Abstract:
:Profiling antibodies to SARS-CoV-2 can help to assess potential immune response after COVID-19 disease. Luciferase IP system (LIPS) assay is a sensitive method for quantitative detection of antibodies to antigens in their native conformation. We here describe LIPS to detect antibody responses to SARS-CoV-2 spike (S) and nucleocapsid (N) proteins in COVID-19 patients. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 26 out of 26 COVID-19 patients with N antigen or with three protein fragments when combined into a single reaction. The assay correlated well with ELISA method and was specific to COVID-19 as we saw no reactivity among uninfected healthy controls. Our results show that LIPS is a rapid and measurable method to screen antibody responses against SARS-CoV-2 antigens.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Haljasmägi L,Remm A,Rumm AP,Krassohhina E,Sein H,Tamm A,Kisand K,Peterson Pdoi
10.1002/eji.202048715subject
Has Abstractpub_date
2020-08-01 00:00:00pages
1234-1236issue
8eissn
0014-2980issn
1521-4141journal_volume
50pub_type
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