Abstract：
:Food adulteration and fraud is driven by economic interests; it is thus necessary to establish a high-through method that allows quantitative identification of familiar animal and plant proteins for global use. In this study, a sensitive mass spectrometric approach for the detection of eight species, including pork, beef, lamb, chicken, duck, soy, peanut, and pea, is presented and the heat stability and specificity of screened peptides are verified. To improve screening efficiency of specific peptides, several key data searching parameters, including peptides, sequence lengths, sequence coverage, and unique peptides, are investigated. Using this approach, it is possible to detect a 0.5% contamination of any of the eight species. The method is proven to have high sensitivity, specificity, repeatability, and a low quantitative detection limit with respect to adulteration of diverse types of meat products.
journal_name
Food Chemjournal_title
Food chemistryauthors
Li Y,Zhang Y,Li H,Zhao W,Guo W,Wang Sdoi
10.1016/j.foodchem.2017.09.066subject
Has Abstractpub_date
2018-04-15 00:00:00pages
125-131eissn
0308-8146issn
1873-7072pii
S0308-8146(17)31522-4journal_volume
245pub_type
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