Abstract：
:For the last decade, porcine epidemic diarrhea virus (PEDV) variant strains have caused severe damage to the global pig industry. Until now, no effective antivirals have been developed for the therapeutic treatment of PEDV infection. In the present study, we found that quercetin significantly suppressed PEDV infection at noncytotoxic concentrations. A molecular docking study indicated that quercetin might bind the active site and binding pocket of PEDV 3C-like protease (3CLpro). Surface plasmon resonance (SPR) analysis revealed that quercetin exhibited a binding affinity to PEDV 3CLpro. Based on the results of the fluorescence resonance energy transfer (FRET) assay, quercetin was proven to exert an inhibitory effect on PEDV 3CLpro. Since coronavirus 3CLpro is an important drug target and participates in the viral replication process, quercetin should be developed as a novel drug in the control of PEDV infection.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Li Z,Cao H,Cheng Y,Zhang X,Zeng W,Sun Y,Chen S,He Q,Han Hdoi
10.3390/ijms21218095subject
Has Abstractpub_date
2020-10-30 00:00:00issue
21issn
1422-0067pii
ijms21218095journal_volume
21pub_type
杂志文章abstract：:The aim of the experiment was to evaluate the effect of preconditioning based on changes in inspiratory oxygen fraction on endothelial function in the model of ischemia-reperfusion injury of the myocardium in the condition of cardiopulmonary bypass. The prospective randomized study included 32 rabbits divided into fou...
journal_title：International journal of molecular sciences
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