Abstract：
:In this study, a paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) was developed as a screening system for rapid detection of clenbuterol, which is illegally used as a growth promoter for food-producing animals. The microfluidic paper-based analytical device (μPAD) was combined with ELISA and the intrinsic properties of paper allowed the entrapment of antibody through cellulosic fibres, validating to be an alternative to 96-well ELISA microplate for food safety monitoring. Detection of clenbuterol in milk was achieved by measuring the intensity of colour change that was proportional to the analyte concentration with a detection limit of 0.2 ppb. The μPAD effectively reduces the cost, volume of reagents, and time required to run ELISA for food sample testing.
journal_name
Food Chemjournal_title
Food chemistryauthors
Ma L,Nilghaz A,Choi JR,Liu X,Lu Xdoi
10.1016/j.foodchem.2017.12.022subject
Has Abstractpub_date
2018-04-25 00:00:00pages
437-441eissn
0308-8146issn
1873-7072pii
S0308-8146(17)31961-1journal_volume
246pub_type
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