Abstract：
:Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.
journal_name
Food Chemjournal_title
Food chemistryauthors
Huang Q,Bu T,Zhang W,Yan L,Zhang M,Yang Q,Huang L,Yang B,Hu N,Suo Y,Wang J,Zhang Ddoi
10.1016/j.foodchem.2018.04.085subject
Has Abstractpub_date
2018-10-01 00:00:00pages
48-55eissn
0308-8146issn
1873-7072pii
S0308-8146(18)30725-8journal_volume
262pub_type
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