当前位置： SCI文献检索 > EXPERIMENTAL HEMATOLOGY期刊下所有文献 > Comparative long-term effects of interferon α and hydroxyurea on human hematopoietic progenitor cells.

Comparative long-term effects of interferon α and hydroxyurea on human hematopoietic progenitor cells.

Abstract：

:Interferon α (IFNα) is used clinically to restore polyclonal hematopoiesis in patients with the myeloproliferative neoplasms polycythemia vera and essential thrombocythemia and to improve chemosensitivity in chronic myeloid leukemia patients. However, the mechanisms by which IFNα affects disease-initiating hematopoietic stem and progenitor cells (HSPCs) remain poorly understood. Although IFNα has been found to transiently impair quiescence of murine hematopoietic stem cells, its effects on human HSPCs have not been studied in vivo. Here, we compared bone marrow serially obtained from patients with myeloproliferative neoplasms before and during pegylated IFNα treatment against marrow serially obtained from patients on hydroxyurea. The percentage of HSPCs actively undergoing cell cycle was increased after pegylated IFNα treatment in a majority of patients compared with hydroxyurea-treated controls, suggesting that IFNα promotes cell division. Furthermore, transcriptional profiling revealed that cell cycle-associated genes were induced, whereas genes involved in HSPC quiescence were repressed during IFNα treatment. Compared with hydroxyurea-treated controls, pegylated IFNα-treated patients had similar numbers of HSPCs, but increased numbers of hematopoietic progenitors as determined by colony formation assay, indicating an increase in myeloid proliferation/differentiation. These effects occurred regardless of JAK2 mutational status. Together, these data provide the first in vivo evidence that pegylated IFNα promotes cell division and differentiation of human HSPCs.

journal_name

Exp Hematol

journal_title

Experimental hematology

authors

King KY,Matatall KA,Shen CC,Goodell MA,Swierczek SI,Prchal JT

doi

10.1016/j.exphem.2015.05.013

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

912-918.e2

issue

10

eissn

0301-472X

issn

1873-2399

pii

S0301-472X(15)00195-2

journal_volume

43

pub_type

杂志文章

相关文献

EXPERIMENTAL HEMATOLOGY文献大全
  • Differential effects of mixed lymphocyte reaction supernatant on human mesenchymal stromal cells.

    abstract：:The concept that mesenchymal stromal cells (MSCs), a component of the hematopoietic microenvironment, can be a target for alloreactive effector cells in the context of graft-vs-host disease has not been investigated in detail. Mixed lymphocyte reaction (MLR) supernatant was used to mimic the inflammatory milieu induce...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2012.07.011

    authors： Fasslrinner F,Wobus M,Duryagina R,Müller K,Stopp S,Wehner R,Rauner M,Hofbauer LC,Schmitz M,Bornhäuser M

    更新日期：2012-11-01 00:00:00

  • Differential engraftment of genetically modified CD34(+) and CD34(-) hematopoietic cell subsets in lethally irradiated baboons.

    abstract：OBJECTIVE:To test gibbon ape leukemia virus (GALV) pseudotype vector transduction of marrow subpopulations that contribute to hematopoietic reconstitution in vivo. MATERIALS AND METHODS:Autologous CD34(+) Lin(-), CD34(+) Lin(+), and CD34(-) Lin(-) marrow cells, transduced by coculture with PG13/LN, PG13/LNX, and PG13/...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(00)00137-5

    authors： Andrews RG,Peterson LJ,Morris J,Potter J,Heyward S,Gough M,Bryant E,Kiem HP

    更新日期：2000-05-01 00:00:00

  • Differential effect of adenosine on DNA synthesis in lymphoid and myeloid cell lines.

    abstract：:In this report, the effects of adenosine on the promyelocytic cell line HL-60 and on T-lymphocytic clones are compared. According to previous reports, adenosine induces a dose-dependent inhibition of DNA synthesis in T-lymphocytes. Conversely, adenosine dose-dependently enhances DNA synthesis in HL-60 cells, as docume...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Orrico A,Capecchi PL,De Magistris T,Nuti S,Pasini FL

    更新日期：1991-11-01 00:00:00

  • Constitutive and virus-induced interferon production by peripheral blood leukocytes.

    abstract：:The production of interferon-alpha (IFN-alpha) by normal human peripheral blood mononuclear cells (PBMNC) was studied using polyclonal antipeptide antibodies designed to react either with all IFN-alpha subtypes or with individual subtypes IFN-alpha 2 or IFN-alpha 4. In this study, we demonstrate the detection of intra...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Greenway AL,Hertzog PJ,Devenish RJ,Linnane AW

    更新日期：1995-03-01 00:00:00

  • Blast colony-forming cells and precursors of colony-forming cells detectable in long-term marrow culture express the same phenotype (CD33- CD34+).

    abstract：:We have previously shown that CD33- CD34+ human marrow cells are capable of giving rise to colony-forming cells (CFC) in long-term marrow culture (LTMC) but are mainly depleted of progenitors that directly form colonies in semi-solid media. In contrast, the CD33+ CD34+ population contains most of the CFC but not the p...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Bernstein ID,Leary AG,Andrews RG,Ogawa M

    更新日期：1991-08-01 00:00:00

  • Differences in irradiation susceptibility and turnover between mucosal and connective tissue-type mast cells of mice.

    abstract：:Although precursors of mast cells are derived from the bone marrow, phenotypes of mast cells are influenced by the tissues in which final differentiation occurs. Connective tissue-type mast cells (CTMC) and mucosal mast cells (MMC) are different in morphological, biochemical, immunological, and functional criteria. Th...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Fukuzumi T,Waki N,Kanakura Y,Nagoshi J,Hirota S,Yoshikawa K,Kitamura Y

    更新日期：1990-08-01 00:00:00

  • Induced pluripotent stem cell modeling of malignant hematopoiesis.

    abstract：:The ability to epigenetically reprogram differentiated somatic cells to pluripotency resulting in the discovery of induced pluripotent stem cells (iPSCs), has unlocked fundamental biologic insights into numerous genetic diseases. These insights have resulted from the key property of iPSCs to differentiate into all cel...

    journal_title：Experimental hematology

    pub_type： 杂志文章,评审

    doi：10.1016/j.exphem.2019.01.002

    authors： Chao MP,Majeti R

    更新日期：2019-03-01 00:00:00

  • MicroRNA expression dynamics during murine and human erythroid differentiation.

    abstract：OBJECTIVE:MicroRNAs (miRNAs) are an abundant class of small noncoding RNAs that regulate diverse cellular functions by sequence-specific inhibition of gene expression. We determined miRNA expression profile during erythroid differentiation and putative roles in erythroid differentiation. METHODS:The expression profile...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2007.03.014

    authors： Zhan M,Miller CP,Papayannopoulou T,Stamatoyannopoulos G,Song CZ

    更新日期：2007-07-01 00:00:00

  • Mobilization by either cyclophosphamide or granulocyte colony-stimulating factor transforms the bone marrow into a highly proteolytic environment.

    abstract：OBJECTIVE:Hematopoietic stem and progenitor cells normally reside in the bone marrow but can be mobilized into the peripheral blood following treatment with granulocyte colony-stimulating factor (G-CSF) or myelosuppressive chemotherapy. Although the number of transplants performed with mobilized blood currently exceeds...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(02)00788-9

    authors： Lévesque JP,Hendy J,Takamatsu Y,Williams B,Winkler IG,Simmons PJ

    更新日期：2002-05-01 00:00:00

  • Acute myeloid leukemia cells in G0 phase of the cell cycle that are unresponsive to conventional chemotherapy are sensitive to treatment with granulocyte-macrophage colony-stimulating factor/diphtheria toxin fusion proteins.

    abstract：OBJECTIVE:Unresponsiveness to chemotherapy is a major problem in the treatment of leukemia, which can be caused by unresponsiveness of noncycling cells to cell cycle-dependent cytotoxic agents. Targeted toxins consisting of a targeting and activating cytokine (granulocyte-macrophage colony-stimulating factor [GM-CSF]) ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2003.11.005

    authors： Jedema I,Barge RM,Frankel AE,Willemze R,Falkenburg JH

    更新日期：2004-02-01 00:00:00

  • The anti-apoptotic gene Anamorsin is essential for both autonomous and extrinsic regulation of murine fetal liver hematopoiesis.

    abstract：:Anamorsin (AM) is an antiapoptotic molecule that confers factor-independent survival on hematopoietic cells. AM-deficient (AM(-/-)) mice are embryonic lethal because of a defect in definitive hematopoiesis; however, the significance of AM in embryonic hematopoiesis remains unknown. This study characterized the hematop...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2014.01.002

    authors： Tanimura A,Shibayama H,Hamanaka Y,Fujita N,Ishibashi T,Sudo T,Yokota T,Ezoe S,Tanaka H,Matsumura I,Oritani K,Kanakura Y

    更新日期：2014-05-01 00:00:00

  • Agonists to the A3 adenosine receptor induce G-CSF production via NF-kappaB activation: a new class of myeloprotective agents.

    abstract：OBJECTIVE:The aim of this study was to evaluate the effect of CF101, a synthetic agonist to the A3 adenosine receptor (A3AR), on the production of granulocyte colony-stimulating factor (G-CSF). The ability of CF101 to act as a myeloprotective agent in chemotherapy-treated mice was tested. METHODS:CF101 was administere...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(02)00962-1

    authors： Bar-Yehuda S,Madi L,Barak D,Mittelman M,Ardon E,Ochaion A,Cohn S,Fishman P

    更新日期：2002-12-01 00:00:00

  • Cytotoxic and biochemical effects of 3,3',4,4',5,5'-hexahydroxystilbene, a novel resveratrol analog in HL-60 human promyelocytic leukemia cells.

    abstract：OBJECTIVE:Resveratrol (3,4',5,-trihydroxystilbene, RV), an ingredient of wine, is an inhibitor of the proliferation-linked enzyme ribonucleotide reductase (RR) and shows a broad spectrum of cytotoxic effects against human cancer cells. In order to enhance these effects, we introduced additional hydroxyl moieties into t...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2006.05.018

    authors： Horvath Z,Murias M,Saiko P,Erker T,Handler N,Madlener S,Jaeger W,Grusch M,Fritzer-Szekeres M,Krupitza G,Szekeres T

    更新日期：2006-10-01 00:00:00

  • Mutagenicity of merocyanine 540-mediated photosensitization.

    abstract：:Merocyanine 540 (MC 540) is a photosensitizing dye with antineoplastic and antiviral properties. Because photoexcited MC 540 generates singlet molecular oxygen and possibly other reactive oxygen species there is concern that MC 540-mediated photosensitization may be mutagenic. In this paper, we report on the induction...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： O'Brien JM,Sieber F

    更新日期：1989-02-01 00:00:00

  • Polycythemia vera erythroid precursors exhibit increased proliferation and apoptosis resistance associated with abnormal RAS and PI3K pathway activation.

    abstract：OBJECTIVE:Polycythemia vera (PV) is characterized by erythrocytosis associated with the presence of the activating JAK2(V617F) mutation in a variable proportion of hematopoietic cells. JAK2(V617F) is detected in other myeloproliferative neoplasms, does not appear to be the PV-initiating event, and its specific role in ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2009.09.009

    authors： Laubach JP,Fu P,Jiang X,Salter KH,Potti A,Arcasoy MO

    更新日期：2009-12-01 00:00:00

  • Effect of androgens on maturation and metabolism of erythroid tissue.

    abstract：:Specific changes taking place in the erythroid tissue following depletion or replacement of androgens were studied in rats. The reduction of testosterone levels in blood of orchiectomized animals did occur in conjunction with a decline of erythrocyte glucose-6-phosphate (G6P) and lactate levels. No evidence of anemia ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Molinari PF,Esber HJ,Snyder LM

    更新日期：1976-09-01 00:00:00

  • Long-term repopulating abilities of enriched fetal liver stem cells measured by competitive repopulation.

    abstract：:To characterize hematopoietic cell biology, many investigators have used protocols that enrich for primitive hematopoietic stem cells (PHSC). In this study, we quantified the long-term repopulating ability (LTRA) of enriched and discarded fractions of PHSC from day-14 murine fetal liver using the competitive repopulat...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Jordan CT,Astle CM,Zawadzki J,Mackarehtschian K,Lemischka IR,Harrison DE

    更新日期：1995-08-01 00:00:00

  • Identification of Ponatinib as a potent inhibitor of growth, migration, and activation of neoplastic eosinophils carrying FIP1L1-PDGFRA.

    abstract：:In chronic eosinophilic leukemia, the transforming oncoprotein FIP1L1-PDGFRA is a major target of therapy. In most patients, the tyrosine kinase inhibitor (TKI) imatinib induces complete remission. For patients who are intolerant or resistant, novel TKIs have been proposed. We examined the in vitro effects of 14 kinas...

    journal_title：Experimental hematology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.exphem.2013.12.007

    authors： Sadovnik I,Lierman E,Peter B,Herrmann H,Suppan V,Stefanzl G,Haas O,Lion T,Pickl W,Cools J,Vandenberghe P,Valent P

    更新日期：2014-04-01 00:00:00

  • T-cell P/E-selectin ligand alpha(1,3)fucosylation is not required for graft-vs-host disease induction.

    abstract：OBJECTIVE:Recognition of E- and P-selectins on vascular endothelium by their leukocyte glycoprotein counterreceptor P-selectin glycoprotein ligand-1 (PSGL-1) initiates and sustains leukocyte rolling, culminating in extravasation of lymphocytes from blood into organs. PSGL-1 is rendered functional by terminal glycosylat...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2005.08.003

    authors： Eom HS,Rubio MT,Means TK,Luster AD,Sykes M

    更新日期：2005-12-01 00:00:00

  • The high affinity Fc gamma receptor (CD64) induces phagocytosis in the absence of its cytoplasmic domain: the gamma subunit of Fc gamma RIIIA imparts phagocytic function to Fc gamma RI.

    abstract：:The high affinity Fc gamma receptor, Fc gamma RI, is unique among the three classes of macrophage Fc gamma receptors not only in its affinity for IgG, but also in the structure of its cytoplasmic domain. Fc gamma RIIA and the gamma subunit of Fc gamma RIIIA have tyrosine-containing motifs within their cytoplasmic doma...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Indik ZK,Hunter S,Huang MM,Pan XQ,Chien P,Kelly C,Levinson AI,Kimberly RP,Schreiber AD

    更新日期：1994-07-01 00:00:00

  • Absence of both subunits of cytochrome b558 in the UM384 cell line relative to the inability to generate superoxide anions.

    abstract：:Phagocytic cells are characterized by their ability to generate superoxide anions upon activation by appropriate stimuli. UM384, a myelomonocytic cell line, was shown to be defective in this oxidase activity as measured by nitroblue tetrazolium or cytochrome c reduction. Cytochrome b558, a unique pigment present in ph...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Champelovier P,Laporte F,Verhoeven AJ,Hilarius P,de Klein A,Revol C,Seigneurin D,Kolodie L

    更新日期：1993-07-01 00:00:00

  • Identification of hematopoietic-specific regulatory elements from the CD45 gene and use for lentiviral tracking of transplanted cells.

    abstract：:The development of a hematopoietic reporter is crucial for determining the fate of lineages derived from cell-based therapies. A marking system will enable safer embryonic stem and induced pluripotent stem cell-based derivation of blood lineages and facilitate the development of efficient cellular reprogramming strate...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2014.05.005

    authors： Duong KL,Das S,Yu S,Barr JY,Jena S,Kim E,Zavazava N,Colgan JD,Xue HH,Levasseur DN

    更新日期：2014-09-01 00:00:00

  • T-cell apoptosis induced by granulocyte colony-stimulating factor is associated with retinoblastoma protein phosphorylation and reduced expression of cyclin-dependent kinase inhibitors.

    abstract：:Peripheral blood progenitor cells (PBPC) mobilized by granulocyte colony-stimulating factor (G-CSF) promptly engraft allogeneic recipients after myeloablative chemotherapy for hematologic malignancies. Surprisingly, no exacerbation of acute graft-vs-host disease has been observed despite a 10-fold higher T-cell conten...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(01)00617-8

    authors： Rutella S,Pierelli L,Rumi C,Bonanno G,Marone M,Sica S,Capoluongo E,Ameglio F,Scambia G,Leone G

    更新日期：2001-04-01 00:00:00

  • Human osteosarcoma cell lines MG-63 and SaOS-2 produce G-CSF and GM-CSF: identification and partial characterization of cell-associated isoforms.

    abstract：:Bone marrow stem cells reside in close proximity to endosteal osteoblasts. To explore the potential role of osteoblasts in hematopoietic differentiation, we measured the mRNA accumulation, protein production, and secretion of hematopoietic growth factors by the nonmineralizing MG-63 and the mineralizing SaOS-2 human o...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Taichman RS,Emerson SG

    更新日期：1996-03-01 00:00:00

  • Evaluation of Fc-dependent monocyte-macrophage function in bone marrow transplant recipients.

    abstract：:Allogeneic bone marrow recipients exhibit a complex and multifactorial immunodeficiency affecting numerous effector functions for various periods of time. In order to evaluate their Fc-mediated monocyte-macrophage (MM) function we studied the clearance of IgG-coated Rh-positive erythrocytes (EA) in healthy control sub...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Brochu S,Perreault C,Bélanger R

    更新日期：1989-10-01 00:00:00

  • Soluble c-kit receptor mobilizes hematopoietic stem cells to peripheral blood in mice.

    abstract：OBJECTIVE:The mechanisms of mobilization of hematopoietic stem cells (HSC) from bone marrow to peripheral blood (PB) by cytokines are poorly understood. One hypothesis is that cytokines disrupt cytoadhesive interactions of stem cells with bone marrow stroma. The soluble portion of c-kit (s-kit) binds stem cell factor (...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2004.01.004

    authors： Nakamura Y,Tajima F,Ishiga K,Yamazaki H,Oshimura M,Shiota G,Murawaki Y

    更新日期：2004-04-01 00:00:00

  • Bcl-x is a regulatory factor of apoptosis and differentiation in megakaryocytic lineage cells.

    abstract：:Differentiation- and lineage-related differences in the expression of two anti-apoptotic molecules, bcl-x and bcl-2, were examined using various human hematopoietic cell lines. Bcl-x was strongly expressed in cell lines with erythroid and megakaryocytic properties (K562, HEL, CMK, and Mo7E), and was moderately express...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Terui Y,Furukawa Y,Kikuchi J,Iwase S,Hatake K,Miura Y

    更新日期：1998-03-01 00:00:00

  • Effects of Cis-diamminedichloroplatinum (II) on hematopoietic stem cells in mice.

    abstract：:Bone marrow cell responses to therapeutic doses of cis-diamminedichloroplatinum (II) (Cis-Pt) were evaluated in female C57B1/6 mice by determining bone marrow cellularity and content of transplantable colony forming units (CFUS) after treatment. Although limitation on the dosage of Cis-Pt is generally considered to be...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Jenkins VK,Perry RR,Goodrich WE

    更新日期：1981-03-01 00:00:00

  • Hypoxia-inducible factor-1 drives the motility of the erythroid progenitor cell line, UT-7/Epo, via autocrine motility factor.

    abstract：OBJECTIVE:It is well known that hypoxic stress strongly enhances erythropoiesis, but the effect of hypoxia on erythroid progenitors has not been examined precisely. In the present study, using the erythropoietin-dependent cell line UT-7/Epo, which has characteristics of erythroid progenitors, we investigated a novel ro...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2005.01.013

    authors： Mikami M,Sadahira Y,Haga A,Otsuki T,Wada H,Sugihara T

    更新日期：2005-05-01 00:00:00

  • Single-Cell Transcriptome in Chronic Myeloid Leukemia: Pseudotime Analysis Reveals Evidence of Embryonic and Transitional Stem Cell States.

    abstract：:Recent experimental data suggest that the heterogeneity of chronic myeloid leukemia (CML) stem cells may be the result of the development of unique molecular events generating functional consequences in terms of the resistance and persistence of leukemic stem cells. To explore this phenomenon, we designed a single-cel...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2020.04.005

    authors： Pagliaro S,Desterke C,Acloque H,Chomel JC,de Souza L,Hugues P,Griscelli F,Foudi A,Bennaceur-Griscelli A,Turhan AG

    更新日期：2020-05-01 00:00:00