Abstract：
:This study was carried out to identify the glycerophosphocholine (GPCho), glycerophosphoethanolamine (GPEtn) and glycerophosphoserine (GPSer) compositions in lipids extracted from mussels using Folch, Bligh-Dyer and methyl-tert-butyl ether (MTBE) methods by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). The molecular species of GPCho, GPEtn and GPSer were characterized according to the MS and MS/MS information. A semi-quantitative method using internal standard was established to compare the difference in glycerophospholipids (GP) between samples recovered with different methods. At least 212, 230 and 206 GP species were identified, respectively, from lipids recovered by Folch, Bligh-Dyer and MTBE methods. Most of the abundant GP species in mussels contained EPA and DHA. Some GP species with low content were not present in lipids recovered by the Folch and MTBE methods when compared with that recovered by the Bligh-Dyer method. However, for most GP species in lipids recovered by different methods, no quantitative differences existed.
journal_name
Food Chemjournal_title
Food chemistryauthors
Yin FW,Zhou DY,Zhao Q,Liu ZY,Hu XP,Liu YF,Song L,Zhou X,Qin L,Zhu BW,Shahidi Fdoi
10.1016/j.foodchem.2016.06.094subject
Has Abstractpub_date
2016-12-15 00:00:00pages
344-351eissn
0308-8146issn
1873-7072pii
S0308-8146(16)30996-7journal_volume
213pub_type
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