Abstract：
:Herein, a label-free and dual-readout immunochromatographic test strip (ITS) for the sensitive detection of Escherichia coli (E. coli) O157:H7 by taking advantages of the strong capture ability of Fe3O4@CuS nanostructures (NSs) towards bacteria and their ultrahigh photothermal effects (PTEs) was reported. Especially, without the customarily antibody (Ab)-labeled probe, Fe3O4@CuS NSs could be adsorbed onto the surfaces of bacteria to form Fe3O4@CuS-bacteria conjugates and then trapped by immobilized Abs on the test line (T-line), forming a characteristic yellow band. After direct immunoreactions, the PTEs of Fe3O4@CuS NSs endowed T-line to be irradiated by an 808 nm infrared light, obtaining satisfactory sensitivity and accuracy. Under optimal conditions, E. coli O157:H7, as low as 103 and 102 CFU/mL, could be monitored in colorimetric and photothermal modes. Additionally, E. coli O157:H7 was successfully detected in beef, chicken, milk and honey samples by this proposed platform with a recovery of 80-120%.
journal_name
Food Chemjournal_title
Food chemistryauthors
Zhang M,Bu T,Tian Y,Sun X,Wang Q,Liu Y,Bai F,Zhao S,Wang Ldoi
10.1016/j.foodchem.2020.127398subject
Has Abstractpub_date
2020-12-01 00:00:00pages
127398eissn
0308-8146issn
1873-7072pii
S0308-8146(20)31260-7journal_volume
332pub_type
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