Abstract:
:Basolateral, brush-border, and Golgi-enriched subcellular membrane fractions, prepared from homogenates of rat small intestinal mucosa obtained by scraping, had unusually high concentrations of nonesterified fatty acids. These fatty acids appear to be responsible for the large amount of calcium binding, an effect that previously was shown to be reduced in vitamin D deficiency. In contrast, basolateral and Golgi membranes prepared from isolated cells had low levels of nonesterified fatty acids and calcium binding. Intermediate levels were found with isolated cells that were not put through the usual washing procedures. Addition to homogenates of scrapings of a lipase inhibitor, diethyl-p-nitrophenyl phosphate, reduced calcium binding and nonesterified fatty acids to levels similar to those in membranes prepared from isolated cells. Phospholipase A activity was low in homogenates of isolated cells and high in scrapings; this was reduced in intestinal scrapings of vitamin D-deficient rats. Ileal membranes had more calcium binding than duodenal membranes, and ileal homogenates also had greater phospholipase A activity. Preparation of subcellular membranes from rat intestinal scrapings can result in altered lipid composition, probably due to lipolytic enzyme activity; in addition to increasing cation-binding, these high levels of fatty acids may affect other membrane properties and enzyme function.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Walters JR,Horvath PJ,Weiser MMdoi
10.1016/0016-5085(86)90435-xsubject
Has Abstractpub_date
1986-07-01 00:00:00pages
34-40issue
1eissn
0016-5085issn
1528-0012pii
0016-5085(86)90435-Xjournal_volume
91pub_type
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