Abstract:
UNLABELLED:Members of the genus Vibrio include many pathogens of humans and marine animals that share genetic information via horizontal gene transfer. Hence, the Vibrio pan-genome carries the potential to establish new pathogenic strains by sharing virulence determinants, many of which have yet to be characterized. Here, we investigated the virulence properties of Vibrio proteolyticus, a Gram-negative marine bacterium previously identified as part of the Vibrio consortium isolated from diseased corals. We found that V. proteolyticus causes actin cytoskeleton rearrangements followed by cell lysis in HeLa cells in a contact-independent manner. In search of the responsible virulence factor involved, we determined the V. proteolyticus secretome. This proteomics approach revealed various putative virulence factors, including active type VI secretion systems and effectors with virulence toxin domains; however, these type VI secretion systems were not responsible for the observed cytotoxic effects. Further examination of the V. proteolyticus secretome led us to hypothesize and subsequently demonstrate that a secreted hemolysin, belonging to a previously uncharacterized clan of the leukocidin superfamily, was the toxin responsible for the V. proteolyticus-mediated cytotoxicity in both HeLa cells and macrophages. Clearly, there remains an armory of yet-to-be-discovered virulence factors in the Vibrio pan-genome that will undoubtedly provide a wealth of knowledge on how a pathogen can manipulate host cells. IMPORTANCE:The pan-genome of the genus Vibrio is a potential reservoir of unidentified toxins that can provide insight into how members of this genus have successfully risen as emerging pathogens worldwide. We focused on Vibrio proteolyticus, a marine bacterium that was previously implicated in virulence toward marine animals, and characterized its interaction with eukaryotic cells. We found that this bacterium causes actin cytoskeleton rearrangements and leads to cell death. Using a proteomics approach, we identified a previously unstudied member of the leukocidin family of pore-forming toxins as the virulence factor responsible for the observed cytotoxicity in eukaryotic cells, as well as a plethora of additional putative virulence factors secreted by this bacterium. Our findings reveal a functional new clan of the leukocidin toxin superfamily and establish this pathogen as a reservoir of potential toxins that can be used for biomedical applications.
journal_name
mBiojournal_title
mBioauthors
Ray A,Kinch LN,de Souza Santos M,Grishin NV,Orth K,Salomon Ddoi
10.1128/mBio.01077-16subject
Has Abstractpub_date
2016-07-26 00:00:00issue
4issn
2150-7511pii
mBio.01077-16journal_volume
7pub_type
杂志文章相关文献
mBio文献大全abstract:UNLABELLED:Male circumcision reduces female-to-male HIV transmission. Hypothesized mechanisms for this protective effect include decreased HIV target cell recruitment and activation due to changes in the penis microbiome. We compared the coronal sulcus microbiota of men from a group of uncircumcised controls (n = 77) a...
journal_title:mBio
pub_type: 杂志文章,随机对照试验
doi:10.1128/mBio.00076-13
更新日期:2013-04-16 00:00:00
abstract::Peroxisomes are found in essentially all eukaryotic cells and have been described as important hubs in innate sensing and the induction of type III interferons upon viral infection. Nevertheless, it remains poorly investigated how viral pathogens modulate biogenesis or function of peroxisomes to evade innate sensing a...
journal_title:mBio
pub_type: 评论,杂志文章
doi:10.1128/mBio.00967-20
更新日期:2020-05-26 00:00:00
abstract::Host cytokine responses to Brucella abortus infection are elicited predominantly by the deployment of a type IV secretion system (T4SS). However, the mechanism by which the T4SS elicits inflammation remains unknown. Here we show that translocation of the T4SS substrate VceC into host cells induces proinflammatory resp...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00418-12
更新日期:2013-02-19 00:00:00
abstract:UNLABELLED:The genus Cyanothece comprises unicellular cyanobacteria that are morphologically diverse and ecologically versatile. Studies over the last decade have established members of this genus to be important components of the marine ecosystem, contributing significantly to the nitrogen and carbon cycle. System-lev...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00214-11
更新日期:2011-10-04 00:00:00
abstract:UNLABELLED:The ExPortal of Streptococcus pyogenes is a focal microdomain of the cytoplasmic membrane that clusters the translocons of the general secretory pathway with accessory factors to facilitate the maturation of secreted polypeptides. While it is known that the ExPortal is enriched in anionic lipids, the mechani...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00485-13
更新日期:2013-09-24 00:00:00
abstract::Since the discovery of symbioses between sulfur-oxidizing (thiotrophic) bacteria and invertebrates at hydrothermal vents over 40 years ago, it has been assumed that autotrophic fixation of CO2 by the symbionts drives these nutritional associations. In this study, we investigated "Candidatus Kentron," the clade of symb...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01112-19
更新日期:2019-06-25 00:00:00
abstract::The deep-sea tubeworm Riftia pachyptila lacks a digestive system but completely relies on bacterial endosymbionts for nutrition. Although the symbiont has been studied in detail on the molecular level, such analyses were unavailable for the animal host, because sequence information was lacking. To identify host-symbio...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02243-19
更新日期:2019-12-17 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) Tat binds the viral RNA structure transactivation-responsive element (TAR) and recruits transcriptional cofactors, amplifying viral mRNA expression. The Tat inhibitor didehydro-cortistatin A (dCA) promotes a state of persistent latency, refractory to viral reactivation. Here...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01750-18
更新日期:2019-07-02 00:00:00
abstract:UNLABELLED:Type III secretion systems (T3SS) translocate effector proteins into target cells in order to disrupt or modulate host cell signaling pathways and establish replicative niches. However, recognition of T3SS activity by cytosolic pattern recognition receptors (PRRs) of the nucleotide-binding domain leucine ric...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02095-14
更新日期:2015-02-17 00:00:00
abstract:UNLABELLED:Recent studies have established the fact that multiple members of the Rgg family of transcriptional regulators serve as key components of quorum sensing (QS) pathways that utilize peptides as intercellular signaling molecules. We previously described a novel QS system in Streptococcus pyogenes which utilizes...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00333-12
更新日期:2013-01-02 00:00:00
abstract::Paramyxoviruses are negative-sense single-stranded RNA viruses that comprise many important human and animal pathogens, including human parainfluenza viruses. These viruses bud from the plasma membrane of infected cells after the viral ribonucleoprotein complex (vRNP) is transported from the cytoplasm to the cell memb...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02028-20
更新日期:2020-08-25 00:00:00
abstract::In contrast to usual laboratory conditions, most bacteria in the human body grow in biofilms. Encased in a structured matrix, many pathogens display heightened resistance to antibiotics. Pseudomonas aeruginosa lung infections in cystic fibrosis patients represent a prime example of the clinical challenges that antibio...
journal_title:mBio
pub_type: 评论,杂志文章
doi:10.1128/mBio.00061-12
更新日期:2012-04-03 00:00:00
abstract:UNLABELLED:The acquisition, delivery, and incorporation of metals into their respective metalloproteins are important cellular processes. These processes are tightly controlled in order to prevent exposure of cells to free-metal concentrations that could yield oxidative damage. Copper (Cu) is one such metal that is req...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00293-11
更新日期:2012-01-31 00:00:00
abstract:UNLABELLED:As diet is considered the major regulator of the gut ecosystem, the overall objective of this work was to demonstrate that a detailed knowledge of the phytochemical composition of food could add to our understanding of observed changes in functionality and activity of the gut microbiota. We used metatranscri...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01263-15
更新日期:2015-10-27 00:00:00
abstract::The PCNA (proliferating cell nuclear antigen) ring plays central roles during DNA replication and repair. The yeast Elg1 RFC-like complex (RLC) is the principal unloader of chromatin-bound PCNA and thus plays a central role in maintaining genome stability. Here we identify a role for Elg1 in the unloading of PCNA duri...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01159-19
更新日期:2019-06-11 00:00:00
abstract:UNLABELLED:Pasteurella multocida toxin (PMT) induces atrophic rhinitis in animals, which is characterized by a degradation of nasal turbinate bones, indicating an effect of the toxin on bone cells such as osteoblasts and osteoclasts. The underlying molecular mechanism of PMT was defined as a persistent activation of he...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02190-14
更新日期:2014-11-11 00:00:00
abstract::The rapid increase of carbapenem resistance in Gram-negative bacteria has resurrected the importance of the polymyxin antibiotics. The recent discovery of plasmid-mediated polymyxin resistance (mcr-1) in carbapenem-resistant Enterobacteriaceae serves as an important indicator that the golden era of antibiotics is unde...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00540-17
更新日期:2017-07-25 00:00:00
abstract::Adult Drosophila melanogaster raised in the absence of symbiotic bacteria have fewer intestinal stem cell divisions and a longer life span than their conventionally reared counterparts. However, we do not know if increased stem cell divisions are essential for symbiont-dependent regulation of longevity. To determine i...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01114-18
更新日期:2018-07-31 00:00:00
abstract:UNLABELLED:In infection experiments with genetically distinct Mycobacterium tuberculosis complex (MTBC) strains, we identified clade-specific virulence patterns in human primary macrophages and in mice infected by the aerosol route, both reflecting relevant model systems. Exclusively human-adapted M. tuberculosis linea...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00250-13
更新日期:2013-07-30 00:00:00
abstract::This research analyzed six Aspergillus fumigatus genes encoding putative efflux proteins for their roles as transporters. TheA. fumigatus genes abcA, abcC, abcF, abcG, abcH, and abcI were cloned into plasmids and overexpressed in a Saccharomyces cerevisiae strain in which the highly active endogenous ABC transporter g...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00338-20
更新日期:2020-03-24 00:00:00
abstract::Cerebral malaria is a severe neurological complication associated with sequestration of Plasmodium falciparum-infected erythrocytes (IE) in the brain microvasculature, but the specific binding interactions remain under debate. Here, we have generated an engineered three-dimensional (3D) human brain endothelial microve...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00420-19
更新日期:2019-05-28 00:00:00
abstract::Many Gram-negative bacterial pathogens require a type 3 secretion system (T3SS) to deliver effector proteins into eukaryotic cells. Contact of the tip complex of the T3SS with a target eukaryotic cell initiates secretion of the two bacterial proteins that assemble into the translocon pore in the plasma membrane. The t...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00877-19
更新日期:2019-05-28 00:00:00
abstract::Burkholderia pseudomallei, the founding member of the B. pseudomallei complex (Bpc), is a biothreat agent and causes melioidosis, a disease whose treatment mainly relies on ceftazidime and meropenem. The concern is that B. pseudomallei could enhance its drug resistance repertoire by the acquisition of DNA from resista...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00592-20
更新日期:2020-04-14 00:00:00
abstract::Interindividual variation in the composition of the human gut microbiome was examined in relation to demographic and anthropometric traits, and to changes in dietary saturated fat intake and protein source. One hundred nine healthy men and women aged 21 to 65, with BMIs of 18 to 36, were randomized, after a two-week b...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01604-18
更新日期:2018-12-11 00:00:00
abstract:UNLABELLED:Helicobacter pylori chronically infects the gastric mucosa in more than half of the human population; in a subset of this population, its presence is associated with development of severe disease, such as gastric cancer. Genomic analysis of several strains has revealed an extensive H. pylori pan-genome, like...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00239-11
更新日期:2011-11-15 00:00:00
abstract:UNLABELLED:Enterococcus faecium, natively a gut commensal organism, emerged as a leading cause of multidrug-resistant hospital-acquired infection in the 1980s. As the living record of its adaptation to changes in habitat, we sequenced the genomes of 51 strains, isolated from various ecological environments, to understa...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00534-13
更新日期:2013-08-20 00:00:00
abstract::Transposon insertion sequencing (TIS) is a powerful high-throughput genetic technique that is transforming functional genomics in prokaryotes, because it enables genome-wide mapping of the determinants of fitness. However, current approaches for analyzing TIS data assume that selective pressures are constant over time...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01581-17
更新日期:2017-10-03 00:00:00
abstract:UNLABELLED:We studied the flocculation mechanism at the molecular level by determining the atomic structures of N-Flo1p and N-Lg-Flo1p in complex with their ligands. We show that they have similar ligand binding mechanisms but distinct carbohydrate specificities and affinities, which are determined by the compactness o...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00427-15
更新日期:2015-04-14 00:00:00
abstract:UNLABELLED:While research has identified an important contribution for metals, such as iron, in microbial pathogenesis, the roles of other transition metals, such as copper, remain mostly unknown. Recent evidence points to a requirement for copper homeostasis in the virulence of Cryptococcus neoformans based on a role ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00285-12
更新日期:2012-10-02 00:00:00
abstract:UNLABELLED:Competence for genetic transformation in Streptococcus pneumoniae develops in response to accumulation of a secreted peptide pheromone and was one of the initial examples of bacterial quorum sensing. Activation of this signaling system induces not only expression of the proteins required for transformation b...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00071-11
更新日期:2011-09-20 00:00:00