A Targeted Quantitative Proteomic Method Revealed a Substantial Reprogramming of Kinome during Melanoma Metastasis.


:Kinases are involved in numerous critical cell signaling processes, and dysregulation in kinase signaling is implicated in many types of human cancers. In this study, we applied a parallel-reaction monitoring (PRM)-based targeted proteomic method to assess kinome reprogramming during melanoma metastasis in three pairs of matched primary/metastatic human melanoma cell lines. Around 300 kinases were detected in each pair of cell lines, and the results showed that Janus kinase 3 (JAK3) was with reduced expression in the metastatic lines of all three pairs of melanoma cells. Interrogation of The Cancer Genome Atlas (TCGA) data showed that reduced expression of JAK3 is correlated with poorer prognosis in melanoma patients. Additionally, metastatic human melanoma cells/tissues exhibited diminished levels of JAK3 mRNA relative to primary melanoma cells/tissues. Moreover, JAK3 suppresses the migration and invasion of cultured melanoma cells by modulating the activities of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9). In summary, our targeted kinome profiling method provided by far the most comprehensive dataset for kinome reprogramming associated with melanoma progression, which builds a solid foundation for examining the functions of other kinases in melanoma metastasis. Moreover, our results reveal a role of JAK3 as a potential suppressor for melanoma metastasis.


Sci Rep


Scientific reports


Miao W,Li L,Liu X,Qi TF,Guo L,Huang M,Wang Y




Has Abstract


2020-02-12 00:00:00












  • The 3-D reconstruction of medieval wetland reclamation through electromagnetic induction survey.

    abstract::Studies of past human-landscape interactions rely upon the integration of archaeological, biological and geological information within their geographical context. However, detecting the often ephemeral traces of human activities at a landscape scale remains difficult with conventional archaeological field survey. Geop...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: De Smedt P,Van Meirvenne M,Herremans D,De Reu J,Saey T,Meerschman E,Crombé P,De Clercq W

    更新日期:2013-01-01 00:00:00

  • Trace Detection of RDX, HMX and PETN Explosives Using a Fluorescence Spot Sensor.

    abstract::1,3,5-trinitroperhydro-1,3,5-triazine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and pentaerythritol tetranitrate (PETN), the major components in plastic explosives, pose a significant threat to public safety. A quick, sensitive, and low-cost detection method for these non-volatile explosives is ea...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Wang C,Huang H,Bunes BR,Wu N,Xu M,Yang X,Yu L,Zang L

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    journal_title:Scientific reports

    pub_type: 杂志文章,随机对照试验


    authors: Slupsky CM,He X,Hernell O,Andersson Y,Rudolph C,Lönnerdal B,West CE

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    pub_type: 杂志文章


    authors: Guevara T,Körschgen H,Cuppari A,Schmitz C,Kuske M,Yiallouros I,Floehr J,Jahnen-Dechent W,Stöcker W,Gomis-Rüth FX

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    journal_title:Scientific reports

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    authors: Eacret JS,Gonzales DM,Franks RG,Burns JM Jr

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Dean KM,Fiolka R

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Parikh KS,Josyula A,Omiadze R,Ahn JY,Ha Y,Ensign LM,Hanes J,Pitha I

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    journal_title:Scientific reports

    pub_type: 杂志文章


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    journal_title:Scientific reports

    pub_type: 杂志文章


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    journal_title:Scientific reports

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    authors: San Román E,Vitrey A,Buencuerpo J,Prieto I,Llorens JM,García-Martín A,Alén B,Chaudhuri A,Neumann A,Brueck SR,Ripalda JM

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    journal_title:Scientific reports

    pub_type: 杂志文章


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    journal_title:Scientific reports

    pub_type: 杂志文章


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    journal_title:Scientific reports

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Kobayashi N,Ikeda K,Gu B,Takahashi S,Masumoto H,Maekawa S

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    pub_type: 杂志文章


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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Zurbuchen MA,Lake MP,Kohan SA,Leung B,Bouchard LS

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Chan RCK,Dai S,Lui SSY,Ho KKY,Hung KSY,Wang Y,Geng FL,Li Z,Cheung EFC

    更新日期:2015-07-02 00:00:00

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Sinigaglia L,Gracias S,Décembre E,Fritz M,Bruni D,Smith N,Herbeuval JP,Martin A,Dreux M,Tangy F,Jouvenet N

    更新日期:2018-07-18 00:00:00

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Zhang K,Kimball JS,Nemani RR,Running SW,Hong Y,Gourley JJ,Yu Z

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Kondili M,Fust A,Preussner J,Kuenne C,Braun T,Looso M

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Rahav E,Bar-Zeev E

    更新日期:2017-06-29 00:00:00