Abstract:
BACKGROUND:Klebsiella pneumoniae is a public health concern because of its ability to develop multidrug resistance and hypervirulent genotypes, of those capsular types K1 and K2 cause community and nosocomial life-threatening infections. This study aimed to determine the antibiotic susceptibility patterns and genotypic traits of a collection of Klebsiella spp. isolates. Furthermore, the clonal relatedness of blaNDM producing strains was investigated. METHODS:During a 19-months surveillance study, 122 Klebsiella spp. isolates were cultured from extraintestinal specimens of patients admitted to the tertiary referral hospital in Semnan, Iran. Isolates were identified using biochemical tests and subjected to determination of phylogroups, capsular types and virulence/resistance genes content. Hypervirulent K. pneumoniae (hvKp) strains were detected genotypically, and Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR fingerprinting was used to determine the clonality of blaNDM producing strains. RESULTS:Multidrug resistant phenotype was detected in 75 (61.5%) isolates and amikacin was found as the most potent antibiotic with the susceptibility rate of 85.2%. The carbapenemase genes were detected in 45 (36.8%) strains, including 21 (17.2%) blaOXA-48, 7 (5.6%) blaNDM-1, 14 (11.4%) blaNDM-1/OXA-48 and 3 (2.4%) blaIMP- carrying strains, while 55 (45.08%) isolates showed carbapenem resistant phenotype. The first blaNDM-1 carrying strain was cultured from a sputum specimen on March 2015, while the last positive one was recovered from blood culture on September 2016. Most of the isolates (80.3%) belonged to phylogroup I, and blaNDM-1 was identified among all three phylogroups. The ERIC-PCR clustered the 101 blaNDM negative and 21 blaNDM-1 positive isolates into 25 and five clusters, respectively, and the latter group belonged to clonal complex 147 (CC147). One K1 and 15 K2 blaNDM-1 negative isolates were detected, of those three strains were identified as hvKp. Five K2 positive strains, including four blaOXA-48 producer and one hvKp sequence type 86 (ST86) were carbapenem resistant. Among carbapenem resistant isolates, CC147 strains harboured higher rates of siderophores iutA and ybtS. CONCLUSION:The present findings showed a hospital circulation of CC147 blaNDM-1 or blaNDM-1/OXA-48 producing strains, disseminated in different wards. The hvKp/ST86 strain expressing K2 capsular type and carbapenem resistant phenotype wasn't reported from Iran so far. So, it seems that we must be aware of the emergence and spread of new K. pneumoniae clones associated with resistant and hypermucoviscous phenotypes.
journal_name
Ann Clin Microbiol Antimicrobjournal_title
Annals of clinical microbiology and antimicrobialsauthors
Pajand O,Darabi N,Arab M,Ghorbani R,Bameri Z,Ebrahimi A,Hojabri Zdoi
10.1186/s12941-020-00349-zsubject
Has Abstractpub_date
2020-03-31 00:00:00pages
12issue
1issn
1476-0711pii
10.1186/s12941-020-00349-zjournal_volume
19pub_type
杂志文章abstract:BACKGROUND:Vaginitis is among the most common conditions women are seeking medical care for. Although these infections can easily be treated, the relapse rate is high. This may be due to inadequate use of the diagnostic potential. METHODS:We evaluated the misjudgement rate of the aetiology of vaginal complaints. A tot...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-5-4
更新日期:2006-02-17 00:00:00
abstract:BACKGROUND:This study aimed to evaluate in vitro susceptibility to ceftobiprole of clinical strains identified from prosthetic joint infections (PJIs) compared to that of the associations currently recommended for post-operative empirical antibiotic therapy (PEAT) (vancomycin with either cefepime, third-generation ceph...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/s12941-020-00351-5
更新日期:2020-03-21 00:00:00
abstract::Ebola virus disease (EVD) is a potentially fatal haemorrhagic disease of humans. The last and most serious outbreak of Ebola virus (EBOV) started in December 2013 in West Africa and also affected other continents. Animals such as fruit bats and non-human primates are potential sources of EBOV. This review highlights t...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,评审
doi:10.1186/s12941-015-0089-x
更新日期:2015-05-28 00:00:00
abstract:BACKGROUND:Leishmaniasis and malaria are the two most common parasitic diseases and responsible for large number of deaths per year particularly in developing countries like Pakistan. Majority of Pakistan population rely on medicinal plants due to their low socio-economic status. The present review was designed to gath...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,评审
doi:10.1186/s12941-016-0170-0
更新日期:2016-09-20 00:00:00
abstract:INTRODUCTION:Antibiotic resistance decreases success of Helicobacter pylori (Hp) eradication. Recently published results show low rate of resistance and better compliance with moxifloxacin based regiments. AIMS&METHODS: Whether 7 days moxifloxacin with lansoprasole and amoxycillin can be compared with 10 days moxifloxa...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,随机对照试验
doi:10.1186/1476-0711-9-13
更新日期:2010-04-15 00:00:00
abstract:BACKGROUND:Accurate measurement of the incidence of diarrhoeagenic E. coli in patients with diarrhoea is hindered by the current methods of detection and varies from country to country. In order to improve the diagnosis of diarrhoeagenic E. coli (DEC), we developed a set of multiplex TaqMan real-time PCRs designed to d...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-9-5
更新日期:2010-01-22 00:00:00
abstract:BACKGROUND:Diarrhoeal diseases constitute a major public health problem, particularly in the developing world, where the rate of mortality and morbidity is very high. The purpose of this study was to conduct a 2 years and 3 months study in order to determine the prevalence of five enteropathogen diarrheogenic agents in...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-6-17
更新日期:2007-12-28 00:00:00
abstract::Mycobacterium bovis BCG vaccine significantly reduces the risk of tuberculosis by 50% and continues to be used to prevent tuberculosis around the world. However, it has been shown to be ineffective in some geographical regions. The existence of different BCG strains was described more than 60 years ago, these vary in ...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-3-10
更新日期:2004-06-03 00:00:00
abstract:BACKGROUND:Antibiotic resistance is a defense mechanism, harbored by pathogens to survive under unfavorable conditions. Among several antibiotic resistant microbial consortium, Staphylococcus aureus is one of the most havoc microorganisms. Staphylococcus aureus encodes a unique enzyme 6-hydroxymethyl-7,8-dihydropterin ...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/s12941-018-0266-9
更新日期:2018-04-02 00:00:00
abstract:BACKGROUND:Drug resistance in Mycobacterium tuberculosis (MTB) is a major health issue worldwide. Recently, next-generation sequencing (NGS) technology has begun to be used to detect resistance genes of MTB. We aimed to assess the clinical usefulness of Ion S5 NGS TB research panel for detecting MTB resistance in Korea...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/s12941-018-0300-y
更新日期:2019-01-03 00:00:00
abstract:BACKGROUND:Training of infectious disease (ID) specialists is structured on classical clinical microbiology training in Turkey and ID specialists work as clinical microbiologists at the same time. Hence, this study aimed to determine the clinical skills and knowledge required by clinical microbiologists. METHODS:A cro...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-10-38
更新日期:2011-12-16 00:00:00
abstract:BACKGROUND:Multidrug resistant Pseudomonas aeruginosa harbours integrons and other mobile genetic elements such as plasmids and transposons, which easily disseminate antibiotic resistance genes among clinical strains of P. aeruginosa. METHODOLOGY:Plasmid extraction of 54 clinical isolates of P. aeruginosa was carried ...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-12-29
更新日期:2013-10-21 00:00:00
abstract:BACKGROUND:Distinct virulence factors of H. pylori have been described: the vaculating cytotoxin (vacA), the cytotoxin associated gene (cagA), the induced by contact with epithelium factor Antigen (iceA gene) and the outer membrane protein oipA. In Tunisia, there are no data regarding the pattern of H. pylori genotypes...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,多中心研究
doi:10.1186/1476-0711-9-10
更新日期:2010-03-19 00:00:00
abstract:BACKGROUND:Carbapenemase-producing Klebsiella pneumoniae (CPKP) strains have emerged as a major problem for healthcare systems. The aim of this study was to determine the circulating clones and analyze the clinical and molecular characteristics of CPKP in our hospital. METHODS:A total of 74 carbapenemase producers col...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/s12941-015-0109-x
更新日期:2016-01-19 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/s12941-015-0064-6
更新日期:2015-03-07 00:00:00
abstract:BACKGROUND:Mycetoma is a chronic mutilating disease of the skin and the underlying tissues caused by fungi or bacteria. Although recently included in the list of neglected tropical diseases by the World Health Organization, strategic control and preventive measures are yet to be outlined. Thus, it continues to pose hug...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,评审
doi:10.1186/s12941-018-0287-4
更新日期:2018-08-10 00:00:00
abstract:BACKGROUND:In 2002 we investigated an outbreak comprising 231 patients in Norway, caused by Pseudomonas aeruginosa and linked to the use of contaminated mouth swabs called Dent-O-Sept. Here we describe the extent of contamination of the swabs, and identify critical points in the production process that made the contami...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-6-3
更新日期:2007-03-13 00:00:00
abstract:BACKGROUND:The aim of the present work was to evaluate the antibacterial effect of 3,4-DHPEA-EA (methyl-4-(2-(3,4-dihydroxyphenethoxy)-2-oxoethyl)-3-formyl-2-methyl-3,4-dihydro-2H-pyran-5-carboxylate), a derivate of oleuropein, against a range of Gram-positive bacteria, including ATCC strains, food and clinical isolate...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-13-24
更新日期:2014-07-01 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/s12941-015-0102-4
更新日期:2015-09-02 00:00:00
abstract:BACKGROUND:Recently, new carbapenemases in Enterobacteriaceae strains and non-fermentative gram-negative bacilli have been reported. The New Delhi metallo-β-lactamase-1 (NDM-1) is a major problem around the world. The purpose of this article is to address the NDM-1 Klebsiella pneumoniae epidemic detected in eight cases...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/s12941-016-0118-4
更新日期:2016-02-09 00:00:00
abstract:BACKGROUND:Drug therapy is recommended in conjunction with surgery in treatment of actinomycetoma. The specific prescription depends on the type of bacteria (actinomycetoma) or fungi (eumycetoma) causing the disease and their in vitro antimicrobial susceptibility. OBJECTIVES:To investigate the antimicrobial susceptibi...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-10-24
更新日期:2011-06-06 00:00:00
abstract:BACKGROUND:Diarrheal diseases continue to be the major cause of morbidity and mortality among children under 5 years. Salmonella and Shigella specious are the major enteric pathogen causing diarrhea among children worldwide. Examination of stool sample is the most sensitive method to diagnose diarrheal disease in child...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/s12941-018-0253-1
更新日期:2018-02-01 00:00:00
abstract:BACKGROUND:Klebsiella pneumoniae is a frequent nosocomial pathogen, with the multidrug-resistant (MDR) K. pneumoniae being a major public health concern, frequently causing difficult-to-treat infections worldwide. The aim of this study was to investigate the molecular characterization of clinical MDR Klebsiella pneumon...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-13-16
更新日期:2014-05-01 00:00:00
abstract::A panel of national experts was convened by the Brazilian Infectious Diseases Society in order to organize the national recommendations for the management of zika virus infection. The focus of this document is the diagnosis, both clinical and laboratorial, and appropriate treatment of the diverse manifestations of thi...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,评审
doi:10.1186/s12941-016-0172-y
更新日期:2016-09-29 00:00:00
abstract:BACKGROUND:Previous studies report high prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) colonization among imprisoned populations. However, there are no data on that prevalence in Brazilian correctional institutions. FINDINGS:We tested 302 male prisoners for nasopharyngeal colonization with Staphyloco...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-13-25
更新日期:2014-07-02 00:00:00
abstract:BACKGROUND:The evolving epidemiology of methicillin resistant Staphylococcus aureus (MRSA) is characterized by the emergence of infections caused by non multiresistant MRSA carrying staphylococcal chromosomal cassette (SCC)mec IV or V in the healthcare settings. A molecular epidemiological analysis of non multiresistan...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-11-17
更新日期:2012-06-19 00:00:00
abstract:BACKGROUND:Escherichia coli isolates of equine faecal origin were investigated for antibiotic resistance, resistance genes and their ability to perform horizontal transfer. METHODS:In total, 264 faecal samples were collected from 138 horses in hospital and community livery premises in northwest England, yielding 296 r...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-9-12
更新日期:2010-04-07 00:00:00
abstract:BACKGROUND:Escherichia coli is the commonest cause of community and nosocomial urinary tract infection (UTI). Antibiotic treatment is usually empirical relying on susceptibility data from local surveillance studies. We therefore set out to determine levels of resistance to 8 commonly used antimicrobial agents amongst a...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-7-13
更新日期:2008-06-18 00:00:00
abstract::Stenotrophomonas maltophilia (S. maltophilia) has recently emerged as an important nosocomial pathogen. Treatment of invasive infections caused by this organism is difficult as the bacterium is frequently resistant to a wide range of commonly used antimicrobials. Trimethoprim-sulfamethoxazole (TMP - SXT) is recommende...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-5-23
更新日期:2006-09-18 00:00:00
abstract::Multi-Drug Resistant (MDR) Pseudomonas aeruginosa is one of the most important bacterial pathogens that causes infection with a high mortality rate due to resistance to different antibiotics. This bacterium prompts extensive tissue damage with varying factors of virulence, and its biofilm production causes chronic and...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,评审
doi:10.1186/s12941-020-00389-5
更新日期:2020-09-30 00:00:00