Selective autophagy inhibition through disruption of the PIK3C3-containing complex I.

abstract：:Autophagy is a major pathway for the degradation of long-lived proteins and cytoplasmic organelles and an essential part of programmed cell death, as well. Our findings indicate that programmed cell death of the ovarian follicle cells in the higher Diptera species Bactrocera oleae and Ceratitis capitata manifests feat...

journal_title：Autophagy

authors： Nezis IP,Stravopodis DJ,Margaritis LH,Papassideri IS

更新日期：2006-10-01 00:00:00

abstract：:Autophagosomes arise in yeast and animals from the sealing of a cup-shaped double-membrane precursor, the phagophore. The concerted action of about 30 evolutionarily conserved autophagy related (ATG) proteins lies at the core of this process. However, the mechanisms allowing phagophore generation and its differentiati...

journal_title：Autophagy

authors： Le Bars R,Marion J,Satiat-Jeunemaitre B,Bianchi MW

更新日期：2014-10-01 00:00:00

abstract：:(Macro)autophagy is a membrane-trafficking process that serves to sequester cellular constituents in organelles termed autophagosomes, which target their degradation in the lysosome. Autophagy operates at basal levels in all cells where it serves as a homeostatic mechanism to maintain cellular integrity. The levels an...

journal_title：Autophagy

authors： Wilkinson S,Croft DR,O'Prey J,Meedendorp A,O'Prey M,Dufès C,Ryan KM

更新日期：2011-11-01 00:00:00

abstract：:A shared neuropathological hallmark in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) is nuclear clearance and cytoplasmic aggregation of TARDBP/TDP-43 (TAR DNA binding protein). We previously showed that the ability of TARDBP to repress nonconserved cryptic exons was impaired in brains of patie...

journal_title：Autophagy

authors： Donde A,Sun M,Jeong YH,Wen X,Ling J,Lin S,Braunstein K,Nie S,Wang S,Chen L,Wong PC

更新日期：2020-04-01 00:00:00

abstract：:The incidence of pancreatic adenocarcinoma is increasing with more than 43,000 predicted new cases in the US and 65,000 in Europe this year. Pancreatic cancer patients have a short life expectancy with less than 3-4% 5-y survival, which results in an equivalent incidence and mortality rate. One of the major challenges...

journal_title：Autophagy

authors： Hamidi T,Cano CE,Grasso D,Garcia MN,Sandi MJ,Calvo EL,Dagorn JC,Lomberk G,Goruppi S,Urrutia R,Carracedo A,Velasco G,Iovanna JL

更新日期：2013-01-01 00:00:00

abstract：:Throughout their life, cells must maintain homeostasis while facing constantly fluctuating demands on their different organelles. A major mechanism for the homeostatic control of organelle function is the unfolded protein response (UPR), a signaling pathway that triggers a comprehensive remodeling of the endoplasmic r...

journal_title：Autophagy

authors： Bernales S,Schuck S,Walter P

更新日期：2007-05-01 00:00:00

abstract：:Autophagy is a complex, multi-step and biologically important pathway mediated by autophagosomes and autolysosomes. Accurately dissecting and detecting different stages of autophagy is important to elucidate its molecular mechanism and thereby facilitate the discovery of pharmaceutical molecules. We herein reported a ...

journal_title：Autophagy

authors： Chen JJ,Jing J,Chang H,Rong Y,Hai Y,Tang J,Zhang JL,Xu P

更新日期：2013-06-01 00:00:00

abstract：:Cytotoxic necrotizing factor 1 is a bacterial protein toxin from Escherichia coli that is able to activate the Rho GTPases and to hinder apoptosis and mitotic catastrophe. Upon exposure to toxin, cells undergo a complex framework of changes, including cytoskeleton remodeling and multinucleation. These cells also show ...

journal_title：Autophagy

authors： Fiorentini C,Malorni W

更新日期：2006-10-01 00:00:00

abstract：:Several recent reports have demonstrated that autophagy is induced in response to hypoxia in cultured cells. However, the mechanism and consequence of hypoxia-induced autophagy remains unclear as there is no consensus between these studies. In our recent report we show that, in human cancer cells, hypoxia cooperates w...

journal_title：Autophagy

authors： Wilkinson S,Ryan KM

更新日期：2009-10-01 00:00:00

abstract：:Protein synthesis and autophagy work as two opposing processes to control cell growth in response to nutrient supply. The mammalian/mechanistic target of rapamycin complex 1 (mTORC1) pathway, which acts as a master regulator to control protein synthesis, has recently been shown to inhibit autophagy by phosphorylating ...

journal_title：Autophagy

authors： Dunlop EA,Hunt DK,Acosta-Jaquez HA,Fingar DC,Tee AR

更新日期：2011-07-01 00:00:00

abstract：:Macroautophagy is a major lysosomal degradation pathway for cellular components in eukaryotic cells. Baseline macroautophagy is important for quality control of the cytoplasm in order to avoid the accumulation of cytotoxic products. Its stimulation by various stressful situations, including nutrient starvation, is imp...

journal_title：Autophagy

authors： Tuloup-Minguez V,Greffard A,Codogno P,Botti J

更新日期：2011-01-01 00:00:00

abstract：:Mitochondrial dysfunction is associated with the occurrence of a variety of neurodegenerative diseases, especially Alzheimer disease (AD). As a mitochondrial quality control process, mitophagy is greatly inhibited in AD; increasing evidence shows that the induction of mitophagy is an effective therapeutic intervention...

journal_title：Autophagy

authors： Cen X,Xu X,Xia H

更新日期：2020-12-20 00:00:00

abstract：:Phosphoinositides (PIs) are a group of low-abundance phospholipids that play a critical role in the control of organelle and membrane traffic. There is strong evidence that specific PIs are also important for the regulation of autophagy. PIs are modified by a complex network of lipid kinases and phosphatases. A recent...

journal_title：Autophagy

authors： Gibbs EM,Feldman EL,Dowling JJ

更新日期：2010-08-01 00:00:00

abstract：:Retinal ischemia is a major cause of vision loss and a common underlying mechanism associated with diseases, such as diabetic retinopathy and central retinal artery occlusion. We have previously demonstrated the robust neuroprotection in retina induced by post-conditioning (post-C), a brief period of ischemia, 24 h, f...

journal_title：Autophagy

authors： Mathew B,Chennakesavalu M,Sharma M,Torres LA,Stelman CR,Tran S,Patel R,Burg N,Salkovski M,Kadzielawa K,Seiler F,Aldrich LN,Roth S

更新日期：2020-05-26 00:00:00

abstract：:Mutations in the ER-associated VAPB/ALS8 protein cause amyotrophic lateral sclerosis and spinal muscular atrophy. Previous studies have argued that ER stress may underlie the demise of neurons. We find that loss of VAP proteins (VAPs) leads to an accumulation of aberrant lysosomes and impairs lysosomal degradation. VA...

journal_title：Autophagy

authors： Mao D,Lin G,Tepe B,Zuo Z,Tan KL,Senturk M,Zhang S,Arenkiel BR,Sardiello M,Bellen HJ

更新日期：2019-07-01 00:00:00

abstract：:Microtubule-associated protein light chain 3 (LC3) is now widely used to monitor autophagy. One approach is to detect LC3 conversion (LC3-I to LC3-II) by immunoblot analysis because the amount of LC3-II is clearly correlated with the number of autophagosomes. However, LC3-II itself is degraded by autophagy, making int...

journal_title：Autophagy

authors： Mizushima N,Yoshimori T

更新日期：2007-11-01 00:00:00

abstract：:LC3 is a widely used marker of autophagosomes in mammalian cells. However, in addition to its autophagosomal localization, GFP-LC3 is often found associated with protein aggregates that are formed in an autophagy-independent manner. In addition, LC3 directly interacts with p62/SQSTM1 (hereafter named p62), a common co...

journal_title：Autophagy

authors： Shvets E,Elazar Z

更新日期：2008-11-01 00:00:00

abstract：:SPHK1 (sphingosine kinase 1), a regulator of sphingolipid metabolites, plays a causal role in the development of hepatocellular carcinoma (HCC) through augmenting HCC invasion and metastasis. However, the mechanism by which SPHK1 signaling promotes invasion and metastasis in HCC remains to be clarified. Here, we repor...

journal_title：Autophagy

authors： Liu H,Ma Y,He HW,Zhao WL,Shao RG

更新日期：2017-05-04 00:00:00

abstract：:Burkholderia pseudomallei is the causative agent of melioidosis, a tropical infection of humans and other animals. The bacterium is an intracellular pathogen that can escape from endosomes into the host cytoplasm, where it replicates and infects adjacent cells. We investigated the role played by autophagy in the intra...

journal_title：Autophagy

authors： Cullinane M,Gong L,Li X,Lazar-Adler N,Tra T,Wolvetang E,Prescott M,Boyce JD,Devenish RJ,Adler B

更新日期：2008-08-01 00:00:00

abstract：:Neurons are highly polarized cells composed of two distinct domains, the axon and the somatodendritic domain. Although AMPA-type glutamate receptors, which mediate fast excitatory neurotransmission in the vertebrate CNS, are preferentially expressed in the somatodendritic domain, the molecular mechanisms underlying su...

journal_title：Autophagy

authors： Matsuda S,Yuzaki M

更新日期：2008-08-01 00:00:00

abstract：:During (macro)autophagy, cells form transient organelles, termed autophagosomes, to target a broad spectrum of substrates for degradation critical to cellular and organismal health. Driven by rapid membrane assembly, an initially small vesicle (phagophore) elongates into a large cup-shaped structure to engulf substrat...

journal_title：Autophagy

authors： Schütter M,Graef M

更新日期：2020-04-01 00:00:00

abstract：:Macroautophagy/autophagy is a catabolic process that is essential for cellular homeostasis. How autophagosomal vesicle forms in a spatio-temporally regulated manner remains elusive. Our recent study revealed that small GTPase, RAB37 (RAB37, member RAS oncogene family), functions as a key organizer of autophagosomal me...

journal_title：Autophagy

authors： Song Y,Shang D,Cheng H,Zhou R

更新日期：2018-01-01 00:00:00

abstract：:Upon completion of cytokinesis, the midbody ring is transported asymmetrically into one of the two daughter cells where it becomes a midbody ring derivative that is degraded by autophagy. In this study we showed that the ubiquitin-binding autophagy receptor SQSTM1/p62 and the interacting adaptor protein WDFY3/ALFY for...

journal_title：Autophagy

authors： Isakson P,Lystad AH,Breen K,Koster G,Stenmark H,Simonsen A

更新日期：2013-12-01 00:00:00

abstract：:Deficiency in decidualization has been widely regarded as an important cause of spontaneous abortion. Generalized decidualization also includes massive infiltration and enrichment of NK cells. However, the underlying mechanism of decidual NK (dNK) cell residence remains largely unknown. Here, we observe that the incre...

journal_title：Autophagy

authors： Lu H,Yang HL,Zhou WJ,Lai ZZ,Qiu XM,Fu Q,Zhao JY,Wang J,Li DJ,Li MQ

更新日期：2020-11-01 00:00:00

abstract：:The endothelium plays a central role in the regulation of vascular wall cellularity and tone by secreting an array of mediators of importance in intercellular communication. Nutrient deprivation of human endothelial cells (EC) evokes unconventional forms of secretion leading to the release of nanovesicles distinct fro...

journal_title：Autophagy

authors： Sirois I,Groleau J,Pallet N,Brassard N,Hamelin K,Londono I,Pshezhetsky AV,Bendayan M,Hébert MJ

更新日期：2012-06-01 00:00:00

abstract：:The accumulation of ubiquitin-positive protein aggregates has been implicated in the pathogenesis of neurodegenerative diseases, heart disease and diabetes. Emerging evidence indicates that the autophagy lysosomal pathway plays a critical role in the clearance of ubiquitin aggregates, a process that is mediated by the...

journal_title：Autophagy

authors： Fan W,Tang Z,Chen D,Moughon D,Ding X,Chen S,Zhu M,Zhong Q

更新日期：2010-07-01 00:00:00

abstract：:Coxsackievirus B3 (CVB3) has previously been shown to utilize autophagy in an advantageous manner during the course of infection of the host cell. However, few studies have determined whether stem cells induce autophagy in a similar fashion, and whether virus-induced autophagy occurs following infection of stem cells....

journal_title：Autophagy

authors： Tabor-Godwin JM,Tsueng G,Sayen MR,Gottlieb RA,Feuer R

更新日期：2012-06-01 00:00:00

abstract：:The ubiquitin-proteasome system (UPS) and the autophagy-lysosomal pathway (ALP) are the two most important components of cellular mechanisms for protein degradation. In the present study we investigated the functional relationship of the two systems and the interactional role of p53 in vitro. Our study showed that the...

journal_title：Autophagy

authors： Du Y,Yang D,Li L,Luo G,Li T,Fan X,Wang Q,Zhang X,Wang Y,Le W

更新日期：2009-07-01 00:00:00

abstract：:To study mitochondrial protein age dynamics, we targeted a time-sensitive fluorescent protein, MitoTimer, to the mitochondrial matrix. Mitochondrial age was revealed by the integrated portions of young (green) and old (red) MitoTimer protein. Mitochondrial protein age was dependent on turnover rates as pulsed synthesi...

journal_title：Autophagy

authors： Ferree AW,Trudeau K,Zik E,Benador IY,Twig G,Gottlieb RA,Shirihai OS

更新日期：2013-11-01 00:00:00

abstract：:The relationship between macroautophagy/autophagy and miRNA in regulating cancer cell motility is not clearly delineated. Here, we found that induction of BECN1-dependent or -independent autophagy decreased ubiquitin-binding proteins SQSTM1/p62 and CALCOCO2/NDP52. Downregulation of SQSTM1 (but not CALCOCO2) led to a d...

journal_title：Autophagy

authors： Liao CC,Ho MY,Liang SM,Liang CM

更新日期：2018-01-01 00:00:00