Abstract:
OBJECTIVE:Giant cell tumors of bone (GCTs) are bone destructive neoplasms, the bone resorption being mediated by osteoclasts. Given that microRNAs are crucially involved in tumorigenesis and the modulation of cell fate and behavior, they are promising candidates for regulation of osteoclastogenesis. However, no reliable miRNAs profile for GCT is available. Our study aimed to evaluate osteoclastogenesis-related miRNA expression in GCTs of Chinese patients. METHODS:From January 2013 to December 2014, 11 patients with GCTs were treated in our department and grouped into a GCT group. A control group comprising four patients with benign tumors of the iliac bone was established. The diagnoses were initially established by imaging examinations and intraoperative frozen sections and later confirmed by standard histologic examination. The GCT group (five male and six female patients) were aged from 17 to 61 years (mean, 32.9 years; SD, 12.8 years). Six patients with GCT underwent intralesional curettage surgery and the other five wide resection. According to Campanacci grading, four patients had Grade I tumors, three Grade II, and three Grade III. The average age of the control group was 28.75 years (SD, 14.24 years); all of them were diagnosed as having benign tumors and underwent iliac grafting. The morphology of the excised tissue was evaluated by examining standardized hematoxylin and eosin (HE) stained paraffin-embedded samples. In all, three osteoclastogenesis-related RNAs and 20 microRNAs (miRNAs) were extracted from the patients. The strength of expression was assessed by quantitative reverse transcription polymerase chain reaction (PCR ) and the results assessed by a Student's t test. RESULTS:Examination of HE stained sections revealed that the higher the Campanacci grade, the more numerous and bigger the osteoclasts (P < 0.05). PCR results indicated large amounts of osteoclast-related mRNA (cathepsin K, tartrate-resistant acid phosphatase and matrix metalloproteinase9) in GCTs (P < 0.05). Expression of six miRNAs was significantly weaker in the GCT than the control group (P < 0.05). The expression of has-mir-16-5p and has-let-7a-5p was correlated with Campanacci grade in the GCT patients (P = 0.009 and 0.034, respectively). The expression of these two miRNAs may indicate severity of bone destruction. CONCLUSION:Overall, the clinical utility of six novel miRNA markers for GCTs was demonstrated. Of these, strength of expression of hsa-mir-16-5p and hsa-let-7a-5p may indicate the grade of bone resorption.
journal_name
Orthop Surgjournal_title
Orthopaedic surgeryauthors
Qin S,He NB,Yan HL,Dong Ydoi
10.1111/os.12231subject
Has Abstractpub_date
2016-05-01 00:00:00pages
212-9issue
2eissn
1757-7853issn
1757-7861journal_volume
8pub_type
杂志文章abstract:OBJECTIVE:Lumbar spinal stenosis is a medical condition characterized by the narrowing of the spinal canal as a consequence of bone and soft tissue degeneration, including disc herniation, facet and ligamentum flavum hypertrophy, and osteophyte formation. The percutaneous transforaminal endoscopic discectomy (PTED) tec...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12559
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVE:We analyzed chondrosarcomas in long bones to assess whether Grade I and II chondrosarcomas should both be grouped as low grade malignant tumors (musculoskeletal Tumor Society Stage I or Stage II), and to explore rational surgical treatment for Grade I and II chondrosarcomas. METHODS:We retrospectively review...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2009.00020.x
更新日期:2009-05-01 00:00:00
abstract::To observe the morphology character of the anterior epidural space at the L4-5 disc level and to provide an anatomical basis for safely and accurately performing a percutaneous endoscopic lumbar discectomy (PELD). Fifty-five cases with L5 S1 lumbar disc herniation were included in this study, and cases with L4-5 disea...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12325
更新日期:2017-05-01 00:00:00
abstract::A 66-year-old woman had sustained crush injury 3 hours prior to her presentation to our hospital. The diagnosis was defined as lateral tibial plateau fracture of the right knee (Schatzker III). Supine position was set up and a pad was put under the affected hip. After sterilization of the surgical field the sterilized...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12205
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:To examine the results of an anular closure device for prevention of lumbar disc reherniation in daily routine practice. METHODS:Fifty patients with large anular defects were treated with limited discectomy and a bone-anchored anular closure device. The device physically occludes the defect in the anulus fib...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12495
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:The aim of the present study was to evaluate the effect of patellar denervation (PD) in preventing anterior knee pain (AKP) and improving knee function after total knee arthroplasty (TKA) without patellar resurfacing, and to help surgeons decide whether or not to use PD in TKA. METHODS:The electronic databas...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12815
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:To investigate delay in diagnosis by both patients and doctors, and to evaluate its effect on outcomes of high-grade sarcoma of bone in a single-referral oncological center. METHODS:Fifty-four patients with osteosarcoma, 29 with Ewing sarcoma and 19 with chondrosarcoma were enrolled in this retrospective stu...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12239
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVE:The prevention and treatment of the common complication of cerebrospinal fluid leakage (CSFL) during anterior approach cervical spine surgery for severe ossification of the posterior longitudinal ligament (OPLL) is documented. METHOD:A retrospective analysis of 47 patients with severe cervical OPLL aged 39 t...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12007
更新日期:2012-11-01 00:00:00
abstract:OBJECTIVE:Relatively few studies have reported on the morphology of the supracondylar femur, which is a fundamental factor affecting prosthetic reconstruction. The objectives of the present study were to measure the morphological parameters of the supracondylar femur, to classify the supracondylar femur, and to provide...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12443
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVE:Acute hematogenous infection is a devastating complication that can occur after total knee arthroplasty (TKA). The best strategies for management of this infection remain controversial. Two-stage revision has been well described as the gold standard for the management of chronic late infections. However, ther...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12297
更新日期:2016-11-01 00:00:00
abstract::Stem cell research has been a popular topic in the past few decades. This review aims to discuss factors that help regulate, induce, and enhance mesenchymal stem cell (MSC) differentiation into osteoblasts for bone regeneration. The factors analyzed include bone morphogenic protein (BMP), transforming growth factor β ...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,评审
doi:10.1111/os.12304
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the safety and effectiveness of osteotomy adjacent to the articular surface of the metatarsal head combined with basal opening wedge osteotomy for severe hallux valgus. METHODS:The double osteotomy procedure was carried out in 56 patients (72 feet) with severe hallux valgus deformity, with an ave...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12505
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVE:To evaluate the clinical efficacy of internal fixation versus hemiarthroplasty in the treatment of unstable intertrochanteric fractures in the elderly. METHODS:A search was conducted in PubMed, Web of Science, Embase, and Cochrane Library databases up to April 2020. The present study compared internal fixati...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,评审
doi:10.1111/os.12736
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVE:To evaluate the results of treatment of osteonecrosis of the femoral head by structural augmentation through a routine core decompression procedure combined with insertion of cannulated bone screws incorporating autogenous bone graft and biomaterial containing decalcified bone matrix. METHODS:From February 2...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2008.00008.x
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVE:To assess the benefits of use of a tourniquet in one limb in patients undergoing simultaneous bilateral total knee arthroplasty (TKA). METHODS:A prospective randomized trial was designed to evaluate the outcomes of unilateral tourniquet use during simultaneous bilateral TKA. A total of 52 (36 women and 16 me...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,随机对照试验
doi:10.1111/os.12329
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:The arcuate foramen is a complete or partial bony bridge over the vertebral artery groove of atlas. The mechanism of the arcuate foramen is not clearly understood. Omission of the arcuate foramen sometimes causes lethal iatrogenic injury during spinal surgery. CASE PRESENTATION:We describe a patient who was...
journal_title:Orthopaedic surgery
pub_type:
doi:10.1111/os.12868
更新日期:2020-12-03 00:00:00
abstract::Diaphyseal defects in long bones of extremities following segmental resection are very common in the clinic, and reconstruction remains a great challenge. Although there are many treatments for diaphyseal defects at present, reconstruction with an intercalary endoprosthesis may be an optimal method. We demonstrate a s...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12391
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:To investigate the outcomes of total knee arthroplasty (TKA) in patients with a prior femoral or tibial fracture, and identify the risk factors for surgical site complications and reoperations. METHODS:Seventy-one TKAs performed in 71 patients with a prior tibial or femoral fracture between January 2005 and ...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12610
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVE:To compare the superior-level facet joint violations (FJV) between robot-assisted (RA) percutaneous pedicle screw placement and conventional open fluoroscopic-guided (FG) pedicle screw placement in a prospective cohort study. METHODS:This was a prospective cohort study without randomization. One-hundred pati...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12534
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:To investigate the efficiency of anterior decompression on the proximal-type cervical spondylotic amyotrophy patients. METHODS:This was a retrospective analysis. From January 2014 to November 2017, 21 patients with proximal-type cervical spondylotic amyotrophy (CSA) underwent anterior decompression. There we...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12654
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:To compare the efficacy and safety of the postoperative long-term effect of the treatment of single-level cervical spondylosis through anterior cervical discectomy and fusion (ACDF) and artificial cervical disc replacement (ACDR). METHODS:This is a retrospective contrastive study, which was conducted for the...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12634
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:To present the use of an intermediate dorsal neurocutaneous flap for the reconstruction of defects on the distal foot. METHODS:From September 2016 to October 2018, five patients (mean age at operation 33.8 years; range, 7-70 years; female/male = 2/3) with skin defects on one of their feet caused by road-traf...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12623
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVE:To investigate a stem-adjusted preparation of the femur with a kinked reamer and to determine whether this approach results in higher primary stability of a kinked stem than straight reaming of the intramedullary canal. METHODS:Ten cementless stems with a kinked design were implanted in synthetic femurs afte...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12490
更新日期:2019-08-01 00:00:00
abstract::Herein is described cortical bone trajectory (CBT), a new path for pedicle screw insertion for lumbar vertebral fusion. Because the points of insertion are under the end of the inferior articular process, and because the screws are inserted toward the lateral side, there is less soft tissue development than with the c...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12122
更新日期:2014-08-01 00:00:00
abstract:OBJECTIVE:To discuss the methods, timing and clinical outcomes of surgical treatment for open pilon fractures. METHODS:From April 2003 to July 2008, 28 patients with open pilon fractures were treated. All had type C fractures according to the Arbeitsgemeinschaft für osteosynthesefragen-Association for the Study of Int...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2010.00113.x
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:To introduce a microsurgical suture technique for repair of dural tear under posterior lumbar disk scope. METHODS:Micro endoscopic discectomy was performed on a 26-year-old male under local anesthesia. During the operation, an irregular tear of about 1.0 cm was inadvertently made in the dura.The cauda equina...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2011.00135.x
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVE:To analyze the approach and feasibility of one-stage anterior release and reduction with posterior fusion for irreducible atlantoaxial dislocation. METHODS:Ten male and 6 female patients, with an average age of 36 years, including 13 patients with old trauma, 2 with rheumatoid disease, and 1 with os odontoid...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2009.00049.x
更新日期:2009-11-01 00:00:00
abstract:OBJECTIVES:This research aimed to investigate the relative level of Runt-related transcription factor 2 (RUNX2) in giant cell tumor of bone (GCTB). Through the histopathological similarities between osteoporosis and GCTB, the biological functions of exogenous RUNXS were demonstrated in GCTB cell lines. This generated a...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12568
更新日期:2020-04-01 00:00:00
abstract::Historically, a simple approach centered on palliation was applicable to the majority of patients with metastatic spinal disease. With advances in diagnosis and treatment, a more complicated algorithm has devolved requiring a multidisciplinary approach with institutional commitment and support. We performed a database...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,评审
doi:10.1111/os.12334
更新日期:2017-05-01 00:00:00
abstract::Because current therapies have not always been successful and effective, the possibility of regenerating the nucleus pulposus (NP) through a tissue-engineered construct offers a novel therapeutic possibility for symptomatic degenerative disc diseases (DDDs). However, more research is necessary to identify the optimal ...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12020
更新日期:2013-02-01 00:00:00