Analysis of integrons and associated gene cassettes in clinical isolates of multidrug resistant Pseudomonas aeruginosa from Southwest Nigeria.

Abstract:

BACKGROUND:Multidrug resistant Pseudomonas aeruginosa harbours integrons and other mobile genetic elements such as plasmids and transposons, which easily disseminate antibiotic resistance genes among clinical strains of P. aeruginosa. METHODOLOGY:Plasmid extraction of 54 clinical isolates of P. aeruginosa was carried out by alkaline lysis method; and plasmid size estimation was done by using E. coli V517 standard plasmid marker. Fifty-four clinical strains of P. aeruginosa were isolated from 5 hospitals in 3 Southwestern states of Nigeria between March and September 2010. Plasmid extraction of isolates was carried out by alkaline lysis method; and plasmid size estimation was done by using E. coli V517 standard plasmid marker. PCR amplification for the 3 classes of resistance integrons, and gene cassette characterization were carried out using specific primers and by sequencing of PCR products. Conjugal mating of the integron positive P. aeruginosa strains with E. coli DH5α was performed to demonstrate transferability of integrons and gene cassettes. RESULT:Agarose gel electrophoresis of plasmid DNA revealed that all the 54 P. aeruginosa harboured 1-4 plasmids with sizes ranging from 2.2 - >58 kb. Class 1 integron was identified in 31 (57%) strains; but none of them carried class 2 and class 3 integrons. High prevalence of aadA gene conferring resistance to streptomycin/spectinomycin was detected in the strains positive for class 1 integron. Sequencing of the 1.6 kb and 1.2 kb amplified band of gene cassettes revealed the presence of aadA6-orfD and aadA13 respectively. CONCLUSION:This study demonstrates the presence of plasmids and integrons harbouring resistance gene cassettes, which may collectively constitute an efficient system for dissemination of resistance genes in P. aeruginosa. Disturbingly, the rapid and unabated spread of class 1 integron-associated multidrug resistant P. aeruginosa in Southwest Nigeria may greatly hamper successful treatment of infections caused by such strains. This necessitates the establishment of functional antimicrobial resistance surveillance programmes in Nigeria.

authors

Odumosu BT,Adeniyi BA,Chandra R

doi

10.1186/1476-0711-12-29

subject

Has Abstract

pub_date

2013-10-21 00:00:00

pages

29

issn

1476-0711

pii

1476-0711-12-29

journal_volume

12

pub_type

杂志文章
  • Distribution of bla(OXA-23), ISAba , Aminoglycosides resistant genes among burned & ICU patients in Tehran and Sari, Iran.

    abstract:BACKGROUND:Multidrug resistant strains of Acinetobacter baumannii (MDR-AB) have emerged as alarming nosocomial pathogens among patients admitted to Intensive Care Unit and burned patients. The aim of this study was to determine the susceptibility of A. baumannii isolates, the carbapenems resistance patterns bla(OXA-23)...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/s12941-014-0038-0

    authors: Nasrolahei M,Zahedi B,Bahador A,Saghi H,Kholdi S,Jalalvand N,Esmaeili D

    更新日期:2014-09-25 00:00:00

  • Antimicrobial resistance in community and nosocomial Escherichia coli urinary tract isolates, London 2005-2006.

    abstract:BACKGROUND:Escherichia coli is the commonest cause of community and nosocomial urinary tract infection (UTI). Antibiotic treatment is usually empirical relying on susceptibility data from local surveillance studies. We therefore set out to determine levels of resistance to 8 commonly used antimicrobial agents amongst a...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-7-13

    authors: Bean DC,Krahe D,Wareham DW

    更新日期:2008-06-18 00:00:00

  • Ethnomedicines and anti-parasitic activities of Pakistani medicinal plants against Plasmodia and Leishmania parasites.

    abstract:BACKGROUND:Leishmaniasis and malaria are the two most common parasitic diseases and responsible for large number of deaths per year particularly in developing countries like Pakistan. Majority of Pakistan population rely on medicinal plants due to their low socio-economic status. The present review was designed to gath...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章,评审

    doi:10.1186/s12941-016-0170-0

    authors: Tariq A,Adnan M,Amber R,Pan K,Mussarat S,Shinwari ZK

    更新日期:2016-09-20 00:00:00

  • Antimicrobial resistance of H. pylori to the outcome of 10-days vs. 7-days Moxifloxacin based therapy for the eradication: a randomized controlled trial.

    abstract:INTRODUCTION:Antibiotic resistance decreases success of Helicobacter pylori (Hp) eradication. Recently published results show low rate of resistance and better compliance with moxifloxacin based regiments. AIMS&METHODS: Whether 7 days moxifloxacin with lansoprasole and amoxycillin can be compared with 10 days moxifloxa...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/1476-0711-9-13

    authors: Bago J,Majstorović K,Belosić-Halle Z,Kućisec N,Bakula V,Tomić M,Bago P,Troskot R

    更新日期:2010-04-15 00:00:00

  • Type II and type IV topoisomerase mutations in clinical isolates of Morganella morganii harbouring the qnrD gene.

    abstract:INTRODUCTION:The aim of this study was to show the emergence of the qnrD gene among fluoroquinolone-resistant Morganella morganii isolate. The occurrence of mutations in DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC,parE) genes was also investigated in this strain. METHODOLOGY:95 clinical Enterobacteria were s...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/s12941-014-0034-4

    authors: Nasri Yaiche M,Denden Rafraf I,Guo Q,Mastouri M,Aouni M,Wang M

    更新日期:2014-08-09 00:00:00

  • Performance evaluation of a rapid whole-blood immunoassay for the detection of IgG antibodies against Helicobacter pylori in daily clinical practice.

    abstract:BACKGROUND:A growing number of rapid Helicobacter pylori antibody tests are commercially available now, however, some of these tests are often used without sufficient evaluation. The aim of this study was to evaluate the performance of a commercially available rapid whole-blood immunoassay (gabControl(®) H. pylori; gab...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/s12941-016-0161-1

    authors: Enko D,Halwachs-Baumann G,Stolba R,Rössler O,Kriegshäuser G

    更新日期:2016-08-08 00:00:00

  • Antituberculosis drug resistance patterns in adults with tuberculous meningitis: results of haydarpasa-iv study.

    abstract:BACKGROUND:Tuberculous meningitis (TBM) caused by Mycobacterium tuberculosis resistant to antituberculosis drugs is an increasingly common clinical problem. This study aimed to evaluate drug resistance profiles of TBM isolates in adult patients in nine European countries involving 32 centers to provide insight into the...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章,多中心研究

    doi:10.1186/s12941-015-0107-z

    authors: Senbayrak S,Ozkutuk N,Erdem H,Johansen IS,Civljak R,Inal AS,Kayabas U,Kursun E,Elaldi N,Savic B,Simeon S,Yilmaz E,Dulovic O,Ozturk-Engin D,Ceran N,Lakatos B,Sipahi OR,Sunbul M,Yemisen M,Alabay S,Beovic B,Ulu-Kil

    更新日期:2015-11-04 00:00:00

  • Nasopharyngeal carriage of Staphylococcus aureus among imprisoned males from Brazil without exposure to healthcare: risk factors and molecular characterization.

    abstract:BACKGROUND:Previous studies report high prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) colonization among imprisoned populations. However, there are no data on that prevalence in Brazilian correctional institutions. FINDINGS:We tested 302 male prisoners for nasopharyngeal colonization with Staphyloco...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-13-25

    authors: Witzel Cde L,Fortaleza CM,de Souza CS,Riboli DF,da Cunha Mde L

    更新日期:2014-07-02 00:00:00

  • Effects of Carbapenem consumption on the prevalence of Acinetobacter infection in intensive care unit patients.

    abstract:BACKGROUND:The consumption of carbapenems has increased worldwide, together with the increase in resistant gram negative bacilli. Subsequently, the prevalence of carbapenem-resistant Acinetobacter infections has increased rapidly and become a significant problem particularly in intensive care unit patients. The aim of ...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-13-7

    authors: Ogutlu A,Guclu E,Karabay O,Utku AC,Tuna N,Yahyaoglu M

    更新日期:2014-01-09 00:00:00

  • Correlation between antibutyrylcholinesterasic and antioxidant activities of three aqueous extracts from Tunisian Rhus pentaphyllum.

    abstract::For centuries, plants have been used in traditional medicines and there has been recent interest in the chemopreventive properties of compounds derived from plants. In the present study, we investigated the antibutyrylcholinestrasic (anti-BuChE) and antioxidant (against some free radicals) activities of extracts from ...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-10-32

    authors: Ben Mansour H,Yatouji S,Mbarek S,Houas I,Delai A,Dridi D

    更新日期:2011-08-31 00:00:00

  • 3,4-DHPEA-EA from Olea Europaea L. is effective against standard and clinical isolates of Staphylococcus sp.

    abstract:BACKGROUND:The aim of the present work was to evaluate the antibacterial effect of 3,4-DHPEA-EA (methyl-4-(2-(3,4-dihydroxyphenethoxy)-2-oxoethyl)-3-formyl-2-methyl-3,4-dihydro-2H-pyran-5-carboxylate), a derivate of oleuropein, against a range of Gram-positive bacteria, including ATCC strains, food and clinical isolate...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-13-24

    authors: Bisignano C,Filocamo A,Ginestra G,Giofre' SV,Navarra M,Romeo R,Mandalari G

    更新日期:2014-07-01 00:00:00

  • An update on gastrointestinal endoscopy-associated infections and their contributing factors.

    abstract:INTRODUCTION:During clinical use, gastrointestinal endoscopes are grossly contaminated with patient's native flora. These endoscopes undergo reprocessing to prevent infectious transmission upon future use. Endoscopy-associated infections and outbreaks have been reported, with a recent focus on the transmission of multi...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章,评审

    doi:10.1186/s12941-018-0289-2

    authors: McCafferty CE,Aghajani MJ,Abi-Hanna D,Gosbell IB,Jensen SO

    更新日期:2018-10-10 00:00:00

  • Complications of peritonsillar abscess.

    abstract:BACKGROUND:The vast majority of patients with peritonsillar abscess (PTA) recover uneventfully on abscess drainage and antibiotic therapy. However, occasionally patient´s condition deteriorates as the infection spread in the upper airway mucosa, through cervical tissues, or hematogenously. The bacterial etiology of PTA...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章,评审

    doi:10.1186/s12941-020-00375-x

    authors: Klug TE,Greve T,Hentze M

    更新日期:2020-07-30 00:00:00

  • Ebola virus disease and the veterinary perspective.

    abstract::Ebola virus disease (EVD) is a potentially fatal haemorrhagic disease of humans. The last and most serious outbreak of Ebola virus (EBOV) started in December 2013 in West Africa and also affected other continents. Animals such as fruit bats and non-human primates are potential sources of EBOV. This review highlights t...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章,评审

    doi:10.1186/s12941-015-0089-x

    authors: Gumusova S,Sunbul M,Leblebicioglu H

    更新日期:2015-05-28 00:00:00

  • Antimicrobial susceptibility pattern, and associated factors of Salmonella and Shigella infections among under five children in Arba Minch, South Ethiopia.

    abstract:BACKGROUND:Diarrheal diseases continue to be the major cause of morbidity and mortality among children under 5 years. Salmonella and Shigella specious are the major enteric pathogen causing diarrhea among children worldwide. Examination of stool sample is the most sensitive method to diagnose diarrheal disease in child...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/s12941-018-0253-1

    authors: Ameya G,Tsalla T,Getu F,Getu E

    更新日期:2018-02-01 00:00:00

  • Ginger (Zingiber officinale) phytochemicals-gingerenone-A and shogaol inhibit SaHPPK: molecular docking, molecular dynamics simulations and in vitro approaches.

    abstract:BACKGROUND:Antibiotic resistance is a defense mechanism, harbored by pathogens to survive under unfavorable conditions. Among several antibiotic resistant microbial consortium, Staphylococcus aureus is one of the most havoc microorganisms. Staphylococcus aureus encodes a unique enzyme 6-hydroxymethyl-7,8-dihydropterin ...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/s12941-018-0266-9

    authors: Rampogu S,Baek A,Gajula RG,Zeb A,Bavi RS,Kumar R,Kim Y,Kwon YJ,Lee KW

    更新日期:2018-04-02 00:00:00

  • Biofilm formation and adherence characteristics of an Elizabethkingia meningoseptica isolate from Oreochromis mossambicus.

    abstract:BACKGROUND:Elizabethkingia spp. are opportunistic pathogens often found associated with intravascular device-related bacteraemias and ventilator-associated pneumonia. Their ability to exist as biofilm structures has been alluded to but not extensively investigated. METHODS:The ability of Elizabethkingia meningoseptica...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-10-16

    authors: Jacobs A,Chenia HY

    更新日期:2011-05-05 00:00:00

  • Miliary tuberculosis with no pulmonary involvement in myelodysplastic syndromes: a curable, yet rarely diagnosed, disease: case report and review of the literature.

    abstract:BACKGROUND:Although tuberculosis is not uncommon among patients with myelodysplastic syndrome (MDS), only a few reports of such patients suffering from miliary tuberculosis (MT) exist. MT often presents as a fever of unknown origin and it is a curable disease, yet fatal if left untreated. CASE PRESENTATION:We report a...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章,评审

    doi:10.1186/1476-0711-7-8

    authors: Neonakis IK,Alexandrakis MG,Gitti Z,Tsirakis G,Krambovitis E,Spandidos DA

    更新日期:2008-03-13 00:00:00

  • Generation of novel cationic antimicrobial peptides from natural non-antimicrobial sequences by acid-amide substitution.

    abstract:BACKGROUND:Cationic antimicrobial peptides (CAMPs) are well recognized to be promising as novel antimicrobial and antitumor agents. To obtain novel skeletons of CAMPs, we propose a simple strategy using acid-amide substitution (i.e. Glu→Gln, Asp→Asn) to confer net positive charge to natural non-antimicrobial sequences ...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-10-11

    authors: Ueno S,Minaba M,Nishiuchi Y,Taichi M,Tamada Y,Yamazaki T,Kato Y

    更新日期:2011-03-22 00:00:00

  • Ceftobiprole: a potential empirical post-operative monotherapy in prosthetic joint infections.

    abstract:BACKGROUND:This study aimed to evaluate in vitro susceptibility to ceftobiprole of clinical strains identified from prosthetic joint infections (PJIs) compared to that of the associations currently recommended for post-operative empirical antibiotic therapy (PEAT) (vancomycin with either cefepime, third-generation ceph...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/s12941-020-00351-5

    authors: Duployez C,Wallet F,Migaud H,Senneville E,Loiez C

    更新日期:2020-03-21 00:00:00

  • Should we use closed or open infusion containers for prevention of bloodstream infections?

    abstract:BACKGROUND:Hospitalized patients in critical care settings are at risk for bloodstream infections (BSI). Most BSIs originate from a central line (CL), and they increase length of stay, cost, and mortality. Open infusion containers may increase the risk of contamination and administration-related (CLAB) because they all...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-9-6

    authors: Rangel-Frausto MS,Higuera-Ramirez F,Martinez-Soto J,Rosenthal VD

    更新日期:2010-02-02 00:00:00

  • Invasive pulmonary aspergillosis: role of early diagnosis and surgical treatment in patients with acute leukemia.

    abstract:BACKGROUND:Aspergillus is a ubiquitous soil-dwelling fungus known to cause significant pulmonary infection in immunocompromised patients. The incidence of aspergillosis has increased during the past two decades and is a frequently lethal complication of acute leukemia patients that occurs following both chemotherapy an...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-5-17

    authors: Ali R,Ozkalemkas F,Ozcelik T,Ozkocaman V,Ozkan A,Bayram S,Ener B,Ursavas A,Ozal G,Tunali A

    更新日期:2006-07-27 00:00:00

  • Assessment of the requisites of microbiology based infectious disease training under the pressure of consultation needs.

    abstract:BACKGROUND:Training of infectious disease (ID) specialists is structured on classical clinical microbiology training in Turkey and ID specialists work as clinical microbiologists at the same time. Hence, this study aimed to determine the clinical skills and knowledge required by clinical microbiologists. METHODS:A cro...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-10-38

    authors: Erdem H,Tekin-Koruk S,Koruk I,Tozlu-Keten D,Ulu-Kilic A,Oncul O,Guner R,Birengel S,Mert G,Nayman-Alpat S,Eren-Tulek N,Demirdal T,Elaldi N,Ataman-Hatipoglu C,Yilmaz E,Mete B,Kurtaran B,Ceran N,Karabay O,Inan D,Ceng

    更新日期:2011-12-16 00:00:00

  • Successful salvage therapy with Daptomycin for osteomyelitis caused by methicillin-resistant Staphylococcus aureus in a renal transplant recipient with Fabry-Anderson disease.

    abstract::Daptomycin is licensed in adults for the management of Staphylococcus aureus methicillin-resistant infections, including bone and skin complicated infections. We describe for the first time its use in a renal transplant recipient for Fabry-Anderson Disease with right heel osteomyelitis. The patient was unresponsive to...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-11-6

    authors: Polilli E,Ursini T,Mazzotta E,Sozio F,Savini V,D'Antonio D,Barbato M,Consorte A,Parruti G

    更新日期:2012-03-11 00:00:00

  • Antimicrobial effect of farnesol, a Candida albicans quorum sensing molecule, on Paracoccidioides brasiliensis growth and morphogenesis.

    abstract:BACKGROUND:Farnesol is a sesquiterpene alcohol produced by many organisms, and also found in several essential oils. Its role as a quorum sensing molecule and as a virulence factor of Candida albicans has been well described. Studies revealed that farnesol affect the growth of a number of bacteria and fungi, pointing t...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-8-13

    authors: Derengowski LS,De-Souza-Silva C,Braz SV,Mello-De-Sousa TM,Báo SN,Kyaw CM,Silva-Pereira I

    更新日期:2009-04-29 00:00:00

  • Bed capacities and disinfection practices in hospitals in Istanbul are correlated.

    abstract:BACKGROUND:Disinfection, antisepsis and sterilization (DAS) practices are of critical importance in hospital practice. This study aims to investigate the daily DAS practices of private hospitals in Istanbul, Turkey. METHODS:The DAS practices of 155 private hospitals in Istanbul province were investigated using a quest...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/s12941-015-0073-5

    authors: Teker B,Ogutlu A,Yilmaz M,Gencer S,Karabay O

    更新日期:2015-03-19 00:00:00

  • Two or more enteropathogens are associated with diarrhoea in Mexican children.

    abstract:BACKGROUND:Diarrhoeal diseases constitute a major public health problem, particularly in the developing world, where the rate of mortality and morbidity is very high. The purpose of this study was to conduct a 2 years and 3 months study in order to determine the prevalence of five enteropathogen diarrheogenic agents in...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-6-17

    authors: Paniagua GL,Monroy E,García-González O,Alonso J,Negrete E,Vaca S

    更新日期:2007-12-28 00:00:00

  • The carbapenem-resistant Enterobacteriaceae threat is growing: NDM-1 epidemic at a training hospital in Turkey.

    abstract:BACKGROUND:Recently, new carbapenemases in Enterobacteriaceae strains and non-fermentative gram-negative bacilli have been reported. The New Delhi metallo-β-lactamase-1 (NDM-1) is a major problem around the world. The purpose of this article is to address the NDM-1 Klebsiella pneumoniae epidemic detected in eight cases...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/s12941-016-0118-4

    authors: Karabay O,Altindis M,Koroglu M,Karatuna O,Aydemir ÖA,Erdem AF

    更新日期:2016-02-09 00:00:00

  • Biological activities of extracts from Chenopodium ambrosioides Lineu and Kielmeyera neglecta Saddi.

    abstract:BACKGROUND:Chenopodium ambrosioides and Kielmeyera neglecta are plants traditionally used in Brazil to treat various infectious diseases. The study of the biological activities of these plants is of great importance for the detection of biologically active compounds. METHODS:Extracts from these plants were extracted w...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/1476-0711-11-20

    authors: Sousa ZL,de Oliveira FF,da Conceição AO,Silva LA,Rossi MH,Santos Jda S,Andrioli JL

    更新日期:2012-07-28 00:00:00

  • Hepatosplenic abscesses in an immunocompetent child with cat-scratch disease from Peru.

    abstract:BACKGROUND:Cat-scratch disease (CSD) is a zoonotic infection caused by Bartonella henselae and B. clarridgeiae. The typical manifestations of CSD include regional lymphadenitis and fever. However, CSD can have a wide variety of clinical manifestations that can lead to incorrect diagnoses and prolonged hospital stays. ...

    journal_title:Annals of clinical microbiology and antimicrobials

    pub_type: 杂志文章

    doi:10.1186/s12941-019-0322-0

    authors: Del Pozo AA,Angulo-Cruzado M,Amenero-Vega R,Álvarez-Lulichac A,Fernández-Cosavalente H,Barboza-Meca J,Rodriguez-Morales AJ

    更新日期:2019-07-15 00:00:00