Abstract：
:Bone marrow cell responses to therapeutic doses of cis-diamminedichloroplatinum (II) (Cis-Pt) were evaluated in female C57B1/6 mice by determining bone marrow cellularity and content of transplantable colony forming units (CFUS) after treatment. Although limitation on the dosage of Cis-Pt is generally considered to be determined by renal toxicity, methods to increase the effective use of the drug such as hydration and diuresis, alteration of time-dose schedules and combination of Cis-Pt with other drugs and radiation, warrant studies on effects of the drug on other normal tissues such as bone marrow. Cis-Pt given on a time-dose schedule that is therapeutic for mice and, in general, is equivalent to regimens recommended for clinical use, was found to be myelosuppressive. The myelosuppression was dose related in that a total dose of 16 mg/kg (2 doses at weekly interval) reduced the number of CFUS/femur to 16% of the control value, 24 mg/kg (4 doses at weekly intervals) reduced the CFUS fraction to 10-14% of the value for controls and 32 mg/kg (4 doses at weekly intervals) reduced the fraction of CFUs to 6-7% of the control value. A quantitative extrapolation of these effects to man cannot be made, however, the data indicate that myelosuppression could become a significant factor as increased doses of Cis-Pt are used clinically.
journal_name
Exp Hematoljournal_title
Experimental hematologyauthors
Jenkins VK,Perry RR,Goodrich WEsubject
Has Abstractpub_date
1981-03-01 00:00:00pages
281-7issue
3eissn
0301-472Xissn
1873-2399journal_volume
9pub_type
杂志文章abstract：:We previously demonstrated that lysis of tumor cells that express Hsp70, the highly stress-inducible member of the HSP70 family, on their plasma membrane is mediated by natural killer (NK) cells. Here, we studied the effects of different proteins of the HSP70 family in combination with interleukin 2 (IL-2) on the prol...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(99)00104-6
更新日期：1999-11-01 00:00:00
abstract：:The history of clonal hematopoiesis (CH) research is punctuated by several seminal discoveries that have forged our understanding of cancer development. The clever application of the principle of random X-chromosome inactivation (XCI) in females led to the development of the first test to identify clonal derivation of...
journal_title：Experimental hematology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.exphem.2020.01.008
更新日期：2020-03-01 00:00:00
abstract：:Certain biological effects exerted by 4-hydroperoxycyclophosphamide and by 2,4-tetrahydrocyclohexylamine (ASTA-Z-7557), utilized in vitro in the therapy of leukemias and lymphomas to eliminate the occult tumor cells in autologous marrow transplantations, were studied in human lymphocytes cultured in vitro. The data sh...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
abstract：OBJECTIVE:Several compounds, including butyrate and trichostatin A, have been shown to activate gamma-gene expression via p38 mitogen-activated protein kinase (MAPK) signaling. In eukaryotic cells, reactive oxygen species (ROS) act as signaling molecules to mediate phosphorylation of tyrosine kinases such as p38 MAPK t...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2005.12.009
更新日期：2006-03-01 00:00:00
abstract：:Aplastic anemia serum (AAS) contains humoral factors that alter both proliferation and maturation of human megakaryocytes (MK). The ability of AAS to augment MK colony formation (colony-forming unit, CFU-MK) was neutralized by an antiserum against MK colony-stimulating factor (MK-CSF), a glycoprotein isolated from AAS...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:Bone marrow and/or peripheral blood of patients with chronic myeloid leukemia (CML) was investigated by the following three parameters: Ph' chromosome, bcr-abl expression in fresh blood and/or bone marrow, and bcr-abl expression in single hematopoietic progenitor colonies generated from blood and/or bone marrow. Expre...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:More than 2million human erythroblasts extrude their nuclei every second in bone marrow under hypoxic conditions (<7% O2). Enucleation requires specific signal transduction pathways and the local assembly of contractile actomyosin rings. However, the energy source driving these events has not yet been identified. We e...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2019.02.004
更新日期：2019-04-01 00:00:00
abstract：:Electroporation has recently been shown to have advantages over the commonly used method of calcium phosphate precipitation for obtaining DNA-mediated transformation of certain types of cells. Although mouse erythroleukemia cells and other cells of hematopoietic origin are not transformed at useful frequencies by calc...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
abstract：:The cycle specific drug, hydroxyurea (HU), has previously been used to study the proliferative kinetics of various hemopoietic precursor cells such as granulocyte-macrophage precursors (CFUc) and multipotential stem cells (CFUs). We have investigated the kinetics of another hemopoietic precursor, a T-cell colony (CFUT...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1980-05-01 00:00:00
abstract：OBJECTIVE:Differentiation-inducing therapy by agents such as 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) represents a useful approach for the treatment of acute myelogenous leukemia (AML). We previously showed that Gemini-23-yne-26,27-hexafluoro-D(3) inhibited the proliferation of MCF-7 breast cancer cells in associat...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2010.03.020
更新日期：2010-08-01 00:00:00
abstract：:Patients who undergo autologous bone marrow transplantation for acute leukemia are at high risk for relapse. We have evaluated the feasibility of administering cell-mediated immunotherapy with family-related haploidentical lymphocytes following autologous bone marrow transplantation in order to evoke a graft-vs-leukem...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(00)00533-6
更新日期：2000-11-01 00:00:00
abstract：:We investigated the interactions between the bone marrow microenvironment and T cell populations at different stages of maturation. Thymocytes were seeded onto confluent layers of bone marrow stromal cell lines (MBA-13 or 14F1.1). Within a few hours two main thymocyte populations were observed; one remained in the liq...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:Mantle cell lymphoma (MCL) affects approximately 4500 patients/year in the US and demonstrates a male to female ratio of approximately 4:1. While the pathobiology underlying this ratio is unknown, the hematopoietic system is characterized by sex-related differences in androgen receptor (AR) expression, leading us to h...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2017.01.001
更新日期：2017-05-01 00:00:00
abstract：OBJECTIVES:To analyze the consequences of lymphocyte donations on lymphopoiesis in donors having previously undergone hematopoietic stem cell collection for allogeneic stem cell transplantation. METHODS:Repeated analysis of leukocyte subsets in the peripheral blood of 76 hematopoietic stem cell donors undergoing lymph...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2004.07.021
更新日期：2004-11-01 00:00:00
abstract：:Thrombocytopenia is an important clinical problem for many acquired immunodeficiency syndrome (AIDS) patients. Recently, the utility of recombinant cytokines in alleviating the hematopoietic complications of AIDS and AIDS therapy has been evaluated. The newly cloned cytokine stem cell factor (SCF) has been demonstrate...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：OBJECTIVE:To assess the contribution of bone marrow (BM)-derived endothelial progenitor cells (EPCs) to the neovascularisation of cutaneous incisional wounds. METHODS:Lethally irradiated C57Bl/6 mice were transplanted with BM mononuclear cells from Tie2/lacZ mice, which constitutively overexpressed beta-galactosidase ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2006.10.016
更新日期：2007-03-01 00:00:00
abstract：:Germinal center-associated nuclear protein (GANP) is a unique and multifunctional protein that plays a critical role in cell biology, neurodegenerative disorders, immunohematology, and oncogenesis. GANP is an orthologue of Saccharomyces Sac3, one of the components of the transcription export 2 (TREX-2) complex and a m...
journal_title：Experimental hematology
pub_type： 杂志文章,评审
doi：10.1016/j.exphem.2020.08.007
更新日期：2020-10-01 00:00:00
abstract：:The bcr/abl transcript is specifically expressed in the hematopoietic cells of patients with chronic myeloid leukemia (CML). The Haseloff and Gerlach model was used to design a ribozyme targeted to this transcript. When tested against a synthetic substrate covering the translocation sequence, the ribozyme exhibited si...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:Chronic lymphocytic leukemia (CLL) is a heterogeneous disease characterized by defects in the DNA damage response and apoptosis. Among the factors involved in these pathways, we focused on the enzyme poly(ADP-ribose) polymerase 1 (PARP1) and on its substrate Che-1 by evaluating their basal expression and functional ch...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2011.11.005
更新日期：2012-03-01 00:00:00
abstract：:We present the first report of a T-helper cell line, HUT 102, constitutively producing both granulocyte-macrophage colony-stimulating factor (GM-CSF) and monocytic colony-stimulating factor (M-CSF), as detected by sensitive enzyme-linked immunosorbent assays and Northern blot analysis. However, neither amplification n...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:Allogeneic bone marrow recipients exhibit a complex and multifactorial immunodeficiency affecting numerous effector functions for various periods of time. In order to evaluate their Fc-mediated monocyte-macrophage (MM) function we studied the clearance of IgG-coated Rh-positive erythrocytes (EA) in healthy control sub...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Our previous studies have shown that a unique glycoprotease from Pasteurella haemolytica specifically cleaves only proteins containing sialylated O-linked glycans. The hematopoietic progenitor cell antigen, CD34, which is heavily glycosylated with both N- and O-linked glycans, is readily cleaved by this protease. In t...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:The granulocyte defects of Chediak-Higashi syndrome (CHS) include neutropenia, characteristic giant lysosomal granule morphology, and functionally abnormal cell motility, degranulation, and bacterial killing. Findings of elevated levels of adenosine 3', 5' cyclic monophosphate nucleotide (cAMP) and of concanavalin A (...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1985-02-01 00:00:00
abstract：OBJECTIVES:GPIIb integrin expression has been found on platelets and megakaryocytes, and more recently on immature hematopoietic progenitors. We set out to investigate expression of GPIIb in other hematopoietic cell lineages and, having detected it on mast cells, aimed to determine what possible role it might perform. ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2005.01.011
更新日期：2005-04-01 00:00:00
abstract：:The kinetics of cell turnover for myeloid/monocyte cells that form colonies in agar (GM-CFC) were measured through the progressive increase in their sensitivity to 313-nm light during a period of cell labeling with BrdCyd. Two components of cell killing with distinctly separate labeling kinetics revealed both the pres...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:Mast cell (MC)-fibroblast-immunocompetent cell interactions may play a role in the inflammatory and fibrotic processes present in chronic graft-vs.-host disease (cGVHD). Interactions between these cell types were examined in both murine and human cGVHD models. To this purpose, cell supernatants from mice or humans wit...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：OBJECTIVES:To characterize and evaluate the validity of a novel coculture system for studying human B-lymphocyte developmental biology. MATERIALS AND METHODS:We developed a long-term culture system to produce B lymphocytes from human CD34(+) cells purified from umbilical cord blood using human mesenchymal stem cells (...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2007.12.013
更新日期：2008-05-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to evaluate the repopulating properties of bone marrow (BM) from mice irradiated during embryonic and adult stages of development. MATERIALS AND METHODS:Four-day-old embryos, 17-day-old fetuses, and 12-week-old mice were irradiated with of 1 or 3 Gy of x-rays. At 3 and 9 months ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(99)00128-9
更新日期：2000-01-01 00:00:00
abstract：OBJECTIVE:The hematopoietic system is nurtured by a supportive stroma environment allowing maintenance and differentiation of hematopoietic stem cells (HSC). However, only a limited number of these stromal cell clones support hematopoiesis in the absence of cytokine supplementation. So far, only two bone marrow-derived...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2006.06.013
更新日期：2006-11-01 00:00:00
abstract：:Reports that cells from neonatal animals produce CFU-E in response to erythropoietin (Ep) at doses lower than are required by adults indicated that young mice would provide a good system to study the hormone response of erythropoietic progenitors. Studies presented here confirm the increased Ep responsiveness until 3-...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
