Abstract:
:Kinases are involved in numerous critical cell signaling processes, and dysregulation in kinase signaling is implicated in many types of human cancers. In this study, we applied a parallel-reaction monitoring (PRM)-based targeted proteomic method to assess kinome reprogramming during melanoma metastasis in three pairs of matched primary/metastatic human melanoma cell lines. Around 300 kinases were detected in each pair of cell lines, and the results showed that Janus kinase 3 (JAK3) was with reduced expression in the metastatic lines of all three pairs of melanoma cells. Interrogation of The Cancer Genome Atlas (TCGA) data showed that reduced expression of JAK3 is correlated with poorer prognosis in melanoma patients. Additionally, metastatic human melanoma cells/tissues exhibited diminished levels of JAK3 mRNA relative to primary melanoma cells/tissues. Moreover, JAK3 suppresses the migration and invasion of cultured melanoma cells by modulating the activities of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9). In summary, our targeted kinome profiling method provided by far the most comprehensive dataset for kinome reprogramming associated with melanoma progression, which builds a solid foundation for examining the functions of other kinases in melanoma metastasis. Moreover, our results reveal a role of JAK3 as a potential suppressor for melanoma metastasis.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Miao W,Li L,Liu X,Qi TF,Guo L,Huang M,Wang Ydoi
10.1038/s41598-020-59572-5subject
Has Abstractpub_date
2020-02-12 00:00:00pages
2485issue
1issn
2045-2322pii
10.1038/s41598-020-59572-5journal_volume
10pub_type
杂志文章abstract::This work presents a new method for assisting in the identification process of missing persons in several contexts, such as enforced disappearances. We apply a Bayesian technique to incorporate non-genetic variables in the construction of prior information. In that way, we can learn from the already-solved cases of a ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59841-3
更新日期:2020-03-19 00:00:00
abstract::We successfully realised plasmon-driven selective reduction reactions of 2-amino-5-nitrobenzenethiol (2A-5-NBT) to 3,3'-dimercapto-4,4'-diaminoazobenzene , an azobenzene derivative, using surface-enhanced Raman scattering (SERS) spectroscopy, and supported by the theoretical calculations. The SERS spectra demonstrated...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10269
更新日期:2015-06-01 00:00:00
abstract::With approximately 1,500 extant species, freshwater crabs (Decapoda: Brachyura) are among the most diverse decapod crustaceans. Nevertheless, their fossil record is extremely limited: only Potamidae, Potamonautidae and Trichodactylidae are reported up to the Eocene of the Neotropics so far. This work documents unusual...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56180-w
更新日期:2019-12-27 00:00:00
abstract::Oxidative stress and mitochondrial dysfunction have been associated with metabolic and age-related diseases. Thus, the prevention of mitochondrial oxidative damage is nowadays a recognized pharmacological strategy to delay disease progression. Epidemiological studies suggested an association between the consumption of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07272-y
更新日期:2017-07-28 00:00:00
abstract::Sarcopenia is defined as skeletal muscle attenuation and has an association with metabolic syndrome. Metabolic syndrome, which includes obesity, is one of known predictive factors for gastroesophageal reflux disease (GERD). This study aimed to elucidate the association between sarcopenia and GERD. We retrospectively r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56702-6
更新日期:2019-12-27 00:00:00
abstract::The bacterial second messenger cyclic di-3',5'-guanosine monophosphate (c-di-GMP) is a key regulator of bacterial motility and virulence. As high levels of c-di-GMP are associated with the biofilm lifestyle, c-di-GMP hydrolysing phosphodiesterases (PDEs) have been identified as key targets to aid development of novel ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42166
更新日期:2017-02-10 00:00:00
abstract::Flavivirus-mediated inflammation causes neuronal death, but whether the infected neurons can evoke an innate immune response to elicit their own protection, is unknown. In an earlier study we have shown that neuronal RIG-I, play a significant role in inducing production and release of molecules that are related to inf...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00347
更新日期:2012-01-01 00:00:00
abstract::Egg oil from Portunus trituberculatus (Pt-egg oil) can overcome insulin resistance resulting from abundant bioactive lipids. However, its effects on obesity and gut microbiota were unclear. Here, we evaluated whether Pt-egg oil could improve obesity and gut microbiota or not in high-fat diet feeding mice. Results exhi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65199-3
更新日期:2020-05-21 00:00:00
abstract::Parthenocarpy is the development of an ovary into a seedless fruit without pollination. The ubiquitous downregulation of SlIAA9 induces not only parthenocarpic fruit formation but also an abnormal vegetative phenotype. To make parthenocarpic transgenic tomato plants without unwanted phenotypes, we found two genes, nam...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55400-7
更新日期:2019-12-11 00:00:00
abstract::Due to the high CO2-footprint of ordinary Portland cement (OPC), the search for alternative binders is now in a full swing. Rankinite-which is a hydraulically inactive material and low in calcium, is a real alternative to OPC, as it absorbs the harmful greenhouse gas from the air through carbonation hardening. Neverth...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71270-w
更新日期:2020-09-02 00:00:00
abstract::This study aimed to develop a cationic nanosuspension of chitosan (CS) and methoxy poly(ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL) for ocular delivery of diclofenac (DIC). MPEG-PCL-CS block polymer was synthesized by covalent coupling of MPEG-PCL with CS. The critical micelle concentration of the MPEG-PCL-CS blo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11337
更新日期:2015-06-12 00:00:00
abstract::Retinal diseases are frequently characterized by the accumulation of excessive scar tissue found throughout the neural retina. However, the pathophysiology of retinal fibrosis remains poorly understood, and the cell types that contribute to the fibrotic response are incompletely defined. Here, we show that myofibrobla...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72875-x
更新日期:2020-09-25 00:00:00
abstract::Iron oxide nanoparticles have attracted a great deal of research interest and have been widely used in bioscience and clinical research including as contrast agents for magnetic resonance imaging, hyperthermia and magnetic field assisted radionuclide therapy. It is therefore important to develop methods, which can pro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25852-4
更新日期:2018-05-10 00:00:00
abstract::We provide an explicit model for a spin-1/2 quasi-particle, based on the superposition of plasmon excitations in a quantum plasmas with intrinsic orbital angular momentum. Such quasi-particle solutions can show remarkable similarities with single electrons moving in vacuum: they have spin-1/2, a finite rest mass, and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26196-9
更新日期:2018-05-18 00:00:00
abstract::Soil erosion due to underground leakage is a major factor causing land degradation in karst regions. Rhizosphere effects (REs) on soil anti-erodibility (SAE) can alleviate this type of soil erosion by improving soil physical processes such as aggregate stability. However, the magnitudes and causes of interspecific var...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58784-z
更新日期:2020-02-12 00:00:00
abstract::In this work we study theoretically and experimentally the multi-particle structure of the so-called type-II quantum dots with spatially separated electrons and holes. Our calculations based on customarily developed full configuration interaction ap- proach reveal that exciton complexes containing holes interacting wi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45568
更新日期:2017-03-30 00:00:00
abstract::Surface plasmon polaritons (SPPs) and standing wave modes provide interesting and exotic properties for infrared metamaterial absorbers. Coupling of these modes promises further development in this field but restricted by the complexity of modes analysis. In this work, we investigate the general phenomenon of modes co...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46093
更新日期:2017-04-11 00:00:00
abstract::Image classification uses computers to simulate human understanding and cognition of images by automatically categorizing images. This study proposes a faster image classification approach that parallelizes the traditional Adaboost-Backpropagation (BP) neural network using the MapReduce parallel programming model. Fir...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38201
更新日期:2016-12-01 00:00:00
abstract::For one-dimensional (1d) nonlinear atomic lattices, the models with on-site nonlinearities such as the Frenkel-Kontorova (FK) and ϕ4 lattices have normal energy transport while the models with inter-site nonlinearities such as the Fermi-Pasta-Ulam-β (FPU-β) lattice exhibit anomalous energy transport. The 1d Discrete N...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23719-2
更新日期:2018-03-28 00:00:00
abstract::The RIβ subunit of cAMP-dependent protein kinase (PKA), encoded by Prkar1b, is a neuronal isoform of the type I regulatory subunit of PKA. Mice lacking the RIβ subunit exhibit normal long-term potentiation (LTP) in the Schaffer collateral pathway of the hippocampus and normal behavior in the open-field and fear condit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81515-x
更新日期:2021-01-21 00:00:00
abstract::The cysteine-rich PLAC8 domain of unknown function occurs in proteins found in most Eukaryotes. PLAC8-proteins play important yet diverse roles in different organisms, such as control of cell proliferation in animals and plants or heavy metal resistance in plants and fungi. Mammalian Onzin can be either pro-proliferat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43136-3
更新日期:2019-04-29 00:00:00
abstract::Oil palm (Elaeis guinensis Jacquin) is the most important source of vegetable oil and fat. Several linkage maps had been constructed using dominant and co-dominant markers to facilitate mapping of QTL. However, dominant markers are not easily transferable among different laboratories. We constructed a consensus linkag...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08232
更新日期:2015-02-04 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-60470-z
更新日期:2020-03-10 00:00:00
abstract::Systems of closely-spaced resonators can be strongly coupled by interactions mediated by scattered electromagnetic fields. In large systems the resulting response has been shown to be more sensitive to these collective interactions than to the detailed structure of individual resonators. Attempts to describe such syst...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41327-6
更新日期:2019-04-05 00:00:00
abstract::Efficient mitochondrial Ca2+ uptake takes place at contact points between the ER and mitochondria, and represents a key regulator of many cell functions. In a previous study with HeLa cells, we showed that ER-to-mitochondria Ca2+ transfer increases during the early phase of ER stress induced by tunicamycin as an adapt...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36394
更新日期:2016-11-03 00:00:00
abstract::Subsolid pulmonary nodules are commonly encountered in lung cancer screening and clinical routine. Compared to other nodule types, subsolid nodules are associated with a higher malignancy probability for which the size and mass of the nodule and solid core are important indicators. However, reliably measuring these ch...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-19101-3
更新日期:2018-01-12 00:00:00
abstract::CD47 is overexpressed in many human cancers, its level positively correlates with tumor invasion and metastasis. However, it is largely unknown whether CD47 overexpression drives metastasis and how CD47 lead to tumor metastasis in non-small cell lung cancer (NSCLC). In this study, we analyzed NSCLC specimens and cell ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29719
更新日期:2016-07-14 00:00:00
abstract::Gliomas are the most common brain tumors affecting the central nervous system and are associated with a high mortality rate. DCF1 is a membrane protein that was previously found to play a role in neural stem cell differentiation. In the present study, we found that overexpression of dcf1 significantly inhibited cell p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03702
更新日期:2014-01-15 00:00:00
abstract::Hybrid carbon films composed of graphene film and porous carbon film may give full play to the advantages of both carbon materials, and have great potential for application in energy storage and conversion devices. Unfortunately, there are very few reports on fabrication of hybrid carbon films. Here we demonstrate a s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07050
更新日期:2014-11-14 00:00:00
abstract::It is estimated from twin studies that heritable factors account for at-least half of asthma-risk, of which genetic variants identified through population studies explain only a small fraction. Multi-generation large families with high asthma prevalence can serve as a model to identify highly penetrant genetic variant...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54718-6
更新日期:2019-12-13 00:00:00