Abstract：
:We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa-arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow-derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post-crosslinking gelation method was revealed to be cytocompatible and showed temperature-dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Akimoto AM,Niitsu EH,Nagase K,Okano T,Kanazawa H,Yoshida Rdoi
10.3390/ijms19041253subject
Has Abstractpub_date
2018-04-21 00:00:00issue
4issn
1422-0067pii
ijms19041253journal_volume
19pub_type
杂志文章abstract：:Triazophos is a widely used organophosphorous insecticide that has potentially adverse effects to organisms. In the present study, a high-affinity single-chain variable fragment (scFv) antibody with specific lambda light chain was developed for residue monitoring. First, the specific variable regions were correctly am...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17060823
更新日期：2016-06-07 00:00:00
abstract：:Neurodegenerative disorders affect around one billion people worldwide. They can arise from a combination of genomic, epigenomic, metabolic, and environmental factors. Aging is the leading risk factor for most chronic illnesses of old age, including Alzheimer's and Parkinson's diseases. A progressive neurodegenerative...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20030719
更新日期：2019-02-08 00:00:00
abstract：:During plant-pathogen interactions, plants have to relocate their resources including energy to defend invading organisms; as a result, plant growth and development are usually reduced. Arabidopsis signal responsive1 (AtSR1) has been documented as a negative regulator of plant immune responses and could serve as a pos...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19061764
更新日期：2018-06-14 00:00:00
abstract：:Nuclear receptors (NRs) play a key role in regulating virtually all body functions, thus maintaining a healthy operating body with all its complex systems. Recently, gut microbiota emerged as major factor contributing to the health of the whole organism. Enteric bacteria have multiple ways to influence their host and ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19082210
更新日期：2018-07-28 00:00:00
abstract：:Manganese (Mn) is an important trace element present in human body, which acts as an enzyme co-factor or activator in various metabolic reactions. While essential in trace amounts, excess levels of Mn in human brain can produce neurotoxicity, including idiopathic Parkinson's disease (PD)-like extrapyramidal manganism ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020300
更新日期：2017-01-30 00:00:00
abstract：:Early prediction of xenobiotic metabolism is essential for drug discovery and development. As the most important human drug-metabolizing enzyme, cytochrome P450 3A4 has a large active cavity and metabolizes a broad spectrum of substrates. The poor substrate specificity of CYP3A4 makes it a huge challenge to predict th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160714677
更新日期：2015-06-30 00:00:00
abstract：:In this work, Au-Bi(2)Te(3) nanocomposite thermoelectric film with a hierarchical sub-micron antireflection quasi-periodic structure was synthesized via a low-temperature chemical route using Troides helena (Linnaeus) forewing (T_FW) as the biomimetic template. This method combines chemosynthesis with biomimetic techn...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2015-06-03 00:00:00
abstract：:Phosphatidylcholine (PC) translocation into mucus of the intestine was shown to occur via a paracellular transport across the apical/lateral tight junction (TJ) barrier. In case this could also be operative in biliary epithelial cells, this may have implication for the pathogenesis of primary sclerosing cholangitis (P...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20164034
更新日期：2019-08-19 00:00:00
abstract：:Type 1 diabetes mellitus (T1D) is a chronic autoimmune disease resulting in the destruction of insulin producing β-cells of the pancreas, with consequent insulin deficiency and excessive glucose production. Hyperglycemia results in increased levels of reactive oxygen species (ROS) and nitrogen species (RNS) with conse...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20102441
更新日期：2019-05-17 00:00:00
abstract：:Calcium carbonate, especially with nanostructure, has been considered as a good candidate material for bone regeneration due to its excellent biodegradability and osteoconductivity. In this study, rod-like calcium carbonate nanoparticles (Rod-CC NPs) with desired water dispersibility were achieved with the regulation ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17050639
更新日期：2016-05-06 00:00:00
abstract：:Adiponectin is the adipokine associated with insulin sensitization, reducing liver gluconeogenesis, and increasing fatty acid oxidation and glucose uptake. Adiponectin is present in the kidneys, mainly in the arterial endothelium and smooth muscle cells, as well as in the capillary endothelium, and might be considered...
journal_title：International journal of molecular sciences
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更新日期：2020-12-09 00:00:00
abstract：:GABARAP (γ-aminobutyric acid type A receptor-associated protein) and its paralogues GABARAPL1 and GABARAPL2 comprise a subfamily of autophagy-related Atg8 proteins. They are studied extensively regarding their roles during autophagy. Originally, however, especially GABARAPL2 was discovered to be involved in intra-Golg...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22010085
更新日期：2020-12-23 00:00:00
abstract：:DEFECTIVE IN ANTHER DEHISCENCE 1 (DAD1), a phospholipase A1, utilizes galactolipids (18:3) to generate α-linolenic acid (ALA) in the initial step of jasmonic acid (JA) biosynthesis in Arabidopsis thaliana. In this study, we isolated the JcDAD1 gene, an ortholog of Arabidopsis DAD1 in Jatropha curcas, and found that it...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21238923
更新日期：2020-11-24 00:00:00
abstract：:Emodin, an active constituent of oriental herbs, is widely used to treat allergy, inflammation, and other symptoms. This study provides the scientific basis for the anti-inflammasome effects of emodin on both in vitro and in vivo experimental models. Bone marrow-derived macrophages were used to study the effects of em...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16048102
更新日期：2015-04-10 00:00:00
abstract：:Persistent cell shrinkage, called apoptotic volume decrease (AVD), is a pivotal event of apoptosis. Activation of the volume-sensitive outwardly rectifying Cl(-) channel (VSOR) is involved in the AVD induction. On the other hand, activation of the MAP kinase (MAPK) cascade is also known to play a critical role in apop...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13079363
更新日期：2012-01-01 00:00:00
abstract：:α-Synuclein is a naturally unfolded protein which easily aggregates and forms toxic inclusions and deposits. It is associated with several neurodegenerative diseases, including Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA). These diseases, called synucleinopathies, have o...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082801
更新日期：2020-04-17 00:00:00
abstract：:Epigenetic alterations by promoter DNA hypermethylation and gene silencing in cancer have been reported over the past few decades. DNA hypermethylation has great potential to serve as a screening marker, a prognostic marker, and a therapeutic surveillance marker in cancer clinics. Some bodily fluids, such as stool or ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18040735
更新日期：2017-03-30 00:00:00
abstract：:Despite improvements in diagnosis and treatment, breast cancer is still the most common cancer type among non-smoking females. TGF-β can inhibit breast cancer development by inducing cell cycle arrest in both, cancer cells and, as part of a senescence program in normal human mammary epithelial cells (HMEC). Moreover, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18071574
更新日期：2017-07-20 00:00:00
abstract：:Biblical references aside, restoring vision to the blind has proven to be a major technical challenge. In recent years, considerable advances have been made towards this end, especially when retinal degeneration underlies the vision loss such as occurs with retinitis pigmentosa. Under these conditions, optogenetic the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21020522
更新日期：2020-01-14 00:00:00
abstract：:Oxidative stress and the resulting damage to DNA are inevitable consequence of endogenous physiological processes further amplified by cellular responses to environmental exposures. If left unrepaired, oxidative DNA lesions can block essential processes such as transcription and replication or can induce mutations. Em...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20020364
更新日期：2019-01-16 00:00:00
abstract：:It has been shown that activation of adenosine monophosphate-activated protein kinase (AMPK) suppresses proliferation of a variety of tumor cells as well as nonmalignant cells. In this study, we used post-transcriptional gene silencing with small interfering RNA (siRNA) to specifically examine the effect of AMPK on pu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17060844
更新日期：2016-05-31 00:00:00
abstract：:Cryopreservation of immature testicular tissue (ITT) prior to chemo/radiotherapy is now ethically accepted and is currently the only way to preserve fertility of prepubertal boys about to undergo cancer therapies. So far, three-dimensional culture of testicular cells isolated from prepubertal human testicular tissue w...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20215476
更新日期：2019-11-03 00:00:00
abstract：:Ceramide and diacylglycerol are linked to insulin resistance in rodents, but in humans the data are inconsistent. Insulin resistance is frequently observed with aging, but the role of ceramide and diacylglycerol is not clarified. Training improves metabolic health and, therefore, we aimed to elucidate the influence of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051240
更新日期：2019-03-12 00:00:00
abstract：:Candida auris is a potential multidrug-resistant pathogen able to persist on indwelling devices as a biofilm, which serve as a source of catheter-associated infections. Neosartorya fischeri antifungal protein 2 (NFAP2) is a cysteine-rich, cationic protein with potent anti-Candida activity. We studied the in vitro acti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020771
更新日期：2021-01-14 00:00:00
abstract：:Chitosan, a naturally occurring polysaccharide with abundant resources, has been extensively exploited for various biomedical applications, typically as wound dressings owing to its unique biocompatibility, good biodegradability and excellent antibacterial properties. In this work, composite nanofibrous membranes of c...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms11093529
更新日期：2010-09-21 00:00:00
abstract：:Melanoma is a lethal tumor because of its severe metastatic potential, and serine/threonine-protein kinase B-raf inhibitors (BRAFi) are used in patients harboring BRAF-mutation. Unfortunately, BRAFi induce resistance. Therefore, we tested the activity of onconase (ONC), a cytotoxic RNase variant, against BRAFi-resista...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20235980
更新日期：2019-11-27 00:00:00
abstract：:The present study investigates the photochemical properties of potential photosensitizers for photodynamic therapy, namely four commercial heptamethinecyanine dyes (IR125, IR780, IR813, IR820). Spectroscopic studies were made by means of laser induced fluorescence and laser flash photolysis in order to obtain fluoresc...
journal_title：International journal of molecular sciences
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doi：10.3390/ijms140918557
更新日期：2013-09-09 00:00:00
abstract：:Epithelial ovarian cancer (EOC) is the leading cause of death among gynecological malignancies. Despite surgery and chemotherapy, 5-years survival rates have improved only modestly over the past few decades remaining at 45% for advanced stages. Therefore, novel therapies are urgently needed. The presence of tumor-infi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20122927
更新日期：2019-06-15 00:00:00
abstract：:The drought is a crucial environmental factor that determines yielding of many crop species, e.g., Fabaceae, which are a source of valuable proteins for food and feed. Herein, we focused on the events accompanying drought-induced activation of flower abscission zone (AZ)-the structure responsible for flower detachment...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20153731
更新日期：2019-07-31 00:00:00
abstract：:Thirteen polyphenolics were isolated from fresh pods of Caesalpinia pulcherrima using various methods of column chromatography. The structures of these polyphenolics were elucidated as gallic acid (1), methyl gallate (2), 6-O-galloyl-d-glucoside (3), methyl 6-O-galloyl-β-d-glucoside (4), methyl 3,6-di-O-galloyl-α-d-gl...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13056073
更新日期：2012-01-01 00:00:00
