Abstract：
AIMS:Both, vitamin D3 and human periodontal ligament cells (hPDLCs) possess immunosuppressive properties, but their combined effect on immune cells has never been investigated. Here, we analysed the impact of vitamin D3 on the immunosuppressive properties of hPDLCs towards CD4+ T lymphocytes. MATERIAL AND METHODS:Allogenic CD4+ T lymphocytes were activated by phytohemagglutinin either in monoculture or co-culture with hPDLCs, in the presence or absence of IFN-γ and 1,25(OH)2 D3 . After 5 days, CD4+ T-lymphocyte proliferation, CD4+ CD25+ FoxP3+ regulatory T lymphocytes (Tregs ) proportion and IL-10, TGF-β1 and IL-17A production were analysed. RESULTS:In monoculture, 1,25(OH)2 D3 suppressed CD4+ T-lymphocyte proliferation, increased the percentage of CD4+ FoxP3+ CD25+ FoxP3+ Tregs and enhanced IL-10 and TGF-β1 production. In the presence of IFN-γ treated hPDLCs, 1,25(OH)2 D3 significantly increased CD4+ T-lymphocyte proliferation and decreased the percentage of CD4+ CD25+ FoxP3+ Tregs . IL-10 and IL-17A expression was significantly diminished by 1,25(OH)2 D3 , whereas TGF-β1 was slightly increased. The effects of 1,25(OH)2 D3 in co-culture were reversed by inhibition of indoleamine-2,3-dioxygenase-1, prostaglandin-endoperoxide synthase and programmed cell death 1 ligand 1. 1,25(OH)2 D3 also suppressed the expression of these proteins in hPDLCs. CONCLUSION:Effects of vitamin D3 on CD4+ T lymphocyte are modified by hPDLCs depending on the microenvironment.
journal_name
J Clin Periodontoljournal_title
Journal of clinical periodontologyauthors
Behm C,Blufstein A,Gahn J,Kubin B,Moritz A,Rausch-Fan X,Andrukhov Odoi
10.1111/jcpe.13283subject
Has Abstractpub_date
2020-06-01 00:00:00pages
689-701issue
6eissn
0303-6979issn
1600-051Xjournal_volume
47pub_type
杂志文章abstract：BACKGROUND:B-cells are prominent immune cells in established periodontitis lesions. Tumour necrosis factor superfamily (TNFSF) cytokines play roles in supporting B-cell function as well as bone re-modelling. The influence of smoking on factors that support B-cell function in periodontitis remains unclear. AIM:To inves...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12134
更新日期：2013-09-01 00:00:00
abstract：:Periodontitis is an inflammatory disease of the periodontium which is characterized by a progressive destruction of the tissues supporting the tooth. Its primary etiology is an ill-defined series of microbial infections which may be composed of only some of the more than 300 species of bacteria currently recognized in...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,评审
doi：10.1111/j.1600-051x.1986.tb01485.x
更新日期：1986-05-01 00:00:00
abstract：:A number of compounds have been added to toothpastes to inhibit plaque regrowth. The inclusion of cationic antiseptics, such as chlorhexidine, poses formulation difficulties because of interactions with other ingredients particularly anionic detergents. More recently, Triclosan/zinc citrate formulations have been show...
journal_title：Journal of clinical periodontology
pub_type： 临床试验,杂志文章
doi：10.1111/j.1600-051x.1989.tb00008.x
更新日期：1989-07-01 00:00:00
abstract：AIM:Our aim was to measure the association of maternal periodontitis with low birth weight (LBW), pre-term LBW, and intra-uterine growth restriction. MATERIAL AND METHODS:An inclusive case-control design including subjects examined for periodontitis through attachment loss, information on perinatal outcomes and genera...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2006.01012.x
更新日期：2007-01-01 00:00:00
abstract：:Healing events following nonsurgical periodontal therapy were studied, comparing the effect of a single initial instrumentation to the effect of 3 instrumentations, each separated by 3 months. Incisors, cuspids and premolars with periodontal pockets up to 11 mm deep in 13 patients were treated by plaque control and su...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1984.tb00839.x
更新日期：1984-02-01 00:00:00
abstract：AIM:To compare polymerase chain reaction (PCR) with subsequent reverse hybridization (micro-IDent test) and checkerboard DNA-DNA hybridization for the identification of 13 bacterial species in subgingival plaque samples. MATERIAL AND METHODS:Subgingival plaque samples were taken using paper points and curettes from tw...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2009.01434.x
更新日期：2009-08-01 00:00:00
abstract：PURPOSE:The objective of the present study was to compare inter-proximal fill and papilla height between different embrasures. MATERIAL & METHODS:One hundred and fifty non-smoking consecutive patients (mean age 54, range 32-73; 63 males and 87 females) without periodontal disease were selected in a multidisciplinary p...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12116
更新日期：2013-07-01 00:00:00
abstract：OBJECTIVES:To study the short-term microbiological changes following full-mouth compared with quadrant wise scaling and root planing (FMRP and QRP) as well as long-term effects. METHOD:Twenty patients with chronic periodontitis were randomized into a test group treated in two sessions with subgingival scaling and root...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-051X.2006.01035.x
更新日期：2007-02-01 00:00:00
abstract：:In the present study, the effect of supra and subgingival plaque debridement on the dynamics of the subgingival microflora in deep pockets was investigated. 8 adult periodontitis patients participated in the study. In each patient, 4 clinically diseased sites were investigated microbiologically by phase contrast micro...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1988.tb01004.x
更新日期：1988-02-01 00:00:00
abstract：:This report will focus attention on the diagnostic and therapeutic difficulties involved in a patient who presented with a gingival papillary-verrucous lesion, which subsequently developed into a squamous cell carcinoma. ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1987.tb01515.x
更新日期：1987-01-01 00:00:00
abstract：AIM:The aim of this study was to determine whether the combined connective tissue graft (CTG) with injectable platelet-rich fibrin (i-PRF) with coronally advanced flap (CAF) improved root coverage of deep Miller Class I or II gingival recessions compared with CTG alone with CAF. MATERIAL AND METHODS:Seventy-two patien...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,随机对照试验
doi：10.1111/jcpe.13193
更新日期：2020-01-01 00:00:00
abstract：:Calculus formation on tooth surfaces is analogous to the formation of lime and scale deposits in plumbing. Magnetic water devices have been shown to significantly reduce scale deposits in industry; therefore an oral irrigator with a magnetic water device may have a similar effect on calculus. To test this hypothesis, ...
journal_title：Journal of clinical periodontology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1111/j.1600-051x.1993.tb00366.x
更新日期：1993-05-01 00:00:00
abstract：AIM:This case-control study investigates the relationship of stress and anxiety with periodontal clinical characteristics. METHOD:Seventy-nine selected patients (mean age 46.8+/-8 years) were assigned to three groups in accordance with their levels of probing pocket depth (PPD): control group (PPD< or =3 mm, n=22), te...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.2003.10271.x
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND:Soft tissue myxoma of the oral cavity is rare. Only three cases of myxoma of gingiva have been reported in the literature. We present a case of soft tissue myxoma arising from the left maxillary adherent gingiva in a 42-year-old male [correction of female] patient. METHODS:Histological examination showed sp...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2005.00837.x
更新日期：2006-01-01 00:00:00
abstract：AIM:To evaluate the impact of two non-surgical periodontal treatment modalities on metabolic and periodontal clinical parameters in subjects with type 2 diabetes mellitus (T2DM) and poor glycaemic control and chronic periodontitis. MATERIAL AND METHODS:A randomized controlled clinical trial was conducted. Ninety-three...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,随机对照试验
doi：10.1111/jcpe.12991
更新日期：2018-09-01 00:00:00
abstract：AIM:To investigate the prevalence of tetM, tetQ, nim and bla(TEM) antimicrobial resistance genes in subgingival and tongue samples of Greek subjects. MATERIALS AND METHODS:Fifty-four subjects participated in the present study. Participants each contributed with one pooled subgingival sample from the mesiobuccal surfac...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2009.01425.x
更新日期：2009-07-01 00:00:00
abstract：OBJECTIVE:This study evaluates whether or not, among other factors, membrane-coverage of antrostomy defects improves implant survival in sinus augmentation procedures. MATERIALS AND METHODS:We performed a two-arm and split-mouth randomized controlled clinical trial on 104 and 5 patients respectively. In the two-arm st...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,随机对照试验
doi：10.1111/jcpe.12153
更新日期：2013-11-01 00:00:00
abstract：:The present paper outlines some of the difficulties encountered in the search for the etiologic agents of destructive periodontal diseases. These include technical problems such as acquiring an appropriate microbial sample, as well as difficulties in the dispersion, cultivation and identification of isolates in that s...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1987.tb01520.x
更新日期：1987-11-01 00:00:00
abstract：OBJECTIVES:This study investigates the effect of a range of potential risk factors on the severity of gingival overgrowth in transplant patients medicated with ciclosporin in the absence of any calcium channel blockers. MATERIALS AND METHODS:One hundred dentate solid organ transplants medicated with ciclosporin (but n...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2005.00657.x
更新日期：2005-03-01 00:00:00
abstract：OBJECTIVES:Removal of dental plaque is an essential element of periodontal treatment. However, there have also been studies of the effects of the mechanical stimulation provided by toothbrushing on gingival host-defense mechanisms. The aim of the study was to evaluate the effects of toothbrushing on gingival fibroblast...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.2002.290901.x
更新日期：2002-09-01 00:00:00
abstract：:Periodontal disease indicators were evaluated according to the periodontal treatment need system (PTNS) in random samples of 35-year-old citizens of Oslo in 1973 and 1984. The study indicated that although periodontal disease was a common finding in both samples, there was a significant reduction in score C (indicatin...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1990.tb00021.x
更新日期：1990-04-01 00:00:00
abstract：AIM:To test whether or not the use of a volume-stable collagen matrix (VCMX) results in soft tissue volume increase at implant sites non-inferior to an autogenous subepithelial connective tissue graft (SCTG). METHODS:In 20 patients, soft tissue augmentation at implant sites was performed using VCMX or SCTG. Casts obta...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,随机对照试验
doi：10.1111/jcpe.12697
更新日期：2017-04-01 00:00:00
abstract：:The effect of ascorbic acid megadoses on gingival clinical parameters and vitamin content of blood and gingival tissue was studied. 10 nondeficient individuals, carefully matched according to age, periodontal status, and oral hygiene level, were divided into 2 groups: one received 250 mg q.i.d. of ascorbic acid and th...
journal_title：Journal of clinical periodontology
pub_type： 临床试验,杂志文章
doi：10.1111/j.1600-051x.1984.tb01319.x
更新日期：1984-03-01 00:00:00
abstract：OBJECTIVES:The increased plaque formation observed in gingival inflammation is not fully understood. Receptor proteins in the dental pellicle might influence bacterial adhesion and subsequent plaque formation. The purpose of the present study was to examine proteins and microorganisms in dental biofilms, at healthy and...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.2002.290609.x
更新日期：2002-06-01 00:00:00
abstract：AIM:To study the effect of the dose and type of calcium channel blockers (CCBs) on the risk of gingival hyperplasia and to quantify this association. METHODS:The study was conducted within the Integrated Primary Care Information Project in The Netherlands. A nested case-control study was designed within a cohort of al...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2010.01574.x
更新日期：2010-07-01 00:00:00
abstract：OBJECTIVES:Evaluation of the clinical effect of topical subgingival application of doxycycline gel adjunctively to scaling and root planing (SRP) at furcation sites during supportive periodontal therapy (SPT). MATERIAL AND METHODS:In 39 SPT patients exhibiting at least four pockets > or m with bleeding on probing, SRP...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-051X.2009.01414.x
更新日期：2009-06-01 00:00:00
abstract：:The purpose of this study was to evaluate the sensitivity of 4 clinical techniques used in detecting early plaque during a period of dietary sucrose restriction and during a period of sucrose supplementation. The accumulation of plaque during experimental periods of 3 h, 6 h and 18 h was recorded in a group of 38 dent...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1986.tb00862.x
更新日期：1986-08-01 00:00:00
abstract：OBJECTIVES:The aim of this case-control study was to ascertain if women who experienced a preterm (or premature) birth had any differences in periodontal disease severity compared with women who delivered at term. MATERIAL AND METHODS:Subjects were recruited postpartum. Case subjects delivered a baby before 37 weeks o...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2004.00598.x
更新日期：2005-01-01 00:00:00
abstract：:A review of the literature was made, focusing on experimental designs used in clinical studies for assessment of the efficacy of interdental cleaning aids with regard to plaque removal and the prevention of caries and gingivitis. After careful consideration of the pros and cons of published procedures, an experimental...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,评审
doi：10.1111/j.1600-051x.1993.tb00695.x
更新日期：1993-11-01 00:00:00
abstract：BACKGROUND:Little is known about the periodontal status of patients with Sjögren's Syndrome (SS), a chronic inflammatory autoimmune disease characterized by xerophthalmia and xerostomia. The aim of the present study was to evaluate whether the periodontal status of SS patients, in terms of clinical and microbiological ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.2002.290202.x
更新日期：2002-02-01 00:00:00
