Abstract:
OBJECTIVE:To investigate the biological functions of long noncoding RNA-H19 (H19) in the pathogenesis of disuse osteoporosis (DOP). METHODS:Fifty-four male Sprague Dawley (SD) rats were randomly divided into three groups: baseline control (BC, 6), age-matched control (AC, 24), and hindlimb unloading (HLU, 24). The rats in the BC group were sacrificed at the beginning of the experiment, while the AC and HLU rats were sacrificed at different times (7, 14, 21 and 28 days after HLU). The DOP model was verified by micro-CT scan, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to quantify the expression of osteogenic genes (OPG, RunX2 and OPG). Gene sequencing and bioinformatic analysis were performed to find H19 target genes and the associated signaling pathway, which were first verified on tissue samples. Further verification was performed by knocking down the H19 and related gene in rat osteoblast cell line (UMR106 cell). Then, the changes of associated signaling pathway and osteogenic function were examined to confirm the prediction of the bioinformatic analysis. RESULTS:Micro-CT scans and quantitative real-time polymerase chain reaction (qRT-PCR) tests showed progressively deteriorated trabecular bone and decreased level of osteogenic genes in the metaphysis of distal femur during HLU, indicating the successful establishment of a DOP model. According to RNA sequencing, 1351 mRNA and 464 lncRNA were abnormally expressed in response to mechanical unloading, in which the H19 decreased 2.86 fold in HLU rats. There were 1426 mRNA predicted to be the target genes of H19, and KEGG pathway analysis suggested that Wnt signaling pathway (Wnt signaling) was the top pathway responsible for these target genes. In the Wnt-associated genes targeted by H19, 11 were differentially expressed between HLU and AC rats, among which Dkk4 increased 2.44 fold in HLU rats when compared to normal controls. These results of sequencing and bioinformatic analysis were confirmed by the low expression of H19, overexpression of Dkk4 and inhibited Wnt signaling observed in DOP rats. Subsequent in vitro cell assay further demonstrated that knockdown of H19 led to upregulation of Dkk4, and inhibition of Wnt signaling and osteogenic function in UMR106 cell. These effects can be greatly reversed after application of knocking down Dkk4. CONCLUSION:Our findings demonstrated that low expression of H19, induced by mechanical unloading, leads to development of DOP through inhibition of Wnt signaling by promoting Dkk4 expression.
journal_name
Orthop Surgjournal_title
Orthopaedic surgeryauthors
Li B,Liu J,Zhao J,Ma JX,Jia HB,Zhang Y,Xing GS,Ma XLdoi
10.1111/os.12321subject
Has Abstractpub_date
2017-08-01 00:00:00pages
319-327issue
3eissn
1757-7853issn
1757-7861journal_volume
9pub_type
杂志文章abstract::Because current therapies have not always been successful and effective, the possibility of regenerating the nucleus pulposus (NP) through a tissue-engineered construct offers a novel therapeutic possibility for symptomatic degenerative disc diseases (DDDs). However, more research is necessary to identify the optimal ...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12020
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:To compare the superior-level facet joint violations (FJV) between robot-assisted (RA) percutaneous pedicle screw placement and conventional open fluoroscopic-guided (FG) pedicle screw placement in a prospective cohort study. METHODS:This was a prospective cohort study without randomization. One-hundred pati...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12534
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:Total knee arthroplasty (TKA) is one of the most universal and effective means for treating terminal stage osteoarthritis (OA) of knee. Accurate intramedullary guide of femur is the basis for the distal femoral cuts. Determining the surgical transepicondylar axis (sTEA) is the key to reconstruction of the fem...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,随机对照试验
doi:10.1111/os.12619
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVE:Although contributions to sagittal alignment have been characterized for anterior, posterior and transforaminal lumbar interbody fusion, sagittal alignment after lateral transpsoas interbody fusion (LTIF) has not yet been characterized. This study examined the ability of LTIF to restore lumbar lordosis and id...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2012.00175.x
更新日期:2012-05-01 00:00:00
abstract::Herein is described cortical bone trajectory (CBT), a new path for pedicle screw insertion for lumbar vertebral fusion. Because the points of insertion are under the end of the inferior articular process, and because the screws are inserted toward the lateral side, there is less soft tissue development than with the c...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12122
更新日期:2014-08-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of residual varus and valgus deformity on the stress distribution of the knee joint after tibial fracture malunion. METHODS:Fourteen adult cadaver specimens were selected to establish the models of tibial fractures, which were fixed subsequently at neutral position (anatomical reduc...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12668
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:To investigate the efficiency of anterior decompression on the proximal-type cervical spondylotic amyotrophy patients. METHODS:This was a retrospective analysis. From January 2014 to November 2017, 21 patients with proximal-type cervical spondylotic amyotrophy (CSA) underwent anterior decompression. There we...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12654
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:To present the use of an intermediate dorsal neurocutaneous flap for the reconstruction of defects on the distal foot. METHODS:From September 2016 to October 2018, five patients (mean age at operation 33.8 years; range, 7-70 years; female/male = 2/3) with skin defects on one of their feet caused by road-traf...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12623
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVE:To investigate the bone hardness of different anatomical regions of the human radius and its impact on the pullout strength of screws. METHODS:Fresh radius bones were obtained from three donated cadavers. They were divided into three parts: proximal metaphysis, shaft, and distal metaphysis. The proximal meta...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12436
更新日期:2019-04-01 00:00:00
abstract::The authors describe a hybrid technique that involves a combination of open decompression and posterior lumbar interbody fusion (PLIF) and percutaneously inserted pedicle screws. This technique allows performance of PLIF and decompression via a midline incision and approach without compromising operative time and visu...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12042
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVE:To study the clinical features and diagnosis of bursal-side partial-thickness rotator cuff tears. METHODS:From August 1999 to June 2006, 38 patients with bursal-side partial-thickness rotator cuff tear were evaluated. Twenty-eight men and ten women of average age 45.7 years (range, 18-69 years) with 11 left ...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2010.00097.x
更新日期:2010-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the bi-planar robot navigation system for insertion of cannulated screws in femoral neck fractures. METHOD:Between January 2016 and December 2016, 60 patients with femoral neck fractures were separately treated using percutaneous cannulated screws assisted by the bi-planar robot navigation system...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12450
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:The aim of the present study was to evaluate the effect of patellar denervation (PD) in preventing anterior knee pain (AKP) and improving knee function after total knee arthroplasty (TKA) without patellar resurfacing, and to help surgeons decide whether or not to use PD in TKA. METHODS:The electronic databas...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12815
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:To investigate the methods for, and clinical outcome of, the operative treatment of thoracolumbar fractures through an approach via the paravertebral muscle (PVM). METHODS:From June 2005 to August 2006, 62 patients, comprising 48 men and 14 women with an average age of 45.2 years (range, 21-58) with thoracol...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1757-7861.2009.00032.x
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVE:To describe a new method which involves anatomical distal clavicle plate fixation and titanium alloy cable system-augmented coracoclavicular ligament reconstruction to manage Neer type IIb distal clavicle fracture. METHODS:Between January 2013 and June 2018, 28 patients with acute Neer type IIb lateral clavi...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12893
更新日期:2021-01-22 00:00:00
abstract:OBJECTIVE:To compare the biomechanical properties of single- and two-segment fusion for Denis type B spinal fracture. METHODS:Two female patients with Denis type B L1 vertebral fractures were studied. Both patients had achieved intervertebral fusion by 1 year postoperatively, at which time CT data were collected, incl...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12068
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVE:To establish a three-dimensional finite element model of the L(5)/S(1) motion segment with percutaneous axial lumbosacral screw and analyze the biomechanical stress on the screw. METHODS:With the help of related software and the Mechanical Virtual Human of China, a three-dimensional finite element model was ...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2010.00088.x
更新日期:2010-08-01 00:00:00
abstract:OBJECTIVE:To investigate delay in diagnosis by both patients and doctors, and to evaluate its effect on outcomes of high-grade sarcoma of bone in a single-referral oncological center. METHODS:Fifty-four patients with osteosarcoma, 29 with Ewing sarcoma and 19 with chondrosarcoma were enrolled in this retrospective stu...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12239
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVE:To assess whether computed tomography (CT)-based 3-dimensional (3D) computerized pre-operative planning is accurate and reliable in patients with high-riding dislocation developmental dysplasia of the hip (DDH) undergoing total hip arthroplasty (THA). METHODS:Between September 2009 and February 2011, a prosp...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12099
更新日期:2014-05-01 00:00:00
abstract:OBJECTIVE:To evaluate the functional outcomes of arthroscopic anatomical repair of anterior talofibular ligament (ATFL) in the treatment of chronic lateral ankle instability (CLAI) during medium- and long-term follow-up. METHODS:From September 2014 to August 2017, the data of 37 patients (23 males, 14 females; 12 left...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12651
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVE:To introduce an iliosacral screw fixation guide and evaluate its efficacy in fixation of sacroiliac joint fracture-dislocations. METHODS:Between January 2011 and May 2011, eight patients (five men, three women) with sacroiliac joint fracture-dislocation underwent percutaneous iliosacral screw fixation with t...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/j.1757-7861.2011.00162.x
更新日期:2012-02-01 00:00:00
abstract::A 66-year-old woman had sustained crush injury 3 hours prior to her presentation to our hospital. The diagnosis was defined as lateral tibial plateau fracture of the right knee (Schatzker III). Supine position was set up and a pad was put under the affected hip. After sterilization of the surgical field the sterilized...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12205
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVES:A series of constant anatomical structures were used as guide targets for screw placement to improve the accuracy of cortical screw placement and reduce surgical injury and fluoroscopy radiation. The most commonly used angles and distances between the cortical bone screw insertion point and the lateral margi...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12775
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:Knee arthroscopy is a commonly performed orthopedic procedure. Post-operatively, adequate pain relief reduces the surgical stress response and patient's morbidity and facilitates rehabilitation. The analgesic effect of dexmedetomidine (2 μg/kg body weight) as an adjunct to ropivacaine in knee arthroscopic kne...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/os.12182
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVE:To examine the results of an anular closure device for prevention of lumbar disc reherniation in daily routine practice. METHODS:Fifty patients with large anular defects were treated with limited discectomy and a bone-anchored anular closure device. The device physically occludes the defect in the anulus fib...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12495
更新日期:2019-06-01 00:00:00
abstract::Diaphyseal defects in long bones of extremities following segmental resection are very common in the clinic, and reconstruction remains a great challenge. Although there are many treatments for diaphyseal defects at present, reconstruction with an intercalary endoprosthesis may be an optimal method. We demonstrate a s...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12391
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:To investigate the outcomes of total knee arthroplasty (TKA) in patients with a prior femoral or tibial fracture, and identify the risk factors for surgical site complications and reoperations. METHODS:Seventy-one TKAs performed in 71 patients with a prior tibial or femoral fracture between January 2005 and ...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12610
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVE:To describe the technique of primary repair of medial collateral ligament (MCL) insufficiency using a screw and rectangular spiked washer in a case series of 14 patients. METHODS:Fourteen patients undergoing MCL repair by a screw and rectangular spiked washer during TKA between March 2018 and March 2019 were...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12818
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:To evaluate the clinical outcomes of anterior and posterior instrumentation with different debridement and graft fusion methods for multi-level contiguous thoracic spinal tuberculosis. METHODS:We retrospectively evaluated 81 patients with multi-level contiguous thoracic spinal tuberculosis who underwent ante...
journal_title:Orthopaedic surgery
pub_type: 杂志文章
doi:10.1111/os.12288
更新日期:2016-11-01 00:00:00
abstract::Gouty arthritis is an extremely painful condition that causes functional impairment. Gouty arthritis has become increasingly complex because of multiple comorbidities, iatrogenic factors and hyperuricemia that is refractory to treatment. In this review, we present a general overview of gouty arthritis including its pa...
journal_title:Orthopaedic surgery
pub_type: 杂志文章,评审
doi:10.1111/os.12024
更新日期:2013-02-01 00:00:00