Abstract:
AIM:To investigate the prevalence of tetM, tetQ, nim and bla(TEM) antimicrobial resistance genes in subgingival and tongue samples of Greek subjects. MATERIALS AND METHODS:Fifty-four subjects participated in the present study. Participants each contributed with one pooled subgingival sample from the mesiobuccal surface of the four first molars and one sample from the tongue. Samples were analysed using polymerase chain reaction for tetM, tetQ, nim and bla(TEM) genes using the primers and conditions described previously. Subjects were stratified according to periodontal status (health, gingivitis or periodontitis). Intake of any antibiotic for medical or dental reasons during the previous 12 months was also recorded (self-reported). Comparisons within and between groups were performed by applying non-parametric tests (z-test with Bonferroni corrections). RESULTS:A high prevalence of tetM, tetQ and bla(TEM) genes was detected in both tongue and subgingival samples (48.1-82.2%). No differences were observed across genes between periodontally healthy, gingivitis or periodontitis cases, and no statistical correlation was observed between the presence of the bla(TEM) gene and the intake of beta-lactams during the last 12 months (Fisher's exact test, p>0.05). CONCLUSIONS:Findings from the present study suggest a high prevalence of tetM, tetQ and bla(TEM), but not nim resistance genes in subgingival and tongue samples from Greek subjects.
journal_name
J Clin Periodontoljournal_title
Journal of clinical periodontologyauthors
Ioannidis I,Sakellari D,Spala A,Arsenakis M,Konstantinidis Adoi
10.1111/j.1600-051X.2009.01425.xsubject
Has Abstractpub_date
2009-07-01 00:00:00pages
569-74issue
7eissn
0303-6979issn
1600-051Xpii
CPE1425journal_volume
36pub_type
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journal_title:Journal of clinical periodontology
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doi:10.1111/j.1600-051x.1994.tb00272.x
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journal_title:Journal of clinical periodontology
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journal_title:Journal of clinical periodontology
pub_type: 杂志文章
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journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1992.tb00454.x
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journal_title:Journal of clinical periodontology
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journal_title:Journal of clinical periodontology
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doi:10.1111/j.1600-051x.1994.tb00288.x
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journal_title:Journal of clinical periodontology
pub_type: 杂志文章,随机对照试验
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更新日期:2019-11-01 00:00:00
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journal_title:Journal of clinical periodontology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2009-03-01 00:00:00
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journal_title:Journal of clinical periodontology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2019-09-01 00:00:00
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journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1034/j.1600-051x.2000.027012926.x
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journal_title:Journal of clinical periodontology
pub_type: 杂志文章,随机对照试验
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更新日期:2012-05-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2019-12-01 00:00:00
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journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1982.tb01231.x
更新日期:1982-03-01 00:00:00
abstract::The present study describes an in vivo model for the collection of the subgingival pellicle adsorbed to tooth surface, and the identification of some serum proteins within this layer. Clean dentin slabs were prepared from freshly extracted teeth, and then placed subgingivally for 2 h. The dentin slabs with their adsor...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1991.tb00439.x
更新日期:1991-05-01 00:00:00
abstract::Actinobacillus actinomycetemcomitans was isolated from periodontal pockets in a patient suffering from prepubertal periodontitis. Electron microscopy revealed 3 different groups of bacteriophages in filtrates of subgingival plaque from all the active periodontal lesions. Phage infected A. actinomycetemcomitans in this...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1987.tb00974.x
更新日期:1987-04-01 00:00:00
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journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051X.2005.00710.x
更新日期:2005-06-01 00:00:00