当前位置： SCI文献检索 > EXPERIMENTAL HEMATOLOGY期刊下所有文献 > Adoptive immunotherapy with haploidentical allogeneic peripheral blood lymphocytes following autologous bone marrow transplantation.

Adoptive immunotherapy with haploidentical allogeneic peripheral blood lymphocytes following autologous bone marrow transplantation.

Abstract：

:Patients who undergo autologous bone marrow transplantation for acute leukemia are at high risk for relapse. We have evaluated the feasibility of administering cell-mediated immunotherapy with family-related haploidentical lymphocytes following autologous bone marrow transplantation in order to evoke a graft-vs-leukemia effect in the autologous setting.Twenty-six patients aged 1.5-48 years were enrolled in this study. Eighteen suffered from acute myeloid leukemia, seven from acute lymphoblastic leukemia, and one from myelodysplastic syndrome. Eleven patients were transplanted in first remission, six in second remission, one in fourth remission, and eight in relapse. Conditioning consisted of Busulfan/Cyclophosphamide or Busulfan/Thiotepa/Cyclophosphamide. Nineteen patients (Group A) were treated with gradual increments of haploidentical donor T cells, starting on day +1, with an additional course of T cells plus intravenous recombinant human interleukin-2 one month later if no signs of graft-vs-host disease developed in the interim. Seven patients (Group B) were treated with high-dose haploidentical T cells on day +1 in conjunction with intravenous recombinant human interleukin-2. Donor cells were detected in the peripheral blood of both groups 12-48 hours post-cell-mediated immunotherapy, peaking at 48 hours. Three patients in Group A developed transient Grade I graft-vs-host disease. One patient in Group B developed Grade I, and three Grade IV, graft-vs-host disease. Group A patients engrafted normally, but the Group B patients with Grade IV graft-vs-host disease showed no signs of engraftment. Our results show that it is feasible to induce graft-vs-host disease in the autologous stem cell transplantation setting. However, the high-dose regimen of haploidentical T cells in conjunction with interleukin-2 results in severe toxicity and nonengraftment.

journal_name

Exp Hematol

journal_title

Experimental hematology

authors

Nagler A,Ackerstein A,Or R,Naparstek E,Slavin S

doi

10.1016/s0301-472x(00)00533-6

keywords:

subject

Has Abstract

pub_date

2000-11-01 00:00:00

pages

1225-31

issue

11

eissn

0301-472X

issn

1873-2399

pii

S0301-472X(00)00533-6

journal_volume

28

pub_type

杂志文章

相关文献

EXPERIMENTAL HEMATOLOGY文献大全
  • Physical interaction between BAALC and DBN1 induces chemoresistance in leukemia.

    abstract：:BAALC is identified as a leukemia-associated gene and is highly expressed in CD34-positive hematopoietic stem cells. High BAALC expression is associated with poor prognosis in several types of acute myeloid leukemia. We explored binding partner proteins of BAALC by means of mass spectrometry and analyzed biological pr...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2020.12.003

    authors： Maki H,Yoshimi A,Shimada T,Arai S,Morita K,Kamikubo Y,Ikegawa M,Kurokawa M

    更新日期：2021-01-14 00:00:00

  • Stem cell plasticity: recapping the decade, mapping the future.

    abstract：:In slightly more than a decade of stem cell plasticity research, 24 peer-reviewed articles have demonstrated plasticity across organ and/or embryonic lineage boundaries at the single-cell level, with only 1 article showing negative results. These data, taken together with data about reversibility of gene restrictions ...

    journal_title：Experimental hematology

    pub_type： 杂志文章,评审

    doi：10.1016/j.exphem.2010.04.013

    authors： Theise ND

    更新日期：2010-07-01 00:00:00

  • Vascular cell adhesion molecule-1 and VLA-4 are obligatory adhesion proteins in the heterotypic adherence between human leukemia/lymphoma cells and marrow stromal cells.

    abstract：:We have utilized two well-characterized human leukemia/lymphoma (LL) cell lines, UTMB-460 and CEM, to determine the role of integrin very late antigen-alpha 4 beta 1 (VLA-4) and its ligand vascular cell adhesion molecule-1 (VCAM-1) in the adherence of the LL cells to marrow stromal cells (MSC). Both these LL cell line...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Juneja HS,Schmalsteig FC,Lee S,Chen J

    更新日期：1993-03-01 00:00:00

  • Preparation of human erythropoietin for tissue culture.

    abstract：:A simple two-step procedure has been developed for the production of human urinary erythropoietin suitable for tissue culture use. Lyophilized human urinary protein was fractionated by phenyl-Sepharose chromatography, the erythropoietin binding in 2 M lithium chloride and being eluted by an ascending linear gradient t...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Cutler RL,Johnson GR,Nicola NA

    更新日期：1985-09-01 00:00:00

  • Marrow repopulating cells, but not CFU-S, establish long-term in vitro hemopoiesis on a marrow-derived stromal layer.

    abstract：:We have studied the ability of subpopulations of hemopoietic stem cells, obtained from murine bone marrow using elutriation and multiparameter sorting, to establish and maintain hemopoiesis following their deposition on irradiated stromal layers of long-term bone marrow cultures. Two fractions were obtained that diffe...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： van der Sluijs JP,de Jong JP,Brons NH,Ploemacher RE

    更新日期：1990-09-01 00:00:00

  • A randomized multicenter comparison of CD34(+)-selected progenitor cells from blood vs from bone marrow in recipients of HLA-identical allogeneic transplants for hematological malignancies.

    abstract：OBJECTIVE:Peripheral blood progenitor cells (PBPC) have been established as an alternative source of hematopoietic stem cells for allogeneic transplantation, but an increased incidence of both acute and chronic graft-vs-host disease (GVHD) has become apparent. We performed a prospective randomized trial comparing bone ...

    journal_title：Experimental hematology

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1016/s0301-472x(03)00195-4

    authors： Cornelissen JJ,van der Holt B,Petersen EJ,Vindelov L,Russel CA,Höglund M,Maertens J,Schouten HC,Braakman E,Steijaert MM,Zijlmans MJ,Slaper-Cortenbach I,Boogaerts MA,Löwenberg B,Verdonck LF

    更新日期：2003-10-01 00:00:00

  • The polo-like kinase inhibitor BI 2536 exhibits potent activity against malignant plasma cells and represents a novel therapy in multiple myeloma.

    abstract：OBJECTIVE:Polo-like kinase 1 (Plk1) is a regulator of the cell cycle that has been implicated in the pathology of many cancers. We have investigated whether this kinase plays a role in multiple myeloma (MM) using the Plk1 inhibitor BI 2536. MATERIALS AND METHODS:We have used six MM cell lines and six patient-derived s...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2010.12.006

    authors： Stewart HJ,Kishikova L,Powell FL,Wheatley SP,Chevassut TJ

    更新日期：2011-03-01 00:00:00

  • Antibodies to the beta 1-integrin chain, CD44, or ICAM-3 stimulate adhesion of blast colony-forming cells and may inhibit their growth.

    abstract：:Early hematopoietic progenitor cells adhere to bone marrow stromal cells (BMSCs) mainly through VLA-4/VCAM-1 interactions. However, many adhesion molecules are expressed by these cell types. Cell adhesion molecules not only mediate adhesion; some are also capable of triggering cellular signaling events. These signals ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Oostendorp RA,Spitzer E,Reisbach G,Dörmer P

    更新日期：1997-04-01 00:00:00

  • Erythropoiesis in the X-irradiated, bled rat.

    abstract：:Exposure to low levels of x-irradiation (50R) followed by phlebotomy (50% blood volume) one month post-irradiation, resulted in identifiable alterations of the erythropoietic status of the rat. The red cell indices revealed a decrease in mean corpuscular volume and an increase in mean corpuscular hemoglobin concentrat...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Marchetti DL,Glomski CA

    更新日期：1975-11-01 00:00:00

  • Temporal thrombocytopenia after engraftment with defined stem cells with long-term marrow reconstituting activity.

    abstract：:Delayed platelet recovery and altered marrow megakaryocytopoiesis was observed in highly irradiated mice following transplantation with 1000 lineage-negative, stem cell antigen-positive (Lin-Sca-1+) cells. Thirty days after transplantation, the mice were thrombocytopenic. Normal platelet levels were reestablished by 9...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Bradford G,Williams N,Barber L,Bertoncello I

    更新日期：1993-12-01 00:00:00

  • T-cell apoptosis induced by granulocyte colony-stimulating factor is associated with retinoblastoma protein phosphorylation and reduced expression of cyclin-dependent kinase inhibitors.

    abstract：:Peripheral blood progenitor cells (PBPC) mobilized by granulocyte colony-stimulating factor (G-CSF) promptly engraft allogeneic recipients after myeloablative chemotherapy for hematologic malignancies. Surprisingly, no exacerbation of acute graft-vs-host disease has been observed despite a 10-fold higher T-cell conten...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(01)00617-8

    authors： Rutella S,Pierelli L,Rumi C,Bonanno G,Marone M,Sica S,Capoluongo E,Ameglio F,Scambia G,Leone G

    更新日期：2001-04-01 00:00:00

  • Stages in the development of radiation-induced thymic lymphomas in C57 BL/Ka mice: preleukemic cells become progressively resistant to the tumor preventing effects of a bone marrow graft.

    abstract：:Fractionated whole body irradiation induces thymic lymphomas in C57 BL/Ka mice after 6-12 months. A graft of normal congenic bone marrow cells immediately after the last irradiation prevents the development of lymphomas by inducing the disappearance of preleukemic cells. When such a graft is performed one month later,...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Humblet C,Greimers R,Boniver J,Defrense MP

    更新日期：1997-02-01 00:00:00

  • Quantitative assessment of human T lymphocytes in RAG2(-/-)gammac(-/-) mice: the impact of ex vivo manipulation on in vivo functionality.

    abstract：OBJECTIVE:Recent clinical trials of adoptive immunotherapy showed diminished reactivity of human T cells upon ex vivo manipulation. For a safe and effective clinical application of human T cells, it is necessary to improve ex vivo manipulation procedures and evaluate their impact on in vivo functionality. However, ther...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2006.09.018

    authors： van Rijn RS,Simonetti ER,Hagenbeek A,Bonyhadi M,Storm G,Martens AC,Ebeling SB

    更新日期：2007-01-01 00:00:00

  • HIV-1 suppresses erythropoietin production in vitro.

    abstract：:Serum erythropoietin (Epo) levels are depressed in anemic AIDS patients relative to controls. The basis for abnormal regulation of Epo has not been defined. The hepatoma cell line Hep3B produces Epo in response to hypoxia and serves as a model for the study of Epo regulation. Hep3B cells are infectible with human immu...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Wang Z,Goldberg MA,Scadden DT

    更新日期：1993-05-01 00:00:00

  • The statistical distribution of colony counts in the spleen colony assay.

    abstract：:The statistical distribution of colony counts in the spleen colony assay was determined in C57BL/6 and C3H mice under varying conditions of the assay procedure. Assay mice were given syngeneic bone marrow cells from normal or irradiated donors. The mice were injected intravenously with marrow cells either immediately ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Mossman KL

    更新日期：1977-11-01 00:00:00

  • Human CD34+AC133+VEGFR-2+ cells are not endothelial progenitor cells but distinct, primitive hematopoietic progenitors.

    abstract：OBJECTIVE:Endothelial progenitor cells (EPCs) are used for angiogenic therapies or as biomarkers to assess cardiovascular disease risk. However, there is no uniform definition of an EPC, which confounds EPC studies. EPCs are widely described as cells that coexpress the cell-surface antigens CD34, AC133, and vascular en...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2007.04.002

    authors： Case J,Mead LE,Bessler WK,Prater D,White HA,Saadatzadeh MR,Bhavsar JR,Yoder MC,Haneline LS,Ingram DA

    更新日期：2007-07-01 00:00:00

  • Hemopoiesis in demineralized bone allografts.

    abstract：:Demineralized allogeneic bone matrix (DBM), implanted in muscle, induces the formation of an ossicle within which histologically recognizable hemopoietic tissue develops. Analyses of rabbit ossicle marrow in a methylcellulose culture system demonstrated the presence of committed hemopoietic precursors; colony-forming ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Brockbank KG,Ogawa M,Spector M

    更新日期：1980-07-01 00:00:00

  • The Dtk receptor tyrosine kinase, which binds protein S, is expressed during hematopoiesis.

    abstract：:Dtk (Tyro 3/Sky/Rse/Brt/Tif) belongs to a recently recognized subfamily of receptor tyrosine kinases that also includes Ufo (Axl/Ark) and Mer (Eyk). Ligands for Dtk and Ufo have been identified as protein S and the related molecule Gas6, respectively. This study examined expression of Dtk during ontogeny of the hemato...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Crosier PS,Freeman SA,Orlic D,Bodine DM,Crosier KE

    更新日期：1996-02-01 00:00:00

  • HIF-1α-stabilizing agent FG-4497 rescues human CD34+ cell mobilization in response to G-CSF in immunodeficient mice.

    abstract：:Granulocyte colony-stimulating factor (G-CSF) is used routinely in the clinical setting to mobilize hematopoietic stem progenitor cells (HSPCs) into the patient's blood for collection and subsequent transplantation. However, a significant proportion of patients who have previously received chemotherapy or radiotherapy...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2017.05.004

    authors： Nowlan B,Futrega K,Brunck ME,Walkinshaw G,Flippin LE,Doran MR,Levesque JP

    更新日期：2017-08-01 00:00:00

  • Inhibition of in vitro hematopoiesis by hepatitis A virus.

    abstract：:Inoculation of human bone marrow with hepatitis A virus (HAV) resulted in a dose- and duration-of-incubation-dependent suppression of hematopoietic progenitor (CFU-GM, BFU-E, CFU-Mix) growth in vitro. Monocytic progenitors appeared to be least affected. While HAV inactivation by heat or beta-propiolactone and neutrali...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Busch FW,de Vos S,Flehmig B,Herrmann F,Sandler C,Vallbracht A

    更新日期：1987-10-01 00:00:00

  • Terminal cytoplasmic maturation of human megakaryocytes in vitro.

    abstract：:Several studies suggest that serum factors (thrombopoietins) regulate thrombopoiesis by altering the number, size, ploidy, and maturation rate of megakaryocytes (MK). Various in vivo systems have been used to quantitate these events. In this study, an in vitro system was developed to monitor terminal cytoplasmic matur...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Straneva JE,Goheen MP,Hui SL,Bruno E,Hoffman R

    更新日期：1986-11-01 00:00:00

  • Immunological properties of bone marrow microenvironment 1 year after allogeneic hematopoietic stem cell transplantation.

    abstract：:Regeneration of the bone marrow microenvironment after transplantation of allogeneic hematopoietic stem cells is poorly explored. The goal of our study was to investigate this process focusing on immunologic factors: concentrations of selected cytokines, expression of immunosuppressive proteins CD47 and CD274 on hemat...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2016.08.001

    authors： Ciomber A,Mitrus I,Fidyk W,Smagur A,Chwieduk A,Glowala-Kosinska M,Czerw T,Sobczyk-Kruszelnicka M,Mendrek W,Sadus-Wojciechowska M,Najda J,Holowiecki J,Giebel S

    更新日期：2016-12-01 00:00:00

  • A new form of Filgrastim with sustained duration in vivo and enhanced ability to mobilize PBPC in both mice and humans.

    abstract：:Granulocyte colony-stimulating factor (G-CSF) has proven effective in the prophylaxis of chemotherapy-induced neutropenia and as a mobilizer of peripheral blood progenitor cells. The longevity of G-CSF action is limited by its removal from the body by two mechanisms. The first is thought to be mediated via receptors (...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(99)00112-5

    authors： Molineux G,Kinstler O,Briddell B,Hartley C,McElroy P,Kerzic P,Sutherland W,Stoney G,Kern B,Fletcher FA,Cohen A,Korach E,Ulich T,McNiece I,Lockbaum P,Miller-Messana MA,Gardner S,Hunt T,Schwab G

    更新日期：1999-12-01 00:00:00

  • Mechanism of the therapeutic effect of anisodamine in disseminated intravascular coagulation: study of platelet adhesion and aggregation, malondialdehyde, thromboxane B2, 6-keto-prostaglandin F1 alpha, and microcirculation.

    abstract：:Acute disseminated intravascular coagulation (DIC) is a life-threatening condition that may be encountered in many situations, especially in cases of shock with uncontrollable hemorrhage. Anisodamine, an alkaloid extracted from a Chinese herb, is well known for its dramatic therapeutic effect on DIC. Sixty male rabbit...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Zhang S,Chang AM,Li CF,Li ZJ,Yin ZJ,Zhao X,Liang SL

    更新日期：1987-01-01 00:00:00

  • Hematopoietic stem cells in +/+, nu/+, and nu/nu C57Bl/10 mice.

    abstract：:Mice with the nu mutation have been shown to have a reduced incidence of spleen colony-forming units (CFU-S), with a higher variability between individual mice compared to +/+ mice. The presence of these defects also in nu/+ mice indicates that this mutation is a codominant trait and that the hematopoietic changes may...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Necas E,Znojil V,Holub M

    更新日期：1991-08-01 00:00:00

  • Functional evaluation of ex vivo expanded cord blood lymphocytes: possible use for adoptive cellular immunotherapy.

    abstract：OBJECTIVE:To examine the possibility of adoptive cellular immunotherapy such as donor lymphocyte infusion using ex vivo expanded cord blood (CBL) lymphocytes, the potential expansion ability of CBL lymphocytes and the function of expanded CBL lymphocytes were evaluated. MATERIALS AND METHODS:Mononuclear cell fractions...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(02)00776-2

    authors： Azuma H,Yamada Y,Shibuya-Fujiwara N,Yamaguchi M,Murahashi H,Fujihara M,Sato N,Fukazawa K,Ikebuchi K,Ikeda H

    更新日期：2002-04-01 00:00:00

  • Osteoblasts promote engraftment of allogeneic hematopoietic stem cells.

    abstract：:Hematopoietic progenitor cells (low density, lineage negative [Lin-], depleted of CD4+, CD8+, B220+, Gr-1+, Mac-1+, CD71+, and Thy1.2+ cells) can engraft lethally irradiated mice when transplanted within, but not across the major histocompatibility (MHC) antigen barrier. We hypothesized that our progenitor cell prepar...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： El-Badri NS,Wang BY,Cherry,Good RA

    更新日期：1998-02-01 00:00:00

  • Human bone marrow progenitor cells stimulated by cord blood.

    abstract：:Agar cultures of human bone marrow cells stimulated by cord plasma or irradiated cord blood feeder layers demonstrated the presence of a multilineage hemopoietic growth factor in cord blood. When bone marrow cultures stimulated with giant cell tumor-conditioned medium (GCT-CM) were supplemented with this cord blood-de...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Yuen E,Brown RD,Kronenberg H,Rickard KA

    更新日期：1986-09-01 00:00:00

  • The time course of engraftment of human mesenchymal stem cells in fetal heart demonstrates that Purkinje fiber aggregates derive from a single cell and not multi-cell homing.

    abstract：OBJECTIVE:To study the early time course of engraftment of human mesenchymal stem cells in fetal sheep heart and determine the relative roles of proliferation and homing in formation of aggregates of human Purkinje fiber cells. METHODS:The human sheep xenograft model was utilized for these studies. Prior to injection ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2006.04.019

    authors： Colletti EJ,Almeida-Porada G,Chamberlain J,Zanjani ED,Airey JA

    更新日期：2006-07-01 00:00:00

  • The tissue inhibitor of metalloproteinases 1 increases the clonogenic efficiency of human hematopoietic progenitor cells through CD63/PI3K/Akt signaling.

    abstract：:Initially described as an endogenous inhibitor of proteases, the tissue inhibitor of metalloproteinases 1 (TIMP-1) also displays cytokine-like functions. TIMP-1 is a soluble protein whose levels are increased under inflammatory conditions. We recently found that TIMP-1(-/-) mice have decreased bone marrow (BM) cellula...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2015.07.003

    authors： Rossi L,Forte D,Migliardi G,Salvestrini V,Buzzi M,Ricciardi MR,Licchetta R,Tafuri A,Bicciato S,Cavo M,Catani L,Lemoli RM,Curti A

    更新日期：2015-11-01 00:00:00