Abstract:
:The microRNAs are small RNAs that regulate gene expression at the post-transcriptional level and can be involved in the onset of neurodegenerative diseases and cancer. They are emerging as possible targets for antisense-based therapy, even though the in vivo stability of miRNA analogues is still questioned. We tested the ability of peptide nucleic acids, a novel class of nucleic acid mimics, to downregulate miR-9 in vivo in an invertebrate model organism, the ascidian Ciona intestinalis, by microinjection of antisense molecules in the eggs. It is known that miR-9 is a well-conserved microRNA in bilaterians and we found that it is expressed in epidermal sensory neurons of the tail in the larva of C. intestinalis. Larvae developed from injected eggs showed a reduced differentiation of tail neurons, confirming the possibility to use peptide nucleic acid PNA to downregulate miRNA in a whole organism. By identifying putative targets of miR-9, we discuss the role of this miRNA in the development of the peripheral nervous system of ascidians.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Mercurio S,Cauteruccio S,Manenti R,Candiani S,Scarì G,Licandro E,Pennati Rdoi
10.3390/ijms21062001subject
Has Abstractpub_date
2020-03-15 00:00:00issue
6issn
1422-0067pii
ijms21062001journal_volume
21pub_type
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