Abstract：
OBJECTIVE:Our previous studies have demonstrated differentially expressed genetic signature patterns in adult peripheral blood (APB) monocytes (Mos) vs cord blood (CB) Mos. MATERIALS AND METHODS:In this study, we examined the differential gene expression profiles of APB vs CB immature dendritic cells (iDCs) and mature dendritic cells (mDCs) derived from Mos. RESULTS:By utilizing oligonucleotide microarray, significant differential gene expression signature patterns were identified in APB vs CB mDCs, including increased expression of chemokines, cytokine receptors, and cell surface molecules. Additionally, signaling protein genes were significantly overexpressed in APB vs CB mDCs. There was also a significant amplification of expression of transcription factor interferon (IFN) regulatory factors and structure regulatory genes in APB vs CB mDCs. In contrast, there were genes expressed significantly higher in CB vs APB mDCs, including cell-cycle regulators and signaling molecule gene. CONCLUSION:Taken together, these results suggest that specific genetic signatures might be responsible for differential DC differentiation and maturation between APB vs CB, and may provide insight into molecular mechanisms regulating differential immune responses between neonates and adults.
journal_name
Exp Hematoljournal_title
Experimental hematologyauthors
Jiang H,van de Ven C,Baxi L,Satwani P,Cairo MSdoi
10.1016/j.exphem.2009.07.010subject
Has Abstractpub_date
2009-10-01 00:00:00pages
1201-15issue
10eissn
0301-472Xissn
1873-2399pii
S0301-472X(09)00310-5journal_volume
37pub_type
杂志文章abstract：:Dendritic cells (DC) are recognized as the most potent antigen-presenting cells with the ability to stimulate naive resting T cells and to initiate primary immune responses. Encouraging results in vaccination studies in animal models and the development of protocols to generate sufficient numbers of human DC for clini...
journal_title：Experimental hematology
pub_type： 杂志文章,评审
doi：10.1016/s0301-472x(01)00730-5
更新日期：2001-11-01 00:00:00
abstract：:Interferon (INF)-α was the maintenance treatment of choice after autologous stem cell transplantation in multiple myeloma in the past, but currently Thalidomide is commonly used. In this prospective study, the implications of the various types of maintenance therapy on the patients T cell pattern and activation status...
journal_title：Experimental hematology
pub_type： 临床试验,杂志文章
doi：10.1016/j.exphem.2012.10.018
更新日期：2013-03-01 00:00:00
abstract：:Induced pluripotent stem cells (iPS) derived from disease cells are expected to provide a new experimental material, especially for diseases from which samples are difficult to obtain. In this study, we generated iPS from samples from patients with primary and secondary myelofibrosis. The primary myelofibrosis cells h...
journal_title：Experimental hematology
pub_type： 临床试验,杂志文章
doi：10.1016/j.exphem.2014.03.010
更新日期：2014-09-01 00:00:00
abstract：:Embryonic and adult stem cell populations have great potential value in medicine, and hematopoietic stem cells are already being used in transplantation. Definition of these populations to increase our understanding of the programs that control differentiation, self-renewal, and possibly plasticity would be of great i...
journal_title：Experimental hematology
pub_type： 杂志文章,评审
doi：10.1016/j.exphem.2003.08.012
更新日期：2003-12-01 00:00:00
abstract：:In vitro studies of human hemopoiesis are often limited by the availability of normal bone marrow. We have overcome this difficulty by taking advantage of the bone marrow fragments removed during total hip replacement. We report here a comparative study of the colony forming capacity of trochanter and sternal or iliac...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1979-07-01 00:00:00
abstract：OBJECTIVE:Recruitment of endothelial progenitor cells to the sites of ischemia has recently been suggested as a mechanism of tissue repair. Here we address the hypothesis that the hypoxia-inducible full-length endothelial-monocyte-activating polypeptide II (EMAP II) provides a mechanism to recruit late outgrowth highly...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2006.05.021
更新日期：2006-08-01 00:00:00
abstract：:BCR-ABL-negative myeloproliferative neoplasms (MPNs) are driven by JAK-STAT pathway activation, but epigenetic alterations also play an important pathophysiological role. These can be pharmacologically manipulated with histone deacetylase inhibitors (HDACIs), which have proven to be clinically effective in the treatme...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2019.02.002
更新日期：2019-04-01 00:00:00
abstract：:The switch/sugar nonfermenting (SWI/SNF) family of chromatin remodeling complexes have been implicated in normal hematopoiesis. The ARID2 protein is a component of the polybromo-associated BAF (PBAF), one of the two main SWI/SNF complexes. In the current study, we used a conditional Arid2 knockout mouse model to deter...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2020.12.004
更新日期：2020-12-17 00:00:00
abstract：:In vitro treatment of parental C57BL/6 lymphohematopoietic cell grafts with unabsorbed guinea pig anti-mouse thymocyte serum (ATS) and guinea pig complement (GPC), prior to inoculation into lethally irradiated B6D2F hybrid hosts, has proven to be of value in terms of mitigating graft-versus-host disease (GvHD). Howeve...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:A modification of the fluorescence-activated cell sorter (FACS) was used to rapidly and reliably study the clonal proliferation of single hemopoietic cells. Murine FDC-P1 and human cord blood progenitor cells were examined for their ability to proliferate from single cells in 96-well microtiter plates containing agar ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:In a previous report, we described a method to generate cell lines derived from stromal colonies (colony-derived cell lines [CDCL]). In a first step, colonies were obtained from plating cells from adherent layers of human long-term bone marrow cultures (LTBMC) in methyl-cellulose in the presence of interleukin-1 beta ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：:The effect of recombinant interleukin 3 (IL-3) on the function of murine resident peritoneal macrophages was investigated. IL-3 enhanced the phagocytosis of Candida pseudotropicalis and Candida albicans and enhanced killing of the former. The enhanced killing is inhibited by scavengers of oxygen radicals, suggesting t...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：OBJECTIVES:To identify the regulatory sequences driving Gata1 expression in conventional dendritic cells (cDC). MATERIALS AND METHODS:The number and expression levels of Gata1, Gata1-target genes and hypersensitive site (HS) 2 (the eosinophil-specific enhancer)-driven green fluorescent protein (GFP) reporter of cDCs f...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2010.03.006
更新日期：2010-06-01 00:00:00
abstract：:The history of clonal hematopoiesis (CH) research is punctuated by several seminal discoveries that have forged our understanding of cancer development. The clever application of the principle of random X-chromosome inactivation (XCI) in females led to the development of the first test to identify clonal derivation of...
journal_title：Experimental hematology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.exphem.2020.01.008
更新日期：2020-03-01 00:00:00
abstract：:The immunostimulant poly(A)-poly(U) induces a rapid enhancement of circulating colony-stimulating activity (CSA) in normal mice, culminating 2 h after i.v. injection. A dose of 200 micrograms per mouse is sufficient for a maximal effect. The colonies formed in response to sera from poly(A)-poly(U)-injected mice are ma...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:A novel polypeptide with a molecular weight of 17 kd (17k protein) was identified in bone marrow cell cultures. The synthesis of 17k protein is elevated in cell cultures maintained under Dexter conditions, which support myelopoiesis. The predominance of macrophages in the stromal layer of these cultures and the observ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:Chloramphenicol, an antibiotic associated with reversible bone marrow suppression and fatal aplastic anemia, was found to increase human bone marrow granulocyte-macrophage colonies (CFU-GM) in vitro. Maximal stimulation was at a concentration of 1.0 micrograms/ml (3.1 microM), with inhibition at concentrations greater...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：OBJECTIVE:Progenipoietin-1 is an agonist of both the granulocyte colony-stimulating factor and fetal liver tyrosine kinase-3 receptors capable of inducing the proliferation of multiple hematopoietic cell lineages. The potential of progenipoietin-1 to mobilize transplantable hematopoietic stem cells into the peripheral ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(01)00675-0
更新日期：2001-08-01 00:00:00
abstract：OBJECTIVE:Umbilical cord blood (CB) provides an alternative source of hematopoietic progenitor cells for transplantation; however, prolonged thrombocytopenia remains a major obstacle due to the low numbers of megakaryocyte progenitor (Mk-prog) cells and their subsequent delayed engraftment. In this study, we improved t...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(02)00884-6
更新日期：2002-09-01 00:00:00
abstract：:Merocyanine 540 (MC 540) is a photosensitizing dye with antineoplastic and antiviral properties. Because photoexcited MC 540 generates singlet molecular oxygen and possibly other reactive oxygen species there is concern that MC 540-mediated photosensitization may be mutagenic. In this paper, we report on the induction...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：OBJECTIVE:To assess the contribution of bone marrow (BM)-derived endothelial progenitor cells (EPCs) to the neovascularisation of cutaneous incisional wounds. METHODS:Lethally irradiated C57Bl/6 mice were transplanted with BM mononuclear cells from Tie2/lacZ mice, which constitutively overexpressed beta-galactosidase ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2006.10.016
更新日期：2007-03-01 00:00:00
abstract：:Different human hematopoietic cell lines were analyzed for the presence of interleukin 6 (IL-6) and IL-6 receptor (IL-6-R). Both IL-6 mRNA and secreted active IL-6 protein were detectable in untreated cell lines with erythroid or megakaryoblastic features (K562, HEL, KU 812, MEG-01, and Dami), but they were not expres...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:Several transcription factors determine the cell fate decision between granulocytes and monocytes, but the upstream signal transduction pathways that govern myelopoiesis are largely unknown. Based on our observation of aberrant myeloid cell representation in hematopoietic tissues of 12/15-lipoxygenase (12/15-...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2010.07.004
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to evaluate the effect of CF101, a synthetic agonist to the A3 adenosine receptor (A3AR), on the production of granulocyte colony-stimulating factor (G-CSF). The ability of CF101 to act as a myeloprotective agent in chemotherapy-treated mice was tested. METHODS:CF101 was administere...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(02)00962-1
更新日期：2002-12-01 00:00:00
abstract：:We have previously shown that CD33- CD34+ human marrow cells are capable of giving rise to colony-forming cells (CFC) in long-term marrow culture (LTMC) but are mainly depleted of progenitors that directly form colonies in semi-solid media. In contrast, the CD33+ CD34+ population contains most of the CFC but not the p...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:To investigate the effect of interferon on the proliferation of normal erythroid precursor cells, various interferon preparations were added to mouse fetal liver cells cultured in a liquid microculture system. The effect of interferon was studied using cellular tritiated thymidine incorporation both in the presence an...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
abstract：OBJECTIVE:Monocyte-derived dendritic cells (DCs) play important roles in the immune response against infections and malignancies. Human herpesvirus 6 (HHV-6) infects monocytes and is reactivated in immunodeficient patients. To clarify the mechanisms of HHV-6-induced immunodeficiency, we investigated the effect of HHV-6...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2006.02.001
更新日期：2006-05-01 00:00:00
abstract：:Erythropoiesis requires large amounts of iron for hemoglobin synthesis. There are two sources of iron for erythropoiesis, dietary and stored iron; however, their relative contributions to erythropoiesis remain unknown. In this study, we used the stable iron isotope (57)Fe to quantify synthesis of hemoglobin derived fr...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2016.02.005
更新日期：2016-06-01 00:00:00
abstract：:Hematopoietic progenitor cells (low density, lineage negative [Lin-], depleted of CD4+, CD8+, B220+, Gr-1+, Mac-1+, CD71+, and Thy1.2+ cells) can engraft lethally irradiated mice when transplanted within, but not across the major histocompatibility (MHC) antigen barrier. We hypothesized that our progenitor cell prepar...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:The mechanism of accelerated erythropoiesis under the hypoxic conditions of high altitude (HA) remains largely obscure. Here, we investigated the potential role of bone marrow (BM) T cells in the increased production of erythrocytes at HA. We found that mice exposed to a simulated altitude of 6,000 m for 1-3 weeks exh...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2014.04.007
更新日期：2014-09-01 00:00:00
