Parallel RNA and DNA analysis after deep sequencing (PRDD-seq) reveals cell type-specific lineage patterns in human brain.

abstract：:Phospholamban (PLN) is an effective inhibitor of the sarco(endo)plasmic reticulum Ca(2+) ATPase (SERCA). Here, we examined PLN stability and degradation in primary cultured mouse neonatal cardiomyocytes (CMNCs) and mouse hearts using immunoblotting, molecular imaging, and [(35)S]methionine pulse-chase experiments, tog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teng AC,Miyake T,Yokoe S,Zhang L,Rezende LM Jr,Sharma P,MacLennan DH,Liu PP,Gramolini AO

更新日期：2015-06-09 00:00:00

abstract：:Gene knockout is the most powerful tool for determining gene function or permanently modifying the phenotypic characteristics of a cell. Existing methods for gene disruption are limited by their efficiency, time to completion, and/or the potential for confounding off-target effects. Here, we demonstrate a rapid single...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Santiago Y,Chan E,Liu PQ,Orlando S,Zhang L,Urnov FD,Holmes MC,Guschin D,Waite A,Miller JC,Rebar EJ,Gregory PD,Klug A,Collingwood TN

更新日期：2008-04-15 00:00:00

abstract：:In a study of juvenile hormone-regulated gene expression, we isolated an anonymous cDNA representing a message that was strongly suppressed by juvenile hormone in the fat body of the cockroach Blaberus discoidalis. The protein deduced from the cDNA sequence showed compelling resemblance in sequence to the transferrins...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamroz RC,Gasdaska JR,Bradfield JY,Law JH

更新日期：1993-02-15 00:00:00

abstract：:Using defined oligomers of IgE, our group previously studied the quantitative relationship between the aggregation of the high affinity receptors for IgE (Fc epsilonRI) and the earliest signals initiated by such aggregation: the phosphorylation of tyrosines on the receptor. Notably, at certain doses of the oligomers s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Torigoe C,Goldstein B,Wofsy C,Metzger H

更新日期：1997-02-18 00:00:00

abstract：:Bovine milk lipoprotein lipase was subjected to amino acid sequence analysis. The first 19 amino-terminal residues were Asp-Arg-Ile-Thr-Gly-Gly-Lys-Asp-Phe-Arg-Asp-Ile-Glu-Ser-Lys-Phe-Ala-Leu- Arg. In addition, reversed-phase high-performance liquid chromatography of a tryptic digest of reduced and alkylated lipase re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ben-Avram CM,Ben-Zeev O,Lee TD,Haaga K,Shively JE,Goers J,Pedersen ME,Reeve JR Jr,Schotz MC

更新日期：1986-06-01 00:00:00

abstract：:HIV-1, the cause of AIDS, is composed of four phylogenetic lineages, groups M, N, O, and P, each of which resulted from an independent cross-species transmission event of simian immunodeficiency viruses (SIVs) infecting African apes. Although groups M and N have been traced to geographically distinct chimpanzee commun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： D'arc M,Ayouba A,Esteban A,Learn GH,Boué V,Liegeois F,Etienne L,Tagg N,Leendertz FH,Boesch C,Madinda NF,Robbins MM,Gray M,Cournil A,Ooms M,Letko M,Simon VA,Sharp PM,Hahn BH,Delaporte E,Mpoudi Ngole E,Peeters M

更新日期：2015-03-17 00:00:00

abstract：:The transcription and processing of mitochondrial 21S rRNA in a petite strain of Saccharomyces cerevisiae has been examined by electron microscopic analysis of R-loop hybrids and by hybridization of labeled mitochondrial DNA probes to RNA transferred to diazobenzyloxymethyl paper. We have shown the presence of a large...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Merten S,Synenki RM,Locker J,Christianson T,Rabinowitz M

更新日期：1980-03-01 00:00:00

abstract：:The regulation of glycogen metabolism is critical for the maintenance of glucose and energy homeostasis in mammals. Glycogen synthase, the enzyme responsible for glycogen production, is regulated by multisite phosphorylation in yeast and mammals. We have previously identified PAS kinase as a physiological regulator of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilson WA,Skurat AV,Probst B,de Paoli-Roach A,Roach PJ,Rutter J

更新日期：2005-11-15 00:00:00

abstract：:Calcium, a universal second messenger, regulates diverse cellular processes in eukaryotes. Ca2+ and Ca2+/calmodulin-regulated protein phosphorylation play a pivotal role in amplifying and diversifying the action of Ca(2+)-binding domain was cloned and characterized from lily. The cDNA clone contains an open reading fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patil S,Takezawa D,Poovaiah BW

更新日期：1995-05-23 00:00:00

abstract：:The 5' flanking sequences and the intron of the mouse Hoxa-7 gene were searched for regulatory elements that can function in Drosophila. Only the intron is able to activate a lacZ fusion gene in various tissues of Drosophila embryos. This enhancer function requires a cluster of three homeodomain binding sites (HB1-ele...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haerry TE,Gehring WJ

更新日期：1996-11-26 00:00:00

abstract：:Members of the polo subfamily of protein kinases play pivotal roles in cell-cycle control and proliferation. In addition to a high degree of sequence similarity in the kinase domain, polo kinases contain a strikingly conserved motif termed "polo-box" in the noncatalytic C-terminal domain. We have previously shown that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee KS,Song S,Erikson RL

更新日期：1999-12-07 00:00:00

abstract：:Transforming growth factor (TGF)-beta plays a pivotal role in regulation of progression of cancer through effects on tumor microenvironment as well as on cancer cells. TGF-beta inhibitors have recently been shown to prevent the growth and metastasis of certain cancers. However, there may be adverse effects caused by T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kano MR,Bae Y,Iwata C,Morishita Y,Yashiro M,Oka M,Fujii T,Komuro A,Kiyono K,Kaminishi M,Hirakawa K,Ouchi Y,Nishiyama N,Kataoka K,Miyazono K

更新日期：2007-02-27 00:00:00

abstract：:Synaptic vesicles release neurotransmitter by following a process of vesicle docking and exocytosis. Although these steps are well established, it has been difficult to observe and measure these rates directly in living synapses. Here, by combining the direct imaging of single synaptic vesicles and synaptic ribbons, I...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zenisek D

更新日期：2008-03-25 00:00:00

abstract：:Adult cardiomyocytes are irreversibly postmitotic but respond to a variety of stimuli by hypertrophic growth, which is associated with an increase in cell size and protein content, organization of sarcomeres, and activation of a fetal gene program. Recently, we described a novel cardiac helicase activated by MEF2 prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu ZP,Olson EN

更新日期：2002-02-19 00:00:00

abstract：:Ants are the world's most conspicuous and important eusocial insects and their diversity, abundance, and extreme behavioral specializations make them a model system for several disciplines within the biological sciences. Here, we report the discovery of a new ant that appears to represent the sister lineage to all ext...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rabeling C,Brown JM,Verhaagh M

更新日期：2008-09-30 00:00:00

abstract：:We use quantized molecular dynamics simulations to characterize the role of enzyme vibrations in facilitating dihydrofolate reductase hydride transfer. By sampling the full ensemble of reactive trajectories, we are able to quantify and distinguish between statistical and dynamical correlations in the enzyme motion. We...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boekelheide N,Salomón-Ferrer R,Miller TF 3rd

更新日期：2011-09-27 00:00:00

abstract：:The complete nucleotide sequence of the gene for the specific glycoprotein (gp55) of the polycythemic strain of Friend spleen focus-forming virus (SFFV) was derived from the cloned SFFV DNA intermediate. The gp55 gene is present within 1.4 kilobases of the 5' side of the 3'long terminal repeat sequence. The open readi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amanuma H,Katori A,Obata M,Sagata N,Ikawa Y

更新日期：1983-07-01 00:00:00

abstract：:The matrix-2 (M2) protein from influenza A virus is a tetrameric, integral transmembrane (TM) protein that plays a vital role in viral replication by proton flux into the virus. The His37 tetrad is a pH sensor in the center of the M2 TM helix that activates the channel in response to the low endosomal pH. M2 consists ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Torabifard H,Panahi A,Brooks CL 3rd

更新日期：2020-02-18 00:00:00

abstract：:Chaperones of the Hsp70 family bind to unfolded or partially folded polypeptides to facilitate many cellular processes. ATP hydrolysis and substrate binding, the two key molecular activities of this chaperone, are modulated by the cochaperone DnaJ. By using both genetic and biochemical approaches, we provide evidence ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suh WC,Burkholder WF,Lu CZ,Zhao X,Gottesman ME,Gross CA

更新日期：1998-12-22 00:00:00

abstract：:Human urokinase cDNA clones have been identified from a cDNA library prepared from total RNA of human fibroblasts transformed by simian virus 40 [Okayama, H. & Berg, P. (1983) Mol. Cell. Biol. 3, 280-289]. Synthetic oligonucleotides, corresponding to urokinase protein sequence, were used as probes. The cloned cDNA cov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Verde P,Stoppelli MP,Galeffi P,Di Nocera P,Blasi F

更新日期：1984-08-01 00:00:00

abstract：:Alzheimer's disease (AD) is an irreversible neurodegenerative disorder that has a progression that is closely associated with oxidative stress. It has long been speculated that the reactive oxygen species (ROS) level in AD brains is much higher than that in healthy brains. However, evidence from living beings is scarc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang J,Zhang X,Yuan P,Yang J,Xu Y,Grutzendler J,Shao Y,Moore A,Ran C

更新日期：2017-11-21 00:00:00

abstract：:The effect of alpha-latrotoxin from black widow spider venom upon guinea pig cerebral cortical synaptosomes is described. Plasma membrane potential (delta psi p), in situ mitochondrial membrane potential (delta psi m), Ca2+ transport, gamma-amino[3H]butyrate release, [3H]noradrenaline release, and synaptosomal ATP wer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nicholls DG,Rugolo M,Scott IG,Meldolesi J

更新日期：1982-12-01 00:00:00

abstract：:The flavin cofactor in photoenzyme photolyase and photoreceptor cryptochrome may exist in an oxidized state and should be converted into reduced state(s) for biological functions. Such redox changes can be efficiently achieved by photoinduced electron transfer (ET) through a series of aromatic residues in the enzyme. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Z,Tan C,Guo X,Li J,Wang L,Sancar A,Zhong D

更新日期：2013-08-06 00:00:00

abstract：:One of the principles that has emerged from experimental evolutionary studies of microorganisms is that polymorphic alleles or new mutations can sometimes possess a latent potential to respond to selection in different environments, although the alleles may be functionally equivalent or disfavored under typical condit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stebbins GL,Hartl DL

更新日期：1988-07-01 00:00:00

abstract：:The quaternary structure of delta-aminolevulinic acid dehydratase (5-aminolaevulinate hydrolyase, EC 4.2.1.24) from bovine liver was examined by analytical ultracentrifugation, polyacrylamide gel electrophoresis, and electron microscopy. The molecular weights, determined by sedimentation-velocity and sedimentation-equ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu WH,Shemin D,Richards KE,Williams RC

更新日期：1974-05-01 00:00:00

abstract：:Synaptojanin 1 is a polyphosphoinositide phosphatase concentrated in presynaptic nerve terminals, where it dephosphorylates a pool of phosphatidylinositol 4,5-bisphosphate implicated in synaptic vesicle recycling. Like other proteins with a role in endocytosis, synaptojanin 1 undergoes constitutive phosphorylation in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee SY,Wenk MR,Kim Y,Nairn AC,De Camilli P

更新日期：2004-01-13 00:00:00

abstract：:Activated RAS transforming genes that encode proteins (p21s) with amino acid substitutions at positions 12, 13, or 61 have been detected in 10-20% of human neoplasms. This report describes a monoclonal antibody (DWP) raised against a synthetic peptide corresponding to amino acids 5-16 of a mutated RAS gene encoding Va...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carney WP,Petit D,Hamer P,Der CJ,Finkel T,Cooper GM,Lefebvre M,Mobtaker H,Delellis R,Tischler AS

更新日期：1986-10-01 00:00:00

abstract：:Glycoprotein E (gE) of HSV plays a key role in cell-to-cell spread and virus-induced cell fusion. Here, we report that this function of gE requires the cooperation of tegument proteins UL11, UL16, and UL21. We found that the four proteins come together with very high efficiency to form a complex in transfected cells a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han J,Chadha P,Starkey JL,Wills JW

更新日期：2012-11-27 00:00:00

abstract：:We examine how presentations of organ donation cases in the media may affect people's willingness to sign organ donation commitment cards, donate the organs of a deceased relative, support the transition to an "opt-out" policy, or donate a kidney while alive. We found that providing identifying information about the p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harel I,Kogut T,Pinchas M,Slovic P

更新日期：2017-05-16 00:00:00

abstract：:Tc1-like transposable elements from teleost fish have been phylogenetically examined to determine the mechanisms involved in their evolution and conserved domains of function. We identified two new functional domains in these elements. The first is a bipartite nuclear localization signal, indicating that transposons c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ivics Z,Izsvak Z,Minter A,Hackett PB

更新日期：1996-05-14 00:00:00