A study on biomimetic immunoassay-capillary electrophoresis method based on molecularly imprinted polymer for determination of trace trichlorfon residue in vegetables.

Abstract:

:Pesticide residue in vegetables is a serious problem that has adverse effects on human health. In our study, we designed and synthesized a molecularly imprinted polymer that can selectively recognize trichlorfon. Using the polymer material as biomimetic antibody, we developed a biomimetic immunoassay-capillary electrophoresis method with improved sensitivity for the detection of trichlorfon. We evaluated the competitive reactions between HRP labeled trichlorfon hapten and free trichlorfon with the biomimetic antibody. Factors that affected the sensitivity of our method were tested in detail. Under optimal conditions, the limit of detection (LOD, IC15) and the sensitivity (IC50) of this method were 0.16mgL-1 and 0.13μgL-1 for trichlorfon. We used this method to determine the trichlorfon spiked in the kidney bean and cucumber samples with recoveries ranging from 78.8% to 103%. We also detected residual trichlorfons in the leek samples, and these results were verified by gas chromatography method.

journal_name

Food Chem

journal_title

Food chemistry

authors

Li J,Lu J,Qiao X,Xu Z

doi

10.1016/j.foodchem.2016.11.028

subject

Has Abstract

pub_date

2017-04-15 00:00:00

pages

1285-1290

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(16)31851-9

journal_volume

221

pub_type

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