Abstract：
:A novel, easy, and cheap technique for preliminary quantitative evaluation of antiradical activity, based on HPTLC, has been proposed. This method combines chromatographic separation of polar compounds, present in plant extracts, with data analysis by means of image processing software. Bleaching of the purple DPPH colour, caused by substances with antiradical activity, was observed and recorded using a photo camera. ImageJ, a free and open source image processing program was used for quantitative measurements. For evaluation of assay efficiency, the antiradical activity of rose flower extracts (from Rosa rugosa Thunb.) was expressed as Standard Activity Coefficients (SACs), which are relative measures of the activity to the four well known antioxidants; i.e., quercetin, gallic acid, protocatechuic acid, and Trolox. The method uses small amounts of free radical and is easily applicable - only a digital camera with freely available open source software is required.
journal_name
Food Chemjournal_title
Food chemistryauthors
Olech M,Komsta Ł,Nowak R,Cieśla Ł,Waksmundzka-Hajnos Mdoi
10.1016/j.foodchem.2011.10.067subject
Has Abstractpub_date
2012-05-01 00:00:00pages
549-53issue
1eissn
0308-8146issn
1873-7072pii
S0308-8146(11)01520-2journal_volume
132pub_type
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