Abstract：
:In this study, a high-throughput strategy combined with MALDI TOF/TOF-MS and Discovery Studio 2017 was developed to screen peptides with certain functions from hydrolysate. Two dominant peptides, Ile-Cys-Arg-Asp (ICRD) and Leu-Cys-Gly-Glu-Cys (LCGEC), were predicted to have antioxidant activity by Discovery Studio 2017. Then the activity in vitro of peptides had been confirmed via DPPH assay. Both two peptides decreased apoptosis induced by UVB treatment in HaCaT cells and altered Keap1/Nrf2-ARE pathway transcription. Furthermore, the antioxidant activity of LCGEC was achieved after 6-week treatment in mice via regulating the Keap1/Nrf2-ARE pathway, inhibiting the release of proinflammatory cytokines, increasing the abundance of 3-indolepropionic acid and short-chain fatty acids production in feces and modulating gut microbiota composition. This study provided two tuna roe peptides with in vitro and in vivo antioxidant activity.
journal_name
Food Chemjournal_title
Food chemistryauthors
Han J,Huang Z,Tang S,Lu C,Wan H,Zhou J,Li Y,Ming T,Jim Wang Z,Su Xdoi
10.1016/j.foodchem.2020.127094subject
Has Abstractpub_date
2020-10-15 00:00:00pages
127094eissn
0308-8146issn
1873-7072pii
S0308-8146(20)30956-0journal_volume
327pub_type
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