Ectopic implantation studies using Sl/Sld marrow and recipients.

abstract：BACKGROUND:Autologous bone marrow transplantation is an important therapy for patients with acute myelogenous leukemia (AML). However, leukemia in the graft may contribute to posttransplant relapse. Treatment of the graft with 4-hydroperoxycyclophosphamide (4HC) is sometimes used to decrease numbers of infused leukemia...

journal_title：Experimental hematology

authors： Miller CB,Rowlings PA,Zhang MJ,Jones RJ,Piantadosi S,Keating A,Armitage JO,Calderwood S,Harris RE,Klein JP,Lazarus HM,Linker CA,Sobocinski KA,Weisdorf D,Horowitz MM

更新日期：2001-11-01 00:00:00

abstract：:The concept that mesenchymal stromal cells (MSCs), a component of the hematopoietic microenvironment, can be a target for alloreactive effector cells in the context of graft-vs-host disease has not been investigated in detail. Mixed lymphocyte reaction (MLR) supernatant was used to mimic the inflammatory milieu induce...

journal_title：Experimental hematology

authors： Fasslrinner F,Wobus M,Duryagina R,Müller K,Stopp S,Wehner R,Rauner M,Hofbauer LC,Schmitz M,Bornhäuser M

更新日期：2012-11-01 00:00:00

abstract：:An improved negative immunomagnetic selection strategy has been devised for the enrichment of primitive hemopoietic cells using the high proliferative potential colony-forming cell (HPP-CFC) assay as an index of stem cell purification. Immunomagnetic selection was carried out using goat anti-rat conjugated M-450 Dynab...

journal_title：Experimental hematology

authors： Bertoncello I,Bradley TR,Watt SM

更新日期：1991-02-01 00:00:00

abstract：OBJECTIVE:Adult T-cell leukemia (ATL) is a mature CD4(+) T-cell malignancy caused by infection with human T-lymphotrophic virus type-1 and is associated with a marked hypercalcemia in many patients. Recently, it has been proposed that macrophage inflammatory protein-1alpha (MIP-1alpha) is the clinical hallmark of hyper...

journal_title：Experimental hematology

authors： Matsumoto K,Murao K,Imachi H,Nishiuchi T,Cao W,Yu X,Li J,Ahmed RA,Iwama H,Kobayashi R,Tokumitsu H,Ishida T

更新日期：2008-04-01 00:00:00

abstract：:Serum erythropoietin (Epo) levels are depressed in anemic AIDS patients relative to controls. The basis for abnormal regulation of Epo has not been defined. The hepatoma cell line Hep3B produces Epo in response to hypoxia and serves as a model for the study of Epo regulation. Hep3B cells are infectible with human immu...

journal_title：Experimental hematology

authors： Wang Z,Goldberg MA,Scadden DT

更新日期：1993-05-01 00:00:00

abstract：OBJECTIVE:Regulation of the cell cycle by cyclin-dependent kinase (CDK) activity occurs at multiple levels and is often altered in human cancers. Therefore, CDK activity has been targeted for drug discovery, and a number of small molecules have now been identified as CDK inhibitors. Plant cytokinin analogues with CDK i...

journal_title：Experimental hematology

authors： Vermeulen K,Strnad M,Havlícek L,Van Onckelen H,Lenjou M,Nijs G,Van Bockstaele DR,Berneman ZN

更新日期：2002-10-01 00:00:00

abstract：:Megakaryocytic differentiation of progenitor cells was investigated in nine patients with low-risk myelodysplastic syndromes (MDS) (eight refractor anemia [RA] and one RA with ringed sideroblasts [RARS] and five patients with high-risk MDS (two RA with excess of blasts [RAEB] and three RAEB in transformation [RAEB-T])...

journal_title：Experimental hematology

authors： Hofmann WK,Kalina U,Wagner S,Seipelt G,Ries C,Hoelzer D,Ottmann OG

更新日期：1999-03-01 00:00:00

abstract：OBJECTIVE:Our previous studies have demonstrated differentially expressed genetic signature patterns in adult peripheral blood (APB) monocytes (Mos) vs cord blood (CB) Mos. MATERIALS AND METHODS:In this study, we examined the differential gene expression profiles of APB vs CB immature dendritic cells (iDCs) and mature...

journal_title：Experimental hematology

authors： Jiang H,van de Ven C,Baxi L,Satwani P,Cairo MS

更新日期：2009-10-01 00:00:00

abstract：:The results and complications of 224 marrow collections from 200 patients with malignant disease who underwent marrow aspiration and storage for subsequent autologous marrow transplantation (AMT) were analyzed. The median age of the patients was 35 years (range 1-68); 131 patients had hematologic malignancies and 69 h...

journal_title：Experimental hematology

authors： Jin NR,Hill RS,Petersen FB,Buckner CD,Stewart PS,Amos D,Appelbaum FR,Clift RA,Bensinger WI,Sanders JE

更新日期：1985-10-01 00:00:00

abstract：OBJECTIVE:Activating mutations in FLT3 are known to be a frequent transforming event in acute myeloid leukemia. Small molecule-inhibitor therapy targeting the FLT3 kinase is, therefore, an attractive strategy. FLT3 kinase inhibitors, such as PKC412, have already entered clinical trials. Even though results are encourag...

journal_title：Experimental hematology

authors： Kancha RK,Grundler R,Peschel C,Duyster J

更新日期：2007-10-01 00:00:00

abstract：:A transplanted tumor that induces granulocytosis and produces colony stimulating factor (CSF) was studied in mice during several passages. The sequence of events leading to granulocytosis was characterized. Band granulocytes were increased 3 days after tumor inoculation, while simultaneously CFU-s and CFU-c in bone ma...

journal_title：Experimental hematology

authors： Reincke U,Burlington H,Carsten AL,Cronkite EP,Laissue JA

更新日期：1978-04-01 00:00:00

abstract：OBJECTIVE:The purpose of this study was to evaluate the repopulating properties of bone marrow (BM) from mice irradiated during embryonic and adult stages of development. MATERIALS AND METHODS:Four-day-old embryos, 17-day-old fetuses, and 12-week-old mice were irradiated with of 1 or 3 Gy of x-rays. At 3 and 9 months ...

journal_title：Experimental hematology

authors： Grande T,Varas F,Bueren JA

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:In patients treated with rituximab and alemtuzumab for lymphomas or CLL, antibody-dependent cellular cytotoxicity (ADCC) is a major mechanism of action. Therefore, assessment of ADCC is mandatory to understand the complex mechanisms leading to the anti-lymphoma effects of monoclonal antibodies (mAb). Due to ...

journal_title：Experimental hematology

authors： Fischer L,Penack O,Gentilini C,Nogai A,Muessig A,Thiel E,Uharek L

更新日期：2006-06-01 00:00:00

abstract：:A peptide sequence was identified by phage display technology that could be used as an alternative to chymopapain for the release of hematopoietic progenitor cells captured by anti-CD34 monoclonal antibodies. This was achieved by affinity selection screening (biopanning) of a random hexapeptide sequence phage display ...

journal_title：Experimental hematology

authors： Tseng-Law J,Szalay P,Guillermo R,Kobori J,Van Epps D,Schneidkraut MJ,Deans R

更新日期：1999-05-01 00:00:00

abstract：:The rae28 gene (rae28) is a murine homologue of the Drosophila polyhomeotic gene, which is a member of the Polycomb-group genes. In this study, we examined the role of rae28 in lymphocyte development. Because homozygous rae28-deficient (rae28-/-) mice died in the perinatal period, we examined lymphocyte development by...

journal_title：Experimental hematology

authors： Tokimasa S,Ohta H,Sawada A,Matsuda Y,Kim JY,Nishiguchi S,Hara J,Takihara Y

更新日期：2001-01-01 00:00:00

abstract：OBJECTIVE:Donor T cells expressing lymph node homing receptors are the foremost initiators of acute graft-vs-host disease (aGVHD), and a high proportion of CD4(+)CCR7(+) T cells in human leukocyte antigen-matched allografts has been shown to confer a high risk of aGVHD without interfering in other outcomes. METHODS:Na...

journal_title：Experimental hematology

authors： Yakoub-Agha I,Saule P,Depil S,Grutzmacher C,Boulanger F,Magro L,Jouet JP,Dessaint JP,Labalette M

更新日期：2007-06-01 00:00:00

abstract：:Interferon α (IFNα) is used clinically to restore polyclonal hematopoiesis in patients with the myeloproliferative neoplasms polycythemia vera and essential thrombocythemia and to improve chemosensitivity in chronic myeloid leukemia patients. However, the mechanisms by which IFNα affects disease-initiating hematopoiet...

journal_title：Experimental hematology

authors： King KY,Matatall KA,Shen CC,Goodell MA,Swierczek SI,Prchal JT

更新日期：2015-10-01 00:00:00

abstract：:Discontinuous albumin density gradients were used to physically separate hemopoietic cells from immunocompetent lymphocytes in peripheral blood mononuclear cells of dogs. Transplantation of these stem cell concentrates into lethally irradiated allogeneic recipients restored hemopoiesis in nine of 12 animals. Tolerance...

journal_title：Experimental hematology

authors： Gerhartz HH,Nothdurft W,Carbonell F,Fliedner TM

更新日期：1985-02-01 00:00:00

abstract：OBJECTIVE:The purpose of this study was to investigate the effects of interleukin-2 (IL-2) gene-transduced hematopoietic progenitor cells or cytotoxic function and systemic toxicity following syngeneic bone marrow transplantation. MATERIAL AND METHODS:Marrow of 5-fluorouracil pretreated donor mice were transfected wit...

journal_title：Experimental hematology

authors： Kühr T,Bechter O,Dirnhofer S,Geley S,Gächter A,Pall G,Url M,Dietrich H,Oberaigner P,Klima G,Eisterer W,Hilbe W,Lukas P,Thaler J

更新日期：2000-08-01 00:00:00

abstract：:We have developed a prospective approach to identify the generation of erythroid cells derived from cultured peripheral blood mononuclear cells (PBMC) by monitoring the expression of the cell surface protein CD48. Unpurified populations of PBMC obtained from the buffy coats of normal volunteers were grown in suspensio...

journal_title：Experimental hematology

authors： Miller JL,Njoroge JM,Gubin AN,Rodgers GP

更新日期：1999-04-01 00:00:00

abstract：:The present study was undertaken to determine whether neutrophilic granulocytes leave the vascular bed and enter body tissues, particularly the lung, in pathogen-free mice under normal steady state conditions and also under inflammatory stress. Tissues from normal mice and from mice with granulocytosis-producing tumor...

journal_title：Experimental hematology

authors： Jamuar MP,Cronkite EP

更新日期：1980-08-01 00:00:00

abstract：:Previous systems for cloning human T-cells have been either one step procedures with small colony size (40 less than 40 cells) or two-step procedures with agglutination problems. These systems also require erythrocytes, thiols and human serum. We have regularly grown more than 10(3) colonies (more than 40 cells)/5 X 1...

journal_title：Experimental hematology

authors： Spitzer G,Verma DS,Zander A,Beran M,Dicke KA,Siegel S

更新日期：1980-11-01 00:00:00

abstract：:The circadian and circannual rhythms of committed stem cells (CFUc) were investigated by using the agar colony technique. There was a marked circadian rhythmicity, with variations between maximum and minimum colony yields of 2-5.5 times; such variations could also be seen throughout the year. A phase shift was observe...

journal_title：Experimental hematology

authors： Aardal NP

更新日期：1984-01-01 00:00:00

abstract：:In chronic eosinophilic leukemia, the transforming oncoprotein FIP1L1-PDGFRA is a major target of therapy. In most patients, the tyrosine kinase inhibitor (TKI) imatinib induces complete remission. For patients who are intolerant or resistant, novel TKIs have been proposed. We examined the in vitro effects of 14 kinas...

journal_title：Experimental hematology

authors： Sadovnik I,Lierman E,Peter B,Herrmann H,Suppan V,Stefanzl G,Haas O,Lion T,Pickl W,Cools J,Vandenberghe P,Valent P

更新日期：2014-04-01 00:00:00

abstract：OBJECTIVE:To investigate the interaction of imatinib mesylate (IM) with the clinically relevant adenosine triphosphate-binding cassette efflux transporter MDR1 (ABCB1) in cells from patients with chronic myeloid leukemia (CML) and to explore whether inhibition of this transporter would improve IM's efficacy in the elim...

journal_title：Experimental hematology

authors： Hatziieremia S,Jordanides NE,Holyoake TL,Mountford JC,Jørgensen HG

更新日期：2009-06-01 00:00:00

abstract：:Protein kinase activities are involved in cellular proliferation and differentiation, and inhibitors of these activities are useful for studying the mechanisms of induction of differentiation. We found that staurosporine, an inhibitor of protein kinase activities, induced morphological differentiation of human myelobl...

journal_title：Experimental hematology

authors： Makishima M,Honma Y,Hozumi M,Sampi K,Motoyoshi K,Nagata N,Hattori M

更新日期：1993-07-01 00:00:00

abstract：:Ouabain has been shown to increase the number of clonally derived erythroid stem cells, CFUE and BFUE, from normal murine bone marrow. We report here that digoxin and theophylline also enhance erythroid stem cell colony formation, in the presence of suboptimal concentrations of erythropoietin (Ep) (0.01 IU/ml) in meth...

journal_title：Experimental hematology

authors： Gallicchio VS,Chen MG,Murphy MJ Jr

更新日期：1982-09-01 00:00:00

abstract：:We compared the effects of recombinant (r) tumor necrosis factor (TNF) and lymphotoxin (rLT) on in vitro colony formation by erythroid, multipotential and granulocyte, monocyte (CFU-GM) precursor cells. Recombinant granulocyte, macrophage-colony stimulating factor and bone marrow preparations enriched for hematopoieti...

journal_title：Experimental hematology

authors： Murphy M,Perussia B,Trinchieri G

更新日期：1988-02-01 00:00:00

abstract：:Erythropoietin (Epo), a glycoprotein hormone, regulates the proliferation and differentiation of committed erythroid progenitor cells. We investigated the effect of Epo on the kinetics of an Epo-dependent cell line (DA-1ER), which was cloned from the murine interleukin 3-dependent cell line, DA-1. Flow cytometry and [...

journal_title：Experimental hematology

authors： Tsuda H,Sawada T,Kawakita M,Takatsuki K

更新日期：1989-03-01 00:00:00

abstract：:Anamorsin (AM) is an antiapoptotic molecule that confers factor-independent survival on hematopoietic cells. AM-deficient (AM(-/-)) mice are embryonic lethal because of a defect in definitive hematopoiesis; however, the significance of AM in embryonic hematopoiesis remains unknown. This study characterized the hematop...

journal_title：Experimental hematology

authors： Tanimura A,Shibayama H,Hamanaka Y,Fujita N,Ishibashi T,Sudo T,Yokota T,Ezoe S,Tanaka H,Matsumura I,Oritani K,Kanakura Y

更新日期：2014-05-01 00:00:00