当前位置： SCI文献检索 > JOURNAL OF NEUROCHEMISTRY期刊下所有文献 > Expression of α3β2β4 nicotinic acetylcholine receptors by rat adrenal chromaffin cells determined using novel conopeptide antagonists.

Expression of α3β2β4 nicotinic acetylcholine receptors by rat adrenal chromaffin cells determined using novel conopeptide antagonists.

Abstract：

:Adrenal chromaffin cells release neurotransmitters in response to stress and may be involved in conditions such as post-traumatic stress and anxiety disorders. Neurotransmitter release is triggered, in part, by activation of nicotinic acetylcholine receptors (nAChRs). However, despite decades of use as a model system for studying exocytosis, the nAChR subtypes involved have not been pharmacologically identified. Quantitative real-time PCR of rat adrenal medulla revealed an abundance of mRNAs for α3, α7, β2, and β4 subunits. Whole-cell patch-clamp electrophysiology of chromaffin cells and subtype-selective ligands were used to probe for nAChRs derived from the mRNAs found in adrenal medulla. A novel conopeptide antagonist, PeIA-5469, was created that is highly selective for α3β2 over other nAChR subtypes heterologously expressed in Xenopus laevis oocytes. Experiments using PeIA-5469 and the α3β4-selective α-conotoxin TxID revealed that rat adrenal medulla contain two populations of chromaffin cells that express either α3β4 nAChRs alone or α3β4 together with the α3β2β4 subtype. Conclusions were derived from observations that acetylcholine-gated currents in some cells were sensitive to inhibition by PeIA-5469 and TxID, while in other cells, currents were sensitive only to TxID. Expression of functional α7 nAChRs was determined using three α7-selective ligands: the agonist PNU282987, the positive allosteric modulator PNU120596, and the antagonist α-conotoxin [V11L,V16D]ArIB. The results of these studies identify for the first time the expression of α3β2β4 nAChRs as well as functional α7 nAChRs by rat adrenal chromaffin cells.

journal_name

J Neurochem

journal_title

Journal of neurochemistry

authors

Hone AJ,Rueda-Ruzafa L,Gordon TJ,Gajewiak J,Christensen S,Dyhring T,Albillos A,McIntosh JM

doi

10.1111/jnc.14966

subject

Has Abstract

pub_date

2020-07-01 00:00:00

pages

158-176

issue

2

eissn

0022-3042

issn

1471-4159

journal_volume

154

pub_type

杂志文章

相关文献

JOURNAL OF NEUROCHEMISTRY文献大全
  • Monoamine oxidase-A modulates apoptotic cell death induced by staurosporine in human neuroblastoma cells.

    abstract：:Monoamine oxidases (MAOs) are mitochondrial enzymes which control the levels of neurotransmitters in the brain and dietary amines in peripheral tissues via oxidative deamination. MAO has also been implicated in cell signalling. In this study, we describe the MAO-A isoform as functional in apoptosis induced by staurosp...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2007.04921.x

    authors： Fitzgerald JC,Ufer C,De Girolamo LA,Kuhn H,Billett EE

    更新日期：2007-12-01 00:00:00

  • Effects of ATP and taurine on calcium uptake by membrane preparations of the rat retina.

    abstract：:The effects of ATP and taurine on the kinetics of calcium uptake in rat retinal membrane preparations were determined. ATP increased calcium uptake at low calcium ion concentrations. Addition of ATP plus taurine further increased calcium uptake. Cooperative relationships were observed for calcium uptake in the absence...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1983.tb11296.x

    authors： Lombardini JB

    更新日期：1983-02-01 00:00:00

  • Thyroid hormone replacement restores circulating enkephalin concentrations in hypophysectomized fetal sheep.

    abstract：:We have investigated the effects of fetal hypophysectomy (HX) with or without thyroxine (T4)replacement on the plasma concentrations of free methionine-enkephalin (free Met-Enk), noradrenaline, and adrenaline in late gestation sheep fetus. Plasma adrenaline concentrations were significantly higher in intact fetal shee...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1994.63041386.x

    authors： Simonetta G,Young IR,McMillen IC

    更新日期：1994-10-01 00:00:00

  • Tetanus toxin inhibits depolarization-stimulated protein phosphorylation in rat cortical synaptosomes: effect on synapsin I phosphorylation and translocation.

    abstract：:Synapsin I, a prominent phosphoprotein in nerve terminals, is proposed to modulate exocytosis by interaction with the cytoplasmic surface of small synaptic vesicles and cytoskeletal elements in a phosphorylation-dependent manner. Tetanus toxin (TeTx), a potent inhibitor of neurotransmitter release, attenuated the depo...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1992.tb08445.x

    authors： Presek P,Jessen S,Dreyer F,Jarvie PE,Findik D,Dunkley PR

    更新日期：1992-10-01 00:00:00

  • A synaptosomal preparation from the guinea pig ileum myenteric plexus.

    abstract：:Our interest in investigating the presynaptic modulation of acetylcholine release led to the development of a synaptosomal preparation from the guinea pig ileum myenteric plexus-longitudinal muscle. A crude synaptosomal fraction (P2) was obtained by homogenization and differential centrifugation. The preparation exhib...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1981.tb01705.x

    authors： Briggs CA,Cooper JR

    更新日期：1981-03-01 00:00:00

  • Postmortem changes of normetanephrine determined by a mass-fragmentographic technique in different rat brain areas.

    abstract：:A specific and sensitive gas chromatographic-mass spectrometric assay for measurement of normetanephrine (NMN) in different rat brain regions is presented. The results show that hypothalamus contains the highest concentrations of the norepinephrine metabolite. An increase of NMN concentrations has been detected in ani...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1981.tb06334.x

    authors： Mocchetti I,De Angelis L,Racagni G

    更新日期：1981-12-01 00:00:00

  • Role of external and internal calcium on heterocarrier-mediated transmitter release.

    abstract：:Release-regulating heterocarriers exist on brain nerve endings. We have investigated in this study the mechanisms involved in the neurotransmitter release evoked by GABA heterocarrier activation. GABA increased the basal release of [3H]acetylcholine and [3H]noradrenaline from rat hippocampal synaptosomes and of [3H]do...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1996.66041468.x

    authors： Fassio A,Bonanno G,Fontana G,Usai C,Marchi M,Raiteri M

    更新日期：1996-04-01 00:00:00

  • Increased glutaminyl cyclase activity in brains of Alzheimer's disease individuals.

    abstract：:Glutaminyl cyclases (QC) catalyze the formation of neurotoxic pGlu-modified amyloid-β peptides found in the brains of people with Alzheimer's disease (AD). Reports of several-fold increases in soluble QC (sQC) expression in the brain and peripheral circulation of AD individuals has prompted the development of QC inhib...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.15114

    authors： Gunn AP,Wong BX,McLean C,Fowler C,Barnard PJ,Duce JA,Roberts BR,AIBL Research Group.

    更新日期：2020-07-02 00:00:00

  • Differential mechanisms of tolerance induced by NMDA and 3,5-dihydroxyphenylglycine (DHPG) preconditioning.

    abstract：:We investigated the molecular events triggered by NMDA and 3,5-dihydroxyphenylglycine (DHPG) preconditioning, that lead to neuroprotection against excitotoxic insults (AMPA or oxygen and glucose deprivation) in rat organotypic hippocampal slices, with particular attention on glutamate receptors and on cannabinoid syst...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.15033

    authors： Gerace E,Zianni E,Landucci E,Scartabelli T,Berlinguer Palmini R,Iezzi D,Moroni F,Di Luca M,Mannaioni G,Gardoni F,Pellegrini-Giampietro DE

    更新日期：2020-04-28 00:00:00

  • Hypoxia increases the susceptibility to oxidant stress and the permeability of the blood-brain barrier endothelial cell monolayer.

    abstract：:Using a cell culture model of the blood-brain barrier (BBB), we investigated the brain capillary endothelial cell (EC) response to hypoxia. The activities of antioxidant enzymes such as glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase and the GSH level of brain capillary ECs alone or i...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1995.65052138.x

    authors： Plateel M,Dehouck MP,Torpier G,Cecchelli R,Teissier E

    更新日期：1995-11-01 00:00:00

  • A comparison of the signalling properties of two tyramine receptors from Drosophila.

    abstract：:In invertebrates, the phenolamines, tyramine and octopamine, mediate many functional roles usually associated with the catecholamines, noradrenaline and adrenaline, in vertebrates. The α- and β-adrenergic classes of insect octopamine receptor are better activated by octopamine than tyramine. Similarly, the Tyramine 1 ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.12158

    authors： Bayliss A,Roselli G,Evans PD

    更新日期：2013-04-01 00:00:00

  • L-dopa inhibits complex IV of the electron transport chain in catecholamine-rich human neuroblastoma NB69 cells.

    abstract：:L-3,4-Dihydroxyphenylalanine (L-dopa) is toxic for human neuroblastoma cells NB69 and its toxicity is related to several mechanisms including quinone formation and enhanced production of free radicals related to the metabolism of dopamine via monoamine oxidase type B. We studied the effect of L-DOPA on activities of e...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1995.64020576.x

    authors： Pardo B,Mena MA,de Yébenes JG

    更新日期：1995-02-01 00:00:00

  • Activity-dependent phosphorylation of SNAP-25 in hippocampal organotypic cultures.

    abstract：:Synaptosomal-associated protein of 25 kDa (SNAP-25) is thought to play a key role in vesicle exocytosis and in the control of transmitter release. However, the precise mechanisms of action as well as the regulation of SNAP-25 remain unclear. Here we show by immunoprecipitation that activation of protein kinase C (PKC)...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1999.721699.x

    authors： Genoud S,Pralong W,Riederer BM,Eder L,Catsicas S,Muller D

    更新日期：1999-04-01 00:00:00

  • Regulation of axotomy-induced dopaminergic neuron death and c-Jun phosphorylation by targeted inhibition of cdc42 or mixed lineage kinase.

    abstract：:Mechanical transection of the nigrostriatal dopamine pathway at the medial forebrain bundle (MFB) results in the delayed degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNpc). We have previously demonstrated that c-Jun activation is an obligate component of neuronal death in this model. Her...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2005.03568.x

    authors： Crocker SJ,Hayley SP,Smith PD,Mount MP,Lamba WR,Callaghan SM,Slack RS,Park DS

    更新日期：2006-01-01 00:00:00

  • Two distinct regulatory mechanisms of neurotransmitter release by phosphatidylinositol 3-kinase.

    abstract：:Recent studies have indicated that various growth factors are involved in synaptic functions; however, the precise mechanisms remain unclear. In order to elucidate the molecular mechanisms of the growth factor-mediated regulation of presynaptic functions, the effects of epidermal growth factor (EGF) and insulin-like g...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2005.03242.x

    authors： Itakura M,Yamamori S,Kuwahara R,Sekiguchi M,Takahashi M

    更新日期：2005-07-01 00:00:00

  • Injury-induced "switch" from GTP-regulated to novel GTP-independent isoform of tissue transglutaminase in the rat spinal cord.

    abstract：:We recently found that alternative transcripts of tissue transglutaminase (tTG or TG2) were present in hippocampal brain regions of Alzheimer's disease (AD), but not in control, non-demented, age-matched brains. Since antecedent non-severe trauma has been implicated in AD and other neurodegenerative diseases, such as ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2002.00850.x

    authors： Festoff BW,SantaCruz K,Arnold PM,Sebastian CT,Davies PJ,Citron BA

    更新日期：2002-05-01 00:00:00

  • Simultaneous measurement of nitrite and nitrate levels as indices of nitric oxide release in the cerebellum of conscious rats.

    abstract：:We examined the modulation of nitric oxide production in vivo by measuring levels of nitrite (NO2-) and nitrate (NO3-) in the dialysate of the cerebellum in conscious rats, by using an in vivo brain microdialysis technique. The levels of both NO2- and NO3- were decreased by the intraperitoneal injection of NG-nitro-L-...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1997.68031234.x

    authors： Yamada K,Nabeshima T

    更新日期：1997-03-01 00:00:00

  • Functional effects of tau gene mutations deltaN296 and N296H.

    abstract：:Mutations in the tau gene cause frontotemporal dementia and parkinsonism linked to chromosome-17 (FTDP-17). Functionally, about half of the known mutations increase the alternative mRNA splicing of exon 10 of the tau gene, resulting in the overproduction of tau isoforms with four microtubule-binding repeats. The other...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.0022-3042.2001.00729.x

    authors： Yoshida H,Crowther RA,Goedert M

    更新日期：2002-02-01 00:00:00

  • Differential proteolysis of the full-length form of the L-type calcium channel alpha 1 subunit by calpain.

    abstract：:This study examines the proteolysis of the carboxy terminal domain of the full-length (alpha 1(212)) and truncated (alpha 1(190)) forms of the rabbit skeletal muscle L-type calcium channel alpha 1 subunit by calpain I and calpain II. Although both forms of the alpha 1 subunit show little sensitivity to proteolysis by ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1994.63041558.x

    authors： De Jongh KS,Colvin AA,Wang KK,Catterall WA

    更新日期：1994-10-01 00:00:00

  • Stimulus-evoked increase of glutamate in the mediorostral neostriatum/hyperstriatum ventrale of domestic chick after auditory filial imprinting: an in vivo microdialysis study.

    abstract：:Imprinting in chicks is a form of juvenile learning that has been used to study the basic cellular mechanisms of learning and memory. The forebrain area mediorostral neostriatum/hyperstriatum ventrale (MNH) is a center for acoustic imprinting. Electrophysiological and pharmacological behavioral studies in the MNH have...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1996.66031167.x

    authors： Gruss M,Braun K

    更新日期：1996-03-01 00:00:00

  • Membrane lipids, selectively diminished in Alzheimer brains, suggest synapse loss as a primary event in early-onset form (type I) and demyelination in late-onset form (type II).

    abstract：:Major membrane lipids were quantified in frontal (Brodmann area 9) and temporal (Brodmann areas 21 and 22) cortices, caudate nucleus, hippocampus, and frontal white matter of 12 cases with Alzheimer's disease (AD) type I (early onset), 21 cases with AD type II (late onset), and 20 age-matched controls. The concentrati...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1994.62031039.x

    authors： Svennerholm L,Gottfries CG

    更新日期：1994-03-01 00:00:00

  • SUMOylation of synaptic and synapse-associated proteins: An update.

    abstract：:SUMOylation is a post-translational modification that regulates protein signalling and complex formation by adjusting the conformation or protein-protein interactions of the substrate protein. There is a compelling and rapidly expanding body of evidence that, in addition to SUMOylation of nuclear proteins, SUMOylation...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章,评审

    doi：10.1111/jnc.15103

    authors： Henley JM,Seager R,Nakamura Y,Talandyte K,Nair J,Wilkinson KA

    更新日期：2020-06-15 00:00:00

  • Phosphorylation of neuronal kinesin heavy and light chains in vivo.

    abstract：:The microtubule-based motor protein kinesin is thought to drive anterograde organelle transport in axons, but nothing is known about how its force-generating activity or organelle-binding properties are regulated. Studies in other motility systems suggest that protein phosphorylation is a reasonable candidate for this...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1993.tb03513.x

    authors： Hollenbeck PJ

    更新日期：1993-06-01 00:00:00

  • Involvement of the AT1 receptor subtype in the effects of angiotensin IV and LVV-haemorphin 7 on hippocampal neurotransmitter levels and spatial working memory.

    abstract：:Intracerebroventricular (i.c.v.) administration of angiotensin IV (Ang IV) or Leu-Val-Val-haemorphin 7 (LVV-H7) improves memory performance in normal rats and reverses memory deficits in rat models for cognitive impairment. These memory effects were believed to be mediated via the putative 'AT4 receptor'. However, thi...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2009.06547.x

    authors： De Bundel D,Demaegdt H,Lahoutte T,Caveliers V,Kersemans K,Ceulemans AG,Vauquelin G,Clinckers R,Vanderheyden P,Michotte Y,Smolders I

    更新日期：2010-03-01 00:00:00

  • Retrograde axonal transport of gangliosides and glycoproteins in the motoneurons of rat sciatic nerve.

    abstract：:Axonal transport of glycoconjugates was studied in the motoneurons of rat sciatic nerve following injection of [3H]glucosamine into the lumbosacral spinal cord. After varying time intervals, the sciatic nerve was exposed, and two ligatures were tied for collection of materials undergoing anterograde and retrograde tra...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1985.tb05551.x

    authors： Aquino DA,Bisby MA,Ledeen RW

    更新日期：1985-10-01 00:00:00

  • Mutant copper-zinc superoxide dismutase associated with amyotrophic lateral sclerosis binds to adenine/uridine-rich stability elements in the vascular endothelial growth factor 3'-untranslated region.

    abstract：:Vascular endothelial growth factor (VEGF) is a neurotrophic factor essential for maintenance of motor neurons. Loss of this factor produces a phenotype similar to amyotrophic lateral sclerosis (ALS). We recently showed that ALS-producing mutations of Cu/Zn-superoxide dismutase (SOD1) disrupt post-transcriptional regul...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2008.05856.x

    authors： Li X,Lu L,Bush DJ,Zhang X,Zheng L,Suswam EA,King PH

    更新日期：2009-02-01 00:00:00

  • Studies on subcellular fractions which are involved in myelin assembly: labeling of myelin proteins by a double radioisotope approach indicates developmental relationships.

    abstract：:The question of developmental relationships amongst myelin-related membranes in subfractions of myelinating mouse brain (15 days) was investigated by a time-staggered double isotope protocol using [3H]leucine and [14C]leucine. Preliminary results are interpreted and discussed in the context of a mathematical conceptua...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1983.tb09041.x

    authors： Pereyra PM,Braun PE,Greenfield S,Hogan EL

    更新日期：1983-10-01 00:00:00

  • Dopamine neuronal transport kinetics and effects of amphetamine.

    abstract：:The dopamine (DA) transporter (DAT) regulates DA neurotransmission by recycling DA back into neurons. Drugs that interfere with DAT function, e.g., cocaine and amphetamine, can have profound behavioral effects. The kinetics of DA transport by DAT in isolated synaptosomal or single cell preparations have been previousl...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1999.0732406.x

    authors： Jones SR,Joseph JD,Barak LS,Caron MG,Wightman RM

    更新日期：1999-12-01 00:00:00

  • Distribution of preproatrial natriuretic peptide mRNA in rat brain detected by in situ hybridization of DNA oligonucleotides: enrichment in hypothalamic and limbic regions.

    abstract：:The expression and distribution of mRNA encoding preproatrial natriuretic peptide (ppANP) in rat brain has been investigated by in situ hybridization of two 35S-labeled synthetic DNA oligonucleotides, based on a cDNA clone sequence that encodes rat ppANP. The highest relative concentrations of ppANP mRNA were detected...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1992.tb09434.x

    authors： Gundlach AL,Knobe KE

    更新日期：1992-08-01 00:00:00

  • Posttranslational modifications of nuclear proteins in rat cerebral hemispheres during postnatal development.

    abstract：:The processes of acetylation, phosphorylation, and methylation of nuclear proteins in cerebral hemispheres of 10- and 30-day-old rats were investigated. The experiments were carried out in vitro by measuring the incorporation of labeled precursors into histones and nonhistone chromosomal proteins (NHP) extracted from ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1983.tb08041.x

    authors： Serra I,Kamiyama M,Hashim GA,Ragonese P,Giuffrida AM

    更新日期：1983-03-01 00:00:00