Abstract:
:Nimodipine is well characterized for the management of aneurysmal subarachnoid hemorrhage and has been shown to promote a better outcome and less delayed ischemic neurological deficits. Animal and clinical trials show neuroprotective efficacy following nerve injuries. We showed a neuroprotective effect on Neuro2a cells. Subsequent microarray analysis revealed-among others-fatty acid 2-hydroxylase (FA2H) upregulated by nimodipine in vitro, which is a component of myelin synthesis. Differentiated Neuro2a cells were analyzed for nimodipine-mediated survival considering stress treatment in comparison to nifedipine-treatment. Cell survival was determined by measurement of LDH activity in the culture medium. Nimodipine decreased surgery-like stress-induced cell death of differentiated Neuro2a cells. Neuro2a cell culture was analyzed for changes in FA2H expression induced by nimodipine or nifedipine in surgery-like stress conditions. We analyzed expression levels of FA2H mRNA and protein by qPCR using fa2h specific primers or a FA2H-specific antibody in nimodipine or nifedipine non- and pre-treated Neuro2a cell culture, respectively. Nimodipine but not nifedipine increases FA2H protein levels and also significantly increases mRNA levels of FA2H in both undifferentiated and differentiated Neuro2a cells. Our findings indicate that higher expression of FA2H induced by nimodipine may cause higher survival of Neuro2a cells stressed with surgery-like stressors.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Herzfeld E,Speh L,Strauss C,Scheller Cdoi
10.3390/ijms18050964subject
Has Abstractpub_date
2017-05-03 00:00:00issue
5issn
1422-0067pii
ijms18050964journal_volume
18pub_type
杂志文章abstract::Tauopathy is a class of a neurodegenerative disorder linked with tau hyperphosphorylation, proteolysis, and aggregation. Tau can be subjected to proteolysis upon calpain activation in Alzheimer disease (AD), and traumatic brain injury (TBI). We and others have extensively researched calpain-mediated tau breakdown prod...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205213
更新日期:2019-10-21 00:00:00
abstract::Mechanical unloading simultaneously induces muscle and bone loss, but its mechanisms are not fully understood. The interactions between skeletal muscle and bone have been recently noted. Although canonical wingless-related integration site (Wnt)/β-catenin signaling is crucial for bone metabolism, its roles in the musc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21072547
更新日期:2020-04-06 00:00:00
abstract::Although not a disease itself, aging represents a risk factor for many aging-related illnesses, including cancer. Numerous causes underlie the increased incidence of malignancies in the elderly, for example, genomic instability and epigenetic alterations that occur at cellular level, which also involve the immune cell...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19123774
更新日期:2018-11-27 00:00:00
abstract::It has been confirmed that alternatively activated macrophages (M2) participate in tissue remodeling and fibrosis occurrence, but the effect of M2 on peritoneal fibrosis related to peritoneal dialysis (PD) hasn't been elucidated. This study was therefore conducted to assess the association between M2 and peritoneal fi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140510369
更新日期:2013-05-17 00:00:00
abstract::In many social anxiety disorder (SAD) patients, the efficacy of antidepressant therapy is unsatisfactory. Here, we investigated whether mice deficient for the lysosomal glycoprotein acid sphingomyelinase (ASM-/-) represent an appropriate tool to study antidepressant-resistant social fear. We also investigated whether ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218220
更新日期:2020-11-03 00:00:00
abstract::Differentiation and variation in body color has been a growing limitation to the commercial value of red tilapia. Limited microRNA (miRNA) information is available on skin color differentiation and variation in fish so far. In this study, a high-throughput Illumina sequencing of sRNAs was conducted on three color vari...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19041209
更新日期:2018-04-16 00:00:00
abstract::Before utilization in biomedical diagnosis, therapeutic treatment, and biotechnology, the diverse variety of peptides and proteins must be preliminarily purified and thoroughly characterized. The recombinant DNA technology and heterologous protein expression have helped simplify the isolation of targeted polypeptides ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21030928
更新日期:2020-01-31 00:00:00
abstract::The homeostatic control of lipid metabolism is essential for many fundamental physiological processes. A deep understanding of its regulatory mechanisms is pivotal to unravel prospective physiopathological factors and to identify novel molecular targets that could be employed to design promising therapies in the manag...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041297
更新日期:2020-02-14 00:00:00
abstract::MTH1 (MutT homolog 1) or NUDT1 (Nudix Hydrolase 1), also known as oxidized purine nucleoside triphosphatase, has potential as a biomarker for monitoring cancer progression and quantifying target engagement for relevant therapies. In this study, we validate one MTH1 inhibitor TH287 as a PET MTH1 radiotracer. TH287 was ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21228860
更新日期:2020-11-23 00:00:00
abstract::Seed storability, defined as the ability to remain alive during storage, is an important agronomic and physiological characteristic, but the underlying genetic mechanism remains largely unclear. Here, we report quantitative trait loci (QTLs) analyses for seed storability using a high-density single nucleotide polymorp...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20184442
更新日期:2019-09-09 00:00:00
abstract::RNAi technology has significant potential as a future therapeutic and could theoretically be used to knock down disease-specific RNAs. However, due to frequent off-target effects, low efficiency, and limited accessibility of nuclear transcripts, the clinical application of the technology remains challenging. In this s...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21238904
更新日期:2020-11-24 00:00:00
abstract::Preimplantation horse conceptuses require nutrients and signals from histotroph, the composition of which is regulated by luteal progesterone and conceptus-secreted factors. To distinguish progesterone and conceptus effects we shortened the period of endometrial progesterone-priming by asynchronous embryo transfer. Da...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21072562
更新日期:2020-04-07 00:00:00
abstract::The present study was designed to probe the effects of Huperzine A (HupA) on diabetes-associated cognitive decline (DACD) using a streptozotocin (STZ)-injected rat model. Diabetic rats were treated with HupA (0.05 and 0.1 mg/kg) for seven weeks. Memory functions were evaluated by the water maze test. Nissl staining wa...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15057667
更新日期:2014-05-05 00:00:00
abstract::Cystic fibrosis is an inherited multi-organ disorder caused by mutations in the CFTR gene. Patients with this disease exhibit characteristic abnormalities in the levels of unsaturated fatty acids in blood and tissue. Recent studies have uncovered an underlying biochemical mechanism for some of these changes, namely in...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms150916083
更新日期:2014-09-11 00:00:00
abstract::Amino acid transporters alanine-serine-cysteine transporter 2 (ASCT2) and L-Type Amino Acid Transporter 1 (LAT1) are coordinately enhanced in human cancers where among other roles, they are thought to drive mechanistic target-of-rapamycin (mTOR) growth signaling. To assess ASCT2 and LAT1 as therapeutic targets, nine u...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19072093
更新日期:2018-07-19 00:00:00
abstract::Oral biofilms attach onto both teeth surfaces and dental material surfaces in oral cavities. In the meantime, oral biofilms are not only the pathogenesis of dental caries and periodontitis, but also secondary caries and peri-implantitis, which would lead to the failure of clinical treatments. The material surfaces exp...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19103157
更新日期:2018-10-14 00:00:00
abstract::MicroRNAs (miRNAs) are a major class of small, noncoding RNA molecules that regulate gene expression by targeting mRNAs to trigger either translational repression or mRNA degradation. They have recently been more widely investigated due to their potential role as targets for cancer therapy. Many miRNAs have been impli...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms131013414
更新日期:2012-10-18 00:00:00
abstract::Amyloid A (AA) amyloidosis is a condition in which amyloid fibrils characterized by a linear morphology and a cross-β structure accumulate and are deposited extracellularly in organs, resulting in chronic inflammatory diseases and infections. The incidence of AA amyloidosis is high in humans and several animal species...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22031036
更新日期:2021-01-21 00:00:00
abstract::Ramie (Boehmeria nivea L. Gaud), commonly known as China grass, is a perennial bast fiber plant of the Urticaceae. In China, ramie farming, industry, and trade provide income for about five million people. Drought stress severely affects ramie stem growth and causes a dramatic decrease in ramie fiber production. There...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16023493
更新日期:2015-02-04 00:00:00
abstract::O6-alkylguanine-DNA alkyltransferase (AGT) is the main cause of tumor cell resistance to DNA-alkylating agents, so it is valuable to design tumor-targeted AGT inhibitors with hypoxia activation. Based on the existing benchmark inhibitor O6-benzylguanine (O6-BG), four derivatives with hypoxia-reduced potential and thei...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20246308
更新日期:2019-12-13 00:00:00
abstract::This study aimed to examine whether inhibition of hexokinase (HK)-II activity enhances the efficacy of sorafenib in in-vivo models of hepatocellular carcinoma (HCC), and to evaluate the prognostic implication of HK-II expression in patients with HCC. We used 3-bromopyruvate (3-BP), a HK-II inhibitor to target HK-II. T...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20061292
更新日期:2019-03-14 00:00:00
abstract::Metastatic castration-resistant prostate cancer is commonly treated with chemotherapy, whose effect is less than satisfactory. This raised the need for novel agents for the treatment of prostate cancer. In the present study, five phthalimide-based curcumin derivatives were synthesized and completely characterized to a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20010028
更新日期:2018-12-21 00:00:00
abstract::Aerobic metabolism occurs in a background of oxygen radicals and reactive oxygen species (ROS) that originate from the incomplete reduction of molecular oxygen in electron transfer reactions. The essential role of aerobic metabolism, the generation and consumption of ATP and other high energy phosphates, sustains a ba...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms12106469
更新日期:2011-01-01 00:00:00
abstract::One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to catalog and annotate a myriad of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve a detailed and personalized understanding into proteomes, we suggest using a customized RNA-seq library of potential proteoform...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21113813
更新日期:2020-05-27 00:00:00
abstract::Plant NADPH oxidases, formerly known as respiratory burst oxidase homologues (RBOHs), are plasma membrane enzymes dedicated to reactive oxygen species (ROS) production. These oxidases are implicated in a wide variety of processes, ranging from tissue and organ growth and development to signaling pathways in response t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17050680
更新日期:2016-05-18 00:00:00
abstract::The insect midgut secretes a semi-permeable, acellular peritrophic membrane (PM) that maintains intestinal structure, promotes digestion, and protects the midgut from food particles and pathogenic microorganisms. Peritrophin is an important PM protein (PMP) in the PM. Here, we identified 11 peritrophins with 1-16 chit...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21217973
更新日期:2020-10-27 00:00:00
abstract::Persistent inflammation is a complication associated with many ocular diseases. Changes in ocular vessels can amplify disease responses and contribute to vision loss by influencing the delivery of leukocytes to the eye, vascular leakage, and perfusion. Here, we report the anti-inflammatory activity for AXT107, a non-R...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21145142
更新日期:2020-07-21 00:00:00
abstract::Molecular factors involved in neuroprotection are key in the design of novel multitarget drugs in aging and neurodegeneration. AVCRI104P3 is a huprine derivative that exhibits potent inhibitory effects on human AChE, BuChE, and BACE-1 activities, as well as on AChE-induced and self-induced Aβ aggregation. More recentl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19092615
更新日期:2018-09-04 00:00:00
abstract::Vasoactive intestinal peptide receptor 1 (VPAC1) is a member of a secretin-like subfamily of G protein-coupled receptors. Its endogenous neuropeptide (VIP), secreted by neurons and immune cells, modulates various physiological functions such as exocrine and endocrine secretions, immune response, smooth muscles relaxat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20184348
更新日期:2019-09-05 00:00:00
abstract::Verticillium dahliae (V. dahliae) infects roots and colonizes the vascular vessels of host plants, significantly reducing the economic yield of cotton and other crops. In this study, the protein VdTHI20, which is involved in the thiamine biosynthesis pathway, was characterized by knocking out the corresponding VdTHI20...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041378
更新日期:2020-02-18 00:00:00