Modulation of Monocyte-Driven Myositis in Alphavirus Infection Reveals a Role for CX3CR1+ Macrophages in Tissue Repair.

abstract：:A method for use in high-throughput screening of bacteria for the production of long-chain hydrocarbons and ketones by monitoring fluorescent light emission in the presence of Nile red is described. Nile red has previously been used to screen for polyhydroxybutyrate (PHB) and fatty acid esters, but this is the first r...

journal_title：mBio

authors： Pinzon NM,Aukema KG,Gralnick JA,Wackett LP

更新日期：2011-06-28 00:00:00

abstract：:We showed previously that the histone lysine methyltransferase (HKMT) H3K27me3 (EZH2) is the catalytic subunit of Polycomb repressive complex 2 (PRC2) and is required for the maintenance of HIV-1 latency in Jurkat T cells. Here we show, by using chromatin immunoprecipitation experiments, that both PRC2 and euchromatic...

journal_title：mBio

authors： Nguyen K,Das B,Dobrowolski C,Karn J

更新日期：2017-02-28 00:00:00

abstract：UNLABELLED:Exploiting mechanisms of utilizing the sugar d-galactose in Escherichia coli as a model system, we explored the consequences of accumulation of critical intermediates of the d-galactose metabolic pathways by monitoring cell growth, metabolites, and transcript profiles. These studies revealed both metabolic n...

journal_title：mBio

authors： Lee SJ,Trostel A,Adhya S

更新日期：2014-01-28 00:00:00

abstract：:Once HIV-1 enters a cell, the viral core is uncoated by a poorly understood mechanism and the HIV-1 genomic RNA is reverse transcribed into DNA. Host cell factors are essential for these processes, although very few reverse transcription complex binding host cell factors have been convincingly shown to affect uncoatin...

journal_title：mBio

authors： Rawle DJ,Li D,Swedberg JE,Wang L,Soares DC,Harrich D

更新日期：2018-03-27 00:00:00

abstract：UNLABELLED:Susceptibility to mouse adenovirus type 1 (MAV-1) is mouse strain dependent; susceptible mice die from hemorrhagic encephalomyelitis. The MAV-1 susceptibility quantitative trait locus Msq1 accounts for ~40% of the phenotypic (brain viral load) variance that occurs between resistant BALB/c and susceptible SJL...

journal_title：mBio

authors： Hsu TH,Althaus IW,Foreman O,Spindler KR

更新日期：2012-05-29 00:00:00

abstract：UNLABELLED:Cholera continues to be a global threat, with high rates of morbidity and mortality. In 2011, a cholera outbreak occurred in Palawan, Philippines, affecting more than 500 people, and 20 individuals died. Vibrio cholerae O1 was confirmed as the etiological agent. Source attribution is critical in cholera outb...

journal_title：mBio

authors： Klinzing DC,Choi SY,Hasan NA,Matias RR,Tayag E,Geronimo J,Skowronski E,Rashed SM,Kawashima K,Rosenzweig CN,Gibbons HS,Torres BC,Liles V,Alfon AC,Juan ML,Natividad FF,Cebula TA,Colwell RR

更新日期：2015-04-21 00:00:00

abstract：:Cellular sensing of bacterial RNA is increasingly recognized as a determinant of host-pathogen interactions. The intracellular pathogen Listeria monocytogenes induces high levels of type I interferons (alpha/beta interferons [IFN-α/β]) to create a growth-permissive microenvironment during infection. We previously demo...

journal_title：mBio

authors： Frantz R,Teubner L,Schultze T,La Pietra L,Müller C,Gwozdzinski K,Pillich H,Hain T,Weber-Gerlach M,Panagiotidis GD,Mostafa A,Weber F,Rohde M,Pleschka S,Chakraborty T,Abu Mraheil M

更新日期：2019-10-08 00:00:00

abstract：:We have previously identified Vibrio cholerae mutants in which the stress response to subinhibitory concentrations of aminoglycoside is altered. One gene identified, VC1636, encodes a putative DNA/RNA helicase, recently named RadD in Escherichia coli Here we combined extensive genetic characterization and high-through...

journal_title：mBio

authors： Negro V,Krin E,Aguilar Pierlé S,Chaze T,Giai Gianetto Q,Kennedy SP,Matondo M,Mazel D,Baharoglu Z

更新日期：2019-07-02 00:00:00

abstract：:Bacillus anthracis-a Gram-positive, spore-forming bacterium-causes anthrax, a highly lethal disease with high bacteremia titers. Such rapid growth requires ample access to nutrients, including iron. However, access to this critical metal is heavily restricted in mammals, which requires B. anthracis to employ petrobact...

journal_title：mBio

authors： Hagan AK,Tripathi A,Berger D,Sherman DH,Hanna PC

更新日期：2017-09-12 00:00:00

abstract：UNLABELLED:We studied the flocculation mechanism at the molecular level by determining the atomic structures of N-Flo1p and N-Lg-Flo1p in complex with their ligands. We show that they have similar ligand binding mechanisms but distinct carbohydrate specificities and affinities, which are determined by the compactness o...

journal_title：mBio

authors： Goossens KV,Ielasi FS,Nookaew I,Stals I,Alonso-Sarduy L,Daenen L,Van Mulders SE,Stassen C,van Eijsden RG,Siewers V,Delvaux FR,Kasas S,Nielsen J,Devreese B,Willaert RG

更新日期：2015-04-14 00:00:00

abstract：UNLABELLED:A mechanistic understanding of the determination and maintenance of the simplest bacterial cell shape, a sphere, remains elusive compared with that of more complex shapes. Cocci seem to lack a dedicated elongation machinery, and a spherical shape has been considered an evolutionary dead-end morphology, as a ...

journal_title：mBio

authors： Pereira AR,Hsin J,Król E,Tavares AC,Flores P,Hoiczyk E,Ng N,Dajkovic A,Brun YV,VanNieuwenhze MS,Roemer T,Carballido-Lopez R,Scheffers DJ,Huang KC,Pinho MG

更新日期：2016-09-06 00:00:00

abstract：UNLABELLED:A change in viral hemagglutinin (HA) receptor binding specificity from α2,3- to α2,6-linked sialic acid is necessary for highly pathogenic avian influenza (AI) virus subtype H5N1 to become pandemic. However, details of the human-adaptive change in the H5N1 virus remain unknown. Our database search of H5N1 cl...

journal_title：mBio

authors： Watanabe Y,Arai Y,Daidoji T,Kawashita N,Ibrahim MS,El-Gendy Eel-D,Hiramatsu H,Kubota-Koketsu R,Takagi T,Murata T,Takahashi K,Okuno Y,Nakaya T,Suzuki Y,Ikuta K

更新日期：2015-04-07 00:00:00

abstract：:The clonal population structure of Candida albicans suggests that (para)sexual recombination does not play an important role in the lifestyle of this opportunistic fungal pathogen, an assumption that is strengthened by the fact that most C. albicans strains are heterozygous at the mating type locus (MTL) and therefore...

journal_title：mBio

authors： Popp C,Ramírez-Zavala B,Schwanfelder S,Krüger I,Morschhäuser J

更新日期：2019-02-05 00:00:00

abstract：:Butanol production by Clostridium acetobutylicum is accompanied by coproduction of acetone and ethanol, which reduces the yield of butanol and increases the production cost. Here, we report development of several clostridial aldehyde/alcohol dehydrogenase (AAD) variants showing increased butanol selectivity by a serie...

journal_title：mBio

authors： Cho C,Hong S,Moon HG,Jang YS,Kim D,Lee SY

更新日期：2019-01-22 00:00:00

abstract：:In contrast to usual laboratory conditions, most bacteria in the human body grow in biofilms. Encased in a structured matrix, many pathogens display heightened resistance to antibiotics. Pseudomonas aeruginosa lung infections in cystic fibrosis patients represent a prime example of the clinical challenges that antibio...

journal_title：mBio

authors： Brüssow H

更新日期：2012-04-03 00:00:00

abstract：:During DNA replication, stalling can occur when the replicative DNA polymerases encounter lesions or hard-to replicate regions. Under these circumstances, the processivity factor PCNA gets ubiquitylated at lysine 164, inducing the DNA damage tolerance (DDT) mechanisms that can bypass lesions encountered during DNA rep...

journal_title：mBio

authors： Arbel M,Bronstein A,Sau S,Liefshitz B,Kupiec M

更新日期：2020-05-05 00:00:00

abstract：UNLABELLED:Members of the genus Vibrio include many pathogens of humans and marine animals that share genetic information via horizontal gene transfer. Hence, the Vibrio pan-genome carries the potential to establish new pathogenic strains by sharing virulence determinants, many of which have yet to be characterized. He...

journal_title：mBio

authors： Ray A,Kinch LN,de Souza Santos M,Grishin NV,Orth K,Salomon D

更新日期：2016-07-26 00:00:00

abstract：:Ebola virus (EBOV) protein VP35 inhibits production of interferon alpha/beta (IFN) by blocking RIG-I-like receptor signaling pathways, thereby promoting virus replication and pathogenesis. A high-throughput screening assay, developed to identify compounds that either inhibit or bypass VP35 IFN-antagonist function, ide...

journal_title：mBio

authors： Luthra P,Aguirre S,Yen BC,Pietzsch CA,Sanchez-Aparicio MT,Tigabu B,Morlock LK,García-Sastre A,Leung DW,Williams NS,Fernandez-Sesma A,Bukreyev A,Basler CF

更新日期：2017-04-04 00:00:00

abstract：:Mannitol-1-phosphate dehydrogenase (M1PDH) is a key enzyme in Staphylococcus aureus mannitol metabolism, but its roles in pathophysiological settings have not been established. We performed comprehensive structure-function analysis of M1PDH from S. aureus USA300, a strain of community-associated methicillin-resistant ...

journal_title：mBio

authors： Nguyen T,Kim T,Ta HM,Yeo WS,Choi J,Mizar P,Lee SS,Bae T,Chaurasia AK,Kim KK

更新日期：2019-07-09 00:00:00

abstract：:Uncultured and therefore uncharacterized Bacteroidetes lineages are ubiquitous in many natural ecosystems which specialize in lignocellulose degradation. However, their metabolic contribution remains mysterious, as well-studied cultured Bacteroidetes have been shown to degrade only soluble polysaccharides within the h...

journal_title：mBio

authors： Naas AE,Mackenzie AK,Mravec J,Schückel J,Willats WG,Eijsink VG,Pope PB

更新日期：2014-08-05 00:00:00

abstract：:The facultative intracellular pathogen Listeria monocytogenes, like many related Firmicutes, uses the nucleotide second messenger cyclic di-AMP (c-di-AMP) to adapt to changes in nutrient availability, osmotic stress, and the presence of cell wall-acting antibiotics. In rich medium, c-di-AMP is essential; however, muta...

journal_title：mBio

authors： Peterson BN,Young MKM,Luo S,Wang J,Whiteley AT,Woodward JJ,Tong L,Wang JD,Portnoy DA

更新日期：2020-08-25 00:00:00

abstract：:Gammaherpesviruses (γHVs) are generally considered host specific and to have codiverged with their hosts over millions of years. This tenet is challenged here by broad-scale phylogenetic analysis of two viral genes using the largest sample of mammalian γHVs to date, integrating for the first time bat γHV sequences ava...

journal_title：mBio

authors： Escalera-Zamudio M,Rojas-Anaya E,Kolokotronis SO,Taboada B,Loza-Rubio E,Méndez-Ojeda ML,Arias CF,Osterrieder N,Greenwood AD

更新日期：2016-11-08 00:00:00

abstract：:The biosynthesis of antioxidant pigments, namely, betalains, was believed to be restricted to Caryophyllales plants. This paper changes this paradigm, and enzyme mining from bacterial hosts promoted the discovery of bacterial cultures producing betalains. The spectrum of possible sources of betalain pigments in nature...

journal_title：mBio

authors： Contreras-Llano LE,Guerrero-Rubio MA,Lozada-Ramírez JD,García-Carmona F,Gandía-Herrero F

更新日期：2019-03-19 00:00:00

abstract：:Simian virus 40 (SV40) is a nonenveloped DNA virus that traffics through the endoplasmic reticulum (ER) en route to the nucleus, but the mechanisms of capsid disassembly and ER exit are poorly understood. We conducted an unbiased RNA interference screen to identify cellular genes required for SV40 infection. SV40 infe...

journal_title：mBio

authors： Goodwin EC,Lipovsky A,Inoue T,Magaldi TG,Edwards AP,Van Goor KE,Paton AW,Paton JC,Atwood WJ,Tsai B,DiMaio D

更新日期：2011-06-14 00:00:00

abstract：:Plasmodium vivax causes heavy burdens of disease across malarious regions worldwide. Mature P. vivax asexual and transmissive gametocyte stages occur in the blood circulation, and it is often assumed that accumulation/sequestration in tissues is not an important phase in their development. Here, we present a systemati...

journal_title：mBio

authors： Obaldia N 3rd,Meibalan E,Sa JM,Ma S,Clark MA,Mejia P,Moraes Barros RR,Otero W,Ferreira MU,Mitchell JR,Milner DA,Huttenhower C,Wirth DF,Duraisingh MT,Wellems TE,Marti M

更新日期：2018-05-08 00:00:00

abstract：:Food-borne pathogens are ongoing problems, and new pathogens are emerging. The impact of fungi, however, is largely underestimated. Recently, commercial yogurts contaminated with Mucor circinelloides were sold, and >200 consumers became ill with nausea, vomiting, and diarrhea. Mucoralean fungi cause the fatal fungal i...

journal_title：mBio

authors： Lee SC,Billmyre RB,Li A,Carson S,Sykes SM,Huh EY,Mieczkowski P,Ko DC,Cuomo CA,Heitman J

更新日期：2014-07-08 00:00:00

abstract：:Malaria parasites have a complex life cycle with multiple developmental stages in mosquito and vertebrate hosts, and different developmental stages express unique sets of genes. Unexpectedly, many transcription factors (TFs) commonly found in eukaryotic organisms are absent in malaria parasites; instead, a family of g...

journal_title：mBio

authors： Zhang C,Li Z,Cui H,Jiang Y,Yang Z,Wang X,Gao H,Liu C,Zhang S,Su XZ,Yuan J

更新日期：2017-12-12 00:00:00

abstract：UNLABELLED:Mycobacterium tuberculosis depends on aerobic respiration for growth and utilizes an aa3-type cytochrome c oxidase for terminal electron transfer. Cytochrome c maturation in bacteria requires covalent attachment of heme to apocytochrome c, which occurs outside the cytoplasmic membrane. We demonstrate that in...

journal_title：mBio

authors： Small JL,Park SW,Kana BD,Ioerger TR,Sacchettini JC,Ehrt S

更新日期：2013-09-17 00:00:00

abstract：:Staphylococcus aureus is an important human pathogen whose success is largely attributed to its vast arsenal of virulence factors that facilitate its invasion into, and survival within, the human host. The expression of these virulence factors is controlled by the quorum sensing accessory gene regulator (Agr) system. ...

journal_title：mBio

authors： Gor V,Takemura AJ,Nishitani M,Higashide M,Medrano Romero V,Ohniwa RL,Morikawa K

更新日期：2019-08-06 00:00:00

abstract：:Streptococcus pneumoniae, a major cause of pneumonia, sepsis, and meningitis worldwide, has the nasopharynges of small children as its main ecological niche. Depletion of pneumococci from this niche would reduce the disease burden and could be achieved using small molecules with narrow-spectrum antibacterial activity....

journal_title：mBio

authors： Reithuber E,Nannapaneni P,Rzhepishevska O,Lindgren AEG,Ilchenko O,Normark S,Almqvist F,Henriques-Normark B,Mellroth P

更新日期：2020-12-15 00:00:00