Abstract:
:Fumarylacetoacetate hydrolase (FAH) is the last enzyme in the degradation pathway of the amino acids tyrosine and phenylalanine in mammals that catalyzes the hydrolysis of 4-fumarylacetoacetate into acetoacetate and fumarate. Mutations of the FAH gene are associated with hereditary tyrosinemia type I (HT1), resulting in reduced protein stability, misfolding, accelerated degradation and deficiency in functional proteins. Identifying E3 ligases, which are necessary for FAH protein stability and degradation, is essential. In this study, we demonstrated that the FAH protein level is elevated in liver cancer tissues compared to that in normal tissues. Further, we showed that the FAH protein undergoes 26S proteasomal degradation and its protein turnover is regulated by the anaphase-promoting complex/cyclosome-Cdh1 (APC/C)Cdh1 E3 ubiquitin ligase complex. APC/CCdh1 acts as a negative stabilizer of FAH protein by promoting FAH polyubiquitination and decreases the half-life of FAH protein. Thus, we envision that Cdh1 might be a key factor in the maintenance of FAH protein level to regulate FAH-mediated physiological functions.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Kaushal K,Woo SH,Tyagi A,Kim DH,Suresh B,Kim KS,Ramakrishna Sdoi
10.3390/ijms21228719subject
Has Abstractpub_date
2020-11-18 00:00:00issue
22issn
1422-0067pii
ijms21228719journal_volume
21pub_type
杂志文章abstract::Overdose of acetaminophen (APAP), an antipyretic drug, is an important cause of liver injury. However, the mechanism in the rat model remains undetermined. We analyzed APAP-induced hepatotoxicity using rats based on M1/M2-macrophage functions in relation to damage-associated molecular patterns (DAMPs) and autophagy. L...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21238998
更新日期:2020-11-26 00:00:00
abstract::Atrazine, a member of the 2-chloro-s-triazine family of herbicides, is the most widely used pesticide in the world and often detected in agriculture watersheds. Although it was generally considered as an endocrine disruptor, posing a potential threat to human health, the molecular mechanisms of atrazine effects remain...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151017806
更新日期:2014-10-01 00:00:00
abstract::Chitosan-caseinate nanoparticles were synthesized by polyelectrolyte complex (PEC) formation. Caseinate is an anionic micellar nanocolloid in aqueous solutions, which association with the polycationic chitosan yielded polyelectrolyte complexes with caseinate cores surrounded by a chitosan corona. The pre-structuration...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165602
更新日期:2020-08-05 00:00:00
abstract::Alzheimer's disease (AD) is the leading cause of dementia worldwide. It involves progressive impairment of cognitive function. A growing number of neuroprotective compounds have been identified with potential anti-AD properties through in vitro and in vivo models of AD. Quercetin, a natural flavonoid contained in a wi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21020493
更新日期:2020-01-13 00:00:00
abstract::Sortase A (SrtA) is a membrane-associated enzyme that anchors surface-exposed proteins to the cell wall envelope of Gram-positive bacteria such as Staphylococcus aureus. As SrtA is essential for Gram-positive bacterial pathogenesis but dispensable for microbial growth or viability, SrtA is considered a favorable targe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21228601
更新日期:2020-11-14 00:00:00
abstract::A dodecadepsipeptide valinomycin (VLM) has been most recently reported to be a potential anti-coronavirus drug that could be efficiently produced on a large scale. It is thus of importance to study solid-phase forms of VLM in order to be able to ensure its polymorphic purity in drug formulations. The previously availa...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21144907
更新日期:2020-07-11 00:00:00
abstract::The transmembrane K+/H+ antiporters of NhaP type of Vibrio cholerae (Vc-NhaP1, 2, and 3) are critical for maintenance of K+ homeostasis in the cytoplasm. The entire functional NhaP group is indispensable for the survival of V. cholerae at low pHs suggesting their possible role in the acid tolerance response (ATR) of V...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20102572
更新日期:2019-05-25 00:00:00
abstract::Henrin A (1), a new ent-kaurane diterpene, was isolated from the leaves of Pteris henryi. The chemical structure was elucidated by analysis of the spectroscopic data including one-dimensional (1D) and two-dimensional (2D) NMR spectra, and was further confirmed by X-ray crystallographic analysis. The compound was evalu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161126071
更新日期:2015-11-24 00:00:00
abstract::Non-coding RNA species contribute more than 90% of all transcripts and have gained increasing attention in the last decade. One of the most recent members of this group are long non-coding RNAs (lncRNAs) which are characterized by a length of more than 200 nucleotides and a lack of coding potential. However, in contra...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20133276
更新日期:2019-07-03 00:00:00
abstract::Ceramide and diacylglycerol are linked to insulin resistance in rodents, but in humans the data are inconsistent. Insulin resistance is frequently observed with aging, but the role of ceramide and diacylglycerol is not clarified. Training improves metabolic health and, therefore, we aimed to elucidate the influence of...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20051240
更新日期:2019-03-12 00:00:00
abstract::The only curative treatment for hepatic failure is liver transplantation. Unfortunately, this treatment has several major limitations, as for example donor organ shortage. A previous report demonstrated that transplantation of induced pluripotent stem cells without reprogramming factor c-Myc (3-genes iPSCs) attenuates...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13033598
更新日期:2012-01-01 00:00:00
abstract::The composition of IL-23R complex is similar to that of the IL-12 receptor (IL-12R) complex with a shared IL-12R-β1 chain. The IL-12R-β1 heterodimerizes with IL-23R and IL-12R-β2 to form IL-23R and IL-12R complexes, respectively. The IL-12R-β2 has been shown to function as a tumor suppressor gene and apoptotic inducer...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms141224656
更新日期:2013-12-18 00:00:00
abstract::Alzheimer's disease (AD) is characterized by extracellular accumulation of amyloid-β peptide (Aβ), generated by proteolytic processing of the amyloid precursor protein (APP) by β- and γ-secretase. Aβ generation is inhibited when the initial ectodomain shedding is caused by α-secretase, cleaving APP within the Aβ domai...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14035879
更新日期:2013-03-13 00:00:00
abstract::The regeneration of neurons is an important goal of neuroscience and clinical medicine. The electrical stimulation of cells is a promising technique to meet this goal. However, its efficiency highly depends on the electrochemical properties of the stimulation electrodes used. This work reports on the preparation and u...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20082013
更新日期:2019-04-24 00:00:00
abstract::Most pharmaceutical substances interact with several or even many molecular targets in the organism, determining the complex profiles of their biological activity. Moreover, due to biotransformation in the human body, they form one or several metabolites with different biological activity profiles. Therefore, the deve...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207492
更新日期:2020-10-11 00:00:00
abstract::Nimodipine is well characterized for the management of aneurysmal subarachnoid hemorrhage and has been shown to promote a better outcome and less delayed ischemic neurological deficits. Animal and clinical trials show neuroprotective efficacy following nerve injuries. We showed a neuroprotective effect on Neuro2a cell...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18050964
更新日期:2017-05-03 00:00:00
abstract::Biologically active chemical compounds may provide remedies for several diseases. Meanwhile, Machine Learning techniques applied to Drug Discovery, which are cheaper and faster than wet-lab experiments, have the capability to more effectively identify molecules with the expected pharmacological activity. Therefore, it...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20092175
更新日期:2019-05-02 00:00:00
abstract::We have tested the hypothesis that neuropathic pain acting as a stressor drives functional plasticity in the sympathoadrenal system. The relation between neuropathic pain and adrenal medulla function was studied with behavioral, immunohistochemical and electrophysiological techniques in rats subjected to chronic const...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20010155
更新日期:2019-01-03 00:00:00
abstract::Maurizio Battino, Ph.D., Associate Professor of Biochemistry in the Department of Clinical Sciences, Faculty of Medicine, Università Politecnica delle Marche (Italy), is the Director of the Centre for Health and Nutrition, Universidad Europea del Atlantico (Santander, Spain) and Director of Nutrition and Health projec...
journal_title:International journal of molecular sciences
pub_type: 面试
doi:10.3390/ijms17071038
更新日期:2016-07-05 00:00:00
abstract::Polysaccharides from Morchella esculenta have been proven to be functional and helpful for humans. The purpose of this study was to investigate the chemical structure and anti-proliferating and antitumor activities of a Morchella esculenta polysaccharide (MEP) extracted by pulsed electric field (PEF) in submerged ferm...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17060986
更新日期:2016-06-22 00:00:00
abstract::A new anticancer ligustrazine derivative, 3β-hydroxyolea-12-en-28-oic acid- 3,5,6-trimethylpyrazin-2-methylester (T-OA, C38H58O3N2), was previously reported. It was synthesized via conjugating hepatoprotective and anticancer ingredients of traditional Chinese medicine. We found that T-OA exerted its anticancer activit...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160716401
更新日期:2015-07-17 00:00:00
abstract::Although higher nuclear factor κB (NFκB) expression and activity is observed in preeclamptic placentas, its mechanism of activation is unknown. This is the first study to investigate whether the canonical, non-canonical, or atypical NFκB activation pathways may be responsible for the higher activation of NFκB observed...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21155574
更新日期:2020-08-04 00:00:00
abstract::The worldwide prevalence of neurological and neurodegenerative disorders, such as depression or Alzheimer's disease, has spread extensively throughout the last decades, becoming an enormous health issue. Numerous data indicate a distinct correlation between the altered endocannabinoid signaling and different aspects o...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21124221
更新日期:2020-06-13 00:00:00
abstract::Poly(l-lactide-co-glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and bone tissue regeneration potential. The scaffolds were fabricated using solvent casting/particulate leachin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207541
更新日期:2020-10-13 00:00:00
abstract::Dysregulation of platelet function can contribute to the disease progression in sepsis. The proteasome represents a critical and vital element of cellular protein metabolism in platelets and its proteolytic activity has been associated with platelet function. However, the role of the platelet proteasome as well as its...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20235961
更新日期:2019-11-27 00:00:00
abstract::Thyroid cancer incidence has increased worldwide; however, investigations of thyroid cancer-related factors as potential prognosis markers remain insufficient. Secreted proteins from the cancer secretome are regulators of several molecular mechanisms and are, thereby, ideal candidates for potential markers. We aimed t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249537
更新日期:2020-12-15 00:00:00
abstract::Receptor targeted nuclear imaging directed against molecular markers overexpressed on breast cancer (BC) cells offers a sensitive and specific method for BC imaging. Currently, a few targets such as estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2), somatostatin recept...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18020260
更新日期:2017-01-26 00:00:00
abstract::Prostate cancer (PrCa) ranks among the top five cancers for both incidence and mortality worldwide. A significant proportion of PrCa susceptibility has been attributed to inherited predisposition, with 10-20% of cases expected to occur in a hereditary/familial context. Advances in DNA sequencing technologies have unco...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21145036
更新日期:2020-07-16 00:00:00
abstract::Vascular endothelial growth factor A (Vegfa) signaling regulates cardiovascular development. However, the cellular mechanisms of Vegfa signaling in early cardiogenesis remain poorly understood. The present study aimed to understand the differential functions and mechanisms of Vegfa signaling in cardiac development. A ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18020444
更新日期:2017-02-21 00:00:00
abstract::In wheat, flag leaf, stem, chaff and awns contribute to grain filling through photosynthesis and/or re-mobilization. Environmental and genetic effects on the relative contribution of each organ were examined by analyzing the consequences of sink-source manipulations (shading and excision) and by comparing carbon isoto...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19010056
更新日期:2017-12-25 00:00:00