Abstract：
:Camellia gymnogyna Chang (CgC), a wild tea plant, was discovered on Dayao Mountain, China. However, research regarding this tea plant is limited. Our study found that CgC contains theobromine, caffeine, and theacrine, among which theobromine content was the highest (14.37-39.72 mg/g). In addition, theobromine synthase (TS) was partially purified from CgC leaves, up to 35.87-fold, with consecutive chromatography, and its molecular weight was found to be approximately 62 kDa. The optimum reaction time, pH, and temperature for theobromine synthase from 7-methylxanthine was found to be 6 h, 4, and 45 °C, respectively. TS expression at both mRNA and protein stages was higher in the first than in the fourth leaf (P < 0.05). Subcellular localization of TS indicated that it was localized in the nucleus. These results indicate that CgC can be of scientific value and could lead to efficient utilization of this rare wild tea germplasm.
journal_name
Food Chemjournal_title
Food chemistryauthors
Teng J,Yan C,Zeng W,Zhang Y,Zeng Z,Huang Ydoi
10.1016/j.foodchem.2019.125875subject
Has Abstractpub_date
2020-05-01 00:00:00pages
125875eissn
0308-8146issn
1873-7072pii
S0308-8146(19)32013-8journal_volume
311pub_type
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