Abstract:
:Pesticide residue in vegetables is a serious problem that has adverse effects on human health. In our study, we designed and synthesized a molecularly imprinted polymer that can selectively recognize trichlorfon. Using the polymer material as biomimetic antibody, we developed a biomimetic immunoassay-capillary electrophoresis method with improved sensitivity for the detection of trichlorfon. We evaluated the competitive reactions between HRP labeled trichlorfon hapten and free trichlorfon with the biomimetic antibody. Factors that affected the sensitivity of our method were tested in detail. Under optimal conditions, the limit of detection (LOD, IC15) and the sensitivity (IC50) of this method were 0.16mgL-1 and 0.13μgL-1 for trichlorfon. We used this method to determine the trichlorfon spiked in the kidney bean and cucumber samples with recoveries ranging from 78.8% to 103%. We also detected residual trichlorfons in the leek samples, and these results were verified by gas chromatography method.
journal_name
Food Chemjournal_title
Food chemistryauthors
Li J,Lu J,Qiao X,Xu Zdoi
10.1016/j.foodchem.2016.11.028subject
Has Abstractpub_date
2017-04-15 00:00:00pages
1285-1290eissn
0308-8146issn
1873-7072pii
S0308-8146(16)31851-9journal_volume
221pub_type
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