Possible involvement of human D minigenes in the first complementarity-determining region of kappa light chains.

abstract：:A sensitive target binding assay has recently been shown to detect natural killer (NK) cells in the mouse. Preincubation of NK cells with detergent-solubilized cell-surface proteins of YAC lymphoma cells prevented subsequent binding to intact YAC targets. The NK target structures (NK-TS) consisted of three molecular s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roder JC,Rosén A,Fenyö EM,Troy FA

更新日期：1979-03-01 00:00:00

abstract：:The embryonic sites in which progenitors of the hematopoietic lineages first emerge are ideal regions to characterize both the cells and environment needed to initiate blood cell development. For a number of years both the murine yolk sac and embryo have been recognized to contain progenitors of B lymphocytes. However...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cumano A,Furlonger C,Paige CJ

更新日期：1993-07-15 00:00:00

abstract：:A complete experimental format is given for the reconstitution of human hemoglobin from the separated heme-free alpha- and beta-globin chains (alpha degrees, beta degrees) and hemin, by two alternative routes. Based on their oxygen binding properties, the reaction of the ferri-forms with reducing agent, and the respon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yip YK,Waks M,Beychok S

更新日期：1977-01-01 00:00:00

abstract：:A form of autosomal dominant juvenile myoclonic epilepsy is caused by a nonconservative missense mutation, A322D, in the GABAA receptor alpha1 subunit M3 transmembrane helix. We reported previously that the A322D mutation reduced total and surface alpha1(A322D) subunit protein and that residual alpha1(A322D) subunit r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gallagher MJ,Ding L,Maheshwari A,Macdonald RL

更新日期：2007-08-07 00:00:00

abstract：:We determined the nucleotide sequence of about 1 kilobase of DNA 3' to the 5' long terminal repeat of three noninfectious ad one infectious proviral DNA clones of spleen necrosis virus, an avian retrovirus, to determine if the types of nucleic acid changes involved in retrovirus mutation shed light on special features...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Rear JJ,Temin HM

更新日期：1982-02-01 00:00:00

abstract：:Lactobacillus acidophilus NCFM is a probiotic bacterium that has been produced commercially since 1972. The complete genome is 1,993,564 nt and devoid of plasmids. The average GC content is 34.71% with 1,864 predicted ORFs, of which 72.5% were functionally classified. Nine phage-related integrases were predicted, but ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Altermann E,Russell WM,Azcarate-Peril MA,Barrangou R,Buck BL,McAuliffe O,Souther N,Dobson A,Duong T,Callanan M,Lick S,Hamrick A,Cano R,Klaenhammer TR

更新日期：2005-03-15 00:00:00

abstract：:The primary structure of the precursor of urotensin I, a neuropeptide hormone from the caudal neurosecretory system of the carp Cyprinus carpio, has been determined by analyzing the nucleotide sequence of cloned DNA complementary to the mRNA encoding it. The precursor consists of 145 amino acid residues and the carbox...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishida I,Ichikawa T,Deguchi T

更新日期：1986-01-01 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDAC) cells (PCC) have an exceptional propensity to metastasize early into intratumoral, chemokine-secreting nerves. However, we hypothesized the opposite process, that precancerous pancreatic cells secrete chemokines that chemoattract Schwann cells (SC) of nerves and thus induce read...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Demir IE,Kujundzic K,Pfitzinger PL,Saricaoglu ÖC,Teller S,Kehl T,Reyes CM,Ertl LS,Miao Z,Schall TJ,Tieftrunk E,Haller B,Diakopoulos KN,Kurkowski MU,Lesina M,Krüger A,Algül H,Friess H,Ceyhan GO

更新日期：2017-01-03 00:00:00

abstract：:The Ah receptor (AHR) is a basic helix-loop-helix protein that mediates the effects of 2,3,7,8-tetrachloro-dibenzo-p-dioxin. In this report, we describe a rabbit reticulocyte system that allows functional expression of both the AHR and its dimeric partner, the AHR nuclear translocator protein (ARNT). By using this in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dolwick KM,Swanson HI,Bradfield CA

更新日期：1993-09-15 00:00:00

abstract：:The emerging picture of Earth's deep interior from seismic tomography indicates more complexity than previously thought. The presence of lateral anisotropy and heterogeneity in Earth's mantle highlights the need for fully anisotropic elasticity data from mineral physics. A breakthrough in high-frequency (gigahertz) ul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacobsen SD,Spetzler H,Reichmann HJ,Smyth JR

更新日期：2004-04-20 00:00:00

abstract：:Eleven amino acid positions in the helix-turn-helix of lambda repressor have been mutagenized by using a combinatorial method in which alanine is substituted at each position with a probability of 0.5. Approximately 25% of the 2048 proteins in the resulting binomial library are active, including some variants with as ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gregoret LM,Sauer RT

更新日期：1993-05-01 00:00:00

abstract：:Transgenic mice expressing the sequences coding for the envelope proteins of the hepatitis B virus (HBV) in the liver have been used as a model of the HBV chronic carrier state. We evaluated the possibility of inducing a specific immune response to the viral envelope antigens and thus potentially controlling chronic H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mancini M,Hadchouel M,Davis HL,Whalen RG,Tiollais P,Michel ML

更新日期：1996-10-29 00:00:00

abstract：:In rod photoreceptors, several phototransduction components display light-dependent translocation between cellular compartments. Notably, the G protein transducin translocates from rod outer segments to inner segments/spherules in bright light, but the functional consequences of translocation remain unclear. We genera...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Majumder A,Pahlberg J,Boyd KK,Kerov V,Kolandaivelu S,Ramamurthy V,Sampath AP,Artemyev NO

更新日期：2013-07-23 00:00:00

abstract：:The radial distributions of the Calpha and side-chain atoms in a sample of 13 native proteins have been examined. It is found that there are substantial differences in behavior between different atoms of the same amino acid. In particular, the Calpha atoms of polar residues show no particular preference for being far ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rackovsky S,Scheraga HA

更新日期：1977-12-01 00:00:00

abstract：:Varicella-zoster virus (VZV) causes varicella and establishes latency in sensory ganglia. VZV reactivation results in herpes zoster. We developed a model using human dorsal root ganglion (DRG) xenografts in severe combined immunodeficient (SCID) mice to investigate VZV infection of differentiated neurons and satellite...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zerboni L,Ku CC,Jones CD,Zehnder JL,Arvin AM

更新日期：2005-05-03 00:00:00

abstract：:Global declines in biodiversity are altering disease dynamics in complex and multifaceted ways. Changes in biodiversity can have several outcomes on disease risk, including dilution and amplification effects, both of which can have a profound influence on the effects of disease in a community. The dilution effect occu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Searle CL,Biga LM,Spatafora JW,Blaustein AR

更新日期：2011-09-27 00:00:00

abstract：:Microtubules are polarized polymers that exhibit dynamic instability, with alternating phases of elongation and shortening, particularly at the more dynamic plus-end. Microtubule plus-end tracking proteins (+TIPs) localize to and track with growing microtubule plus-ends in the cell. +TIPs regulate microtubule dynamics...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dixit R,Barnett B,Lazarus JE,Tokito M,Goldman YE,Holzbaur EL

更新日期：2009-01-13 00:00:00

abstract：:New important applications of copper metal, e.g., in the areas of hydrogen production, fuel cell operation, and spent nuclear fuel disposal, require accurate knowledge of the physical and chemical properties of stable and metastable copper compounds. Among the copper(I) compounds with oxygen and hydrogen, cuprous oxid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Korzhavyi PA,Soroka IL,Isaev EI,Lilja C,Johansson B

更新日期：2012-01-17 00:00:00

abstract：:We present a mandible recovered in 2003 from the Aurora Stratum of the TD6 level of the Gran Dolina site (Sierra de Atapuerca, northern Spain). The specimen, catalogued as ATD6-96, adds to the hominin sample recovered from this site in 1994-1996, and assigned to Homo antecessor. ATD6-96 is the left half of a gracile m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carbonell E,Bermúdez de Castro JM,Arsuaga JL,Allue E,Bastir M,Benito A,Cáceres I,Canals T,Díez JC,van der Made J,Mosquera M,Ollé A,Pérez-González A,Rodríguez J,Rodríguez XP,Rosas A,Rosell J,Sala R,Vallverdú J,Vergés

更新日期：2005-04-19 00:00:00

abstract：:Photochemical release (uncaging) of bioactive messengers with three-dimensional spatial resolution in light-scattering media would be greatly facilitated if the photolysis could be powered by pairs of IR photons rather than the customary single UV photons. The quadratic dependence on light intensity would confine the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furuta T,Wang SS,Dantzker JL,Dore TM,Bybee WJ,Callaway EM,Denk W,Tsien RY

更新日期：1999-02-16 00:00:00

abstract：:A vaccinia virus host range mutant (hr mutant) deleted of 18 kilobase pairs at the left end of the genome has been employed to precisely map a viral gene required for multiplication in human cells. DNA fragments from the wild-type virus were inserted into the thymidine kinase gene of mutant virus by means of in vivo h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gillard S,Spehner D,Drillien R,Kirn A

更新日期：1986-08-01 00:00:00

abstract：:The operon model was proposed by Jacob and Monod in 1961 to explain the regulation of enzyme synthesis in bacteria. The model requires two genetic elements: a locus i which directs the synthesis of a repressor, and a locus called the operon which contains an operator gene and associated structural genes. The repressor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zubay G,Lederman M

更新日期：1969-02-01 00:00:00

abstract：:A class II (B) general transcription factor of 34 kDa has been purified from HeLa cells to apparent homogeneity. This factor appears to be transcription factor IIB (TFIIB), since it binds in vitro to template-committed preinitiation complexes formed between a template containing the TATA box/cap-site elements of the a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moncollin V,Fischer L,Cavallini B,Egly JM,Chambon P

更新日期：1992-01-01 00:00:00

abstract：:Intrinsic genetic instability of RNA viruses may lead to the accumulation of revertants during manufacture of live viral vaccines, requiring rigorous quality control to ensure vaccine safety. Each lot of oral poliovirus vaccine (OPV) is tested for neurovirulence in animals and also for the presence of neurovirulent re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neverov A,Chumakov K

更新日期：2010-11-16 00:00:00

abstract：:Transgenic (Tg) mouse lines that express chimeric mouse-human prion protein (PrP), designated MHu2M, are susceptible to prions from patients with sporadic Creutzfeldt-Jakob disease (sCJD). With the aim of decreasing the incubation time to fewer than 200 days, we constructed transgenes in which one or more of the nine ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Korth C,Kaneko K,Groth D,Heye N,Telling G,Mastrianni J,Parchi P,Gambetti P,Will R,Ironside J,Heinrich C,Tremblay P,DeArmond SJ,Prusiner SB

更新日期：2003-04-15 00:00:00

abstract：:The polymerization of individual RecA-DNA filaments, containing either single-stranded or double-stranded DNA, was followed in real time, and their mechanical properties were characterized with force-measuring laser tweezers. It was found that the stretch modulus of a filament is dominated by its (central) DNA compone...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hegner M,Smith SB,Bustamante C

更新日期：1999-08-31 00:00:00

abstract：:Associative memory of the mollusc Hermissenda crassicornis, previously correlated with changes of specific K+ currents, protein phosphorylation, and increased synthesis of mRNA and specific proteins, is here shown to be accompanied by macroscopic alteration in the structure of a single identified neuron, the medial ty...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alkon DL,Ikeno H,Dworkin J,McPhie DL,Olds JL,Lederhendler I,Matzel L,Schreurs BG,Kuzirian A,Collin C

更新日期：1990-02-01 00:00:00

abstract：:Infants born prematurely risk significant life-long cognitive disability, representing a major pediatric health crisis. The neuropathology of this cohort is accurately modeled in mice subjected to sublethal postnatal hypoxia. Massively parallel transcriptome analysis using cDNA microchips (9,262 genes), combined with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curristin SM,Cao A,Stewart WB,Zhang H,Madri JA,Morrow JS,Ment LR

更新日期：2002-11-26 00:00:00

abstract：:To generate antibodies with different effector functions, B cells undergo Immunoglobulin Heavy Chain (IgH) class switch recombination (CSR). The ligation step of CSR is usually mediated by the classical nonhomologous end-joining (cNHEJ) pathway. In cNHEJ-deficient cells, a remarkable ∼25% of CSR can be achieved by the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang XS,Zhao J,Wu-Baer F,Shao Z,Lee BJ,Cupo OM,Rabadan R,Gautier J,Baer R,Zha S

更新日期：2020-10-13 00:00:00

abstract：:The tumor suppressor activity of p53 is regulated by interactions with the ubiquitin ligase HDM2 and the general transcriptional coactivators CBP and p300. Using NMR spectroscopy and isothermal titration calorimetry, we have dissected the binding interactions between the N-terminal transactivation domain (TAD) of p53,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferreon JC,Lee CW,Arai M,Martinez-Yamout MA,Dyson HJ,Wright PE

更新日期：2009-04-21 00:00:00