abstract：:Precise replacement of diseased or dysfunctional organs is the goal of regenerative medicine and has appeared to be a distant goal for a long time. In the field of hematopoietic stem cell transplantation, this goal is now becoming tangible as gene-editing technologies and novel conditioning agents are entering the cli...

journal_title：Experimental hematology

authors： Russkamp NF,Myburgh R,Kiefer JD,Neri D,Manz MG

更新日期：2021-01-20 00:00:00

abstract：:BAALC is identified as a leukemia-associated gene and is highly expressed in CD34-positive hematopoietic stem cells. High BAALC expression is associated with poor prognosis in several types of acute myeloid leukemia. We explored binding partner proteins of BAALC by means of mass spectrometry and analyzed biological pr...

journal_title：Experimental hematology

authors： Maki H,Yoshimi A,Shimada T,Arai S,Morita K,Kamikubo Y,Ikegawa M,Kurokawa M

更新日期：2021-01-14 00:00:00

abstract：:The switch/sugar nonfermenting (SWI/SNF) family of chromatin remodeling complexes have been implicated in normal hematopoiesis. The ARID2 protein is a component of the polybromo-associated BAF (PBAF), one of the two main SWI/SNF complexes. In the current study, we used a conditional Arid2 knockout mouse model to deter...

journal_title：Experimental hematology

authors： Bluemn T,Schmitz J,Chen Y,Zheng Y,Zhang Y,Zheng S,Burns R,DeJong J,Christiansen L,Izaguirre-Carbonell J,Wang D,Zhu N

更新日期：2020-12-17 00:00:00

abstract：:Lifelong blood production is ensured by a population of rare and largely quiescent, long-lived hematopoietic stem cells (HSCs). The advent of single-cell technologies has recently highlighted underlying molecular and functional heterogeneity within the HSC pool. Despite heterogenous HSC behaviors, quiescence remains a...

journal_title：Experimental hematology

authors： Johnson C,Belluschi S,Laurenti E

更新日期：2020-12-01 00:00:00

abstract：:Decades-old findings supporting origin of F cells in adult life from adult-type progenitors and the in vitro and in vivo enhancement of fetal globin under stress conditions have been juxtaposed against recent mechanistic underpinnings. An updated molecular interrogation did not debunk prior conclusions on the origin o...

journal_title：Experimental hematology

authors： Papayannopoulou T

更新日期：2020-12-01 00:00:00

abstract：:Germinal center-associated nuclear protein (GANP) is a unique and multifunctional protein that plays a critical role in cell biology, neurodegenerative disorders, immunohematology, and oncogenesis. GANP is an orthologue of Saccharomyces Sac3, one of the components of the transcription export 2 (TREX-2) complex and a m...

journal_title：Experimental hematology

authors： Sakai Y,Phimsen S,Okada S,Kuwahara K

更新日期：2020-10-01 00:00:00

abstract：:Recent experimental data suggest that the heterogeneity of chronic myeloid leukemia (CML) stem cells may be the result of the development of unique molecular events generating functional consequences in terms of the resistance and persistence of leukemic stem cells. To explore this phenomenon, we designed a single-cel...

journal_title：Experimental hematology

authors： Pagliaro S,Desterke C,Acloque H,Chomel JC,de Souza L,Hugues P,Griscelli F,Foudi A,Bennaceur-Griscelli A,Turhan AG

更新日期：2020-05-01 00:00:00

abstract：:Mds1-Evi1 (also known as Prdm3) and Prdm16 are two highly related zinc finger transcription factors that, within the hematopoietic system, are both expressed primarily in hematopoietic stem cells (HSCs). Our laboratory previously found that constitutive Mds1-Evi1 knockout mice are viable, but their HSCs are unable to ...

journal_title：Experimental hematology

authors： McGlynn KA,Sun R,Vonica A,Rudzinskas S,Zhang Y,Perkins AS

更新日期：2020-05-01 00:00:00

abstract：:Clonality studies can establish the single-cell origin of tumors and thus differentiate clonal malignant and premalignant processes from reactive polyclonal processes. Detection of clonal cells may be based on direct tracking of cell lineage-specific sequences or disease-specific somatic mutations identifying the clon...

journal_title：Experimental hematology

authors： Swierczek S,Prchal JT

更新日期：2020-03-01 00:00:00

abstract：:The history of clonal hematopoiesis (CH) research is punctuated by several seminal discoveries that have forged our understanding of cancer development. The clever application of the principle of random X-chromosome inactivation (XCI) in females led to the development of the first test to identify clonal derivation of...

journal_title：Experimental hematology

authors： Ayachi S,Buscarlet M,Busque L

更新日期：2020-03-01 00:00:00

abstract：:Cardiovascular diseases (CVDs) remain the leading cause of death worldwide. Many studies have provided evidence that both genetic and environmental factors induce atherosclerosis, leading thus to cardiovascular complications. Atherosclerosis is an inflammatory disease, and aging is strongly associated with the develop...

journal_title：Experimental hematology

authors： Pardali E,Dimmeler S,Zeiher AM,Rieger MA

更新日期：2020-03-01 00:00:00

abstract：:Serum albumin has long been an essential supplement for ex vivo hematopoietic and immune cell cultures. However, serum albumin medium supplements represent a major source of biological contamination in cell cultures and often cause loss of cellular function. As serum albumin exhibits significant batch-to-batch variabi...

journal_title：Experimental hematology

authors： Nishimura T,Hsu I,Martinez-Krams DC,Nakauchi Y,Majeti R,Yamazaki S,Nakauchi H,Wilkinson AC

更新日期：2019-12-01 00:00:00

abstract：:Megakaryocytes have been implicated in the micro-environmental abnormalities associated with fibrosis and hematopoietic failure in the bone marrow (BM) of primary myelofibrosis (PMF) patients, the Philadelphia-negative myeloproliferative neoplasm (MPN) associated with the poorest prognosis. To identify possible therap...

journal_title：Experimental hematology

authors： Ishikawa G,Fujiwara N,Hirschfield H,Varricchio L,Hoshida Y,Barosi G,Rosti V,Padilla M,Mazzarini M,Friedman SL,Hoffman R,Migliaccio AR

更新日期：2019-11-01 00:00:00

abstract：:The etiology of thrombocytopenia is important in treatment and management of the condition. Most platelet parameters that are routinely analyzed in the diagnostic laboratory have not proven useful in identifying the etiology, while specialized assays suffer from poor standardization and lack of agreement between labor...

journal_title：Experimental hematology

authors： Ali I,Graham C,Dempsey-Hibbert NC

更新日期：2019-10-01 00:00:00

abstract：:To investigate the value of genetic mutations in the pathogenesis and differential diagnosis of hemophagocytic lymphohistiocytosis (HLH), mutations related to blood and immune deficiency genes were analyzed in patients with HLH. Peripheral blood samples from 33 children diagnosed with HLH on the basis of the 2004 diag...

journal_title：Experimental hematology

authors： Mo W,Wei W,Sun Y,Yang Y,Guan Z,Li M,Zhu P,Chi Z

更新日期：2019-10-01 00:00:00

abstract：:Adult hematological malignancies, such as acute myeloid leukemia, are thought to arise through the gradual acquisition of oncogenic mutations within long-lived hematopoietic stem cells (HSCs). Genomic analysis of peripheral blood DNA has recently identified leukemia-associated genetic mutations within otherwise health...

journal_title：Experimental hematology

authors： Luis TC,Wilkinson AC,Beerman I,Jaiswal S,Shlush LI

更新日期：2019-09-01 00:00:00

abstract：:T(4;11) MLL-AF4 acute leukemia is one of the most aggressive malignancies in infant and pediatric populations. Epidemiological and functional studies have highlighted the influence of an overstimulation of the immune system on leukemia development. This study aimed at assessing if the cell-of-origin of t(4;11) MLL-AF4...

journal_title：Experimental hematology

authors： Malouf C,Ottersbach K

更新日期：2019-08-01 00:00:00

abstract：:Exosomes are virus-size membrane-bound vesicles of endocytic origin present in all body fluids. Plasma of AML patients is significantly enriched in exosomes, which carry a cargo of immunosuppressive molecules and deliver them to recipient immune cells, suppressing their functions. However, whether these exosomes origi...

journal_title：Experimental hematology

authors： Hong CS,Danet-Desnoyers G,Shan X,Sharma P,Whiteside TL,Boyiadzis M

更新日期：2019-08-01 00:00:00

abstract：:Histone deacetylase inhibitors (HDACIs) have demonstrated effectiveness against lymphomas and myelomas in clinical practice. However, common to all currently approved broad-acting HDACIs (panHDACIs) is dose-limiting thrombocytopenia, which has prevented wider use in cancer therapy. Using CD34+ hematopoietic stem cells...

journal_title：Experimental hematology

authors： Simic D,Sang N

更新日期：2019-04-01 00:00:00

abstract：:BCR-ABL-negative myeloproliferative neoplasms (MPNs) are driven by JAK-STAT pathway activation, but epigenetic alterations also play an important pathophysiological role. These can be pharmacologically manipulated with histone deacetylase inhibitors (HDACIs), which have proven to be clinically effective in the treatme...

journal_title：Experimental hematology

authors： Cardoso BA,Ramos TL,Belo H,Vilas-Boas F,Real C,Almeida AM

更新日期：2019-04-01 00:00:00

abstract：:More than 2million human erythroblasts extrude their nuclei every second in bone marrow under hypoxic conditions (<7% O2). Enucleation requires specific signal transduction pathways and the local assembly of contractile actomyosin rings. However, the energy source driving these events has not yet been identified. We e...

journal_title：Experimental hematology

authors： Goto T,Ubukawa K,Kobayashi I,Sugawara K,Asanuma K,Sasaki Y,Guo YM,Takahashi N,Sawada K,Wakui H,Nunomura W

更新日期：2019-04-01 00:00:00

abstract：:The ability to epigenetically reprogram differentiated somatic cells to pluripotency resulting in the discovery of induced pluripotent stem cells (iPSCs), has unlocked fundamental biologic insights into numerous genetic diseases. These insights have resulted from the key property of iPSCs to differentiate into all cel...

journal_title：Experimental hematology

authors： Chao MP,Majeti R

更新日期：2019-03-01 00:00:00

abstract：:Cell surface molecules aberrantly expressed or overexpressed by myeloid leukemic cells represent potential disease-specific therapeutic targets for antibodies. MUC1 is a polymorphic glycoprotein, the cleavage of which yields two unequal chains: a large extracellular α subunit containing a tandem repeat array bound in ...

journal_title：Experimental hematology

authors： Guillaume T,Dehame V,Chevallier P,Peterlin P,Garnier A,Grégoire M,Pichinuk E,Rubinstein DB,Wreschner DH

更新日期：2019-02-01 00:00:00

abstract：:After initially successful chemotherapy, relapse frequently jeopardizes the outcome of patients with acute leukemia. Because of their adverse characteristics of self-renewal and dormancy, leukemia stem cells have been hypothesized to play a critical role in resistance to antiproliferative chemotherapy and the developm...

journal_title：Experimental hematology

authors： Senft D,Jeremias I

更新日期：2019-01-01 00:00:00

abstract：:Benign constitutional neutropenia (BCN) is an asymptomatic condition characterized by mild chronic neutropenia in patients with no history of recurrent infections. Most patients are referred for further testing, increasing health care costs. We present an alternative form of assessment of individuals with BCN based on...

journal_title：Experimental hematology

authors： Souto Filho JTD,Portugal RD,Nucci M

更新日期：2019-01-01 00:00:00

abstract：:The transcription factor RUNX1 is required in the embryo for formation of the adult hematopoietic system. Here, we describe the seminal findings that led to the discovery of RUNX1 and of its critical role in blood cell formation in the embryo from hemogenic endothelium (HE). We also present RNA-sequencing data demonst...

journal_title：Experimental hematology

authors： Gao L,Tober J,Gao P,Chen C,Tan K,Speck NA

更新日期：2018-12-01 00:00:00

abstract：:The selection of chemotherapy regimen for elderly patients with acute myeloid leukemia (AML) remains challenging. Here, we report that granulocyte colony-stimulating factor (G-CSF) upregulates the expression of microRNA (miR)-146a in a nuclear factor kappaB-dependent manner, leading to direct decreases in the expressi...

journal_title：Experimental hematology

authors： Li X,Xu L,Sheng X,Cai J,Liu J,Yin T,Xiao F,Chen F,Zhong H

更新日期：2018-12-01 00:00:00

abstract：:The relationship between the hematopoietic stem cell (HSC) population and its surrounding bone marrow microenvironment is a rapidly evolving area of research. Normal HSC processes rely heavily on a complex communication network involving various marrow niches. Although leukemogenesis largely results from abnormal gene...

journal_title：Experimental hematology

authors： Miller LH,Qu CK,Pauly M

更新日期：2018-10-01 00:00:00

abstract：:Fanconi anemia (FA) is a rare recessive disease characterized by progressive bone marrow failure, congenital abnormalities, and increased incidence of cancers. To date, mutations in 22 genes can cause FA or an FA-like phenotype. In China, in addition to clinical information, FA diagnosis primarily relies on genetic se...

journal_title：Experimental hematology

authors： Li N,Ding L,Li B,Wang J,D'Andrea AD,Chen J

更新日期：2018-10-01 00:00:00

abstract：:Improving our understanding of the intricacies of hematopoietic specification of induced or embryonic human pluripotent stem cells is beneficial for many areas of research and translational medicine. Currently, it is not clear whether, during human pluripotent stem cells hematopoietic differentiation in vitro, the mat...

journal_title：Experimental hematology

authors： Galat Y,Elcheva I,Dambaeva S,Katukurundage D,Beaman K,Iannaccone PM,Galat V

更新日期：2018-09-01 00:00:00

abstract：:Haploinsufficiency of erythroid Krüppel-like factor (EKLF/KLF1) has been shown recently to ameliorate the clinical severity of β-thalassemia by increased expression levels of fetal hemoglobin (HbF). The underlying mechanisms for role of EKLF in regulating HbF are of great interest but remain incompletely understood. I...

journal_title：Experimental hematology

authors： Li Y,Liu D,Zhang X,Li Z,Ye Y,Liu Q,Shen J,Chen Z,Huang H,Liang Y,Han X,Liu J,An X,Mohandas N,Xu X

更新日期：2018-07-01 00:00:00

abstract：:The microenvironment (niche) governs the fate of stem cells (SCs) by balancing self-renewal and differentiation. Increasing evidence indicates that the tumor niche plays an active role in cancer, but its important properties for tumor initiation progression and resistance remain to be identified. Clinical data show th...

journal_title：Experimental hematology

authors： Zylbersztejn F,Flores-Violante M,Voeltzel T,Nicolini FE,Lefort S,Maguer-Satta V

更新日期：2018-05-01 00:00:00

abstract：:The aberrant overexpression of Wilms tumor-1 (WT1) in acute myeloid leukemia (AML) plays an important role in blast cell survival by enhancing proliferation and inhibiting apoptosis. However, the mechanism underlying the overexpression of WT1 remains unclear. Here, we identified miR-193a (miR-193a-5p) and miR-600 targ...

journal_title：Experimental hematology

authors： Li H,Xing C,Zhou B,Ye H,Feng J,Wu J,Gao S

更新日期：2018-05-01 00:00:00

abstract：:The advent of single-cell transcriptomics has led to the proposal of a number of novel high-resolution models for the hematopoietic system. Testing the predictions generated by such models requires cell fate potential assays of matching, single-cell resolution. Here we detail the development of an in vitro high-throug...

journal_title：Experimental hematology

authors： Khoramian Tusi B,Socolovsky M

更新日期：2018-04-01 00:00:00

abstract：:In adult hematopoiesis, the hematopoietic stem cell (HSC) sits at the top of a hierarchy of hematopoietic progenitors responsible for generating the diverse repertoire of blood and immune cells. During embryonic development, however, the initial waves of hematopoiesis provide the first functioning blood cells of the d...

journal_title：Experimental hematology

authors： Hadland B,Yoshimoto M

更新日期：2018-04-01 00:00:00

abstract：:Current next-generation sequencing (NGS) technologies allow unprecedented insights into the mutational profiles of tumors. Recent studies in myeloproliferative neoplasms have further demonstrated that, not only the mutational profile, but also the order in which these mutations are acquired is relevant for our underst...

journal_title：Experimental hematology

authors： Nice FL,Massie CE,Klampfl T,Green AR

更新日期：2018-01-01 00:00:00

abstract：:Septins are a family of filament-forming GTP-binding proteins that serve as scaffolds and diffusion barriers in various cellular processes. Septin 6 is known as a fusion partner of mixed-lineage leukemia in infant acute myeloid leukemia. The occurrence of the fusion gene is associated with a reduced expression of sept...

journal_title：Experimental hematology

authors： Senger K,Marka G,Soller K,Sakk V,Florian MC,Geiger H

更新日期：2017-11-01 00:00:00

abstract：:Myelodysplastic syndromes (MDSs) are clonal disorders of hematopoietic stem and progenitor cells and represent the most common cause of acquired marrow failure. Hallmarked by ineffective hematopoiesis, dysplastic marrow, and risk of transformation to acute leukemia, MDS remains a poorly treated disease. Although ident...

journal_title：Experimental hematology

authors： Li AJ,Calvi LM

更新日期：2017-11-01 00:00:00

abstract：:Acute myeloid leukemia (AML) develops when there is a block in differentiation and uncontrolled proliferation of myeloid precursors, resulting in bone marrow failure. AML is a clinically, morphologically, and genetically heterogeneous disease, and biological differences between adult and childhood AML have been identi...

journal_title：Experimental hematology

authors： Laing AA,Harrison CJ,Gibson BES,Keeshan K

更新日期：2017-10-01 00:00:00

abstract：:In hematopoietic cell development, the transcriptional program is strictly regulated in a lineage- and stage-specific manner that requires a number of transcription factors to work in a cascade or in a loop, in addition to interactions with nonhematopoietic cells in the microenvironment. Disruption of the transcriptio...

journal_title：Experimental hematology

authors： Sanda T,Leong WZ

更新日期：2017-09-01 00:00:00