Abstract：
:A simple, sensitive, accurate and affordable rapid detection of meat species authentication is urgently needed in food industry. In this study, a cross priming amplification (CPA) combining nucleic acid test strip (CPA-Strip) assay for rapid detection of mutton from meat mixture were developed and its feasibility was investigated. In an isothermal CPA system, cytochrome b (cytb) gene as target was amplified at 63°C for 60min. The nucleic acid strip was able to show the corresponding test line in the presence of target gens in 5min. Non-targeting gene interference was not evident. The CPA-Strip has been applied for the detection of 0.1-100% mutton in a thermal treated meat mixtures with a detection limit of a detect limit of 1%. CPA-Strip assay would be a promising simple, rapid and sensitive method for identification of target species in raw and processed meat mixtures.
journal_name
Food Chemjournal_title
Food chemistryauthors
Feng T,Li S,Wang S,Pan Jdoi
10.1016/j.foodchem.2017.08.107subject
Has Abstractpub_date
2018-04-15 00:00:00pages
641-645eissn
0308-8146issn
1873-7072pii
S0308-8146(17)31443-7journal_volume
245pub_type
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