics. One application utilizes the opacifying effect of nano-TiO(2), which is frequently used as pigment in cosmetics. Although TiO(2) is believed to be biologically inert, an emerging literature reports increased incidence of resp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yazdi AS,Guarda G,Riteau N,Drexler SK,Tardivel A,Couillin I,Tschopp J

更新日期：2010-11-09 00:00:00

abstract：:Administration of epidermal growth factor (EGF) to neonatal mice resulted in rapid tyrosine phosphorylation of a number of specific substrates in liver, kidney, lung, bladder, skin, and brain as detected by Western blot analysis of tissue homogenates with anti-phosphotyrosine antibodies. In the liver, three prominent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donaldson RW,Cohen S

更新日期：1992-09-15 00:00:00

abstract：:The role of protein kinase C (PKC) as a mediator of glucose-induced insulin secretion has been a subject of controversy. Glucose-induced translocation of PKC has not been reported, and the relevant PKC isoenzymes in islets have not been identified. To address these issues, we developed specific antibodies to the alpha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ganesan S,Calle R,Zawalich K,Smallwood JI,Zawalich WS,Rasmussen H

更新日期：1990-12-01 00:00:00

abstract：:We have asked here how the remarkable variation in maize haplotype structure affects recombination. We compared recombination across a genetic interval of 9S in 2 highly dissimilar heterozygotes that shared 1 parent. The genetic interval in the common haplotype is approximately 100 kb long and contains 6 genes intersp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He L,Dooner HK

更新日期：2009-05-26 00:00:00

abstract：:When the human cDNA, isolated on the basis of homology to the murine carbonic anhydrase (CA) "Y" was expressed in COS cells, the human CA was targeted to and processed in mitochondria, as expected for CA-V. However, tissue distribution reported for the corresponding mouse CA Y mRNA was much more limited than that repo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagao Y,Srinivasan M,Platero JS,Svendrowski M,Waheed A,Sly WS

更新日期：1994-10-25 00:00:00

abstract：:Thymidine dinucleotide (pTpT) stimulates melanogenesis in mammalian pigment cells and intact skin, mimicking the effects of UV irradiation and UV-mimetic DNA damage. Here it is shown that, in addition to tanning, pTpT induces a second photoprotective response, enhanced repair of UV-induced DNA damage. This enhanced re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eller MS,Maeda T,Magnoni C,Atwal D,Gilchrest BA

更新日期：1997-11-11 00:00:00

abstract：:Terrestrial solar UVB radiation ( approximately 295-320 nm) readily induces cyclobutane pyrimidine dimers (CPDs) in human skin DNA that result in characteristic mutations associated with nonmelanoma skin cancer. The proinflammatory cytokine TNFalpha is important in mouse skin chemical carcinogenesis and is thought to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walker SL,Young AR

更新日期：2007-11-27 00:00:00

abstract：:Intracellular proteolytic processing of precursor polypeptides is an essential step in the maturation of many proteins, including plasma proteins, hormones, neuropeptides, and growth factors. Most frequently, propeptide cleavage occurs after paired basic amino acid residues. To date, no mammalian propeptide processing...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wise RJ,Barr PJ,Wong PA,Kiefer MC,Brake AJ,Kaufman RJ

更新日期：1990-12-01 00:00:00

abstract：:The shape of the presynaptic action potential (AP) has a strong impact on neurotransmitter release. Because of the small size of most terminals in the central nervous system, little is known about the regulation of their AP shape during natural firing patterns in vivo. The calyx of Held is a giant axosomatic terminal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sierksma MC,Borst JGG

更新日期：2017-04-18 00:00:00

abstract：:Argonaute and Piwi proteins are key players in the RNA silencing pathway, with the former interacting with micro-RNAs (miRNAs) and siRNAs, whereas the latter targets piwi-interacting RNAs (piRNAs) that are 2'-O-methylated (2(')-OCH(3)) at their 3' ends. Germline-specific piRNAs and Piwi proteins play a critical role i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tian Y,Simanshu DK,Ma JB,Patel DJ

更新日期：2011-01-18 00:00:00

abstract：:Two chlorophyll-protein complexes associated with photosystem II (PSII) of the cyanobacterium Anacystis nidulans R2 have been detected. The larger of the two complexes, CPVI-1, contained a 71-kDa and a 42-kDa protein. The 71-kDa protein was determined to be the anchor protein of the phycobilisomes (the light-harvestin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pakrasi HB,Riethman HC,Sherman LA

更新日期：1985-10-01 00:00:00

abstract：:Prothoracicotropic hormone (PTTH) is the central cerebral neurohormone in insect development. Its release has been believed for decades to be confined to one (or two) critical moments early in each developmental stage at which time it triggers prolonged activation of the prothoracic glands to synthesize and release th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vafopoulou X,Steel CG

更新日期：1996-04-16 00:00:00