Abstract：
:In recent years, honey has become subject of DNA analysis due to potential risks evoked by microorganisms, allergens or genetically modified organisms. However, so far, only a few DNA extraction procedures are available, mostly time-consuming and laborious. Therefore, we developed an automated DNA extraction method from pollen in honey based on a CTAB buffer-based DNA extraction using the Maxwell 16 instrument and the Maxwell 16 FFS Nucleic Acid Extraction System, Custom-Kit. We altered several components and extraction parameters and compared the optimised method with a manual CTAB buffer-based DNA isolation method. The automated DNA extraction was faster and resulted in higher DNA yield and sufficient DNA purity. Real-time PCR results obtained after automated DNA extraction are comparable to results after manual DNA extraction. No PCR inhibition was observed. The applicability of this method was further successfully confirmed by analysis of different routine honey samples.
journal_name
Food Chemjournal_title
Food chemistryauthors
Guertler P,Eicheldinger A,Muschler P,Goerlich O,Busch Udoi
10.1016/j.foodchem.2013.10.129subject
Has Abstractpub_date
2014-04-15 00:00:00pages
302-6eissn
0308-8146issn
1873-7072pii
S0308-8146(13)01578-1journal_volume
149pub_type
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