当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > The G protein coupled receptor CXCR4 designed by the QTY code becomes more hydrophilic and retains cell signaling activity.

The G protein coupled receptor CXCR4 designed by the QTY code becomes more hydrophilic and retains cell signaling activity.

Abstract：

:G protein-coupled receptors (GPCRs) are vital for diverse biological functions, including vision, smell, and aging. They are involved in a wide range of diseases, and are among the most important targets of medicinal drugs. Tools that facilitate GPCR studies or GPCR-based technologies or therapies are thus critical to develop. Here we report using our QTY (glutamine, threonine, tyrosine) code to systematically replace 29 membrane-facing leucine, isoleucine, valine, and phenylalanine residues in the transmembrane α-helices of the GPCR CXCR4. This variant, CXCR4QTY29, became more hydrophilic, while retaining the ability to bind its ligand CXCL12. When transfected into HEK293 cells, it inserted into the cell membrane, and initiated cellular signaling. This QTY code has the potential to improve GPCR and membrane protein studies by making it possible to design functional hydrophilic receptors. This tool can be applied to diverse α-helical membrane proteins, and may aid in the development of other applications, including clinical therapies.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Tegler L,Corin K,Pick H,Brookes J,Skuhersky M,Vogel H,Zhang S

doi

10.1038/s41598-020-77659-x

subject

Has Abstract

pub_date

2020-12-07 00:00:00

pages

21371

issue

1

issn

2045-2322

pii

10.1038/s41598-020-77659-x

journal_volume

10

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • In situ characterization of protein aggregates in human tissues affected by light chain amyloidosis: a FTIR microspectroscopy study.

    abstract：:Light chain (AL) amyloidosis, caused by deposition of amyloidogenic immunoglobulin light chains (LCs), is the most common systemic form in industrialized countries. Still open questions, and premises for developing targeted therapies, concern the mechanisms of amyloid formation in vivo and the bases of organ targeting...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep29096

    authors： Ami D,Lavatelli F,Rognoni P,Palladini G,Raimondi S,Giorgetti S,Monti L,Doglia SM,Natalello A,Merlini G

    更新日期：2016-07-04 00:00:00

  • Differential effects of sodium chloride and monosodium glutamate on kidney of adult and aging mice.

    abstract：:Monosodium Glutamate (MSG) is used as flavour enhancer, with potential beneficial effects due to its nutritional value. Given the decline in kidney functions during aging, we investigated the impact of MSG voluntary intake on the kidney of male mice, aged 6 or 18 months. For 2 months, they freely consumed water (contr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-80048-z

    authors： Celestino M,Balmaceda Valdez V,Brun P,Castagliuolo I,Mucignat-Caretta C

    更新日期：2021-01-12 00:00:00

  • Simvastatin and Bezafibrate ameliorate Emotional disorder Induced by High fat diet in C57BL/6 mice.

    abstract：:High fat diet (HFD)-induced metabolic disorders may lead to emotional disorders. This study aimed to explore the effect of simvastatin (SMV) and bezafibrate (BZ) on improving HFD-induced emotional changes, and tried to identify their different mechanisms. The intraperitoneal glucose tolerance test (IPGTT) was used to ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-02576-5

    authors： Wang H,Zhou J,Liu QZ,Wang LL,Shang J

    更新日期：2017-05-24 00:00:00

  • What calls for service tell us about suicide: A 7-year spatio-temporal analysis of neighborhood correlates of suicide-related calls.

    abstract：:Previous research has shown that neighborhood-level variables such as social deprivation, social fragmentation or rurality are related to suicide risk, but most of these studies have been conducted in the U.S. or northern European countries. The aim of this study was to analyze the spatio-temporal distribution of suic...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-25268-0

    authors： Marco M,Gracia E,López-Quílez A,Lila M

    更新日期：2018-04-30 00:00:00

  • Phase Conjugated and Transparent Wavelength Conversions of Nyquist 16-QAM Signals Employing a Single-Layer Graphene Coated Fiber Device.

    abstract：:We fabricate a nonlinear optical device based on a fiber pigtail cross-section coated with a single-layer graphene grown by chemical vapor deposition (CVD) method. Using the fabricated graphene-assisted nonlinear optical device and employing Nyquist 16-ary quadrature amplitude modulation (16-QAM) signal, we experiment...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep22379

    authors： Hu X,Zeng M,Long Y,Liu J,Zhu Y,Zou K,Zhang F,Fu L,Wang J

    更新日期：2016-03-02 00:00:00

  • Communicative knowledge pervasively influences sensorimotor computations.

    abstract：:Referential pointing is a characteristically human behavior, which involves moving a finger through space to direct an addressee towards a desired mental state. Planning this type of action requires an interface between sensorimotor and conceptual abilities. A simple interface could supplement spatially-guided motor r...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-04442-w

    authors： Murillo Oosterwijk A,de Boer M,Stolk A,Hartmann F,Toni I,Verhagen L

    更新日期：2017-06-27 00:00:00

  • The effect of tillage systems on phosphorus distribution and forms in rhizosphere and non-rhizosphere soil under maize (Zea mays L.) in Northeast China.

    abstract：:An appropriate tillage method must be implemented by maize growers to improve phosphorus dynamics in the soil in order to increase phosphorus uptake by plant. The objective of this study was to investigate the effects of tillage systems on phosphorus and its fractions in rhizosphere and non-rhizosphere soils under mai...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-63567-7

    authors： Xomphoutheb T,Jiao S,Guo X,Mabagala FS,Sui B,Wang H,Zhao L,Zhao X

    更新日期：2020-04-20 00:00:00

  • Fine mapping of a leaf flattening gene Bralcm through BSR-Seq in Chinese cabbage (Brassica rapa L. ssp. pekinensis).

    abstract：:Leaf flattening influences plant photosynthesis, thereby affecting product yield and quality. Here, we obtained a stably inherited leaf crinkled mutant (lcm), derived from the Chinese cabbage doubled haploid (DH) 'FT' line using EMS mutagenesis combined with isolated microspore culture. The crinkled phenotype was cont...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-70975-2

    authors： Zhang M,Huang S,Gao Y,Fu W,Qu G,Zhao Y,Shi F,Liu Z,Feng H

    更新日期：2020-08-18 00:00:00

  • Health-related quality of life amongst people diagnosed with abdominal aortic aneurysm and peripheral artery disease and the effect of fenofibrate.

    abstract：:The aims of this study were, firstly, to assess the effect of concurrent peripheral artery disease (PAD) on the health-related quality of life (QOL) of people diagnosed with a small abdominal aortic aneurysm (AAA); and secondly, to test whether the peroxisome proliferator-activated receptor α agonist fenofibrate impro...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71454-4

    authors： Golledge J,Pinchbeck J,Rowbotham SE,Yip L,Jenkins JS,Quigley F,Moxon JV

    更新日期：2020-09-03 00:00:00

  • Closing the sea surface mixed layer temperature budget from in situ observations alone: Operation Advection during BoBBLE.

    abstract：:Sea surface temperature (SST) is a fundamental driver of tropical weather systems such as monsoon rainfall and tropical cyclones. However, understanding of the factors that control SST variability is lacking, especially during the monsoons when in situ observations are sparse. Here we use a ground-breaking observation...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-63320-0

    authors： Vijith V,Vinayachandran PN,Webber BGM,Matthews AJ,George JV,Kannaujia VK,Lotliker AA,Amol P

    更新日期：2020-04-27 00:00:00

  • Chiral Separation by Flows: The Role of Flow Symmetry and Dimensionality.

    abstract：:Separation of enantiomers by flows is a promising chiral resolution method using cost-effective microfluidics. Notwithstanding a number of experimental and numerical studies, a fundamental understanding still remains elusive, and an important question as to whether it is possible to specify common physical properties ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep35144

    authors： Ro S,Yi J,Kim YW

    更新日期：2016-10-14 00:00:00

  • Omega-3 fatty acids does not affect physical activity and body weight in primary school children - a double-blind randomized placebo-controlled trial.

    abstract：:It was hypothesized that supplementation of omega-3 fatty acids could increase physical activity (PA) levels, where traditional interventions often fail. The aim of this double-blind, randomized, placebo-controlled trail was to evaluate the effects of 15-week administration of omega-3 fatty acids on objectively measur...

    journal_title：Scientific reports

    pub_type： 杂志文章,随机对照试验

    doi：10.1038/s41598-018-31229-4

    authors： Svensson V,Johansson E,Fischer M,Deng SL,Hagströmer M,Danielsson P

    更新日期：2018-08-24 00:00:00

  • Tiling array study of MNNG treated Escherichia coli reveals a widespread transcriptional response.

    abstract：:The alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) is known to trigger the adaptive response by inducing the ada-regulon - consisting of three DNA repair enzymes Ada, AlkB, AlkA and the enigmatic AidB. We have applied custom designed tiling arrays to study transcriptional changes in Escherichia coli foll...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep03053

    authors： Booth JA,Thomassen GO,Rowe AD,Weel-Sneve R,Lagesen K,Kristiansen KI,Bjørås M,Rognes T,Lindvall JM

    更新日期：2013-10-25 00:00:00

  • Growth disorders in children and adolescents affected by syndromes or diseases associated with neurodysfunction.

    abstract：:We have observed that one in three patients admitted to the Neurological Rehabilitation Ward for Children and Adolescents due to a syndrome or disease associated with neurodysfunction is short of stature for their age. In order to identify the relationship between growth defects (short stature) and syndromes or diseas...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-52918-8

    authors： Perenc L,Guzik A,Podgórska-Bednarz J,Drużbicki M

    更新日期：2019-11-11 00:00:00

  • Expansion of different subpopulations of CD26-/low T cells in allergic and non-allergic asthmatics.

    abstract：:CD26 displays variable levels between effector (TH17 ≫ TH1 > TH2 > Treg) and naïve/memory (memory > naïve) CD4+ T lymphocytes. Besides, IL-6/IL-6R is associated with TH17-differentiation and asthma severity. Allergic/atopic asthma (AA) is dominated by TH2 responses, while TH17 immunity might either modulate the TH2-de...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43622-8

    authors： Nieto-Fontarigo JJ,Salgado FJ,San-José ME,Cruz MJ,Valdés L,Pérez-Díaz A,Arias P,Nogueira M,González-Barcala FJ

    更新日期：2019-05-17 00:00:00

  • Proteomics Profiling Reveals Carbohydrate Metabolic Enzymes and 14-3-3 Proteins Play Important Roles for Starch Accumulation during Cassava Root Tuberization.

    abstract：:Cassava is one of the most important root crops as a reliable source of food and carbohydrates. Carbohydrate metabolism and starch accumulation in cassava storage root is a cascade process that includes large amounts of proteins and cofactors. Here, comparative proteomics were conducted in cassava root at nine develop...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep19643

    authors： Wang X,Chang L,Tong Z,Wang D,Yin Q,Wang D,Jin X,Yang Q,Wang L,Sun Y,Huang Q,Guo A,Peng M

    更新日期：2016-01-21 00:00:00

  • Implications of C1q/TNF-related protein superfamily in patients with coronary artery disease.

    abstract：:The C1q complement/TNF-related protein superfamily (CTRPs) displays differential effects on the regulation of metabolic homeostasis, governing cardiovascular function. However, whether and how they may serve as predictor/pro-diagnosis factors for assessing the risks of coronary artery disease (CAD) remains controversi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-57877-z

    authors： Zhang Y,Liu C,Liu J,Guo R,Yan Z,Liu W,Lau WB,Jiao X,Cao J,Xu K,Jia Y,Ma X,Wang Y

    更新日期：2020-01-21 00:00:00

  • Effects of maternal obesity on Wharton's Jelly mesenchymal stromal cells.

    abstract：:We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord's Wharton's Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-18034-1

    authors： Badraiq H,Cvoro A,Galleu A,Simon M,Miere C,Hobbs C,Schulz R,Siow R,Dazzi F,Ilic D

    更新日期：2017-12-14 00:00:00

  • TSA restores hair follicle-inductive capacity of skin-derived precursors.

    abstract：:The genesis of the hair follicle relies on signals derived from mesenchymal cells in the dermis during skin morphogenesis and regeneration. Multipotent skin-derived precursors (SKPs), which exhibit long term proliferation potential when being cultured in spheroids, have been shown to induce hair genesis and hair folli...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-39394-w

    authors： Guo L,Wang X,Yuan J,Zhu M,Fu X,Xu RH,Wu C,Wu Y

    更新日期：2019-02-27 00:00:00

  • A database of breast oncogenic specific siRNAs.

    abstract：:Breast cancer is a serious problem causing the death of women across the world. At present, one of the major challenges is to design drugs to target breast cancer specific gene(s). RNA interference (RNAi) is an important technique for targeted gene silencing that may lead to promising novel therapeutic strategies for ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-08948-1

    authors： Tyagi A,Semwal M,Sharma A

    更新日期：2017-08-18 00:00:00

  • Imaging local brain activity of multiple freely moving mice sharing the same environment.

    abstract：:Electrophysiological field potential dynamics have been widely used to investigate brain functions and related psychiatric disorders. Considering recent demand for its applicability to freely moving subjects, especially for animals in a group and socially interacting with each other, here we propose a new method based...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43897-x

    authors： Inagaki S,Agetsuma M,Ohara S,Iijima T,Yokota H,Wazawa T,Arai Y,Nagai T

    更新日期：2019-05-16 00:00:00

  • Structure- and conformation-activity studies of nociceptin/orphanin FQ receptor dimeric ligands.

    abstract：:The peptide nociceptin/orphanin FQ (N/OFQ) and the N/OFQ receptor (NOP) constitute a neuropeptidergic system that modulates various biological functions and is currently targeted for the generation of innovative drugs. In the present study dimeric NOP receptor ligands with spacers of different lengths were generated u...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep45817

    authors： Pacifico S,Carotenuto A,Brancaccio D,Novellino E,Marzola E,Ferrari F,Cerlesi MC,Trapella C,Preti D,Salvadori S,Calò G,Guerrini R

    更新日期：2017-04-06 00:00:00

  • Absence Seizure Control by a Brain Computer Interface.

    abstract：:The ultimate goal of epileptology is the complete abolishment of epileptic seizures. This might be achieved by a system that predicts seizure onset combined with a system that interferes with the process that leads to the onset of a seizure. Seizure prediction remains, as of yet, unresolved in absence-epilepsy, due to...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-02626-y

    authors： Maksimenko VA,van Heukelum S,Makarov VV,Kelderhuis J,Lüttjohann A,Koronovskii AA,Hramov AE,van Luijtelaar G

    更新日期：2017-05-29 00:00:00

  • Discovery of Amorphous Iron Hydrides via Novel Quiescent Reaction in Aqueous Solution.

    abstract：:Novel amorphous iron hydrides (AIHs) are synthesized for the first time under ambient conditions by employing novel "quiescent reaction", without stirring for mixing solutions, during a conventional aqueous reduction-precipitation process. The kind and morphology of AIHs are dependent on the processing condition, wher...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-63124-2

    authors： Taguchi K,Shinozaki K,Okumura H,Michioka C,Yoshimura K,Ishihara KN

    更新日期：2020-04-10 00:00:00

  • Nicotinamide mononucleotide (NMN) supplementation ameliorates the impact of maternal obesity in mice: comparison with exercise.

    abstract：:Maternal overnutrition increases the risk of long-term metabolic dysfunction in offspring. Exercise improves metabolism partly by upregulating mitochondrial biogenesis or function, via increased levels of nicotinamide adenine dinucleotide (NAD+). We have shown that the NAD+ precursor, nicotinamide mononucleotide (NMN)...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-14866-z

    authors： Uddin GM,Youngson NA,Doyle BM,Sinclair DA,Morris MJ

    更新日期：2017-11-08 00:00:00

  • Strongly Confined Spoof Surface Plasmon Polaritons Waveguiding Enabled by Planar Staggered Plasmonic Waveguides.

    abstract：:We demonstrate a novel route to achieving highly efficient and strongly confined spoof surface plasmon polaritons (SPPs) waveguides at subwavelength scale enabled by planar staggered plasmonic waveguides (PSPWs). The structure of these new waveguides consists of an ultrathin metallic strip with periodic subwavelength ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38528

    authors： Ye L,Xiao Y,Liu Y,Zhang L,Cai G,Liu QH

    更新日期：2016-12-05 00:00:00

  • Reversible Underwater Adhesion: The Unique C-shaped Suckers of Net-winged Midge Larvae (Blepharicera sp.).

    abstract：:Aquatic insects living in fast-flowing streams have developed various types of attachment systems to resist being carried away by strong currents. Combinations of various attachment devices offer aquatic insects advantages in underwater adhesion on substrates with different surface properties. In this study, the net-w...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66268-3

    authors： Liu GL,Chang HK,Chuang YC,Lin YM,Chen PY

    更新日期：2020-06-10 00:00:00

  • A biomolecular anthropological investigation of William Adams, the first SAMURAI from England.

    abstract：:William Adams (Miura Anjin) was an English navigator who sailed with a Dutch trading fleet to the far East and landed in Japan in 1600. He became a vassal under the Shogun, Tokugawa Ieyasu, was bestowed with a title, lands and swords, and became the first SAMURAI from England. "Miura" comes from the name of the territ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-78723-2

    authors： Mizuno F,Ishiya K,Matsushita M,Matsushita T,Hampson K,Hayashi M,Tokanai F,Kurosaki K,Ueda S

    更新日期：2020-12-10 00:00:00

  • Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat.

    abstract：:Plants deploy several ammonium transporter (AMT) and nitrate transporter (NRT) genes to acquire NH4+ and NO3- from the soil into the roots and then transport them to other plant organs. Coding sequences of wheat genes obtained from ENSEMBL were aligned to known AMT and NRT sequences of Arabidopsis, barley, maize, rice...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-25430-8

    authors： Bajgain P,Russell B,Mohammadi M

    更新日期：2018-05-04 00:00:00

  • Intraluminal pressure patterns in the human colon assessed by high-resolution manometry.

    abstract：:Assessment of colonic motor dysfunction is rarely done because of inadequate methodology and lack of knowledge about normal motor patterns. Here we report on elucidation of intraluminal pressure patterns using High Resolution Colonic Manometry during a baseline period and in response to a meal, in 15 patients with con...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep41436

    authors： Chen JH,Yu Y,Yang Z,Yu WZ,Chen WL,Yu H,Kim MJ,Huang M,Tan S,Luo H,Chen J,Chen JD,Huizinga JD

    更新日期：2017-02-20 00:00:00