Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.

Abstract:

:In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Carossino M,Ip HS,Richt JA,Shultz K,Harper K,Loynachan AT,Del Piero F,Balasuriya UBR

doi

10.1007/s00705-020-04737-w

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

2373-2377

issue

10

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-020-04737-w

journal_volume

165

pub_type

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