Abstract:
:Cellular sensing of bacterial RNA is increasingly recognized as a determinant of host-pathogen interactions. The intracellular pathogen Listeria monocytogenes induces high levels of type I interferons (alpha/beta interferons [IFN-α/β]) to create a growth-permissive microenvironment during infection. We previously demonstrated that RNAs secreted by L. monocytogenes (comprising the secRNome) are potent inducers of IFN-β. We determined the composition and diversity of the members of the secRNome and found that they are uniquely enriched for noncoding small RNAs (sRNAs). Testing of individual sRNAs for their ability to induce IFN revealed several sRNAs with this property. We examined ril32, an intracellularly expressed sRNA that is highly conserved for the species L. monocytogenes and that was the most potent inducer of IFN-β expression of all the sRNAs tested in this study, in more detail. The rli32-induced IFN-β response is RIG-I (retinoic acid inducible gene I) dependent, and cells primed with rli32 inhibit influenza virus replication. We determined the rli32 motif required for IFN induction. rli32 overproduction promotes intracellular bacterial growth, and a mutant lacking rli32 is restricted for intracellular growth in macrophages. rli32-overproducing bacteria are resistant to H2O2 and exhibit both increased catalase activity and changes in the cell envelope. Comparative transcriptome sequencing (RNA-Seq) analysis indicated that ril32 regulates expression of the lhrC locus, previously shown to be involved in cell envelope stress. Inhibition of IFN-β signaling by ruxolitinib reduced rli32-dependent intracellular bacterial growth, indicating a link between induction of the interferon system and bacterial physiology. rli32 is, to the best of our knowledge, the first secreted individual bacterial sRNA known to trigger the induction of the type I IFN response.IMPORTANCE Interferons are potent and broadly acting cytokines that stimulate cellular responses to nucleic acids of unusual structures or locations. While protective when induced following viral infections, the induction of interferons is detrimental to the host during L. monocytogenes infection. Here, we identify specific sRNAs, secreted by the bacterium, with the capacity to induce type I IFN. Further analysis of the most potent sRNA, rli32, links the ability to induce RIG-I-dependent induction of the type I IFN response to the intracellular growth properties of the bacterium. Our findings emphasize the significance of released RNA for Listeria infection and shed light on a compartmental strategy used by an intracellular pathogen to modulate host responses to its advantage.
journal_name
mBiojournal_title
mBioauthors
Frantz R,Teubner L,Schultze T,La Pietra L,Müller C,Gwozdzinski K,Pillich H,Hain T,Weber-Gerlach M,Panagiotidis GD,Mostafa A,Weber F,Rohde M,Pleschka S,Chakraborty T,Abu Mraheil Mdoi
10.1128/mBio.01223-19subject
Has Abstractpub_date
2019-10-08 00:00:00issue
5issn
2150-7511pii
mBio.01223-19journal_volume
10pub_type
杂志文章相关文献
mBio文献大全abstract::Pseudomonas aeruginosa is an opportunistic pathogen and the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. P. aeruginosa infections are difficult to treat due to a number of antibiotic resistance mechanisms and the organism's propensity to form multicellular biofilms. Epidemic strains of P....
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01828-18
更新日期:2019-01-29 00:00:00
abstract:UNLABELLED:The genus Cyanothece comprises unicellular cyanobacteria that are morphologically diverse and ecologically versatile. Studies over the last decade have established members of this genus to be important components of the marine ecosystem, contributing significantly to the nitrogen and carbon cycle. System-lev...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00214-11
更新日期:2011-10-04 00:00:00
abstract:UNLABELLED:Pseudomonas aeruginosa is a common cause of infection in the lungs of patients with cystic fibrosis (CF). In addition, biofilm formation and antibiotic resistance of Pseudomonas are major problems that can complicate antibiotic therapy. We evaluated the efficacy of using bacteriophages to kill the pathogen i...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00029-12
更新日期:2012-03-06 00:00:00
abstract::Reactive oxygen species (ROS) are produced by host phagocytes and exert antimicrobial actions against a broad range of pathogens. The observable antimicrobial actions of ROS are highly dependent on experimental conditions. This perspective reviews recent controversies regarding ROS in Salmonella-phagocyte interactions...
journal_title:mBio
pub_type: 杂志文章,评审
doi:10.1128/mBio.00141-11
更新日期:2011-09-06 00:00:00
abstract:UNLABELLED:Helicobacter pylori chronically infects the gastric mucosa in more than half of the human population; in a subset of this population, its presence is associated with development of severe disease, such as gastric cancer. Genomic analysis of several strains has revealed an extensive H. pylori pan-genome, like...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00239-11
更新日期:2011-11-15 00:00:00
abstract::We have previously identified Vibrio cholerae mutants in which the stress response to subinhibitory concentrations of aminoglycoside is altered. One gene identified, VC1636, encodes a putative DNA/RNA helicase, recently named RadD in Escherichia coli Here we combined extensive genetic characterization and high-through...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01173-19
更新日期:2019-07-02 00:00:00
abstract::Proteins that metabolize or bind the nucleotide second messenger cyclic diguanylate regulate a wide variety of important processes in bacteria. These processes include motility, biofilm formation, cell division, differentiation, and virulence. The role of cyclic diguanylate signaling in the lifestyle of Legionella pne...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00316-10
更新日期:2011-01-11 00:00:00
abstract::The yeast Yarrowia lipolytica is a potent accumulator of lipids, and lipogenesis in this organism can be influenced by a variety of factors, such as genetics and environmental conditions. Using a multifactorial study, we elucidated the effects of both genetic and environmental factors on regulation of lipogenesis in Y...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00857-17
更新日期:2017-06-20 00:00:00
abstract:UNLABELLED:The human pathogen Streptococcus pneumoniae (pneumococcus) exhibits a high degree of genomic diversity and plasticity. Isolates with high genomic similarity are grouped into lineages that undergo homologous recombination at variable rates. PMEN1 is a pandemic, multidrug-resistant lineage. Heterologous gene e...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00173-15
更新日期:2015-06-16 00:00:00
abstract::Host cytokine responses to Brucella abortus infection are elicited predominantly by the deployment of a type IV secretion system (T4SS). However, the mechanism by which the T4SS elicits inflammation remains unknown. Here we show that translocation of the T4SS substrate VceC into host cells induces proinflammatory resp...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00418-12
更新日期:2013-02-19 00:00:00
abstract::Biological nitrogen fixation is catalyzed by nitrogenase, a complex metalloenzyme found only in prokaryotes. N2 fixation is energetically highly expensive, and an energy-generating process such as photosynthesis can meet the energy demand of N2 fixation. However, synthesis and expression of nitrogenase are exquisitely...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01029-18
更新日期:2018-06-05 00:00:00
abstract::Opportunistic pathogens such as Candida species can use carboxylic acids, like acetate and lactate, to survive and successfully thrive in different environmental niches. These nonfermentable substrates are frequently the major carbon sources present in certain human body sites, and their efficient uptake by regulated ...
journal_title:mBio
pub_type: 杂志文章,评审
doi:10.1128/mBio.00156-20
更新日期:2020-05-12 00:00:00
abstract::The alarmone (p)ppGpp plays pivotal roles in basic bacterial stress responses by increasing tolerance of various nutritional limitations and chemical insults, including antibiotics. Despite intensive studies since (p)ppGpp was discovered over 4 decades ago, (p)ppGpp binding proteins have not been systematically identi...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02188-17
更新日期:2018-03-06 00:00:00
abstract::In Escherichia coli, FtsEX coordinates peptidoglycan (PG) synthesis and hydrolysis at the septum. It acts on FtsA in the cytoplasm to promote recruitment of septal PG synthetases and recruits EnvC, an activator of septal PG hydrolases, in the periplasm. Following recruitment, ATP hydrolysis by FtsEX is thought to regu...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01247-20
更新日期:2020-07-07 00:00:00
abstract:UNLABELLED:The nucleus has emerged as a key target for nucleomodulins, a family of effectors produced by bacterial pathogens to control host transcription or other nuclear processes. The virulence factor LntA from Listeria monocytogenes stimulates interferon responses during infection by inhibiting BAHD1, a nuclear pro...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00775-13
更新日期:2014-01-21 00:00:00
abstract:UNLABELLED:The gut microbiota enhances the host's metabolic capacity for processing nutrients and drugs and modulate the activities of multiple pathways in a variety of organ systems. We have probed the systemic metabolic adaptation to gut colonization for 20 days following exposure of axenic mice (n = 35) to a typical...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00271-10
更新日期:2011-03-01 00:00:00
abstract:UNLABELLED:Small colony variants (SCVs) are naturally occurring subpopulations of bacteria. The clinical characteristics and treatment outcomes of patients with prosthetic joint infection (PJI) caused by staphylococcal SCVs are unknown. This study was a retrospective series of 113 patients with staphylococcal PJI, with...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01910-14
更新日期:2014-09-30 00:00:00
abstract:UNLABELLED:Many viruses induce acute T cell-independent (TI) B cell responses due to their repetitive epitopes and the induction of innate cytokines. Nevertheless, T cell help is thought necessary for the development of long-lasting antiviral antibody responses in the form of long-lived plasma cells and memory B cells....
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00812-13
更新日期:2013-11-05 00:00:00
abstract::Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes blinding trachoma and sexually transmitted disease afflicting hundreds of millions of people globally. A fundamental but poorly understood pathophysiological characteristic of chlamydial infection is the propensity to cause persistent in...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01902-20
更新日期:2020-08-18 00:00:00
abstract:UNLABELLED:The increasing antibiotic resistance of pathogenic Escherichia coli species and the absence of a pan-protective vaccine pose major health concerns. We recently identified, by subtractive reverse vaccinology, nine Escherichia coli antigens that protect mice from sepsis. In this study, we characterized one of ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00010-12
更新日期:2012-04-10 00:00:00
abstract::An operon comprising two genes, CA_P0037 and CA_P0036, that encode proteins of unknown function that were previously shown to be highly expressed in acidogenic cells and repressed in solventogenic and alcohologenic cells is located on the pSOL1 megaplasmid of Clostridium acetobutylicum upstream of adhE2 A CA_P0037::in...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01218-16
更新日期:2016-10-04 00:00:00
abstract:UNLABELLED:Human noroviruses (HuNoVs) cause significant morbidity and mortality worldwide. However, despite substantial efforts, a small-animal model for HuNoV has not been described to date. Since "humanized" mice have been successfully used to study human-tropic pathogens in the past, we challenged BALB/c mice defici...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00450-13
更新日期:2013-07-16 00:00:00
abstract::Heme oxygenase-1 (HO-1) is a stress response antioxidant enzyme which catalyzes the degradation of heme released during inflammation. HO-1 expression is upregulated in both experimental and human Mycobacterium tuberculosis infection, and in patients it is a biomarker of active disease. Whether the enzyme plays a prote...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01675-16
更新日期:2016-10-25 00:00:00
abstract:UNLABELLED:Glycoside hydrolases (GHs) are key enzymes in the depolymerization of plant-derived cellulose, a process central to the global carbon cycle and the conversion of plant biomass to fuels and chemicals. A limited number of GH families hydrolyze crystalline cellulose, often by a processive mechanism along the ce...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01106-16
更新日期:2016-08-23 00:00:00
abstract:UNLABELLED:In the context of deciphering the metabolic strategies of the obligate pathogenic fungi in the genus Pneumocystis, the genomes of three species (P. carinii, P. murina, and P. jirovecii) were compared among themselves and with the free-living, phylogenetically related fission yeast (Schizosaccharomyces pombe)...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01834-14
更新日期:2014-11-04 00:00:00
abstract::Epidemiological studies on Streptococcus pneumoniae show that rates of carriage are highest in early childhood and that the major benefit of the pneumococcal conjugate vaccine (PCV) is a reduction in the incidence of nasopharyngeal colonization through decreased transmission within a population. In this study, we soug...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00188-17
更新日期:2017-03-14 00:00:00
abstract::The human fungal pathogen Candida albicans causes lethal systemic infections because of its ability to grow and disseminate in a host. The C. albicans plasma membrane is essential for virulence by acting as a protective barrier and through its key roles in interfacing with the environment, secretion of virulence facto...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00254-11
更新日期:2011-12-27 00:00:00
abstract::Ebola virus (EBOV) protein VP35 inhibits production of interferon alpha/beta (IFN) by blocking RIG-I-like receptor signaling pathways, thereby promoting virus replication and pathogenesis. A high-throughput screening assay, developed to identify compounds that either inhibit or bypass VP35 IFN-antagonist function, ide...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00368-17
更新日期:2017-04-04 00:00:00
abstract::Exudative cutaneous ulcers (CU) in yaws-endemic areas are associated with Treponema pallidum subsp. pertenue (TP) and Haemophilus ducreyi (HD), but one-third of CU cases are idiopathic (IU). Using mass drug administration (MDA) of azithromycin, a yaws eradication campaign on Lihir Island in Papua New Guinea reduced bu...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.03162-20
更新日期:2021-01-12 00:00:00
abstract::Emerging zoonotic viral diseases remain a challenge to global public health. Recent surveillance studies have implicated bats as potential reservoirs for a number of viral pathogens, including coronaviruses and Ebola viruses. Caliciviridae represent a major viral family contributing to emerging diseases in both human ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00869-18
更新日期:2018-05-22 00:00:00