Abstract:
:Chlorella is a unicellular green microalga that has been used in fields such as bioenergy production and food supplementation. In this study, two promoters of N (nitrogen) deficiency-inducible Chlorella vulgaris N Deficiency Inducible (CvNDI) genes were isolated from Chlorella vulgaris UTEX 395. These promoters were used for the production of a recombinant protein, human granulocyte-colony stimulating factor (hG-CSF) in Chlorella vulgaris UTEX 395 and Chlorella sp. ArM0029B. To efficiently secrete the hG-CSF, the protein expression vectors incorporated novel signal peptides obtained from a secretomics analysis of Chlorella spp. After a stable transformation of those vectors with a codon-optimized hG-CSF sequence, hG-CSF polypeptides were successfully produced in the spent media of the transgenic Chlorella. To our knowledge, this is the first report of recombinant protein expression using endogenous gene components of Chlorella.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Shin JH,Choi J,Jeon J,Kumar M,Lee J,Jeong WJ,Kim SRdoi
10.1038/s41598-020-69620-9subject
Has Abstractpub_date
2020-07-29 00:00:00pages
12713issue
1issn
2045-2322pii
10.1038/s41598-020-69620-9journal_volume
10pub_type
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