Gadolinium Chloride Restores the Function of the Gap Junctional Intercellular Communication between Hepatocytes in a Liver Injury.

Abstract:

BACKGROUND:Gadolinium chloride (GdCl3) has been reported to attenuate liver injury caused by a variety of toxicants. Gap junctional intercellular communication (GJIC) is thought to be essential in controlling liver homeostasis and pathology. Here we evaluate the effects of GdCl3 on functional GJIC and connexin expression in mouse models and primary hepatocytes. METHODS:Mice were administered GdCl3 intraperitoneally the day before a carbon tetrachloride (CCl4) injection or bile duct ligation (BDL) operation. Primary hepatocytes were treated with CCl4 or lipopolysaccharides (LPS), with or without GdCl3. A scrape loading/dye transfer assay was performed to assess the GJIC function. The expression of connexins was examined by real-time reverse transcription polymerase chain reaction (RT-PCR), western blot and immunofluorescent staining. RESULTS:CCl4 treatment or the BDL operation led to the dysfunction of GJIC and a down-regulation of Cx32 and Cx26 in injured liver. GdCl3 administration restored GJIC function between hepatocytes by facilitating the transfer of fluorescent dye from one cell into adjacent cells via GJIC, and markedly prevented the decrease of Cx32 and Cx26 in injured liver. In primary hepatocytes, CCl4 or LPS treatment induced an obvious decline of Cx32 and Cx26, whereas GdCl3 pretreatment prevented the down-regulation of connexins. In vivo GdCl3 protected hepatocytes and attenuated the liver inflammation and fibrosis in liver injury mouse models. CONCLUSION:GdCl3 administration protects functional GJIC between hepatocytes, and prevents the decrease of connexin proteins at mRNA and protein levels during liver injury, leading to the alleviation of chronic liver injury.

journal_name

Int J Mol Sci

authors

Yang L,Dong C,Tian L,Ji X,Yang L,Li L

doi

10.3390/ijms20153748

subject

Has Abstract

pub_date

2019-07-31 00:00:00

issue

15

issn

1422-0067

pii

ijms20153748

journal_volume

20

pub_type

杂志文章
  • Infections and Systemic Lupus Erythematosus: Binding or Sparring Partners?

    abstract::Extensive work on experimental animal models clearly demonstrates that infectious agents can break immunological tolerance to self-antigens and induce autoimmune disorders, mainly systemic lupus erythematosus (SLE). The establishment of a causative link between infections and autoimmunity has been largely studied in a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms160817331

    authors: Rigante D,Esposito S

    更新日期:2015-07-29 00:00:00

  • Assessment of Genetic Diversity, Population Structure, and Evolutionary Relationship of Uncharacterized Genes in a Novel Germplasm Collection of Diploid and Allotetraploid Gossypium Accessions Using EST and Genomic SSR Markers.

    abstract::This study evaluated the genetic diversity and population structures in a novel cotton germplasm collection comprising 132 diploids, including Glossypium klotzschianum and allotetraploid cotton accessions, including Glossypium barbadense, Glossypium darwinii, Glossypium tomentosum, Glossypium ekmanianum, and Glossypiu...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19082401

    authors: Ditta A,Zhou Z,Cai X,Wang X,Okubazghi KW,Shehzad M,Xu Y,Hou Y,Sajid Iqbal M,Khan MKR,Wang K,Liu F

    更新日期:2018-08-14 00:00:00

  • The Metabolic Fate of ortho-Quinones Derived from Catecholamine Metabolites.

    abstract::ortho-Quinones are produced in vivo through the oxidation of catecholic substrates by enzymes such as tyrosinase or by transition metal ions. Neuromelanin, a dark pigment present in the substantia nigra and locus coeruleus of the brain, is produced from dopamine (DA) and norepinephrine (NE) via an interaction with cys...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms17020164

    authors: Ito S,Yamanaka Y,Ojika M,Wakamatsu K

    更新日期:2016-01-27 00:00:00

  • Neonatal HDL Counteracts Placental Vascular Inflammation via S1P-S1PR1 Axis.

    abstract::Placental inflammation and dysfunction during pregnancy are associated with short- and long-term adverse outcomes for the offspring. However, the mechanisms of vascular protection at the feto-placental interface are still poorly investigated. The high-density lipoprotein (HDL) associated sphingosine-1-phosphate (S1P) ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21030789

    authors: Del Gaudio I,Hendrix S,Christoffersen C,Wadsack C

    更新日期:2020-01-25 00:00:00

  • The Safety Evaluation of Salvianolic Acid B and Ginsenoside Rg1 Combination on Mice.

    abstract::Our previous study indicated that the combination of salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1), the main components of Salvia miltiorrhizae and Panax notoginseng, improves myocardium structure and ventricular function in rats with ischemia/reperfusion injury. The present study aimed to determine the safety o...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms161226176

    authors: Zhao Q,Yang M,Deng Y,Yu H,Wang L,Teng F,Cho K,Ma H,Wu P,Li X,Wu W,Liu X,Xu F,Jiang B,Guo DA

    更新日期:2015-12-09 00:00:00

  • Dp71 Expression in Human Glioblastoma.

    abstract:BACKGROUND:Dp71 is the most abundant dystrophin (DMD) gene product in the nervous system. Mutation in the Dp71 coding region is associated with cognitive disturbances in Duchenne muscular dystrophy (DMD) patients, but the function of dystrophin Dp71 in tumor progression remains to be established. This study investigate...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20215429

    authors: Ruggieri S,De Giorgis M,Annese T,Tamma R,Notarangelo A,Marzullo A,Senetta R,Cassoni P,Notarangelo M,Ribatti D,Nico B

    更新日期:2019-10-31 00:00:00

  • Extracellular microRNAs in urologic malignancies: chances and challenges.

    abstract::Small noncoding RNAs that are 19-23 nucleotides long, known as microRNAs (miRNAs), are involved in almost all biological mechanisms during carcinogenesis. Recent studies show that miRNAs released from live cells are detectable in body fluids and may be taken up by other cells to confer cell-cell communication. These r...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms140714785

    authors: Huang X,Liang M,Dittmar R,Wang L

    更新日期:2013-07-16 00:00:00

  • Heat Shock Proteins and Autophagy Pathways in Neuroprotection: from Molecular Bases to Pharmacological Interventions.

    abstract::Neurodegenerative diseases (NDDs) such as Alzheimer's disease, Parkinson's disease and Huntington's disease (HD), amyotrophic lateral sclerosis, and prion diseases are all characterized by the accumulation of protein aggregates (amyloids) into inclusions and/or plaques. The ubiquitous presence of amyloids in NDDs sugg...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19010325

    authors: Penke B,Bogár F,Crul T,Sántha M,Tóth ME,Vígh L

    更新日期:2018-01-22 00:00:00

  • Cytokine-Mediated Dysregulation of Signaling Pathways in the Pathogenesis of Multiple Myeloma.

    abstract::Multiple myeloma (MM) is a hematologic disorder of B lymphocytes characterized by the accumulation of malignant plasma cells (PCs) in the bone marrow. The altered plasma cells overproduce abnormal monoclonal immunoglobulins and also stimulate osteoclasts. The host's immune system and microenvironment are of paramount ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21145002

    authors: Akhtar S,Ali TA,Faiyaz A,Khan OS,Raza SS,Kulinski M,Omri HE,Bhat AA,Uddin S

    更新日期:2020-07-15 00:00:00

  • Revealing and Harnessing Tumour-Associated Microglia/Macrophage Heterogeneity in Glioblastoma.

    abstract::Abstract: Cancer heterogeneity and progression are subject to complex interactions between neoplastic cells and their microenvironment, including the immune system. Although glioblastomas (GBMs) are classified as 'cold tumours' with very little lymphocyte infiltration, they can contain up to 30-40% of tumour-associate...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21030689

    authors: Pires-Afonso Y,Niclou SP,Michelucci A

    更新日期:2020-01-21 00:00:00

  • Differential Effects of a Full and Biased Ghrelin Receptor Agonist in a Mouse Kindling Model.

    abstract::The ghrelin system has received substantial recognition as a potential target for novel anti-seizure drugs. Ghrelin receptor (ghrelin-R) signaling is complex, involving Gαq/11, Gαi/o, Gα12/13, and β-arrestin pathways. In this study, we aimed to deepen our understanding regarding signaling pathways downstream the ghrel...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20102480

    authors: Buckinx A,Van Den Herrewegen Y,Pierre A,Cottone E,Ben Haj Salah K,Fehrentz JA,Kooijman R,De Bundel D,Smolders I

    更新日期:2019-05-20 00:00:00

  • Ultrasound assessment of carotid plaque echogenicity response to statin therapy: a systematic review and meta-analysis.

    abstract:OBJECTIVE:To evaluate in a systematic review and meta-analysis model the effect of statin therapy on carotid plaque echogenicity assessed by ultrasound. METHODS:We have systematically searched electronic databases (PubMed, MEDLINE, EMBASE and Cochrane Center Register) up to April, 2015, for studies evaluating the effe...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,meta分析,评审

    doi:10.3390/ijms160510734

    authors: Ibrahimi P,Jashari F,Bajraktari G,Wester P,Henein MY

    更新日期:2015-05-12 00:00:00

  • Natural products as a source for treating neglected parasitic diseases.

    abstract::Infectious diseases caused by parasites are a major threat for the entire mankind, especially in the tropics. More than 1 billion people world-wide are directly exposed to tropical parasites such as the causative agents of trypanosomiasis, leishmaniasis, schistosomiasis, lymphatic filariasis and onchocerciasis, which ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms14023395

    authors: Ndjonka D,Rapado LN,Silber AM,Liebau E,Wrenger C

    更新日期:2013-02-06 00:00:00

  • PPARβ/δ: A Key Therapeutic Target in Metabolic Disorders.

    abstract::Research in recent years on peroxisome proliferator-activated receptor (PPAR)β/δ indicates that it plays a key role in the maintenance of energy homeostasis, both at the cellular level and within the organism as a whole. PPARβ/δ activation might help prevent the development of metabolic disorders, including obesity, d...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19030913

    authors: Palomer X,Barroso E,Pizarro-Delgado J,Peña L,Botteri G,Zarei M,Aguilar D,Montori-Grau M,Vázquez-Carrera M

    更新日期:2018-03-20 00:00:00

  • AtSK11 and AtSK12 Mediate the Mild Osmotic Stress-Induced Root Growth Response in Arabidopsis.

    abstract::Although most osmotic stresses are harmful to plant growth and development, certain drought- or polyethylene glycol (PEG)-induced mild osmotic stresses promote plant root growth. The underlying regulatory mechanisms of this response remain elusive. Here, we report that the GLYCOGEN SYNTHASE KINASE 3 (GSK3) genes ARABI...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21113991

    authors: Dong L,Wang Z,Liu J,Wang X

    更新日期:2020-06-02 00:00:00

  • Development of lipid-shell and polymer core nanoparticles with water-soluble salidroside for anti-cancer therapy.

    abstract::Salidroside (Sal) is a potent antitumor drug with high water-solubility. The clinic application of Sal in cancer therapy has been significantly restricted by poor oral absorption and low tumor cell uptake. To solve this problem, lipid-shell and polymer-core nanoparticles (Sal-LPNPs) loaded with Sal were developed by a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms15033373

    authors: Fang DL,Chen Y,Xu B,Ren K,He ZY,He LL,Lei Y,Fan CM,Song XR

    更新日期:2014-02-25 00:00:00

  • Application of WES Towards Molecular Investigation of Congenital Cataracts: Identification of Novel Alleles and Genes in a Hospital-Based Cohort of South India.

    abstract::Congenital cataracts are the prime cause for irreversible blindness in children. The global incidence of congenital cataract is 2.2-13.6 per 10,000 births, with the highest prevalence in Asia. Nearly half of the congenital cataracts are of familial nature, with a predominant autosomal dominant pattern of inheritance. ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21249569

    authors: Kandaswamy DK,Prakash MVS,Graw J,Koller S,Magyar I,Tiwari A,Berger W,Santhiya ST

    更新日期:2020-12-16 00:00:00

  • In Vitro Glucuronidation of Wushanicaritin by Liver Microsomes, Intestine Microsomes and Expressed Human UDP-Glucuronosyltransferase Enzymes.

    abstract::Wushanicaritin, a natural polyphenol compound, exerts many biological activities. This study aimed to characterize wushanicaritin glucuronidation by pooled human liver microsomes (HLM), human intestine microsomes and individual uridine diphosphate-glucuronosyltransferase (UGT) enzyme. Glucuronidation rates were determ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18091983

    authors: Hong X,Zheng Y,Qin Z,Wu B,Dai Y,Gao H,Yao Z,Gonzalez FJ,Yao X

    更新日期:2017-09-19 00:00:00

  • Modular Chimeric Antigen Receptor Systems for Universal CAR T Cell Retargeting.

    abstract::The engineering of T cells through expression of chimeric antigen receptors (CARs) against tumor-associated antigens (TAAs) has shown significant potential for use as an anti-cancer therapeutic. The development of strategies for flexible and modular CAR T systems is accelerating, allowing for multiple antigen targetin...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21197222

    authors: Sutherland AR,Owens MN,Geyer CR

    更新日期:2020-09-30 00:00:00

  • Circulating Tumour Cell Expression of Immune Markers as Prognostic and Therapeutic Biomarkers in Head and Neck Squamous Cell Carcinoma: A Systematic Review and Meta-Analysis.

    abstract::Rates of loco-regional recurrence and distant metastasis remain high among head and neck squamous cell carcinoma (HNSCC) patients, despite advancing cancer treatment modalities and therapeutic agents. One area that has generated considerable interest is the immune landscape of the tumour, heralding a wave of immune ch...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21218229

    authors: Payne K,Pugh M,Brooks J,Batis N,Taylor G,Nankivell P,Mehanna H

    更新日期:2020-11-03 00:00:00

  • Genetically Engineered-MSC Therapies for Non-unions, Delayed Unions and Critical-size Bone Defects.

    abstract::The normal bone regeneration process is a complex and coordinated series of events involving different cell types and molecules. However, this process is impaired in critical-size/large bone defects, with non-unions or delayed unions remaining a major clinical problem. Novel strategies are needed to aid the current th...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20143430

    authors: Freitas J,Santos SG,Gonçalves RM,Teixeira JH,Barbosa MA,Almeida MI

    更新日期:2019-07-12 00:00:00

  • Protective Effects of Cardamom in Isoproterenol-Induced Myocardial Infarction in Rats.

    abstract::Cardamom is a popular spice that has been commonly used in cuisines for flavor since ancient times. It has copious health benefits such as improving digestion, stimulating metabolism, and exhibits antioxidant and anti-inflammatory effects. The current study investigated the effect of cardamom on hemodynamic, biochemic...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms161126040

    authors: Goyal SN,Sharma C,Mahajan UB,Patil CR,Agrawal YO,Kumari S,Arya DS,Ojha S

    更新日期:2015-11-17 00:00:00

  • Mast Cells in Cardiovascular Disease: From Bench to Bedside.

    abstract::Mast cells are pluripotent leukocytes that reside in the mucosa and connective tissue. Recent studies show an increased prevalence of cardiovascular disease among patients with mastocytosis, which is a hematological disease that is characterized by the accumulation of mast cells due to clonal proliferation. This assoc...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20143395

    authors: Hermans M,Lennep JRV,van Daele P,Bot I

    更新日期:2019-07-10 00:00:00

  • Drosophila Models of Sporadic Parkinson's Disease.

    abstract::Parkinson's disease (PD) is the most common cause of movement disorders and is characterized by the progressive loss of dopaminergic neurons in the substantia nigra. It is increasingly recognized as a complex group of disorders presenting widely heterogeneous symptoms and pathology. With the exception of the rare mono...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19113343

    authors: Nagoshi E

    更新日期:2018-10-26 00:00:00

  • Molecular mechanisms of (R,R)ZX-5 on NO synthesis and its anti-angiogenic effect.

    abstract::(R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Analysis of Western bl...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms13032717

    authors: Pan L,Hu JL,Wang WJ,Zhang XJ,Wei J,Liu ZD,Zhang YH,Xu HM

    更新日期:2012-01-01 00:00:00

  • An Ameliorated Prediction of Drug-Target Interactions Based on Multi-Scale Discrete Wavelet Transform and Network Features.

    abstract::The prediction of drug-target interactions (DTIs) via computational technology plays a crucial role in reducing the experimental cost. A variety of state-of-the-art methods have been proposed to improve the accuracy of DTI predictions. In this paper, we propose a kind of drug-target interactions predictor adopting mul...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18081781

    authors: Shen C,Ding Y,Tang J,Xu X,Guo F

    更新日期:2017-08-16 00:00:00

  • Galangin Reduces the Loss of Dopaminergic Neurons in an LPS-Evoked Model of Parkinson's Disease in Rats.

    abstract::Parkinson's disease (PD) is caused by the loss of dopaminergic (DA) neurons in the midbrain substantia nigra (SN). Neuroinflammation, which is marked by microglial activation, plays a very important role in the pathogenesis of PD. Pro-inflammatory mediators produced by activated microglia could damage DA neurons. Henc...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19010012

    authors: Chen G,Liu J,Jiang L,Ran X,He D,Li Y,Huang B,Wang W,Fu S

    更新日期:2017-12-21 00:00:00

  • CSPG4 as Target for CAR-T-Cell Therapy of Various Tumor Entities-Merits and Challenges.

    abstract::Targeting cancer cells using chimeric-antigen-receptor (CAR-)T cells has propelled adoptive T-cell therapy (ATT) to the next level. A plentitude of durable complete responses using CD19-specific CAR-T cells in patients suffering from various lymphoid malignancies resulted in the approval by the food and drug administr...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20235942

    authors: Harrer DC,Dörrie J,Schaft N

    更新日期:2019-11-26 00:00:00

  • Essential Role of Endothelial MCPIP in Vascular Integrity and Post-Ischemic Remodeling.

    abstract::MCP-1-induced protein (MCPIP, also known as Zc3h12a or Regnase-1), a newly identified suppressor of cytokine signaling, is expressed in endothelial cells (ECs). To investigate the role of endothelial MCPIP in vascular homeostasis and function, we deleted the MCPIP gene specifically in ECs using the Cre-LoxP system. EC...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20010172

    authors: Jin Z,Niu J,Kapoor N,Liang J,Becerra E,Kolattukudy PE

    更新日期:2019-01-05 00:00:00

  • Investigation of the neuroprotective impact of nimodipine on Neuro2a cells by means of a surgery-like stress model.

    abstract::Nimodipine is well characterized for the management of SAH (subarachnoid hemorrhage) and has been shown to promote a better outcome and less DIND (delayed ischemic neurological deficits). In rat experiments, enhanced axonal sprouting and higher survival of motoneurons was demonstrated after cutting or crushing the fac...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms151018453

    authors: Herzfeld E,Strauss C,Simmermacher S,Bork K,Horstkorte R,Dehghani F,Scheller C

    更新日期:2014-10-14 00:00:00