Antifungal activity of Brazilian red propolis extract and isolation of bioactive fractions by thin-layer chromatography-bioautography.

Abstract:

OBJECTIVES:This study set out to highlight the in vitro and in vivo antifungal activity of an Ethanolic Extract of Red Brazilian Propolis (EERBP) and identify bioactive fractions effective against Colletotrichum musae. METHODS:Active fractions were detected by the thin-layer chromatography-bioautography method and characterised by HPLC-MSn. RESULTS:The in vitro results showed that EERBP had strong antifungal properties againstC. musae (81 ± 1% inhibition at 1.6 g GAE L-1). Medicarpin, (3S)-vestitol and (3S)-neovestitol were the main compounds identified in the EERBP extract (45% of all detected peaks). Two isolated fractions displayed inhibition percentages of 35 ± 4 and 42 ± 1%, respectively, on C. musae mycelial growth compared to the EERBP extract. The biological activity of the two fractions displayed an additive effect. CONCLUSION:A further in vivo investigation revealed that EERBP is a potential natural alternative for controlling banana crown rot.

journal_name

Food Chem

journal_title

Food chemistry

authors

Dudoit A,Mertz C,Chillet M,Cardinault N,Brat P

doi

10.1016/j.foodchem.2020.127060

subject

Has Abstract

pub_date

2020-10-15 00:00:00

pages

127060

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(20)30922-5

journal_volume

327

pub_type

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