Abstract:
:Acute respiratory distress syndrome (ARDS), characterized by acute hypoxic respiratory dysfunction or failure, is a manifestation of multiple organ failure in the lung, and the most common risk factor is sepsis. We previously showed that blocking α2 -adrenoceptor (α2 -AR) could attenuate lung injury induced by endotoxin in rats. α2A -adrenoceptor (α2A -AR), a subtype of α2 -AR plays a key role in inflammatory diseases, but the mechanism remains unknown. Here, we explored the effect of BRL-44408 maleate (BRL), a specific α2A -AR antagonist, on cecal ligation puncture (CLP)-induced ARDS in rats and the underlying mechanism. Preadministration of BRL-44408 maleate significantly alleviated CLP-induced histological injury, macrophage infiltration, inflammatory response, and wet/dry ratio in lung tissue. However, there was no statistical difference in survival rate between the CLP and CLP+BRL groups. Extracellular regulated protein kinase (ERK1/2), p38MAPK, and p65 were activated in the CLP group, and BRL-44408 maleate inhibited the activation of these signal molecules, c-Jun N-terminal kinase (JNK) and protein kinase A (PKA) showed no changes in activation between these two groups. BRL-44408 maleate decreased lipopolysaccharide (LPS)-induced expression of cytokines in NR8383 rat alveolar macrophages and reduced phosphorylation of ERK1/2, p38MAPK, and p65. JNK and PKA were not influenced by LPS. Together, these findings suggest that antagonism of α2A -AR improves CLP-induced acute lung injury and involves the downregulation of ERK1/2, p38MAPK, and p65 pathway independent of the activation of JNK and PKA.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Cong Z,Li D,Tao Y,Lv X,Zhu Xdoi
10.1002/jcp.29586subject
Has Abstractpub_date
2020-10-01 00:00:00pages
6905-6914issue
10eissn
0021-9541issn
1097-4652journal_volume
235pub_type
杂志文章abstract::Malignant mesothelioma is an aggressive tumor of serosal surfaces, which is refractory to current treatment options. Arsenic trioxide (As2O3) is used clinically to treat acute promyelocytic leukemia, and also to inhibit proliferation of several solid tumors including hepatoma, esophageal, and gastric cancer in vitro. ...
journal_title:Journal of cellular physiology
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abstract::Epidermal growth factor (EGF) is a small protein that acts as a mitogen for various epidermal, epithelial, and fibroblastic cells that bear specific EGF receptors. The molecule that binds EGF is a 175-kD transmembrane protein, with an extracellular ligand binding domain and an intracellular domain that possesses tyros...
journal_title:Journal of cellular physiology
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abstract::Triple-negative breast cancer (TNBC) exhibits a higher level of glycolytic capacity and are commonly associated with an inflammatory microenvironment, but the regulatory mechanism and metabolic crosstalk between the tumor and tumor microenvironment (TME) are largely unresolved. Here, we show that glucose transporter 3...
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.24102
更新日期:2013-01-01 00:00:00
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.24518
更新日期:2014-07-01 00:00:00
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.1041500211
更新日期:1992-02-01 00:00:00
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更新日期:2003-09-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390311
更新日期:1989-06-01 00:00:00
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.1041620214
更新日期:1995-02-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/1097-4652(2000)9999:999<000::AID-JCP1039>3
更新日期:2001-03-01 00:00:00
abstract::Cardiac progenitor cells (CPCs) have the potential to differentiate into several cell lineages with the ability to restore in cardiac tissue. Multipotency and self-renewal activity are the crucial characteristics of CPCs. Also, CPCs have promising therapeutic roles in cardiac diseases such as valvular disease, thrombo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
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更新日期:2019-11-01 00:00:00
abstract::Differentiated embryo chondrocyte 1 (DEC1), a member of basic-helix-loop-helix transcription factor Bhlhe40, also called stimulated by retinoic acid 13, STRA13, plays an important role in the regulation of adipogenesis, tumorigenesis, peripheral circadian output, response to hypoxia, and development of metabolic syndr...
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更新日期:2018-12-01 00:00:00
abstract::Mitofusin 2 (MFN2) is a regulatory protein participating in mitochondria dynamics, cell proliferation, death, differentiation, and so on. This study aims at revealing the functional role of MFN2 in the pluripotency maintenance and primitive differetiation of embryonic stem cell (ESCs). A dox inducible silencing and ro...
journal_title:Journal of cellular physiology
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doi:10.1002/jcp.29020
更新日期:2020-02-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041330320
更新日期:1987-12-01 00:00:00
abstract::Interleukin (IL)-8 is released both in visceral adipose tissue and in contracting skeletal muscles. In this study, we examined cellular pathways associated with muscle hypertrophy, chosen on the basis of microRNA profiling, in differentiating rat primary skeletal muscle cells (RSkMC) treated with IL-8 (1 ng/ml) for 11...
journal_title:Journal of cellular physiology
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doi:10.1002/jcp.28568
更新日期:2019-11-01 00:00:00
abstract::Di- and tripeptides and peptide mimetics such as beta-lactam antibiotics are efficiently reabsorbed from the tubular lumen by a high-affinity peptide transporter. We have recently identified and characterized this H+-coupled high-affinity peptide transport system in the porcine proximal tubular cell line LLC-PK1. Here...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199903)178:3<341::AID-JCP8
更新日期:1999-03-01 00:00:00
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.27613
更新日期:2019-06-01 00:00:00
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.1041590306
更新日期:1994-06-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040870313
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更新日期:1986-09-01 00:00:00