Abstract：
:We propose a visual strategy for simultaneous detection of multiple adulterated components in beef by integration of multiple polymerase chain reaction (mPCR) with the lateral flow strip (LFS). The primer sets for adulterated components are uniquely designed with different nucleic acid tags (NAT), enabling the amplicons with specific wobbled sequences at two opposite ends. The wobbled sequences will precisely hybridize with the pre-immobilized capture probes on T lines (T1, T2 and T3) and C line, contributing to the coloration of LFS. Taking advantages of extraordinary amplification efficiency of PCR and simplicity of LFS, common adulterated components including chicken, duck and pork can be easily detected with LOD as low as 0.01% (wt%), which is comparable to that of quantitative real-time polymerase chain reaction (qPCR) but with more simplified operations and reduced costs. The method can be extended to identification of other components by replacing the functional primer set. This method can be a useful candidate for meat quality control at the resource-limited setups.
journal_name
Food Chemjournal_title
Food chemistryauthors
Qin P,Xu J,Yao L,Wu Q,Yan C,Lu J,Yao B,Liu G,Chen Wdoi
10.1016/j.foodchem.2020.127891subject
Has Abstractpub_date
2021-03-01 00:00:00pages
127891eissn
0308-8146issn
1873-7072pii
S0308-8146(20)31753-2journal_volume
339pub_type
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