The establishment of new protein expression system using N starvation inducible promoters in Chlorella.

Abstract:

:Chlorella is a unicellular green microalga that has been used in fields such as bioenergy production and food supplementation. In this study, two promoters of N (nitrogen) deficiency-inducible Chlorella vulgaris N Deficiency Inducible (CvNDI) genes were isolated from Chlorella vulgaris UTEX 395. These promoters were used for the production of a recombinant protein, human granulocyte-colony stimulating factor (hG-CSF) in Chlorella vulgaris UTEX 395 and Chlorella sp. ArM0029B. To efficiently secrete the hG-CSF, the protein expression vectors incorporated novel signal peptides obtained from a secretomics analysis of Chlorella spp. After a stable transformation of those vectors with a codon-optimized hG-CSF sequence, hG-CSF polypeptides were successfully produced in the spent media of the transgenic Chlorella. To our knowledge, this is the first report of recombinant protein expression using endogenous gene components of Chlorella.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Shin JH,Choi J,Jeon J,Kumar M,Lee J,Jeong WJ,Kim SR

doi

10.1038/s41598-020-69620-9

subject

Has Abstract

pub_date

2020-07-29 00:00:00

pages

12713

issue

1

issn

2045-2322

pii

10.1038/s41598-020-69620-9

journal_volume

10

pub_type

杂志文章
  • Robust Direct Bandgap Characteristics of One- and Two-Dimensional ReS2.

    abstract::Two-dimensional (2D) transition-metal dichalcogenides (TMDs), most notably, MoS2 and WS2, have attracted significant attention due to their sizable and direct bandgap characteristics. Although several interesting MoS2 and WS2-based optoelectronic devices have been reported, their processability and reproducibility are...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13783

    authors: Yu ZG,Cai Y,Zhang YW

    更新日期:2015-09-08 00:00:00

  • Continuous-wave phase-matched molecular optical modulator.

    abstract::In optical modulation, the highest available modulation rate is basically limited to the GHz frequency range at best. This is because optical modulation is often performed using electro-optic or acousto-optic effects that require application of an external signal to solid-state nonlinear optical materials. Here we des...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep20908

    authors: Zaitsu S,Izaki H,Tsuchiya T,Imasaka T

    更新日期:2016-02-18 00:00:00

  • Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid.

    abstract::Advances in genomics have the potential to revolutionize clinical diagnostics. Here, we examine the microbiome of vitreous (intraocular body fluid) from patients who developed endophthalmitis following cataract surgery or intravitreal injection. Endophthalmitis is an inflammation of the intraocular cavity and can lead...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-22416-4

    authors: Kirstahler P,Bjerrum SS,Friis-Møller A,la Cour M,Aarestrup FM,Westh H,Pamp SJ

    更新日期:2018-03-07 00:00:00

  • Combined deficiency of Notch1 and Notch3 causes pericyte dysfunction, models CADASIL, and results in arteriovenous malformations.

    abstract::Pericytes regulate vessel stability and pericyte dysfunction contributes to retinopathies, stroke, and cancer. Here we define Notch as a key regulator of pericyte function during angiogenesis. In Notch1(+/-); Notch3(-/-) mice, combined deficiency of Notch1 and Notch3 altered pericyte interaction with the endothelium a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16449

    authors: Kofler NM,Cuervo H,Uh MK,Murtomäki A,Kitajewski J

    更新日期:2015-11-13 00:00:00

  • Cell population heterogeneity driven by stochastic partition and growth optimality.

    abstract::A fundamental question in biology is how cell populations evolve into different subtypes based on homogeneous processes at the single cell level. Here we show that population bimodality can emerge even when biological processes are homogenous at the cell level and the environment is kept constant. Our model is based o...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-45882-w

    authors: Fernandez-de-Cossio-Diaz J,Mulet R,Vazquez A

    更新日期:2019-06-28 00:00:00

  • Endometrial Mesenchymal Stem/Stromal Cells Modulate the Macrophage Response to Implanted Polyamide/Gelatin Composite Mesh in Immunocompromised and Immunocompetent Mice.

    abstract::The immunomodulatory properties of human endometrial mesenchymal stem cells (eMSC) have not been well characterised. Initial studies showed that eMSC modulated the chronic inflammatory response to a non-degradable polyamide/gelatin mesh in a xenogeneic rat skin wound repair model, but the mechanism remains unclear. In...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24919-6

    authors: Darzi S,Deane JA,Nold CA,Edwards SE,Gough DJ,Mukherjee S,Gurung S,Tan KS,Vashi AV,Werkmeister JA,Gargett CE

    更新日期:2018-04-26 00:00:00

  • Publisher Correction: Membrane association of the bacterial riboregulator Hfq and functional perspectives.

    abstract::A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-017-15019-y

    authors: Malabirade A,Morgado-Brajones J,Trépout S,Wien F,Marquez I,Seguin J,Marco S,Velez M,Arluison V

    更新日期:2017-11-10 00:00:00

  • Binding modes of environmental endocrine disruptors to human serum albumin: insights from STD-NMR, ITC, spectroscopic and molecular docking studies.

    abstract::Given that bisphenols have an endocrine-disrupting effect on human bodies, thoroughly exposing their potential effects at the molecular level is important. Saturation transfer difference (STD) NMR-based binding studies were performed to investigate the binding potential of two bisphenol representatives, namely, bisphe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11604-3

    authors: Yang H,Huang Y,Liu J,Tang P,Sun Q,Xiong X,Tang B,He J,Li H

    更新日期:2017-09-11 00:00:00

  • Velvet-mediated repression of β-glucan synthesis in Aspergillus nidulans spores.

    abstract::Beta-glucans are a heterologous group of fibrous glucose polymers that are a major constituent of cell walls in Ascomycetes and Basidiomycetes fungi. Synthesis of β (1,3)- and (1,6)-glucans is coordinated with fungal cell growth and development, thus, is under tight genetic regulation. Here, we report that β-glucan sy...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep10199

    authors: Park HS,Man Yu Y,Lee MK,Jae Maeng P,Chang Kim S,Yu JH

    更新日期:2015-05-11 00:00:00

  • Gravitational vortices and clump formation in Saturn's F ring during an encounter with Prometheus.

    abstract::Saturn rings are most beautiful and dynamic places in the solar system, consisting of ice particles in a constant battle between the gravitational forces of Saturn and its many moons. Fan, spiral, propellers, moonlets and streamer-channels observed by CASSINI in the F-ring have been attributed to encounters by Prometh...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01276

    authors: Sutton PJ,Kusmartsev FV

    更新日期:2013-01-01 00:00:00

  • Flood-conditioned place aversion as a novel non-pharmacological aversive learning procedure in mice.

    abstract::The place conditioning paradigm is an efficient, widely-used method to study mechanisms that underlie appetitive or aversive learning and memory processes. However, pharmacological agents used to induce conditioned place preference (CPP) or aversion (CPA) can per se interfere with learning and memory processing, hence...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-25568-5

    authors: Goltseker K,Barak S

    更新日期:2018-05-08 00:00:00

  • A Physarum Centrality Measure of the Human Brain Network.

    abstract::The most important goals of brain network analyses are to (a) detect pivotal regions and connections that contribute to disproportionate communication flow, (b) integrate global information, and (c) increase the brain network efficiency. Most centrality measures assume that information propagates in networks with the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-42322-7

    authors: Kwon H,Choi YH,Lee JM

    更新日期:2019-04-11 00:00:00

  • Pharmacological profile and efficiency in vivo of diflapolin, the first dual inhibitor of 5-lipoxygenase-activating protein and soluble epoxide hydrolase.

    abstract::Arachidonic acid (AA) is metabolized to diverse bioactive lipid mediators. Whereas the 5-lipoxygenase-activating protein (FLAP) facilitates AA conversion by 5-lipoxygenase (5-LOX) to pro-inflammatory leukotrienes (LTs), the soluble epoxide hydrolase (sEH) degrades anti-inflammatory epoxyeicosatrienoic acids (EETs). Ac...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09795-w

    authors: Garscha U,Romp E,Pace S,Rossi A,Temml V,Schuster D,König S,Gerstmeier J,Liening S,Werner M,Atze H,Wittmann S,Weinigel C,Rummler S,Scriba GK,Sautebin L,Werz O

    更新日期:2017-08-24 00:00:00

  • Identification of host genes leading to West Nile virus encephalitis in mice brain using RNA-seq analysis.

    abstract::Differential host responses may be critical determinants of distinct pathologies of West Nile virus (WNV) NY99 (pathogenic) and WNV Eg101 (non-pathogenic) strains. We employed RNA-seq technology to analyze global differential gene expression in WNV-infected mice brain and to identify the host cellular factors leading ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep26350

    authors: Kumar M,Belcaid M,Nerurkar VR

    更新日期:2016-05-23 00:00:00

  • Influence of the human papillomavirus on the radio-responsiveness of cancer stem cells in head and neck cancers.

    abstract::A growing proportion of head and neck cancers (HNC) result from HPV infection. Between HNC aetiological groups (HPV positive and HPV negative) clinical evidence demonstrates significantly better treatment response among HPV positive cancers. Cancer stem cells (CSCs) are identified in HNC tumour populations as agents o...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59654-4

    authors: Reid P,Staudacher AH,Marcu LG,Olver I,Moghaddasi L,Brown MP,Bezak E

    更新日期:2020-02-17 00:00:00

  • Involvement of Endoplasmic Reticulum Stress, Autophagy, and Apoptosis in Advanced Glycation End Products-Induced Glomerular Mesangial Cell Injury.

    abstract::Advanced glycation end-products (AGEs)-induced mesangial cell death is one of major causes of glomerulus dysfunction in diabetic nephropathy. Both endoplasmic reticulum (ER) stress and autophagy are adaptive responses in cells under environmental stress and participate in the renal diseases. The role of ER stress and ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep34167

    authors: Chiang CK,Wang CC,Lu TF,Huang KH,Sheu ML,Liu SH,Hung KY

    更新日期:2016-09-26 00:00:00

  • Discovery of annual growth in a modern olive branch based on carbon isotopes and implications for the Bronze Age volcanic eruption of Santorini.

    abstract::The volcanic eruption of Santorini in the Bronze Age left detectable debris across the Mediterranean, serving as an anchor in time for the region, synchronizing chronologies of different sites. However, dating the eruption has been elusive for decades, as radiocarbon indicates a date about a century earlier than archa...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79024-4

    authors: Ehrlich Y,Regev L,Boaretto E

    更新日期:2021-01-12 00:00:00

  • Clinicopathologic significance of MYD88 L265P mutation in diffuse large B-cell lymphoma: a meta-analysis.

    abstract::The precise clinicopathologic significance of myeloid differentiation primary response gene (MYD88) L265P mutation in diffuse large B-cell lymphomas (DLBCLs) remains elusive. To investigate the frequency and clinicopathologic significance of the MYD88 L265P mutation in DLBCLs, we conducted a meta-analysis of 40 publis...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/s41598-017-01998-5

    authors: Lee JH,Jeong H,Choi JW,Oh H,Kim YS

    更新日期:2017-05-11 00:00:00

  • Characterization of the effects of immunomodulatory drug fingolimod (FTY720) on human T cell receptor signaling pathways.

    abstract::Immune responses against gene therapy products limit its therapeutic efficacy and present a safety risk. Identification of agents that blunt immune reactions may aid in developing novel immunomodulatory therapies. Fingolimod (FTY720) is an FDA approved immunomodulatory drug for treating multiple sclerosis that inhibit...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-29355-0

    authors: Baer A,Colon-Moran W,Bhattarai N

    更新日期:2018-07-19 00:00:00

  • Growth and Morbidity of Gambian Infants are Influenced by Maternal Milk Oligosaccharides and Infant Gut Microbiota.

    abstract::Human milk oligosaccharides (HMOs) play an important role in the health of an infant as substrate for beneficial gut bacteria. Little is known about the effects of HMO composition and its changes on the morbidity and growth outcomes of infants living in areas with high infection rates. Mother's HMO composition and inf...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40466

    authors: Davis JC,Lewis ZT,Krishnan S,Bernstein RM,Moore SE,Prentice AM,Mills DA,Lebrilla CB,Zivkovic AM

    更新日期:2017-01-12 00:00:00

  • A sensitive scoring system for the longitudinal clinical evaluation and prediction of lethal disease outcomes in newborn mice.

    abstract::Neonatal animal models are increasingly employed in order to unravel age-specific disease mechanisms. Appropriate tools objectifying the clinical condition of murine neonates are lacking. In this study, we tested a scoring system specifically designed for newborn mice that relies on clinical observation and examinatio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-42414-4

    authors: Fehlhaber B,Heinemann AS,Rübensam K,Willers M,Völlger L,Pfeifer S,von Köckritz-Blickwede M,Viemann D

    更新日期:2019-04-11 00:00:00

  • Trivalent Y3+ ionic sensor development based on (E)-Methyl-N'-nitrobenzylidene-benzenesulfonohydrazide (MNBBSH) derivatives modified with nafion matrix.

    abstract::(E)-Methyl-N'-nitrobenzylidene-benzenesulfonohydrazide (MNBBSH) compounds were synthesized using a condensation procedure from the derivatives of nitrobenzaldehyde and 4-Methyl-benzenesulfonylhydrazine, which crystallized in ethanol and methanol as well as characterized by FTIR, UV-Vis, 1H-NMR, and 13C-NMR. MNBBSH str...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05703-4

    authors: Hussain MM,Rahman MM,Arshad MN,Asiri AM

    更新日期:2017-07-19 00:00:00

  • Synergistic adsorption-photocatalytic degradation effect and norfloxacin mechanism of ZnO/ZnS@BC under UV-light irradiation.

    abstract::Norfloxacin (NOF) is an environmentally harmful and ubiquitous aquatic pollutant with extensive production and application. In this study, a novel composition named carbon-based composite photocatalytic material of zinc oxide and zinc sulphide (ZnO/ZnS@BC) was successfully obtained by the impregnation-roasting method ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-68517-x

    authors: Liu W,He T,Wang Y,Ning G,Xu Z,Chen X,Hu X,Wu Y,Zhao Y

    更新日期:2020-07-17 00:00:00

  • Variations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state.

    abstract::Ball-milling utilizes mechanical stress to modify properties of carbon nanotubes (CNTs) including size, capping, and functionalization. Ball-milling, however, may introduce structural defects resulting in altered CNT-biomolecule interactions. Nanomaterial-biomolecule interactions result in the formation of the biocoro...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-08896-w

    authors: Raghavendra AJ,Fritz K,Fu S,Brown JM,Podila R,Shannahan JH

    更新日期:2017-08-16 00:00:00

  • Distillation of photon entanglement using a plasmonic metamaterial.

    abstract::Plasmonics is a rapidly emerging platform for quantum state engineering with the potential for building ultra-compact and hybrid optoelectronic devices. Recent experiments have shown that despite the presence of decoherence and loss, photon statistics and entanglement can be preserved in single plasmonic systems. This...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep18313

    authors: Asano M,Bechu M,Tame M,Kaya Özdemir Ş,Ikuta R,Güney DÖ,Yamamoto T,Yang L,Wegener M,Imoto N

    更新日期:2015-12-16 00:00:00

  • Myth busting? Effects of embryo positioning and egg turning on hatching success in the water snake Natrix maura.

    abstract::It is a common belief that reptile eggs should not be turned after oviposition once the embryo has attached itself to the inner membrane of the shell as it might kill developing embryos. Here, we used 338 eggs from 32 clutches of the water snake Natrix maura to (1) thoroughly describe natural clutch arrangement, (2) e...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13385

    authors: Aubret F,Blanvillain G,Kok PJ

    更新日期:2015-08-21 00:00:00

  • Synergistic suppression of autoimmune arthritis through concurrent treatment with tolerogenic DC and MSC.

    abstract::Rheumatoid arthritis (RA) is an autoimmune disease characterized by progressive immune-mediated joint deterioration. Current treatments are not antigen specific and are associated with various adverse. We have previously demonstrated that tolerogenic dendritic cells (Tol-DC) are potent antigen-specific immune regulato...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep43188

    authors: Li R,Zhang Y,Zheng X,Peng S,Yuan K,Zhang X,Min W

    更新日期:2017-02-23 00:00:00

  • Ag+ doped into azo-linked conjugated microporous polymer for volatile iodine capture and detection of heavy metal ions.

    abstract::We herein report the construction of a novel azo-linked conjugated microporous polymers (Ag@AzoTPE-CMP), which possesses permanent porous structure and Ag+ loading up of 7.62% in the skeleton as effective sorption sites. Ag@AzoTPE-CMP shows considerable adsorption capacity of iodine of 202 wt% in iodine vapor at 350 K...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-32383-5

    authors: Liu M,Yao C,Liu C,Xu Y

    更新日期:2018-09-19 00:00:00

  • Analysis of IS6110 insertion sites provide a glimpse into genome evolution of Mycobacterium tuberculosis.

    abstract::Insertion sequence (IS) 6110 is found at multiple sites in the Mycobacterium tuberculosis genome and displays a high degree of polymorphism with respect to copy number and insertion sites. Therefore, IS6110 is considered to be a useful molecular marker for diagnosis and strain typing of M. tuberculosis. Generally IS61...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep12567

    authors: Roychowdhury T,Mandal S,Bhattacharya A

    更新日期:2015-07-28 00:00:00

  • Hydrogen-accelerated spontaneous microcracking in high-strength aluminium alloys.

    abstract::Aluminium alloys are re-evaluated as most feasible way to satisfy the industrial needs of light-weight structural materials. However, unlike conventional structural metals such as iron and titanium, aluminium does not have easily accessible secondary phases, which means that aluminium-based alloys cannot be strengthen...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-58834-6

    authors: Tsuru T,Shimizu K,Yamaguchi M,Itakura M,Ebihara K,Bendo A,Matsuda K,Toda H

    更新日期:2020-04-06 00:00:00