Abstract:
:In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Carossino M,Ip HS,Richt JA,Shultz K,Harper K,Loynachan AT,Del Piero F,Balasuriya UBRdoi
10.1007/s00705-020-04737-wsubject
Has Abstractpub_date
2020-10-01 00:00:00pages
2373-2377issue
10eissn
0304-8608issn
1432-8798pii
10.1007/s00705-020-04737-wjournal_volume
165pub_type
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