LncRNA-H19 Modulates Wnt/β-catenin Signaling by Targeting Dkk4 in Hindlimb Unloaded Rat.


OBJECTIVE:To investigate the biological functions of long noncoding RNA-H19 (H19) in the pathogenesis of disuse osteoporosis (DOP). METHODS:Fifty-four male Sprague Dawley (SD) rats were randomly divided into three groups: baseline control (BC, 6), age-matched control (AC, 24), and hindlimb unloading (HLU, 24). The rats in the BC group were sacrificed at the beginning of the experiment, while the AC and HLU rats were sacrificed at different times (7, 14, 21 and 28 days after HLU). The DOP model was verified by micro-CT scan, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to quantify the expression of osteogenic genes (OPG, RunX2 and OPG). Gene sequencing and bioinformatic analysis were performed to find H19 target genes and the associated signaling pathway, which were first verified on tissue samples. Further verification was performed by knocking down the H19 and related gene in rat osteoblast cell line (UMR106 cell). Then, the changes of associated signaling pathway and osteogenic function were examined to confirm the prediction of the bioinformatic analysis. RESULTS:Micro-CT scans and quantitative real-time polymerase chain reaction (qRT-PCR) tests showed progressively deteriorated trabecular bone and decreased level of osteogenic genes in the metaphysis of distal femur during HLU, indicating the successful establishment of a DOP model. According to RNA sequencing, 1351 mRNA and 464 lncRNA were abnormally expressed in response to mechanical unloading, in which the H19 decreased 2.86 fold in HLU rats. There were 1426 mRNA predicted to be the target genes of H19, and KEGG pathway analysis suggested that Wnt signaling pathway (Wnt signaling) was the top pathway responsible for these target genes. In the Wnt-associated genes targeted by H19, 11 were differentially expressed between HLU and AC rats, among which Dkk4 increased 2.44 fold in HLU rats when compared to normal controls. These results of sequencing and bioinformatic analysis were confirmed by the low expression of H19, overexpression of Dkk4 and inhibited Wnt signaling observed in DOP rats. Subsequent in vitro cell assay further demonstrated that knockdown of H19 led to upregulation of Dkk4, and inhibition of Wnt signaling and osteogenic function in UMR106 cell. These effects can be greatly reversed after application of knocking down Dkk4. CONCLUSION:Our findings demonstrated that low expression of H19, induced by mechanical unloading, leads to development of DOP through inhibition of Wnt signaling by promoting Dkk4 expression.


Orthop Surg


Orthopaedic surgery


Li B,Liu J,Zhao J,Ma JX,Jia HB,Zhang Y,Xing GS,Ma XL




Has Abstract


2017-08-01 00:00:00












  • Guideline for Posterior Atlantoaxial Internal Fixation Assisted by Orthopaedic Surgical Robot.

    abstract::Atlantoaxial transarticular facet screw fixation (Magerl technique) and C1 lateral mass screws combined with C2 pedicle screws fixation (Harms technique) are the most commonly used techniques for posterior internal fixation in the upper cervical spine. Upper cervical spinal surgery is a technically demanding and chall...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章,实务指引


    authors: Tian W,Liu YJ,Liu B,He D,Wu JY,Han XG,Zhao JW,Fan MX,Technical Committee on Medical Robot Engineering of Chinese Society of Biomedical Engineering.,Technical Consulting Committee of National Robotic Orthopaedic Surgery Application Cent

    更新日期:2019-04-01 00:00:00

  • Biomechanical Study on the Stress Distribution of the Knee Joint After Tibial Fracture Malunion with Residual Varus-Valgus Deformity.

    abstract:OBJECTIVE:To investigate the effect of residual varus and valgus deformity on the stress distribution of the knee joint after tibial fracture malunion. METHODS:Fourteen adult cadaver specimens were selected to establish the models of tibial fractures, which were fixed subsequently at neutral position (anatomical reduc...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Li M,Chang H,Wei N,Chang W,Yan Y,Jin Z,Chen W

    更新日期:2020-06-01 00:00:00

  • Intra-articular Adjuvant Analgesics following Knee Arthroscopy: Comparison between Single and Double Dose Dexmedetomidine and Ropivacaine A Multicenter Prospective Double-blind Trial.

    abstract:OBJECTIVE:Knee arthroscopy is a commonly performed orthopedic procedure. Post-operatively, adequate pain relief reduces the surgical stress response and patient's morbidity and facilitates rehabilitation. The analgesic effect of dexmedetomidine (2 μg/kg body weight) as an adjunct to ropivacaine in knee arthroscopic kne...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Panigrahi R,Roy R,Mahapatra AK,Prasad A,Priyadarshi A,Palo N

    更新日期:2015-08-01 00:00:00

  • Surgical treatment of open pilon fractures.

    abstract:OBJECTIVE:To discuss the methods, timing and clinical outcomes of surgical treatment for open pilon fractures. METHODS:From April 2003 to July 2008, 28 patients with open pilon fractures were treated. All had type C fractures according to the Arbeitsgemeinschaft für osteosynthesefragen-Association for the Study of Int...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Zeng XT,Pang GG,Ma BT,Mei XL,Sun X,Wang J,Jia P

    更新日期:2011-02-01 00:00:00

  • A New Intraoperative Syndesmosis Instability Classification System: Utility and Medium-term Results in Closed Displaced Ankle Fractures.

    abstract:OBJECTIVE:To investigate the utility and medium-term results of a new intra-operative classification system for distal tibiofibular syndesmosis injury in ankle fractures. METHODS:Between January 2010 and January 2015, 116 patients diagnosed with displaced closed Weber B and C ankle fractures were treated in our depart...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Qiu HB,Jiang J,Porter D

    更新日期:2017-11-01 00:00:00

  • Delay in Diagnosis and Its Effect on Clinical Outcome in High-grade Sarcoma of Bone: A Referral Oncological Centre Study.

    abstract:OBJECTIVE:To investigate delay in diagnosis by both patients and doctors, and to evaluate its effect on outcomes of high-grade sarcoma of bone in a single-referral oncological center. METHODS:Fifty-four patients with osteosarcoma, 29 with Ewing sarcoma and 19 with chondrosarcoma were enrolled in this retrospective stu...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Goedhart LM,Gerbers JG,Ploegmakers JJ,Jutte PC

    更新日期:2016-05-01 00:00:00

  • F-Shaped Osteotomy Combined with Basal Opening Wedge Osteotomy for Severe Hallux Valgus.

    abstract:OBJECTIVE:To evaluate the safety and effectiveness of osteotomy adjacent to the articular surface of the metatarsal head combined with basal opening wedge osteotomy for severe hallux valgus. METHODS:The double osteotomy procedure was carried out in 56 patients (72 feet) with severe hallux valgus deformity, with an ave...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Li C,Lu L,Zhang Y,Ai-Xin-Jue-Luo QC,Wang ZT,Wang JF

    更新日期:2019-08-01 00:00:00

  • Observational Study of Total Knee Arthroplasty in Aseptic Revision Surgery: Clinical Results.

    abstract:OBJECTIVE:To review the long-term clinical results after revision surgery and the relationship between the different clinical variables involved with a failed total knee arthroplasty (TKA) and its evolution to provide a better understanding of the current treatment methods. METHODS:The present study involved 89 subjec...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Oliver G,Jaldin L,Camprubí E,Cortés G

    更新日期:2020-02-01 00:00:00

  • Finite element analysis of the screw in percutaneous axial lumbosacral interbody fusion.

    abstract:OBJECTIVE:To establish a three-dimensional finite element model of the L(5)/S(1) motion segment with percutaneous axial lumbosacral screw and analyze the biomechanical stress on the screw. METHODS:With the help of related software and the Mechanical Virtual Human of China, a three-dimensional finite element model was ...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Xu HG,Yang XM,Wu TL,Wang H,Chen XW,Wang LT,Jin S,Liu P

    更新日期:2010-08-01 00:00:00

  • Comparison of Superior-Level Facet Joint Violations Between Robot-Assisted Percutaneous Pedicle Screw Placement and Conventional Open Fluoroscopic-Guided Pedicle Screw Placement.

    abstract:OBJECTIVE:To compare the superior-level facet joint violations (FJV) between robot-assisted (RA) percutaneous pedicle screw placement and conventional open fluoroscopic-guided (FG) pedicle screw placement in a prospective cohort study. METHODS:This was a prospective cohort study without randomization. One-hundred pati...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Zhang Q,Xu YF,Tian W,Le XF,Liu B,Liu YJ,He D,Sun YQ,Yuan Q,Lang Z,Han XG

    更新日期:2019-10-01 00:00:00

  • Factors influencing segmental lumbar lordosis after lateral transpsoas interbody fusion.

    abstract:OBJECTIVE:Although contributions to sagittal alignment have been characterized for anterior, posterior and transforaminal lumbar interbody fusion, sagittal alignment after lateral transpsoas interbody fusion (LTIF) has not yet been characterized. This study examined the ability of LTIF to restore lumbar lordosis and id...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Kepler CK,Huang RC,Sharma AK,Meredith DS,Metitiri O,Sama AA,Girardi FP,Cammisa FP

    更新日期:2012-05-01 00:00:00

  • One-stage anterior release and reduction with posterior fusion for treatment of irreducible atlantoaxial dislocation.

    abstract:OBJECTIVE:To analyze the approach and feasibility of one-stage anterior release and reduction with posterior fusion for irreducible atlantoaxial dislocation. METHODS:Ten male and 6 female patients, with an average age of 36 years, including 13 patients with old trauma, 2 with rheumatoid disease, and 1 with os odontoid...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Hao DJ,He BR,Wu QN

    更新日期:2009-11-01 00:00:00

  • Hybrid technique for posterior lumbar interbody fusion: a combination of open decompression and percutaneous pedicle screw fixation.

    abstract::The authors describe a hybrid technique that involves a combination of open decompression and posterior lumbar interbody fusion (PLIF) and percutaneously inserted pedicle screws. This technique allows performance of PLIF and decompression via a midline incision and approach without compromising operative time and visu...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Mobbs RJ,Sivabalan P,Li J,Wilson P,Rao PJ

    更新日期:2013-05-01 00:00:00

  • Clinical Evaluation and Gait Characteristics before and after Total Knee Arthroplasty Based on a Portable Gait Analyzer.

    abstract:OBJECTIVE:To evaluate the effects of surgery and rehabilitation on patients undergoing total knee arthroplasty (TKA). METHODS:Twelve patients and 12 healthy controls were enrolled and their clinical scores evaluated by a doctor. Gait data, including walking velocity, stride length, single support time, foot fall and s...

    journal_title:Orthopaedic surgery

    pub_type: 临床试验,杂志文章


    authors: Zhang HH,Yan SH,Fang C,Guo XY,Zhang K

    更新日期:2016-08-01 00:00:00

  • Higher concentration of serum C-terminal cross-linking telopeptide of type I collagen is positively related with inflammatory factors in postmenopausal women with H-type hypertension and osteoporosis.

    abstract:OBJECTIVE:To investigate the changes of inflammatory factors and bone metabolism markers in postmenopausal women with H-type hypertension and to assess the relationship between them. METHODS:Postmenopausal women who were diagnosed with osteoporosis were selected as observation objects. Participants were divided into t...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Chen YN,Wei P,Yu Bs J

    更新日期:2019-12-01 00:00:00

  • Minimally Invasive Transforaminal Lumbar Interbody Fusion and Unilateral Fixation for Degenerative Lumbar Disease.

    abstract:OBJECTIVE:To evaluate the clinical effect of the minimally invasive transforaminal lumbar interbody fusion combined with posterolateral fusion and unilateral fixation using a tubular retractor in the management of degenerative lumbar disease. METHODS:A retrospective analysis was conducted to analyze the clinical outco...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Wang HW,Hu YC,Wu ZY,Wu HR,Wu CF,Zhang LS,Xu WK,Fan HL,Cai JS,Ma JQ

    更新日期:2017-08-01 00:00:00

  • Current concepts in the treatment of gouty arthritis.

    abstract::Gouty arthritis is an extremely painful condition that causes functional impairment. Gouty arthritis has become increasingly complex because of multiple comorbidities, iatrogenic factors and hyperuricemia that is refractory to treatment. In this review, we present a general overview of gouty arthritis including its pa...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章,评审


    authors: Fang ZH,Waizy H

    更新日期:2013-02-01 00:00:00

  • Functions of Exogenous RUNX2 in Giant Cell Tumor of Bone In Vitro.

    abstract:OBJECTIVES:This research aimed to investigate the relative level of Runt-related transcription factor 2 (RUNX2) in giant cell tumor of bone (GCTB). Through the histopathological similarities between osteoporosis and GCTB, the biological functions of exogenous RUNXS were demonstrated in GCTB cell lines. This generated a...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Jin YH,Zhang J,Zhu H,Fan GT,Zhou GX

    更新日期:2020-04-01 00:00:00

  • Corrections of lower limb deformities in patients with diastrophic dysplasia.

    abstract:OBJECTIVE:Accurate understanding of the cause of the underlying pathology in children with diastrophic dysplasia would help in designing targeted management of their locomotion. METHODS:Diastrophic dysplasia was diagnosed in twelve patients (nine girls and three boys; age range 1-14 years), all of whom presented with ...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Al Kaissi A,Kenis V,Melchenko E,Chehida FB,Ganger R,Klaushofer K,Grill F

    更新日期:2014-11-01 00:00:00

  • Diagnosis of bursal-side partial-thickness rotator cuff tears.

    abstract:OBJECTIVE:To study the clinical features and diagnosis of bursal-side partial-thickness rotator cuff tears. METHODS:From August 1999 to June 2006, 38 patients with bursal-side partial-thickness rotator cuff tear were evaluated. Twenty-eight men and ten women of average age 45.7 years (range, 18-69 years) with 11 left ...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Xiao J,Cui GQ,Wang JQ

    更新日期:2010-11-01 00:00:00

  • Anatomy and Imaging Studies on Cortical Bone Screw Freehand Placement Applying Anatomical Targeting Technology.

    abstract:OBJECTIVES:A series of constant anatomical structures were used as guide targets for screw placement to improve the accuracy of cortical screw placement and reduce surgical injury and fluoroscopy radiation. The most commonly used angles and distances between the cortical bone screw insertion point and the lateral margi...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Rexiti P,Aierken A,Sadeer A,Wang S,Abuduwali N,Deng Q,Sheng WB,Guo HL

    更新日期:2020-12-01 00:00:00

  • Isthmus-guided cortical bone trajectory for pedicle screw insertion.

    abstract::Herein is described cortical bone trajectory (CBT), a new path for pedicle screw insertion for lumbar vertebral fusion. Because the points of insertion are under the end of the inferior articular process, and because the screws are inserted toward the lateral side, there is less soft tissue development than with the c...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Iwatsuki K,Yoshimine T,Ohnishi Y,Ninomiya K,Ohkawa T

    更新日期:2014-08-01 00:00:00

  • Surgical treatment of thoracolumbar fracture through an approach via the paravertebral muscle.

    abstract:OBJECTIVE:To investigate the methods for, and clinical outcome of, the operative treatment of thoracolumbar fractures through an approach via the paravertebral muscle (PVM). METHODS:From June 2005 to August 2006, 62 patients, comprising 48 men and 14 women with an average age of 45.2 years (range, 21-58) with thoracol...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章,随机对照试验


    authors: Pang W,Zhang GL,Tian W,Sun D,Li N,Yuan Q,Zhang B,Wang YQ,Liu W

    更新日期:2009-08-01 00:00:00

  • Spinal Metastatic Disease: A Review of the Role of the Multidisciplinary Team.

    abstract::Historically, a simple approach centered on palliation was applicable to the majority of patients with metastatic spinal disease. With advances in diagnosis and treatment, a more complicated algorithm has devolved requiring a multidisciplinary approach with institutional commitment and support. We performed a database...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章,评审


    authors: Curtin M,Piggott RP,Murphy EP,Munigangaiah S,Baker JF,McCabe JP,Devitt A

    更新日期:2017-05-01 00:00:00

  • Loss of Reduction after Cephalomedullary Nail Fixation of Intertrochanteric Femoral Fracture: A Brief Report.

    abstract:OBJECTIVE:At present, cephalomedullary nail is the most frequently used implant in the management of intertrochanteric fractures around the world. The implant design and fixation techniques of the cephalomedullary nail have been continuously improved to ensure uncomplicated bone union during the past decade. However, a...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Pang Y,He QF,Zhu LL,Bian ZY,Li MQ

    更新日期:2020-12-01 00:00:00

  • Effects of Unilateral Tourniquet Used in Patients Undergoing Simultaneous Bilateral Total Knee Arthroplasty.

    abstract:OBJECTIVE:To assess the benefits of use of a tourniquet in one limb in patients undergoing simultaneous bilateral total knee arthroplasty (TKA). METHODS:A prospective randomized trial was designed to evaluate the outcomes of unilateral tourniquet use during simultaneous bilateral TKA. A total of 52 (36 women and 16 me...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章,随机对照试验


    authors: Liu PL,Li DQ,Zhang YK,Lu QS,Ma L,Bao XZ,Zhang M

    更新日期:2017-05-01 00:00:00

  • Novel Screw Insertion Method for Anterior Surgical Treatment of Unstable Thoracolumbar Fracture: Quadrant Positioning Method.

    abstract:OBJECTIVE:To develop a novel screw positioning method to improve the treatment of unstable thoracolumbar fractures. METHODS:A total of 72 patients with unstable thoracolumbar fractures who were treated with anterior screw-rod interfixation from January 2011 to October 2015 were included in this clinical study. Those p...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Wang S,Duan CY,Yang H,Kang JP,Wang Q

    更新日期:2019-08-01 00:00:00

  • Efficacy and Safety of Tranexamic Acid in Intertrochanteric Fractures: A Single-Blind Randomized Controlled Trial.

    abstract:OBJECTIVE:To investigate the efficacy and safety of tranexamic acid (TXA) in the reduction of bleeding and the need for transfusion in elderly intertrochanteric fracture patients. METHODS:A total of 100 patients with intertrochanteric fractures undergoing surgery were enrolled and randomly allocated to the TXA group i...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章,随机对照试验


    authors: Zhou XD,Zhang Y,Jiang LF,Zhang JJ,Zhou D,Wu LD,Huang Y,Xu NW

    更新日期:2019-08-01 00:00:00

  • Comparison of Different Insertion Techniques for Lumbosacral Fixation Improvement: A Finite Element Study.

    abstract:OBJECTIVE:We create a new S1 cortical screw trajectory technique using 3D reconstruction and the finite element (FE) method to provide a more reliable theoretical basis for clinical practices and to advance internal fixation technology for treatment of lumbosacral degenerative diseases. METHODS:This retrospective stud...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Han DP,Wang JY

    更新日期:2020-02-01 00:00:00

  • Synergistic Effects of Kartogenin and Transforming Growth Factor-β3 on Chondrogenesis of Human Umbilical Cord Mesenchymal Stem Cells In Vitro.

    abstract:OBJECTIVE:To explore the effect of kartogenin (KGN) on proliferation and chondrogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSC) in vitro, and the synergistic effects of KGN and transforming growth factor (TGF)-β3 on hUCMSC. METHODS:Human umbilical cord mesenchymal stem cells were isolated...

    journal_title:Orthopaedic surgery

    pub_type: 杂志文章


    authors: Zhao Y,Teng B,Sun X,Dong Y,Wang S,Hu Y,Wang Z,Ma X,Yang Q

    更新日期:2020-06-01 00:00:00