Abstract:
:To study mitochondrial protein age dynamics, we targeted a time-sensitive fluorescent protein, MitoTimer, to the mitochondrial matrix. Mitochondrial age was revealed by the integrated portions of young (green) and old (red) MitoTimer protein. Mitochondrial protein age was dependent on turnover rates as pulsed synthesis, decreased import, or autophagic inhibition all increased the proportion of aged MitoTimer protein. Mitochondrial fusion promotes the distribution of young mitochondrial protein across the mitochondrial network as cells lacking essential fusion genes Mfn1 and Mfn2 displayed increased heterogeneity in mitochondrial protein age. Experiments in hippocampal neurons illustrate that the distribution of older and younger mitochondrial protein within the cell is determined by subcellular spatial organization and compartmentalization of mitochondria into neurites and soma. This effect was altered by overexpression of mitochondrial transport protein, RHOT1/MIRO1. Collectively our data show that distribution of young and old protein in the mitochondrial network is dependent on turnover, fusion, and transport.
journal_name
Autophagyjournal_title
Autophagyauthors
Ferree AW,Trudeau K,Zik E,Benador IY,Twig G,Gottlieb RA,Shirihai OSdoi
10.4161/auto.26503subject
Has Abstractpub_date
2013-11-01 00:00:00pages
1887-96issue
11eissn
1554-8627issn
1554-8635pii
26503journal_volume
9pub_type
杂志文章相关文献
Autophagy文献大全abstract::Flies expressing the most common Parkinson disease (PD)-related mutation, LRRK2-G2019S, in their dopaminergic neurons show loss of visual function and degeneration of the retina, including mitochondrial abnormalities, apoptosis and autophagy. Since the photoreceptors that degenerate are not dopaminergic, this demonstr...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.24397
更新日期:2013-06-01 00:00:00
abstract::Based on my reading, and on my own experience, I have come to realize that people learn in different ways, and this can include the use of different media. This is one reason I have worked with various artists to portray the topic of autophagy through paintings, music and dance. Indeed, comments from members of the au...
journal_title:Autophagy
pub_type: 评论,社论
doi:10.1080/15548627.2019.1630225
更新日期:2019-09-01 00:00:00
abstract::SNCA/α-synuclein aggregation plays a crucial role in synucleinopathies such as Parkinson disease and dementia with Lewy bodies. Aggregating and nonaggregating SNCA species are degraded by the autophagy-lysosomal pathway (ALP). Previously, we have shown that the ALP is not only responsible for SNCA degradation but is a...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.36436
更新日期:2014-01-01 00:00:00
abstract::The link between the deregulation of autophagy and cell death processes can be essential in the development of several neurodegenerative diseases, such as Parkinson disease (PD). However, the molecular mechanism of deregulation of this degradative process in PD patients is unknown. The leucine-rich repeat kinase 2 (LR...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.21270
更新日期:2012-10-01 00:00:00
abstract::Retinal ischemia is a major cause of vision loss and a common underlying mechanism associated with diseases, such as diabetic retinopathy and central retinal artery occlusion. We have previously demonstrated the robust neuroprotection in retina induced by post-conditioning (post-C), a brief period of ischemia, 24 h, f...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2020.1767371
更新日期:2020-05-26 00:00:00
abstract::Autophagy and microRNA (miRNA) are important regulators during cancer cell tumorigenesis. Impaired autophagy and high expression of the oncogenic microRNA MIR224 are prevalent in hepatocellular carcinoma (HCC); however, the relationship between the 2 phenomena remains elusive. In this study, we are the first to reveal...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.29959
更新日期:2014-09-01 00:00:00
abstract::In yeast, Tom22, the central component of the TOMM (translocase of outer mitochondrial membrane) receptor complex, is responsible for the recognition and translocation of synthesized mitochondrial precursor proteins, and its protein kinase CK2-dependent phosphorylation is mandatory for TOMM complex biogenesis and prop...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2017.1403716
更新日期:2018-01-01 00:00:00
abstract::This study summarizes our most recent findings on the mechanisms underlying the cadmium-induced death of mesangial cells, which leads to nephrotoxicity. Multiple pathways participate in cadmium-induced nephrotoxicity. In the ROS-GSK-3beta autophagy pathway, cadmium induces ROS most likely from the mitochondria, and th...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.4.8311
更新日期:2009-05-01 00:00:00
abstract::Macroautophagy/autophagy is emerging as an important process in adult muscle stem cells functions: it regulates metabolic reprogramming during activation from a quiescent state, maintains stemness and prevents senescence. We now show that autophagy is specifically required for neonatal myogenesis and muscle developmen...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2018.1507439
更新日期:2019-01-01 00:00:00
abstract::Macroautophagy/autophagy is critical in maintaining cellular functions and homeostasis. Dynamic regulation of autophagy is associated with development of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH); however, the mechanisms involved in the regulation of autophagy in NAFLD/NASH are n...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2020.1760624
更新日期:2020-07-01 00:00:00
abstract::Autophagy is a fundamental process for anti-viral defense. Not surprisingly, viruses have developed strategies to subvert or use autophagy for their own benefit. In cell culture, autophagy proteins are proviral factors that favor initiation of hepatitis C virus (HCV) infection. Autophagy proteins are required for tran...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.8.10219
更新日期:2009-11-01 00:00:00
abstract::Ferroptosis is a recently discovered form of programmed cell death, but its regulatory mechanisms remain poorly understood. Here, we show that the RNA-binding protein ZFP36/TTP (ZFP36 ring finger protein) plays a crucial role in regulating ferroptosis in hepatic stellate cells (HSCs). Upon exposure to ferroptosis-indu...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2019.1687985
更新日期:2020-08-01 00:00:00
abstract::Photodynamic therapy (PDT) is a procedure that has applications in the selective eradication of neoplasia where sites of malignant lesions are clearly delineated. It is a two-step process whereby cells are first sensitized to light and then photoirradiated. This results in the formation of singlet molecular oxygen and...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.6.1.10220
更新日期:2010-01-01 00:00:00
abstract::Autophagy describes the degradation of unnecessary or dysfunctional cellular components through the lysosomal machinery. Autophagy is essentially required to prevent accumulation of cellular damage and to ensure cellular homeostasis. Indeed, impaired autophagy has been implicated in a variety of different diseases. We...
journal_title:Autophagy
pub_type: 评论,杂志文章
doi:10.4161/auto.25467
更新日期:2013-08-01 00:00:00
abstract::Macroautophagy ("autophagy") is regulated by the same insulin-amino acid-mTOR signaling pathway that controls protein synthesis. Although the literature does not so far include any direct studies confirming this, we expect autophagy to increase during insulin resistance. We discuss the possibility that this may be a u...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.4449
更新日期:2007-09-01 00:00:00
abstract::Dysfunction of macroautophagy/autophagy has been postulated as a major cellular toxicological response to nanomaterials. It has been reported that excessive autophagy activation, induced by silica nanoparticles (SiNPs), contributes to autophagy dysfunction, whereas little is known how SiNPs trigger autophagy activatio...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2020.1763019
更新日期:2020-05-23 00:00:00
abstract::The life span of various model organisms can be extended by caloric restriction as well as by autophagy-inducing pharmacological agents. Life span-prolonging effects have also been observed in yeast cells, nematodes and flies upon the overexpression of the deacetylase Sirtuin-1. Intrigued by these observations and by ...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.6.1.10817
更新日期:2010-01-01 00:00:00
abstract::The lysosome is a key subcellular organelle that receives and degrades macromolecules from endocytic, secretory and autophagic pathways. Lysosomal function is thus critical for an efficient autophagic process. However, the molecular mechanisms mediating lysosomal function upon autophagic induction are largely unknown....
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.23965
更新日期:2013-05-01 00:00:00
abstract::ULK1 (unc-51 like autophagy activating kinase 1), the key mediator of MTORC1 signaling to autophagy, regulates early stages of autophagosome formation in response to starvation or MTORC1 inhibition. How ULK1 regulates the autophagy induction process remains elusive. Here, we identify that ATG13, a binding partner of U...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2016.1140293
更新日期:2016-01-01 00:00:00
abstract::Phagocytosis of naturally dying cells usually blocks inflammatory reactions in host cells. We have recently observed that clearance of cells dying through autophagy leads to a pro-inflammatory response in human macrophages. Investigating this response further, we found that during engulfment of MCF-7 or 293T cells und...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.7.3.14583
更新日期:2011-03-01 00:00:00
abstract::Macroautophagy is a complex process involving dynamic membrane rearrangements in which parts of the cytoplasm are sequestered within double-membrane phagophores. Upon completion, these structures mature into autophagosomes that fuse with the yeast vacuole or mammalian lysosome, leading to degradation of the cargo and ...
journal_title:Autophagy
pub_type: 社论
doi:10.4161/auto.27965
更新日期:2014-05-01 00:00:00
abstract::Autophagy is an evolutionarily conserved catabolic process that involves the engulfment of cytoplasmic contents in a closed double-membrane structure, called the autophagosome, and their subsequent delivery to the vacuole/lysosomes for degradation. Genetic screens in Saccharomyces cerevisiae have identified more than ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.21163
更新日期:2012-10-01 00:00:00
abstract::Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. W...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.7.9.16252
更新日期:2011-09-01 00:00:00
abstract::The relationship between macroautophagy/autophagy and miRNA in regulating cancer cell motility is not clearly delineated. Here, we found that induction of BECN1-dependent or -independent autophagy decreased ubiquitin-binding proteins SQSTM1/p62 and CALCOCO2/NDP52. Downregulation of SQSTM1 (but not CALCOCO2) led to a d...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2018.1501135
更新日期:2018-01-01 00:00:00
abstract::Innate immune defense against intracellular pathogens, like Salmonella, relies heavily on the autophagy machinery of the host. This response is studied intensively in epithelial cells, the target of Salmonella during gastrointestinal infections. However, little is known of the role that autophagy plays in macrophages,...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2019.1569297
更新日期:2019-05-01 00:00:00
abstract::Ticks are long-lived hematophagous arthropods and have tolerance to starvation. They can survive without food during the host-seeking period for several months to years. To understand how ticks obtain energy over a long period of non-feeding (starvation), we focused on autophagy, a crucial proteolysis system via the l...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6.4.11668
更新日期:2010-05-01 00:00:00
abstract::The flank organ of the Syrian hamster, which shows a biodynamic response to androgen stimulation, is considered a good model for studying the androgen effect on sebaceous gland and hair. This organ is susceptible to programmed cell death (PCD), a prominent feature associated with sexual organ adjustment. We have recen...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.2.7619
更新日期:2009-02-01 00:00:00
abstract::A pilot clinical trial based on nutritional modulation was designed to assess the efficacy of a one-year low-protein diet in activating autophagy in skeletal muscle of patients affected by COL6/collagen VI-related myopathies. Ullrich congenital muscular dystrophy and Bethlem myopathy are rare inherited muscle disorder...
journal_title:Autophagy
pub_type: 临床试验,杂志文章
doi:10.1080/15548627.2016.1231279
更新日期:2016-12-01 00:00:00
abstract::KRAS is the most frequently mutated oncogene in human neoplasia. Despite a large investment to understand the effects of KRAS mutation in cancer cells, the direct effects of the oncogenetic KRAS activation on immune cells remain elusive. Here, we report that extracellular KRASG12D is essential for pancreatic tumor-ass...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2020.1714209
更新日期:2020-11-01 00:00:00
abstract::The induction of autophagy appears to be tightly controlled in all eukaryotic cells. This highly conserved, degradative process is induced by a variety of signals, including nutrient deprivation, and is generally thought to be incompatible with rapid cell growth. Recent work in the budding yeast, Saccharomyces cerevis...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.2.2.2485
更新日期:2006-04-01 00:00:00