Abstract:
OBJECTIVES:This research aimed to investigate the relative level of Runt-related transcription factor 2 (RUNX2) in giant cell tumor of bone (GCTB). Through the histopathological similarities between osteoporosis and GCTB, the biological functions of exogenous RUNXS were demonstrated in GCTB cell lines. This generated awareness of the molecular mechanism of the biogenesis and metastasis of GCTB, as well as showing the pathways and processes involved in this study. This research also expected to provide hints for the clinical treatment of patients with GCTB, to release the tumor burden and reduce the recurrence rate and metastasis of patients with this condition. METHODS:The expression of RUNX2 in the tumors was verified by Western Blot, qRT-PCR and immunohistochemistry, compared with the normal tissues' adjacent tumors. Subsequently, the plasmids expressing RUNX2 were constructed, amplified and transfected into the 0404 cell line through transfection kits (0.4, 0.8, 1.6, 2.4 ng/μl). After that, the proliferation, migration, invasion, cellular viability and apoptosis of 0404 cell lines were examined by EDU assay, wound healing assay, transwell assay, annexin v staining, and CCK8 assay, respectively. RESULTS:The messenger RNA (mRNA) level of RUNX2 in tumors was over 100 folds more than the normal tissues. The protein level of tumors upregulated 8.32(±4.41) folds relatively. After the transfection of RUNX2 overexpressed plasmids into the 0404 cell line, the mRNA level of RUNX2 increased approximately 530.11(±24.87), 1117.96(±77.68), 2835.09(±45.22) and 4781.51(±79.37) folds respectively, and the protein level was upregulated about 4.12(±1.15), 16.73(±1.63), 21.53(±2.41) and 23.39(±0.85) folds respectively. The proliferation of 0404 cells was inhibited by 2.13(±1.02)% of 1.6 ng/μl group and 3.03(±1.76)% of 2.4 ng/μl group. And the migration was inhibited about 45.56(±6.13)%, 50.79(±5.27)%, 63.15(±8.62)% and 93.90(±3.65)% respectively. The invasion was decreased about 14.49(±5.4)%, 37.02(±6.52)%, 42.24(±2.59)% and 48.97(±10.61)% respectively. Meanwhile, FITC Annexin V/PI apoptosis assay demonstrated that RUNX2 plasmids could promote apoptosis rate around 4.15(±0.27)%, 5.07(±0.27)%, 7.61(±0.45)% and 11.32(±1.02)% respectively, and CCK8 proved these plasmids could weaken cellular viability in a concentration-dependent manner with the time passing. CONCLUSIONS:RUNX2 is highly expressed in giant cell tumors of bone. The RUNX2 overexpressed plasmids we constructed could be successfully transfected into 0404 cell line. Far more importantly, the exogenous RUNX2 can seriously block the biological functions of 0404 cell line in a concentration-dependent manner, including proliferation, translocation, invasion, cellular viability, and apoptosis. Meanwhile, the mechanism was hypothesized and discussed in the article.
journal_name
Orthop Surgjournal_title
Orthopaedic surgeryauthors
Jin YH,Zhang J,Zhu H,Fan GT,Zhou GXdoi
10.1111/os.12568subject
Has Abstractpub_date
2020-04-01 00:00:00pages
668-678issue
2eissn
1757-7853issn
1757-7861journal_volume
12pub_type
杂志文章abstract:OBJECTIVE:To investigate the methods for, and clinical outcome of, the operative treatment of thoracolumbar fractures through an approach via the paravertebral muscle (PVM). METHODS:From June 2005 to August 2006, 62 patients, comprising 48 men and 14 women with an average age of 45.2 years (range, 21-58) with thoracol...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1757-7861.2009.00032.x
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVES:A series of constant anatomical structures were used as guide targets for screw placement to improve the accuracy of cortical screw placement and reduce surgical injury and fluoroscopy radiation. The most commonly used angles and distances between the cortical bone screw insertion point and the lateral margi...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12775
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:To evaluate the functional outcomes of arthroscopic anatomical repair of anterior talofibular ligament (ATFL) in the treatment of chronic lateral ankle instability (CLAI) during medium- and long-term follow-up. METHODS:From September 2014 to August 2017, the data of 37 patients (23 males, 14 females; 12 left...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12651
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVE:To investigate the biological functions of long noncoding RNA-H19 (H19) in the pathogenesis of disuse osteoporosis (DOP). METHODS:Fifty-four male Sprague Dawley (SD) rats were randomly divided into three groups: baseline control (BC, 6), age-matched control (AC, 24), and hindlimb unloading (HLU, 24). The rat...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12321
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:To investigate a stem-adjusted preparation of the femur with a kinked reamer and to determine whether this approach results in higher primary stability of a kinked stem than straight reaming of the intramedullary canal. METHODS:Ten cementless stems with a kinked design were implanted in synthetic femurs afte...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12490
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVE:To evaluate the efficacy and safety of hip replacement and intramedullary nails for treating unstable intertrochanteric fractures in elderly patients. METHODS:Randomized clinical trials (RCTs) to compare hip replacement with intramedullary nail in the management of elderly patients with unstable intertrochan...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,meta分析
doi:10.1111/os.12532
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:To evaluate the clinical effect of the minimally invasive transforaminal lumbar interbody fusion combined with posterolateral fusion and unilateral fixation using a tubular retractor in the management of degenerative lumbar disease. METHODS:A retrospective analysis was conducted to analyze the clinical outco...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12345
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:To present the use of an intermediate dorsal neurocutaneous flap for the reconstruction of defects on the distal foot. METHODS:From September 2016 to October 2018, five patients (mean age at operation 33.8 years; range, 7-70 years; female/male = 2/3) with skin defects on one of their feet caused by road-traf...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12623
更新日期:2020-04-01 00:00:00
abstract::Pure tibiotalar dislocation without an associated fracture is an extremely rare injury. We present three cases of closed posteromedial tibiotalar dislocation without any associated fractures to the foot, ankle, or leg. All patients were treated conservatively with immediate closed reduction under general or local anae...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12049
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVE:To summarize the indications and the clinical effects of a transfibular neck osteotomy approach and a combined anterolateral and posterolateral approach in the treatment of fractures of the lateral tibial plateau involving the posterolateral column. METHODS:Eleven patients with lateral tibial plateau fractur...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12544
更新日期:2019-12-01 00:00:00
abstract::The objective of this systematic review and meta-analysis was to evaluate the efficacy and safety of i.v. tranexamic acid (TXA) in simultaneous bilateral total knee arthroplasty (TKA). Potentially relevant published reports were identified from the following electronic databases: Medline, PubMed, Embase, ScienceDirect...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,meta分析,评审
doi:10.1111/os.12256
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVE:To quantitatively describe the stress of the ligamentum flavum (LF) using the finite element method and to compare the stress at different parts of the healthy LF. METHODS:Based on the high resolution computed tomography imaging data of a healthy 22-year-old man, three-dimensional nonlinear L4-5 lumbar finit...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12675
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVES:Occipitocervical fusion (OCF) is an effective treatment for instability of occipitocervical junction (OCJ). The occipital condyle screw serves as a novel surgical technique for occipitocervical fixation. However, the intraoperative procedures for the occipital condyle screw technique have relied on surgeons'...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12700
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:To investigate the efficiency of anterior decompression on the proximal-type cervical spondylotic amyotrophy patients. METHODS:This was a retrospective analysis. From January 2014 to November 2017, 21 patients with proximal-type cervical spondylotic amyotrophy (CSA) underwent anterior decompression. There we...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12654
更新日期:2020-06-01 00:00:00
abstract::The authors describe a hybrid technique that involves a combination of open decompression and posterior lumbar interbody fusion (PLIF) and percutaneously inserted pedicle screws. This technique allows performance of PLIF and decompression via a midline incision and approach without compromising operative time and visu...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12042
更新日期:2013-05-01 00:00:00
abstract::Diaphyseal defects in long bones of extremities following segmental resection are very common in the clinic, and reconstruction remains a great challenge. Although there are many treatments for diaphyseal defects at present, reconstruction with an intercalary endoprosthesis may be an optimal method. We demonstrate a s...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12391
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:Acute hematogenous infection is a devastating complication that can occur after total knee arthroplasty (TKA). The best strategies for management of this infection remain controversial. Two-stage revision has been well described as the gold standard for the management of chronic late infections. However, ther...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12297
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the clinical outcomes of anterior and posterior instrumentation with different debridement and graft fusion methods for multi-level contiguous thoracic spinal tuberculosis. METHODS:We retrospectively evaluated 81 patients with multi-level contiguous thoracic spinal tuberculosis who underwent ante...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12288
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:To describe the technique of primary repair of medial collateral ligament (MCL) insufficiency using a screw and rectangular spiked washer in a case series of 14 patients. METHODS:Fourteen patients undergoing MCL repair by a screw and rectangular spiked washer during TKA between March 2018 and March 2019 were...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12818
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:Giant cell tumors of bone (GCTs) are bone destructive neoplasms, the bone resorption being mediated by osteoclasts. Given that microRNAs are crucially involved in tumorigenesis and the modulation of cell fate and behavior, they are promising candidates for regulation of osteoclastogenesis. However, no reliabl...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12231
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVE:The aim of this study is to identify the common microorganisms causing PJI as well as the drug-resistant spectrum for each microorganism, to help orthopaedic surgeons to choose appropriate antibiotics. METHOD:One hundred and sixty patients who suffered from failure of primary or revision total hip or knee ar...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12394
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:We create a new S1 cortical screw trajectory technique using 3D reconstruction and the finite element (FE) method to provide a more reliable theoretical basis for clinical practices and to advance internal fixation technology for treatment of lumbosacral degenerative diseases. METHODS:This retrospective stud...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12624
更新日期:2020-02-01 00:00:00
abstract::Because current therapies have not always been successful and effective, the possibility of regenerating the nucleus pulposus (NP) through a tissue-engineered construct offers a novel therapeutic possibility for symptomatic degenerative disc diseases (DDDs). However, more research is necessary to identify the optimal ...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12020
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the mid-term outcomes of transoral atlantoaxial reduction plate (TARP) internal fixation for the treatment of irreducible atlantoaxial dislocation. METHODS:From April 2003 to April 2005, 31 patients with irreducible atlantoaxial dislocation were treated with TARP internal fixation. The average ag...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2010.00077.x
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVE:To investigate the compensatory mechanism of maintaining the sagittal balance in degenerative lumbar scoliosis patients with different pelvic incidence (PI). METHODS:This was a retrospective imaging observation study. Patients in our department with degenerative lumbar scoliosis between 2017 and 2019 were re...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12805
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:At present, cephalomedullary nail is the most frequently used implant in the management of intertrochanteric fractures around the world. The implant design and fixation techniques of the cephalomedullary nail have been continuously improved to ensure uncomplicated bone union during the past decade. However, a...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12828
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:To evaluate the results of treatment of osteonecrosis of the femoral head by structural augmentation through a routine core decompression procedure combined with insertion of cannulated bone screws incorporating autogenous bone graft and biomaterial containing decalcified bone matrix. METHODS:From February 2...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2008.00008.x
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVE:To examine the results of an anular closure device for prevention of lumbar disc reherniation in daily routine practice. METHODS:Fifty patients with large anular defects were treated with limited discectomy and a bone-anchored anular closure device. The device physically occludes the defect in the anulus fib...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12495
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:To evaluate the clinical efficacy of internal fixation versus hemiarthroplasty in the treatment of unstable intertrochanteric fractures in the elderly. METHODS:A search was conducted in PubMed, Web of Science, Embase, and Cochrane Library databases up to April 2020. The present study compared internal fixati...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,评审
doi:10.1111/os.12736
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVE:To investigate the changes of inflammatory factors and bone metabolism markers in postmenopausal women with H-type hypertension and to assess the relationship between them. METHODS:Postmenopausal women who were diagnosed with osteoporosis were selected as observation objects. Participants were divided into t...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12567
更新日期:2019-12-01 00:00:00